FastQCFastQC Report
Sat 18 Jun 2016
SRR3549340_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549340_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624329
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40880.654782975001962No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.16305505590802286No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8410.1347046188788283No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7570.12125017418700716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC406.8066583E-945.0000046
CGGGTCC302.1639025E-645.0000046
GGTACCG207.030807E-445.0000049
TCGCAAA207.030807E-445.0000041
CGAACGG207.030807E-445.0000042
AGTCACG207.030807E-445.0000041
GTCGAAG207.030807E-445.0000041
GCGAAAG406.8066583E-945.0000041
AACCCAT207.030807E-445.00000438
GACGTAT207.030807E-445.0000049
CAATTGC207.030807E-445.00000425
CACGAGC207.030807E-445.00000436
ACGGGTC207.030807E-445.0000045
ACGGGCC302.1639025E-645.0000045
CATTACG207.030807E-445.00000438
TATTGCG207.030807E-445.0000041
CCGTATG302.1639025E-645.0000041
TAACCGA207.030807E-445.00000421
TCTACGG302.1639025E-645.0000042
CTAGTCC207.030807E-445.00000442