FastQCFastQC Report
Sat 18 Jun 2016
SRR3549344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875047
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17300.1977036662030725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT253.890039E-545.00000421
CGCTAAT253.890039E-545.00000434
TTACGTA253.890039E-545.0000041
CTAGGCG253.890039E-545.0000045
TGCGTAA502.1827873E-1145.0000041
GGTACCC207.0323626E-445.012
GATCGGT406.8121153E-945.010
GCGCGAA551.8189894E-1245.01
TACGCTA207.0323626E-445.01
TCGCTTA207.0323626E-445.01
TTGCGAA800.045.01
ACGCTAG453.8380676E-1045.02
GATTCGA207.0323626E-445.09
TATACGA207.0323626E-445.01
TACCGGT207.0323626E-445.039
GTCGAGA302.1649048E-644.9999961
CGCGAAG650.044.9999962
TGCGCAA650.044.9999961
AGGCGAT6250.044.6400037
AGGACCG3600.043.757