##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33965
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679228617694687	31.0	31.0	33.0	30.0	34.0
2	32.040688944501696	33.0	31.0	34.0	30.0	34.0
3	31.905432062417194	33.0	31.0	34.0	30.0	34.0
4	35.75757397320771	37.0	35.0	37.0	35.0	37.0
5	35.78383630207566	37.0	35.0	37.0	35.0	37.0
6	35.923656705432066	37.0	35.0	37.0	35.0	37.0
7	22.94785808920948	35.0	0.0	37.0	0.0	37.0
8	28.971735610186958	35.0	17.0	37.0	17.0	37.0
9	35.317385543942294	37.0	32.0	39.0	32.0	39.0
10	36.50393051670838	37.0	35.0	39.0	32.0	39.0
11	37.189076991020166	39.0	37.0	39.0	34.0	39.0
12	37.226438981304284	39.0	37.0	39.0	34.0	39.0
13	37.08741351391138	39.0	37.0	39.0	33.0	39.0
14	38.52430443103194	40.0	38.0	41.0	34.0	41.0
15	38.61098189312528	40.0	38.0	41.0	34.0	41.0
16	38.59464154276461	40.0	38.0	41.0	34.0	41.0
17	38.631061386721626	40.0	38.0	41.0	34.0	41.0
18	38.52147799205064	40.0	38.0	41.0	34.0	41.0
19	38.481525099367	40.0	37.0	41.0	34.0	41.0
20	38.38080376858531	40.0	37.0	41.0	34.0	41.0
21	38.31682614456058	40.0	37.0	41.0	34.0	41.0
22	38.22870602090387	40.0	37.0	41.0	34.0	41.0
23	38.202561460326805	40.0	37.0	41.0	34.0	41.0
24	38.07893419696747	40.0	36.0	41.0	34.0	41.0
25	37.88888561754747	40.0	36.0	41.0	33.0	41.0
26	37.786073899602535	40.0	36.0	41.0	33.0	41.0
27	37.720594729868985	40.0	36.0	41.0	33.0	41.0
28	37.743324010010305	40.0	36.0	41.0	33.0	41.0
29	37.57211835713234	40.0	36.0	41.0	33.0	41.0
30	37.421109966141614	39.0	36.0	40.0	33.0	41.0
31	37.22013837774179	39.0	36.0	40.0	33.0	41.0
32	37.02961872515825	39.0	35.0	40.0	32.0	41.0
33	36.82408361548653	39.0	35.0	40.0	31.0	41.0
34	36.49768879729133	39.0	35.0	40.0	30.0	41.0
35	36.29907257470926	39.0	35.0	40.0	30.0	41.0
36	36.12159576034153	39.0	35.0	40.0	30.0	41.0
37	36.089827763874574	39.0	35.0	40.0	30.0	41.0
38	35.879493596349185	38.0	35.0	40.0	29.0	41.0
39	35.700574120418075	38.0	35.0	40.0	29.0	41.0
40	35.48667746209333	38.0	35.0	40.0	28.0	41.0
41	35.357308994553215	38.0	35.0	40.0	27.0	41.0
42	35.33481525099367	38.0	35.0	40.0	27.0	41.0
43	35.15748564698955	38.0	35.0	40.0	27.0	40.0
44	34.85705873693508	38.0	34.0	40.0	25.0	40.0
45	34.8057706462535	38.0	34.0	40.0	25.0	40.0
46	34.71482408361549	38.0	34.0	40.0	25.0	40.0
47	34.55015457088179	38.0	34.0	40.0	24.0	40.0
48	34.39846901221846	37.0	34.0	39.0	24.0	40.0
49	34.128014132194906	37.0	33.0	39.0	24.0	40.0
50	34.17088179007802	37.0	33.0	39.0	24.0	40.0
51	34.59673192992787	38.0	34.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	3.0
17	5.0
18	9.0
19	13.0
20	39.0
21	71.0
22	83.0
23	142.0
24	220.0
25	386.0
26	438.0
27	526.0
28	549.0
29	584.0
30	593.0
31	742.0
32	1015.0
33	1372.0
34	2141.0
35	2939.0
36	3735.0
37	7139.0
38	7431.0
39	3789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.221257176505226	24.478139261003975	29.786544972766084	16.514058589724716
2	30.784631238039157	26.147504784336817	30.943618430737523	12.1242455468865
3	27.59016634771088	25.243633151773885	35.10083909907257	12.065361401442662
4	25.620491682614453	27.248638304136612	33.970263506550864	13.160606506698072
5	22.364198439570146	32.47755041954954	31.338142205211245	13.82010893566907
6	20.712498159870453	42.119829235978216	27.8757544531135	9.291918151037832
7	54.50610923008979	37.99499484763727	6.238775209774769	1.26012071249816
8	85.23185632268512	4.348594141027529	8.94450169291918	1.4750478433681733
9	77.86544972766083	9.244810834682763	10.06624466362432	2.823494774032092
10	25.540998086265272	49.79832180185485	16.148976887972914	8.511703223906963
11	28.21728249668777	24.955100839099075	32.02414249963198	14.803474164581187
12	28.829677609303694	24.01001030472545	31.155601354335342	16.00471073163551
13	21.33372589430296	29.19181510378331	31.467687325187693	18.00677167672604
14	15.345208302664506	31.794494332401	33.21949065214191	19.640806712792582
15	15.43942293537465	31.38230531429413	37.485646989548066	15.692624760783158
16	19.107905196525834	30.2193434417783	34.89180038274694	15.780950978948919
17	17.264831444133666	30.740468128956277	32.383335786839396	19.61136464007066
18	17.515089062269983	31.326365376122478	35.35109671720889	15.807448844398644
19	20.956867363462386	29.833652289121154	31.94464890328279	17.264831444133666
20	21.21890181068747	30.063300456352128	34.75931105549831	13.958486677462092
21	19.631974090976005	30.646253496246135	34.60621227734432	15.115560135433533
22	20.23848078904755	26.824672456940966	34.26174002649787	18.675106727513615
23	17.70057412041808	29.963197409097603	34.9830708081849	17.353157662299427
24	15.071397026350656	29.969085823641983	39.29927866921831	15.66023848078905
25	16.540556455174443	33.34314735757397	33.990872957456205	16.125423229795377
26	16.443397615192108	34.980126600912705	31.105549830708078	17.470925953187106
27	16.20491682614456	35.32459885175916	32.509936699543644	15.960547622552628
28	14.700426910054468	33.16649492124245	36.222582069777715	15.910496098925364
29	15.43942293537465	28.959222729280143	36.661268953334314	18.940085382010892
30	16.013543353452082	33.2224348594141	33.9938171647284	16.77020462240542
31	20.088326218165758	31.876932136022372	32.0153098778154	16.019431767996466
32	19.199175621963786	31.349919034300015	33.59929339025467	15.851611953481527
33	19.107905196525834	31.847490063300455	31.971146768732517	17.07345797144119
34	17.088179007802147	29.807154423671427	33.513911379361105	19.590755189165318
35	18.875312822022668	29.118209921978504	33.91432356837921	18.092153687619607
36	20.58884145443839	31.803326954217574	31.22331812159576	16.38451346974827
37	16.243191520683055	34.423671426468424	32.957456204916824	16.375680847931694
38	16.696599440600618	33.67878698660386	31.826880612395115	17.79773296040041
39	17.52392168408656	32.84263212130134	32.062417194170465	17.571029000441634
40	18.928308552922125	31.102605623435892	31.820992197850728	18.148093625791255
41	16.62593846606801	30.390107463565435	32.77491535404092	20.20903871632563
42	16.360959811570737	30.210510819961726	32.10363609598116	21.324893272486385
43	17.74768143677315	30.764021787133817	32.383335786839396	19.104960989253644
44	16.06948329162373	32.816134255851615	31.92992786692183	19.184454585602825
45	16.02237597526866	35.43647872810246	30.54615044899161	17.994994847637276
46	16.56116590607979	35.710290004416315	30.351832769026938	17.37671132047696
47	17.488591196820256	30.77285440895039	32.792580597674075	18.945973796555275
48	17.161784189606948	30.884734285293685	34.79464154276461	17.158839982334754
49	17.126453702340644	31.29986751067275	33.30487266303548	18.268806123951126
50	16.46400706609745	33.1517738848815	32.75136169586339	17.632857353157664
51	17.347269247755044	30.616811423524215	32.221404386868834	19.814514941851908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	30.0
1	45.5
2	61.0
3	365.0
4	669.0
5	474.0
6	279.0
7	259.5
8	240.0
9	255.0
10	270.0
11	262.0
12	254.0
13	232.5
14	211.0
15	217.5
16	224.0
17	210.0
18	196.0
19	204.5
20	213.0
21	268.0
22	323.0
23	370.5
24	418.0
25	540.5
26	801.5
27	940.0
28	1149.0
29	1358.0
30	1538.0
31	1718.0
32	1913.5
33	2109.0
34	2205.5
35	2302.0
36	2424.5
37	2547.0
38	2626.5
39	2706.0
40	2817.0
41	2928.0
42	2926.5
43	2925.0
44	2716.5
45	2508.0
46	2403.0
47	2298.0
48	2036.5
49	1775.0
50	1504.0
51	1233.0
52	995.5
53	758.0
54	618.5
55	479.0
56	409.5
57	340.0
58	321.5
59	303.0
60	288.0
61	273.0
62	227.5
63	182.0
64	141.5
65	101.0
66	75.5
67	50.0
68	41.5
69	33.0
70	23.0
71	13.0
72	13.5
73	14.0
74	10.0
75	6.5
76	7.0
77	5.0
78	3.0
79	3.5
80	4.0
81	2.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	33965.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.14323568379214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.92916930173729	66.42426026792286
2	11.528588966184294	18.248196673045783
3	2.998400357129571	7.119093184160165
4	0.9225847252706372	2.9206536140144266
5	0.2901677764964101	1.1482408361548653
6	0.12648338975484544	0.6006182835271603
7	0.05208139578140694	0.28853231267481233
8	0.03348089728804732	0.21198292359782128
9	0.01860049849335962	0.1324893272486383
>10	0.08928239276812619	1.5398204033563963
>50	0.0037200996986719247	0.17370822905932579
>100	0.0074401993973438494	1.1924039452377446
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	280	0.8243780362137495	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	125	0.3680259090239953	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	59	0.17370822905932579	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	41	0.12071249815987047	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	37	0.1089356690711026	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	37	0.1089356690711026	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05888414544383925	0.0
2	0.0	0.0	0.0	0.13837774179302223	0.0
3	0.0	0.0	0.0	0.19726188723686147	0.0
4	0.0	0.0	0.0	0.2738112763138525	0.0
5	0.0	0.0	0.0	0.471073163550714	0.0
6	0.0	0.0	0.0	0.6035624907993523	0.0
7	0.0	0.0	0.0	0.7242749889592227	0.0
8	0.0	0.0	0.0	0.939202119829236	0.0
9	0.0	0.0	0.0	1.0216399234506108	0.0
10	0.0	0.0	0.0	1.2954511997644633	0.0
11	0.0	0.0	0.0	1.5162667451788605	0.0
12	0.0	0.0	0.0	1.869571617841896	0.0
13	0.0	0.0	0.0	1.9991167378183423	0.0
14	0.0	0.0	0.0	2.04033563962903	0.0
15	0.0	0.0	0.0	2.108052406889445	0.0
16	0.0	0.0	0.0	2.208155454143972	0.0
17	0.0	0.0	0.0	2.3700868541145295	0.0
18	0.0	0.0	0.0	2.511408803179744	0.0
19	0.0	0.0	0.0	2.643898130428382	0.0
20	0.0	0.0	0.0	2.755778006771677	0.0
21	0.0	0.0	0.0	2.900044163109083	0.0
22	0.0	0.0	0.0	3.035477697629913	0.0
23	0.0	0.0	0.0	3.1797438539673193	0.0
24	0.0	0.0	0.0	3.27101427940527	0.0
25	0.0	0.0	0.0	3.365228912115413	0.0
26	0.0	0.0	0.0	3.430001472103636	0.0
27	0.0	0.0	0.0	3.5507139702635064	0.0
28	0.0	0.0	0.0	3.6979243338731047	0.0
29	0.0	0.0	0.0	3.8304136611217428	0.0
30	0.0	0.0	0.0	4.0158987192698365	0.0
31	0.0	0.0	0.0	4.17194170469601	0.0
32	0.0	0.0	0.0	4.266156337406153	0.0
33	0.0	0.0	0.0	4.404534079199176	0.0
34	0.0	0.0	0.0	4.51641395554247	0.0
35	0.0	0.0	0.0	4.669512733696452	0.0
36	0.0	0.0	0.0	4.8461651700279695	0.0
37	0.0	0.0	0.0	5.01104077727072	0.0
38	0.0	0.0	0.0	5.214191079051965	0.0
39	0.0	0.0	0.0	5.411452966288826	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGAT	20	6.9368887E-4	45.0	5
GGATGGC	25	3.8111324E-5	45.0	8
GGATGGA	20	6.9368887E-4	45.0	8
GGATGAC	25	3.8111324E-5	45.0	8
TACGGGA	20	6.9368887E-4	45.0	4
GCAGGGA	40	6.4846972E-9	45.0	4
GATTGGG	20	6.9368887E-4	45.0	3
ATAATGG	20	6.9368887E-4	45.0	2
CGAGGGA	20	6.9368887E-4	45.0	4
GATCTTT	20	6.9368887E-4	45.0	9
AGCGGGA	20	6.9368887E-4	45.0	4
CGGGATG	20	6.9368887E-4	45.0	6
CGGGACA	25	3.8111324E-5	45.0	6
TGTAGGG	50	2.0008883E-11	45.0	3
ACGGGAG	25	3.8111324E-5	45.0	5
CTAAAGC	20	6.9368887E-4	45.0	5
AAGGATG	40	6.4846972E-9	45.0	6
GTAGGGC	35	1.1660268E-7	45.0	4
ACTAGGA	20	6.9368887E-4	45.0	4
ACCTATG	25	3.8111324E-5	45.0	34
>>END_MODULE