FastQCFastQC Report
Sat 18 Jun 2016
SRR3549363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33965
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.8243780362137495No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.3680259090239953No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA590.17370822905932579No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT410.12071249815987047No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG370.1089356690711026No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG370.1089356690711026No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGAT206.9368887E-445.05
GGATGGC253.8111324E-545.08
GGATGGA206.9368887E-445.08
GGATGAC253.8111324E-545.08
TACGGGA206.9368887E-445.04
GCAGGGA406.4846972E-945.04
GATTGGG206.9368887E-445.03
ATAATGG206.9368887E-445.02
CGAGGGA206.9368887E-445.04
GATCTTT206.9368887E-445.09
AGCGGGA206.9368887E-445.04
CGGGATG206.9368887E-445.06
CGGGACA253.8111324E-545.06
TGTAGGG502.0008883E-1145.03
ACGGGAG253.8111324E-545.05
CTAAAGC206.9368887E-445.05
AAGGATG406.4846972E-945.06
GTAGGGC351.1660268E-745.04
ACTAGGA206.9368887E-445.04
ACCTATG253.8111324E-545.034