##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549359_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	77362
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89497427677671	31.0	31.0	34.0	30.0	34.0
2	32.20502313797471	33.0	31.0	34.0	30.0	34.0
3	32.045565006075336	33.0	31.0	34.0	30.0	34.0
4	35.85991830614513	37.0	35.0	37.0	35.0	37.0
5	32.201675241074426	37.0	35.0	37.0	0.0	37.0
6	34.0313332126884	37.0	35.0	37.0	19.0	37.0
7	35.47116155218324	37.0	35.0	37.0	32.0	37.0
8	35.68916263798764	37.0	35.0	37.0	35.0	37.0
9	37.90650448540627	39.0	38.0	39.0	35.0	39.0
10	37.55302344820454	39.0	37.0	39.0	35.0	39.0
11	37.354747809001836	39.0	37.0	39.0	34.0	39.0
12	37.31636979395569	39.0	37.0	39.0	34.0	39.0
13	37.3264910421137	39.0	37.0	39.0	34.0	39.0
14	38.65890230345648	40.0	38.0	41.0	34.0	41.0
15	38.72221504097619	40.0	38.0	41.0	35.0	41.0
16	38.709379281817945	40.0	38.0	41.0	35.0	41.0
17	38.58480907939298	40.0	38.0	41.0	34.0	41.0
18	38.45824823556785	40.0	38.0	41.0	34.0	41.0
19	38.31723585222719	40.0	37.0	41.0	34.0	41.0
20	38.225110519376436	40.0	36.0	41.0	34.0	41.0
21	38.1662961143714	40.0	36.0	41.0	34.0	41.0
22	38.013171841472555	40.0	35.0	41.0	34.0	41.0
23	37.96654688348285	40.0	35.0	41.0	34.0	41.0
24	37.93429590755151	40.0	35.0	41.0	34.0	41.0
25	37.80489129029756	40.0	35.0	41.0	34.0	41.0
26	37.72990615547685	40.0	35.0	41.0	34.0	41.0
27	36.73165119826271	40.0	35.0	41.0	32.0	41.0
28	37.01574416380135	39.0	35.0	41.0	32.0	41.0
29	37.24126832294925	39.0	35.0	41.0	32.0	41.0
30	37.17082029937178	39.0	35.0	41.0	33.0	41.0
31	37.059758020733696	39.0	35.0	40.0	33.0	41.0
32	36.81702903234146	39.0	35.0	40.0	32.0	41.0
33	36.49934076161423	39.0	35.0	40.0	31.0	41.0
34	36.163116258628264	39.0	35.0	40.0	30.0	41.0
35	35.92585507096507	39.0	35.0	40.0	29.0	41.0
36	35.79655386365399	39.0	35.0	40.0	27.0	41.0
37	35.67911894728678	39.0	35.0	40.0	27.0	41.0
38	35.53119102401696	38.0	35.0	40.0	27.0	41.0
39	35.43419249760864	38.0	35.0	40.0	26.0	41.0
40	35.31830873038442	38.0	35.0	40.0	25.0	41.0
41	35.044750652775264	38.0	35.0	40.0	24.0	41.0
42	35.018562084744445	38.0	35.0	40.0	24.0	41.0
43	34.889415992347665	38.0	35.0	40.0	24.0	41.0
44	34.74733073084977	38.0	34.0	40.0	23.0	41.0
45	34.66519738372845	38.0	34.0	40.0	23.0	41.0
46	34.55226079987591	38.0	34.0	40.0	23.0	41.0
47	34.32954163542825	37.0	34.0	40.0	23.0	40.0
48	34.24381479279233	37.0	34.0	40.0	23.0	40.0
49	34.12269589721052	37.0	34.0	40.0	23.0	40.0
50	34.042617822703654	37.0	34.0	39.0	23.0	40.0
51	34.27697060572374	38.0	34.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	3.0
17	13.0
18	15.0
19	50.0
20	116.0
21	156.0
22	242.0
23	401.0
24	527.0
25	865.0
26	1274.0
27	1488.0
28	1251.0
29	1387.0
30	1460.0
31	1653.0
32	2004.0
33	2844.0
34	4431.0
35	6499.0
36	6083.0
37	9032.0
38	17563.0
39	18003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.85119309221582	24.47325560352628	29.864791499702697	19.810759804555207
2	32.01701093560146	26.282929603681392	29.58299940539283	12.11706005532432
3	24.77960755926682	25.67927406220108	37.608903596080765	11.932214782451332
4	22.460639590496626	26.047671983661232	38.692122747602184	12.799565678239963
5	20.02275018743052	36.9974923088855	31.237558491248933	11.742199012435044
6	19.87668364313229	38.106563946123416	31.755900829864792	10.260851580879502
7	81.97047646131176	3.2328533388485305	13.180889842558363	1.6157803572813527
8	82.77448876709495	3.6193480003102296	11.840438458157752	1.765724774437062
9	78.13655282955456	5.836198650500245	12.618598278224452	3.4086502417207414
10	32.50820816421499	42.48597502649879	17.45559835578191	7.5502184535043035
11	22.55887903621933	24.7989969235542	38.01090974897237	14.631214291254105
12	23.985936247770223	22.283550063338588	37.019466921744524	16.711046767146662
13	20.24637418887826	25.624983842196425	37.21982368604741	16.9088182828779
14	14.847082546986892	29.263721206794035	37.360719733202345	18.528476513016727
15	14.48773299552752	27.90905095524935	41.26056720353662	16.342648845686515
16	17.282386701481347	27.854760735244692	38.55381194901889	16.30904061425506
17	16.880380548589745	27.70093844523151	36.56834104599157	18.85033996018717
18	16.844187068586645	27.900002585248572	38.61456529045268	16.641245055712105
19	19.035185233060158	28.512706496729663	35.35198159303017	17.10012667718001
20	20.97541428608361	27.659574468085108	37.53780926036038	13.827201985470902
21	19.6711563816861	28.590263953879163	37.363304981774	14.375274682660738
22	19.267857604508674	23.83469920632869	38.124660686124976	18.772782503037668
23	16.9346707685944	28.20506191670329	38.15309842041312	16.707168894289186
24	16.13324371138285	27.048163180889844	40.68534939634446	16.13324371138285
25	15.41583723274993	31.874822264160702	36.351180165972956	16.358160337116416
26	15.67823996277242	32.250975931335795	35.434709547322974	16.636074558568804
27	15.128874641296761	34.301078048654375	35.02753289728807	15.542514412760786
28	13.92285618262196	30.935084408365864	39.68873607197332	15.453323337038857
29	16.539127737131924	26.372120679403327	38.94806235619555	18.140689227269203
30	16.894599415733826	30.242237791163625	36.85142576458726	16.01173702851529
31	18.21178356298958	29.74069956826349	35.45022103875288	16.597295829994057
32	19.799126185982782	29.05173082391872	35.29639874873969	15.852744241358806
33	18.342338615857916	29.38910576251907	34.890514722990616	17.378040898632406
34	17.551252552932965	29.06724231534862	35.56914247304878	17.81236265866963
35	17.45042785863861	29.823427522556294	34.77030066440888	17.955843954396215
36	19.70476461311755	29.97724981256948	33.90294976862025	16.415035805692717
37	17.14278327861224	31.665417129857037	35.57431297019208	15.617486621338642
38	17.072981567177685	31.232387994105633	33.769809467180266	17.924820971536413
39	17.604250148651793	31.24919210982136	33.81117344432667	17.335384297200175
40	18.26995165585171	30.432253561179905	33.552648587161656	17.745146195806726
41	16.311625862826713	30.224141051162068	34.164059874356916	19.3001732116543
42	16.9243297743078	30.003102298285985	33.91846126005015	19.154106667356068
43	17.456890980067733	30.14529096972674	33.150642434270054	19.247175615935472
44	16.48483751712727	31.35906517411649	33.365218065717016	18.790879243039218
45	16.381427574261266	32.57283937850624	32.74863628137846	18.297096765854036
46	17.586153408650244	32.95287091853882	32.385408857061606	17.075566815749337
47	16.594710581422405	30.365037098317003	34.75091130012151	18.289341020139087
48	16.87391742716062	29.515782942529924	35.308032367312116	18.302267262997336
49	16.63995243142628	30.6946562912024	34.863369612988286	17.80202166438303
50	16.74853287143559	30.99713037408547	34.388976500090486	17.86536025438846
51	16.06990512137742	29.50802719681497	34.25712882293633	20.16493885887128
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	97.5
2	139.0
3	1335.5
4	2532.0
5	1676.5
6	821.0
7	775.0
8	729.0
9	771.0
10	813.0
11	796.0
12	779.0
13	797.5
14	816.0
15	797.0
16	778.0
17	765.0
18	752.0
19	720.0
20	688.0
21	755.0
22	822.0
23	956.5
24	1091.0
25	1243.5
26	1545.5
27	1695.0
28	2064.0
29	2433.0
30	2727.0
31	3021.0
32	3464.5
33	3908.0
34	4396.0
35	4884.0
36	4945.0
37	5006.0
38	5187.0
39	5368.0
40	5460.0
41	5552.0
42	5785.0
43	6018.0
44	5888.5
45	5759.0
46	6035.0
47	6311.0
48	5326.0
49	4341.0
50	3852.5
51	3364.0
52	2776.5
53	2189.0
54	1814.5
55	1440.0
56	1257.0
57	1074.0
58	990.0
59	906.0
60	834.5
61	763.0
62	649.5
63	536.0
64	407.0
65	278.0
66	204.0
67	130.0
68	101.0
69	72.0
70	54.5
71	37.0
72	35.0
73	33.0
74	25.5
75	13.5
76	9.0
77	5.5
78	2.0
79	2.5
80	3.0
81	1.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	77362.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.67849848762959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91758369317918	41.7129857035754
2	10.563216789710731	11.762881001008246
3	4.738357245670242	7.914738502106977
4	2.8462645679528253	6.3390294976862025
5	1.9617402609462784	5.461337607610972
6	1.3906300784696104	4.645691683255345
7	1.0377489901100432	4.0446213903466814
8	0.7684450016251103	3.422869108864817
9	0.5270000464317222	2.6408314159406427
>10	1.2257974648279704	8.604999870737572
>50	0.011607930538143659	0.4562963728962539
>100	0.009286344430514928	1.0638297872340425
>500	0.0	0.0
>1k	0.002321586107628732	1.9298880587368474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1493	1.9298880587368474	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCG	293	0.37873891574674906	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	261	0.3373749386003464	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGC	149	0.19260101858793724	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120	0.15511491429900984	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	91	0.11762881001008248	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCT	83	0.1072878157234818	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028437734288151807	0.0
2	0.0	0.0	0.0	0.1796747757296864	0.0
3	0.0	0.0	0.0	0.25593960859336623	0.0
4	0.0	0.0	0.0	0.4472480028954784	0.0
5	0.0	0.0	0.0	1.02246581008764	0.0
6	0.0	0.0	0.0	1.3650112458312866	0.0
7	0.0	0.0	0.0	1.6493885887128048	0.0
8	0.0	0.0	0.0	2.079832475892557	0.0
9	0.0	0.0	0.0	2.233654765905742	0.0
10	0.0	0.0	0.0	2.9368423773945866	0.0
11	0.0	0.0	0.0	3.350482148858613	0.0
12	0.0	0.0	0.0	3.9166515860499986	0.0
13	0.0	0.0	0.0	4.066596003205708	0.0
14	0.0	0.0	0.0	4.127349344639487	0.0
15	0.0	0.0	0.0	4.2126625475039425	0.0
16	0.0	0.0	0.0	4.3548512189447015	0.0
17	0.0	0.0	0.0	4.534525994674388	0.0
18	0.0	0.0	0.0	4.724541764690675	0.0
19	0.0	0.0	0.0	4.922313280421912	0.0
20	0.0	0.0	0.0	5.073550321863447	0.0
21	0.0	0.0	0.0	5.254517721878958	0.0
22	0.0	0.0	0.0	5.497531087614074	0.0
23	0.0	0.0	0.0	5.697887851916962	0.0
24	0.0	0.0	0.0	5.8400765233577205	0.0
25	0.0	0.0	0.0	6.000361934800031	0.0
26	0.0	0.0	0.0	6.138672733383315	0.0
27	0.0	0.0	0.0	6.309299139112226	0.0
28	0.0	0.0	0.0	6.466999301982885	0.0
29	0.0	0.0	0.0	6.633747834854321	0.0
30	0.0	0.0	0.0	6.850908714872935	0.0
31	0.0	0.0	0.0	7.035753987745922	0.0
32	0.0	0.0	0.0	7.193454150616581	0.0
33	0.0	0.0	0.0	7.371836302060443	0.0
34	0.0	0.0	0.0	7.542462707789354	0.0
35	0.0	0.0	0.0	7.737648974948941	0.0
36	0.0	0.0	0.0	7.928957369251053	0.0
37	0.0	0.0	0.0	8.13448463069724	0.0
38	0.0	0.0	0.0	8.320622527856054	0.0
39	0.0	0.0	0.0	8.575269512163594	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	6.9925125E-4	45.0	1
ATGGGCG	20	6.9925125E-4	45.0	5
GCGACCA	35	1.192384E-7	45.0	11
GGGTCTC	40	6.673872E-9	45.0	8
GTGGGAC	20	6.9925125E-4	45.0	5
GCAAAGG	20	6.9925125E-4	45.0	2
TAAACCT	20	6.9925125E-4	45.0	29
ACACACC	20	6.9925125E-4	45.0	28
GCACGGG	20	6.9925125E-4	45.0	3
GGATCCA	35	1.192384E-7	45.0	8
TGTGCGG	20	6.9925125E-4	45.0	2
ACGGGGC	20	6.9925125E-4	45.0	5
CGTTATT	75	0.0	45.0	1
AACATAC	20	6.9925125E-4	45.0	16
CTACGGG	20	6.9925125E-4	45.0	3
GATATCT	20	6.9925125E-4	45.0	9
GGTATAC	20	6.9925125E-4	45.0	9
CGGGAGG	40	6.673872E-9	45.0	6
CATACGA	20	6.9925125E-4	45.0	18
TGGGCGA	40	6.673872E-9	45.0	6
>>END_MODULE