FastQCFastQC Report
Sat 18 Jun 2016
SRR3549350_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549350_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825553
Sequences flagged as poor quality0
Sequence length51
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT18170.22009489396804324No Hit
AAGGAAAGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18130.2196103702609039No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17530.21234251465381387No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16660.20180412402353332No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13510.16364788208631062No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA12870.15589550277208125No Hit
GCACAAAAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA8650.10477825166888134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG302.1647556E-645.0000042
ACGTTAG351.2113742E-745.0000042
CGTTGAT302.1647556E-645.00000425
TACGGTA406.8102963E-945.0000041
CGACACT207.032129E-445.00000431
TGTGCGC207.032129E-445.00000432
TCGGCGA302.1647556E-645.00000411
ATCCGAA302.1647556E-645.0000041
CCGTAAG207.032129E-445.00000435
TACGCAA302.1647556E-645.0000041
TGCGATA207.032129E-445.0000041
TGCGAAG1200.045.0000042
CGGGCAC207.032129E-445.0000046
TCATGCG207.032129E-445.00000414
GTCGTTT406.8102963E-945.0000049
TCCGTAA207.032129E-445.00000416
TCGTACT207.032129E-445.00000426
CGAGCCG207.032129E-445.00000430
CCGAATC302.1647556E-645.00000414
ATCGGCG302.1647556E-645.00000410