##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549350_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	825553
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65084373747052	31.0	31.0	33.0	30.0	34.0
2	32.00273392501753	31.0	31.0	34.0	30.0	34.0
3	32.020191314185766	33.0	31.0	34.0	30.0	34.0
4	35.80313801778929	37.0	35.0	37.0	35.0	37.0
5	35.69786191801132	37.0	35.0	37.0	35.0	37.0
6	35.858396735279264	37.0	35.0	37.0	35.0	37.0
7	4.66491188330731	0.0	0.0	0.0	0.0	35.0
8	19.493296008857094	17.0	17.0	17.0	17.0	35.0
9	31.642692837407168	32.0	32.0	32.0	28.0	37.0
10	35.047168382890014	37.0	34.0	37.0	32.0	37.0
11	36.82560780470787	37.0	37.0	39.0	34.0	39.0
12	37.23852859840616	39.0	37.0	39.0	34.0	39.0
13	37.130183040943464	39.0	37.0	39.0	34.0	39.0
14	38.573252111009225	40.0	38.0	41.0	35.0	41.0
15	38.70247942894036	40.0	38.0	41.0	35.0	41.0
16	38.72357437983994	40.0	38.0	41.0	35.0	41.0
17	38.654889510425136	40.0	38.0	41.0	35.0	41.0
18	38.54399293564435	40.0	38.0	41.0	34.0	41.0
19	38.517631212048165	40.0	38.0	41.0	34.0	41.0
20	38.4541404367739	40.0	38.0	41.0	34.0	41.0
21	38.36687287188103	40.0	38.0	41.0	34.0	41.0
22	38.28794759391584	40.0	37.0	40.0	34.0	41.0
23	38.18496329127264	40.0	37.0	40.0	34.0	41.0
24	38.0880500706799	40.0	37.0	40.0	34.0	41.0
25	37.949848162383276	39.0	37.0	40.0	33.0	41.0
26	37.72314921028692	39.0	37.0	40.0	33.0	41.0
27	37.541192388617084	39.0	36.0	40.0	33.0	41.0
28	37.36238739366219	39.0	36.0	40.0	33.0	41.0
29	37.275041093666914	39.0	36.0	40.0	33.0	41.0
30	37.029754600855426	38.0	36.0	40.0	32.0	41.0
31	36.872594491207714	38.0	36.0	40.0	32.0	41.0
32	36.583359275540154	38.0	35.0	40.0	31.0	40.0
33	36.413169112098196	38.0	35.0	40.0	31.0	40.0
34	36.16503604250727	38.0	35.0	40.0	31.0	40.0
35	35.894892272210264	38.0	35.0	40.0	30.0	40.0
36	35.74122921241883	38.0	35.0	40.0	30.0	40.0
37	35.61946234826837	38.0	35.0	39.0	30.0	40.0
38	35.34766877474856	38.0	34.0	39.0	30.0	40.0
39	35.120125540092516	37.0	34.0	39.0	29.0	40.0
40	34.8318496813651	37.0	34.0	39.0	29.0	40.0
41	34.39331575319816	36.0	33.0	38.0	27.0	40.0
42	34.20773832812672	36.0	33.0	38.0	27.0	40.0
43	34.1073583404094	36.0	33.0	38.0	26.0	40.0
44	33.7502571003921	36.0	33.0	38.0	25.0	40.0
45	33.48845803964131	36.0	32.0	38.0	24.0	39.0
46	33.29574600298224	35.0	32.0	38.0	24.0	39.0
47	33.11553952320445	35.0	31.0	38.0	24.0	39.0
48	32.805584862510344	35.0	31.0	38.0	24.0	39.0
49	32.5523509695925	35.0	31.0	38.0	24.0	39.0
50	32.39706960061922	34.0	31.0	37.0	24.0	39.0
51	32.22324066413665	34.0	30.0	37.0	23.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	5.0
16	24.0
17	56.0
18	157.0
19	331.0
20	654.0
21	1373.0
22	2386.0
23	3821.0
24	5367.0
25	6815.0
26	7890.0
27	9149.0
28	11372.0
29	14639.0
30	19759.0
31	27529.0
32	39482.0
33	56883.0
34	83777.0
35	146380.0
36	245400.0
37	114973.0
38	24716.0
39	2611.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.791973380267528	26.174697445227622	31.72455311772836	13.30877605677649
2	27.351605529869072	28.373829421006285	32.38180952646287	11.89275552266178
3	29.389754503950684	26.707673523080892	31.24681274248897	12.655759230479449
4	27.663638797266803	28.401205010459655	30.165476959080763	13.76967923319278
5	23.187608790713618	34.74216676579214	27.52700311185351	14.54322133164073
6	21.85323050125189	44.476490304074964	24.82251290952852	8.847766285144624
7	11.6737508070348	87.46694639835358	0.6499885531274188	0.209314241484193
8	89.2583516745745	4.677712999649931	4.388694608341318	1.675240717434253
9	80.86446297209264	10.315267463142886	6.062239492800583	2.7580300719638835
10	25.32447947012487	53.95280496830609	11.646496348508212	9.07621921306082
11	30.430026903178835	26.17639327820261	28.60797550248137	14.785604316137183
12	31.915213196487684	24.86854266170676	27.039330000617767	16.176914141187783
13	22.619141351312393	31.867851004114815	27.096382667133422	18.416624977439362
14	16.162136168120036	33.48192060352273	29.27758726574793	21.078355962609304
15	16.33341529859379	32.41596844781619	34.32099453336128	16.929621720228745
16	20.30239124562566	30.69215422874122	32.17455451073402	16.830900014899104
17	18.215184246196188	31.96984324446765	28.856415033317063	20.958557476019106
18	18.663126413446502	32.42783927864111	31.951067950815997	16.957966357096392
19	23.10693559347492	30.603728652188288	28.25100266124646	18.038333093090326
20	22.74063567087758	30.92339316797347	31.090553846936537	15.245417314212412
21	20.73434413054038	32.14826909962171	31.01242439916032	16.104962370677594
22	21.91113108425504	27.893303034450845	29.703483604323406	20.49208227697071
23	19.025792408240292	31.26776839282275	31.35316569620606	18.353273502730897
24	15.791838924938798	30.42518166610745	37.63986079633894	16.143118612614817
25	16.79443960593687	34.61098197208416	31.205991620162486	17.38858680181648
26	17.099083886800727	37.16151476646563	27.55062364257655	18.18877770415709
27	17.52485909444942	36.179385212094196	29.00819208457846	17.287563608877928
28	15.264677131571203	34.49481741329751	33.39567538365193	16.84483007147936
29	15.79813773313161	29.624748501913263	34.261155855529566	20.31595790942556
30	16.611653037418556	34.7848048520204	31.02792915778878	17.575612952772264
31	21.547980565754106	31.60729838060064	29.73824818031065	17.106472873334603
32	20.219779953558405	31.782574831658295	30.80214111026185	17.195504104521454
33	20.423643303337276	32.106478929880936	29.29672595217993	18.173151814601848
34	18.201617582396285	30.310712940295776	30.911764599002122	20.575904878305813
35	20.22292935765481	28.410168699041733	32.38835059650925	18.97855134679421
36	21.915370666692507	31.30265409973678	29.53644405628712	17.245531177283592
37	16.84955417762397	33.8990955153697	32.04421763351353	17.207132673492797
38	16.88213839692909	33.89449254015187	31.452493056169622	17.770876006749415
39	18.81696269046324	32.46030236701944	31.144578240282573	17.578156702234747
40	19.99665678642074	31.46727102923737	30.71795511614639	17.818117068195498
41	17.37029603187197	30.478600404819556	31.506275187662087	20.644828375646384
42	16.671491715250262	29.65006486561129	31.790084949118953	21.88835847001949
43	18.73653175507811	29.172203359445128	32.268430978992264	19.8228339064845
44	16.3557033891222	32.73744992750314	30.819826225572434	20.087020457802225
45	16.237237342726633	35.84082427173059	28.895661453595345	19.026276931947432
46	16.678032785296644	36.116397130166085	29.287641132671073	17.917928951866205
47	18.739075504540594	30.474360822382092	31.39229098555756	19.39427268751976
48	17.67905876424651	30.50040397164083	33.92901485428555	17.89152240982711
49	18.43649044943208	30.85592324175431	31.692211160276806	19.015375148536798
50	17.279205574929772	32.99352070672628	31.624983495911223	18.10229022243272
51	18.249706560329866	30.253539142853334	31.244874647660414	20.251879649156386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	463.0
1	774.5
2	1086.0
3	2404.0
4	3722.0
5	3623.0
6	3524.0
7	3082.5
8	2641.0
9	2689.0
10	2737.0
11	2729.5
12	2722.0
13	2753.5
14	2785.0
15	3062.0
16	3339.0
17	3217.0
18	3095.0
19	4403.0
20	5711.0
21	6325.5
22	6940.0
23	9090.5
24	11241.0
25	14372.0
26	23311.5
27	29120.0
28	34737.0
29	40354.0
30	43422.0
31	46490.0
32	50999.5
33	55509.0
34	58824.0
35	62139.0
36	63311.5
37	64484.0
38	68877.5
39	73271.0
40	75580.0
41	77889.0
42	74230.0
43	70571.0
44	66414.0
45	62257.0
46	56453.0
47	50649.0
48	44378.0
49	38107.0
50	32086.0
51	26065.0
52	21041.0
53	16017.0
54	13942.0
55	11867.0
56	10646.5
57	9426.0
58	8721.5
59	8017.0
60	6983.0
61	5949.0
62	4796.5
63	3644.0
64	3072.5
65	2501.0
66	1989.0
67	1477.0
68	1208.0
69	939.0
70	742.5
71	546.0
72	422.5
73	299.0
74	243.5
75	154.5
76	121.0
77	86.0
78	51.0
79	47.0
80	43.0
81	39.0
82	35.0
83	23.0
84	11.0
85	9.0
86	7.0
87	4.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	825553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.822663790529013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67328636662172	16.2957002251268
2	7.814889997885252	3.410834339676357
3	3.1920839637030096	2.0897932539308997
4	1.9255394383888342	1.6808159911745435
5	1.3580734083095434	1.4818389696198497
6	1.0172441829651317	1.331938667863167
7	0.9291378270032217	1.4193383689627812
8	0.7770220065545184	1.3565352005505187
9	0.6546986751864168	1.285854216542814
>10	5.552943262261735	24.54150581261511
>50	1.3436805880478766	21.37260150464059
>100	0.7552237321037986	22.098868040118163
>500	0.0028075231676721133	0.4473762647256202
>1k	0.0033690278012065357	1.1869991444527752
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1817	0.22009489396804324	No Hit
AAGGAAAGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1813	0.2196103702609039	No Hit
AAGGAAAGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1753	0.21234251465381387	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1666	0.20180412402353332	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1351	0.16364788208631062	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1287	0.15589550277208125	No Hit
GCACAAAAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	865	0.10477825166888134	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0024226185356966785	0.0
2	0.0	0.0	0.0	0.013687794726686233	0.0
3	0.0	0.0	0.0	0.020228864773067266	0.0
4	0.0	0.0	0.0	0.028586898721220805	0.0
5	0.0	0.0	0.0	0.05572022632102361	0.0
6	0.0	0.0	0.0	0.07582796016730603	0.0
7	0.0	0.0	0.0	0.09339194455110696	0.0
8	0.0	0.0	0.0	0.38664991829718987	0.0
9	0.0	0.0	0.0	0.49360852664819826	0.0
10	0.0	0.0	0.0	0.7575528161123514	0.0
11	0.0	0.0	0.0	1.0206491890890106	0.0
12	0.0	0.0	0.0	1.2784158012871372	0.0
13	0.0	0.0	0.0	1.387918159100627	0.0
14	1.2113092678483392E-4	0.0	0.0	1.4286181505003313	0.0
15	1.2113092678483392E-4	0.0	0.0	1.4939077200373567	0.0
16	1.2113092678483392E-4	0.0	0.0	1.6217008477953565	0.0
17	1.2113092678483392E-4	0.0	0.0	1.775415993885311	0.0
18	1.2113092678483392E-4	0.0	0.0	1.9486332191876232	0.0
19	1.2113092678483392E-4	0.0	0.0	2.048202841004757	0.0
20	1.2113092678483392E-4	0.0	0.0	2.1808412058341498	0.0
21	1.2113092678483392E-4	0.0	0.0	2.338917065288358	0.0
22	1.2113092678483392E-4	0.0	0.0	2.5158893493210006	0.0
23	1.2113092678483392E-4	0.0	0.0	2.683413421064426	0.0
24	1.2113092678483392E-4	0.0	0.0	2.8150827384795405	0.0
25	1.2113092678483392E-4	0.0	0.0	2.939484200287565	0.0
26	1.2113092678483392E-4	0.0	0.0	3.05964607965812	0.0
27	1.2113092678483392E-4	0.0	0.0	3.1825939703447266	0.0
28	1.2113092678483392E-4	0.0	0.0	3.315595727954474	0.0
29	1.2113092678483392E-4	0.0	0.0	3.479122479114	0.0
30	1.2113092678483392E-4	0.0	0.0	3.656458155926997	0.0
31	1.2113092678483392E-4	0.0	0.0	3.816956633916902	0.0
32	1.2113092678483392E-4	0.0	0.0	3.9661899357158172	0.0
33	1.2113092678483392E-4	0.0	0.0	4.109972345809415	0.0
34	1.2113092678483392E-4	0.0	0.0	4.269865169165396	0.0
35	1.2113092678483392E-4	0.0	0.0	4.457496974755103	0.0
36	1.2113092678483392E-4	0.0	0.0	4.640162412346633	0.0
37	1.2113092678483392E-4	0.0	0.0	4.819315053061403	0.0
38	1.2113092678483392E-4	0.0	0.0	5.00294953806721	0.0
39	1.2113092678483392E-4	0.0	0.0	5.1931250931194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	30	2.1647556E-6	45.000004	2
ACGTTAG	35	1.2113742E-7	45.000004	2
CGTTGAT	30	2.1647556E-6	45.000004	25
TACGGTA	40	6.8102963E-9	45.000004	1
CGACACT	20	7.032129E-4	45.000004	31
TGTGCGC	20	7.032129E-4	45.000004	32
TCGGCGA	30	2.1647556E-6	45.000004	11
ATCCGAA	30	2.1647556E-6	45.000004	1
CCGTAAG	20	7.032129E-4	45.000004	35
TACGCAA	30	2.1647556E-6	45.000004	1
TGCGATA	20	7.032129E-4	45.000004	1
TGCGAAG	120	0.0	45.000004	2
CGGGCAC	20	7.032129E-4	45.000004	6
TCATGCG	20	7.032129E-4	45.000004	14
GTCGTTT	40	6.8102963E-9	45.000004	9
TCCGTAA	20	7.032129E-4	45.000004	16
TCGTACT	20	7.032129E-4	45.000004	26
CGAGCCG	20	7.032129E-4	45.000004	30
CCGAATC	30	2.1647556E-6	45.000004	14
ATCGGCG	30	2.1647556E-6	45.000004	10
>>END_MODULE