##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549364_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85000
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.879529411764707	31.0	31.0	34.0	30.0	34.0
2	32.1534	33.0	31.0	34.0	30.0	34.0
3	31.902623529411766	33.0	31.0	34.0	30.0	34.0
4	35.752729411764705	37.0	35.0	37.0	35.0	37.0
5	28.412541176470587	37.0	32.0	37.0	0.0	37.0
6	32.02522352941177	37.0	32.0	37.0	17.0	37.0
7	35.01405882352941	37.0	33.0	37.0	32.0	37.0
8	35.65114117647059	37.0	35.0	37.0	35.0	37.0
9	37.70350588235294	39.0	38.0	39.0	35.0	39.0
10	37.35471764705883	39.0	37.0	39.0	34.0	39.0
11	37.39805882352941	39.0	37.0	39.0	34.0	39.0
12	37.20178823529412	39.0	37.0	39.0	34.0	39.0
13	36.8207294117647	39.0	37.0	39.0	33.0	39.0
14	38.306623529411766	40.0	37.0	41.0	33.0	41.0
15	38.4664	40.0	38.0	41.0	34.0	41.0
16	38.44230588235294	40.0	38.0	41.0	34.0	41.0
17	38.38384705882353	40.0	37.0	41.0	34.0	41.0
18	38.21497647058823	40.0	37.0	41.0	34.0	41.0
19	38.25189411764706	40.0	37.0	41.0	34.0	41.0
20	38.225564705882356	40.0	36.0	41.0	34.0	41.0
21	38.16575294117647	40.0	36.0	41.0	34.0	41.0
22	38.018870588235295	40.0	36.0	41.0	34.0	41.0
23	38.0286	40.0	36.0	41.0	34.0	41.0
24	37.96121176470588	40.0	36.0	41.0	34.0	41.0
25	37.8507294117647	40.0	36.0	41.0	33.0	41.0
26	37.737035294117646	40.0	36.0	41.0	33.0	41.0
27	37.6073294117647	40.0	35.0	41.0	33.0	41.0
28	37.568482352941174	40.0	36.0	41.0	33.0	41.0
29	37.54170588235294	40.0	36.0	41.0	33.0	41.0
30	37.34967058823529	39.0	35.0	41.0	33.0	41.0
31	37.25350588235294	39.0	35.0	41.0	33.0	41.0
32	37.04510588235294	39.0	35.0	40.0	32.0	41.0
33	36.8362	39.0	35.0	40.0	31.0	41.0
34	36.55344705882353	39.0	35.0	40.0	31.0	41.0
35	36.305176470588236	39.0	35.0	40.0	30.0	41.0
36	36.22154117647059	39.0	35.0	40.0	30.0	41.0
37	36.10038823529412	39.0	35.0	40.0	30.0	41.0
38	35.93654117647059	39.0	35.0	40.0	29.0	41.0
39	35.81544705882353	39.0	35.0	40.0	29.0	41.0
40	35.64825882352941	38.0	35.0	40.0	27.0	41.0
41	35.50765882352941	38.0	35.0	40.0	27.0	41.0
42	35.44095294117647	38.0	35.0	40.0	26.0	41.0
43	35.300317647058826	38.0	35.0	40.0	26.0	41.0
44	35.1118	38.0	35.0	40.0	25.0	41.0
45	35.02170588235294	38.0	34.0	40.0	25.0	41.0
46	34.923658823529415	38.0	34.0	40.0	24.0	41.0
47	34.77824705882353	38.0	34.0	40.0	24.0	41.0
48	34.69414117647059	38.0	34.0	40.0	24.0	41.0
49	34.59996470588235	38.0	34.0	40.0	24.0	40.0
50	34.50022352941176	38.0	34.0	40.0	24.0	40.0
51	34.7466	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	3.0
16	2.0
17	9.0
18	20.0
19	39.0
20	99.0
21	146.0
22	230.0
23	365.0
24	540.0
25	942.0
26	1239.0
27	1355.0
28	1370.0
29	1437.0
30	1595.0
31	1928.0
32	2439.0
33	3378.0
34	5007.0
35	7073.0
36	7145.0
37	12048.0
38	17681.0
39	18906.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.82588235294118	24.748235294117645	29.43529411764706	16.99058823529412
2	30.670588235294115	26.285882352941176	30.84470588235294	12.198823529411765
3	27.647058823529413	25.174117647058825	35.178823529411765	12.0
4	25.388235294117646	27.082352941176467	34.16823529411765	13.361176470588235
5	17.981176470588238	46.14705882352941	25.094117647058823	10.77764705882353
6	20.662352941176472	41.41882352941176	28.199999999999996	9.718823529411765
7	83.54588235294118	4.472941176470589	9.943529411764706	2.0376470588235294
8	83.14117647058823	5.083529411764706	9.632941176470588	2.142352941176471
9	76.20823529411764	9.618823529411765	10.79764705882353	3.375294117647059
10	24.86470588235294	49.322352941176476	16.745882352941177	9.067058823529411
11	27.570588235294117	25.212941176470586	32.432941176470585	14.783529411764707
12	28.418823529411764	24.065882352941177	31.574117647058824	15.941176470588236
13	21.560000000000002	28.996470588235297	31.738823529411764	17.704705882352943
14	15.474117647058824	31.836470588235294	33.14588235294117	19.543529411764705
15	15.448235294117646	31.365882352941178	37.374117647058824	15.811764705882354
16	19.19529411764706	30.03411764705882	35.12705882352941	15.643529411764707
17	17.70235294117647	30.425882352941176	32.78823529411765	19.083529411764705
18	17.596470588235295	31.050588235294118	35.47176470588235	15.881176470588235
19	21.145882352941175	29.90470588235294	31.99529411764706	16.954117647058823
20	21.26	30.003529411764706	34.362352941176475	14.374117647058823
21	19.992941176470588	30.18	34.55529411764706	15.271764705882354
22	20.511764705882353	26.65764705882353	33.82235294117647	19.008235294117647
23	17.61529411764706	30.09294117647059	34.81647058823529	17.47529411764706
24	15.57294117647059	29.395294117647058	39.32823529411765	15.703529411764706
25	16.172941176470587	33.8635294117647	33.87529411764706	16.08823529411765
26	16.43764705882353	35.19529411764706	31.582352941176474	16.78470588235294
27	16.516470588235293	34.87294117647059	32.70823529411765	15.90235294117647
28	14.974117647058824	33.20235294117647	36.22470588235294	15.598823529411765
29	15.858823529411763	29.24	36.427058823529414	18.474117647058826
30	16.114117647058823	33.050588235294114	34.16117647058823	16.67411764705882
31	19.485882352941175	31.631764705882354	32.790588235294116	16.09176470588235
32	19.018823529411765	31.168235294117647	33.921176470588236	15.891764705882352
33	18.96470588235294	31.476470588235294	32.334117647058825	17.224705882352943
34	17.27764705882353	30.181176470588234	33.43411764705883	19.107058823529414
35	19.05294117647059	29.042352941176468	33.88941176470588	18.01529411764706
36	20.68	31.51529411764706	31.423529411764704	16.381176470588237
37	16.43529411764706	34.072941176470586	33.07058823529412	16.421176470588236
38	16.54705882352941	33.59294117647059	31.95529411764706	17.904705882352943
39	17.72823529411765	32.368235294117646	32.589411764705886	17.314117647058826
40	18.90235294117647	31.171764705882353	32.451764705882354	17.474117647058822
41	17.155294117647056	30.70235294117647	32.519999999999996	19.622352941176473
42	16.409411764705883	30.79647058823529	32.44235294117647	20.351764705882353
43	17.988235294117647	30.584705882352942	32.290588235294116	19.136470588235294
44	16.30235294117647	32.358823529411765	32.25058823529412	19.08823529411765
45	15.931764705882353	34.88235294117647	30.561176470588236	18.62470588235294
46	16.501176470588234	35.13294117647059	30.729411764705883	17.636470588235294
47	17.763529411764704	30.867058823529415	32.777647058823526	18.59176470588235
48	17.261176470588236	30.470588235294116	34.81294117647059	17.455294117647057
49	17.244705882352942	31.39294117647059	33.19529411764706	18.167058823529413
50	16.905882352941177	32.796470588235294	32.67294117647059	17.62470588235294
51	17.49529411764706	30.608235294117648	32.35529411764706	19.541176470588233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	130.5
2	170.0
3	966.5
4	1763.0
5	1220.5
6	678.0
7	654.0
8	630.0
9	623.0
10	616.0
11	627.5
12	639.0
13	625.5
14	612.0
15	568.5
16	525.0
17	534.5
18	544.0
19	508.5
20	473.0
21	587.0
22	701.0
23	812.5
24	924.0
25	1177.5
26	1761.0
27	2091.0
28	2598.0
29	3105.0
30	3548.5
31	3992.0
32	4460.5
33	4929.0
34	5317.0
35	5705.0
36	6013.0
37	6321.0
38	6401.0
39	6481.0
40	6871.0
41	7261.0
42	7430.0
43	7599.0
44	7202.0
45	6805.0
46	6408.0
47	6011.0
48	5293.0
49	4575.0
50	3953.0
51	3331.0
52	2739.5
53	2148.0
54	1716.5
55	1285.0
56	1156.5
57	1028.0
58	924.0
59	820.0
60	740.0
61	660.0
62	552.5
63	445.0
64	352.0
65	259.0
66	204.0
67	149.0
68	114.5
69	80.0
70	66.5
71	53.0
72	44.5
73	36.0
74	26.0
75	12.0
76	8.0
77	7.0
78	6.0
79	3.5
80	1.0
81	1.0
82	1.0
83	1.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	85000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48352941176471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9409538513679	47.45058823529412
2	11.58517068027339	14.477647058823528
3	5.155240910545838	9.663529411764706
4	2.775319613639359	6.936470588235294
5	1.8546063903899381	5.794117647058823
6	1.0449812656511834	3.9176470588235293
7	0.6382858541545066	2.791764705882353
8	0.38410122196908364	1.92
9	0.2108791022575361	1.1858823529411766
>10	0.38786692022368247	3.4258823529411764
>50	0.011297094763796577	0.49176470588235294
>100	0.009414245636497147	0.9188235294117646
>500	0.0018828491272994295	1.0258823529411765
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	872	1.0258823529411765	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	209	0.24588235294117647	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	170	0.2	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	151	0.1776470588235294	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	126	0.14823529411764708	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	125	0.14705882352941177	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001176470588235294	0.0	0.0	0.04470588235294118	0.0
2	0.001176470588235294	0.0	0.0	0.1411764705882353	0.0
3	0.001176470588235294	0.0	0.0	0.18941176470588236	0.0
4	0.001176470588235294	0.0	0.0	0.2564705882352941	0.0
5	0.001176470588235294	0.0	0.0	0.4811764705882353	0.0
6	0.001176470588235294	0.0	0.0	0.6247058823529412	0.0
7	0.001176470588235294	0.0	0.0	0.7494117647058823	0.0
8	0.001176470588235294	0.0	0.0	0.9341176470588235	0.0
9	0.001176470588235294	0.0	0.0	1.0058823529411764	0.0
10	0.001176470588235294	0.0	0.0	1.3082352941176472	0.0
11	0.001176470588235294	0.0	0.0	1.5023529411764707	0.0
12	0.001176470588235294	0.0	0.0	1.7741176470588236	0.0
13	0.001176470588235294	0.0	0.0	1.8552941176470588	0.0
14	0.001176470588235294	0.0	0.0	1.8952941176470588	0.0
15	0.001176470588235294	0.0	0.0	1.9623529411764706	0.0
16	0.001176470588235294	0.0	0.0	2.071764705882353	0.0
17	0.001176470588235294	0.0	0.0	2.2023529411764704	0.0
18	0.001176470588235294	0.0	0.0	2.355294117647059	0.0
19	0.001176470588235294	0.0	0.0	2.4447058823529413	0.0
20	0.001176470588235294	0.0	0.0	2.548235294117647	0.0
21	0.001176470588235294	0.0	0.0	2.6788235294117646	0.0
22	0.001176470588235294	0.0	0.0	2.8117647058823527	0.0
23	0.001176470588235294	0.0	0.0	2.938823529411765	0.0
24	0.001176470588235294	0.0	0.0	3.050588235294118	0.0
25	0.001176470588235294	0.0	0.0	3.1470588235294117	0.0
26	0.001176470588235294	0.0	0.0	3.264705882352941	0.0
27	0.001176470588235294	0.0	0.0	3.356470588235294	0.0
28	0.001176470588235294	0.0	0.0	3.4658823529411764	0.0
29	0.001176470588235294	0.0	0.0	3.5976470588235294	0.0
30	0.001176470588235294	0.0	0.0	3.7388235294117647	0.0
31	0.001176470588235294	0.0	0.0	3.8858823529411763	0.0
32	0.001176470588235294	0.0	0.0	4.041176470588235	0.0
33	0.001176470588235294	0.0	0.0	4.181176470588236	0.0
34	0.001176470588235294	0.0	0.0	4.327058823529412	0.0
35	0.001176470588235294	0.0	0.0	4.496470588235294	0.0
36	0.001176470588235294	0.0	0.0	4.669411764705882	0.0
37	0.001176470588235294	0.0	0.0	4.850588235294118	0.0
38	0.001176470588235294	0.0	0.0	5.004705882352941	0.0
39	0.001176470588235294	0.0	0.0	5.185882352941176	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAC	35	1.1942575E-7	45.000004	5
CTTGGTC	35	1.1942575E-7	45.000004	29
CGGGATC	35	1.1942575E-7	45.000004	6
GACGGGA	75	0.0	45.000004	4
AGGGACC	70	0.0	45.000004	6
ATGGGCT	45	3.765308E-10	45.0	5
CTATGGG	45	3.765308E-10	45.0	3
ACACGGG	20	6.99644E-4	45.0	3
CGCGGGA	20	6.99644E-4	45.0	4
CGACGGG	25	3.860296E-5	45.0	3
ATGGCCT	25	3.860296E-5	45.0	11
TGGACGG	20	6.99644E-4	45.0	2
TATGGCC	25	3.860296E-5	45.0	10
GACCGAT	20	6.99644E-4	45.0	9
ATAGCGG	20	6.99644E-4	45.0	2
GGATCTA	25	3.860296E-5	45.0	8
GCACGGG	20	6.99644E-4	45.0	3
AATGGGC	20	6.99644E-4	45.0	4
AGGGTGC	20	6.99644E-4	45.0	6
TAACGGG	20	6.99644E-4	45.0	3
>>END_MODULE