##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	624329
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861832463332632	31.0	31.0	34.0	30.0	34.0
2	32.10654798992198	33.0	31.0	34.0	30.0	34.0
3	31.87693988265802	33.0	31.0	34.0	30.0	34.0
4	35.70333269798456	37.0	35.0	37.0	33.0	37.0
5	28.363957144390216	35.0	32.0	37.0	0.0	37.0
6	31.973187213792727	37.0	32.0	37.0	17.0	37.0
7	34.97256734830514	37.0	33.0	37.0	32.0	37.0
8	35.63229002657253	37.0	35.0	37.0	35.0	37.0
9	37.7078095042838	39.0	38.0	39.0	35.0	39.0
10	37.33955014103141	39.0	37.0	39.0	34.0	39.0
11	37.16450621387121	39.0	37.0	39.0	34.0	39.0
12	36.297793310898584	37.0	35.0	39.0	33.0	39.0
13	35.5076089689891	37.0	35.0	39.0	31.0	39.0
14	36.78358525713206	38.0	35.0	41.0	32.0	41.0
15	37.138181952143825	39.0	35.0	41.0	33.0	41.0
16	37.28744780396234	38.0	35.0	41.0	33.0	41.0
17	37.27420638797813	38.0	35.0	41.0	33.0	41.0
18	37.25149560568226	38.0	35.0	41.0	33.0	41.0
19	37.24219762336845	38.0	35.0	41.0	33.0	41.0
20	37.13533089124484	38.0	35.0	41.0	33.0	41.0
21	36.99846074745847	38.0	35.0	41.0	33.0	41.0
22	36.85729959684718	38.0	35.0	40.0	33.0	41.0
23	36.913321341792546	38.0	35.0	40.0	33.0	41.0
24	36.836791179009786	38.0	35.0	40.0	33.0	41.0
25	36.73181287430185	37.0	35.0	40.0	33.0	41.0
26	36.60421989047441	37.0	35.0	40.0	33.0	41.0
27	36.45126367668329	37.0	35.0	40.0	32.0	41.0
28	36.53412063191042	37.0	35.0	40.0	32.0	41.0
29	36.533673752140295	37.0	35.0	40.0	32.0	41.0
30	36.33313685572831	37.0	35.0	40.0	32.0	41.0
31	36.0874955352066	37.0	35.0	40.0	31.0	41.0
32	35.8198930371647	37.0	35.0	40.0	31.0	41.0
33	35.59961654832628	36.0	35.0	40.0	30.0	41.0
34	35.352413551188555	36.0	35.0	40.0	30.0	41.0
35	35.13463894837498	36.0	34.0	40.0	29.0	41.0
36	34.971366058600516	36.0	34.0	40.0	27.0	41.0
37	34.828944354659164	36.0	34.0	40.0	27.0	41.0
38	34.72275995508778	36.0	34.0	40.0	27.0	41.0
39	34.666568427864156	36.0	34.0	40.0	27.0	41.0
40	34.479921643876864	36.0	34.0	40.0	25.0	41.0
41	34.34610117422064	35.0	34.0	40.0	24.0	41.0
42	34.26630991031972	35.0	34.0	40.0	24.0	41.0
43	34.13922787504665	35.0	33.0	40.0	24.0	41.0
44	34.015464602797564	35.0	34.0	40.0	23.0	40.0
45	33.86900976888788	35.0	34.0	39.0	23.0	40.0
46	33.79026282617018	35.0	34.0	39.0	23.0	40.0
47	33.71913366189941	35.0	33.0	39.0	23.0	40.0
48	33.66172322605549	35.0	33.0	39.0	23.0	40.0
49	33.662801183350446	36.0	33.0	39.0	23.0	40.0
50	33.469214148309625	35.0	33.0	39.0	23.0	40.0
51	33.577938875176386	35.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	1.0
14	4.0
15	13.0
16	28.0
17	93.0
18	218.0
19	452.0
20	882.0
21	1366.0
22	2227.0
23	3444.0
24	5169.0
25	7598.0
26	10506.0
27	11353.0
28	11841.0
29	12772.0
30	14736.0
31	18170.0
32	24547.0
33	42880.0
34	65472.0
35	96391.0
36	41553.0
37	65315.0
38	93247.0
39	94047.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.236187971406103	24.074326196604677	30.575065390202923	16.1144204417863
2	30.937694709039626	24.91602984964658	31.39114152954612	12.755133911767674
3	27.41230985586125	24.40988645409712	35.08678917686028	13.091014513181351
4	23.95499808594507	27.991491665452028	34.15891300900647	13.894597239596433
5	17.416458309641232	45.448313309168725	26.012567092030004	11.122661289160042
6	21.009595902160562	39.878173206754774	29.47996969546505	9.632261195619616
7	84.31147680149408	4.197466399926961	9.408661138598399	2.082395659980555
8	84.62397229665768	3.869113880662279	8.776142066122189	2.7307717565578407
9	80.98406449163822	5.612585671977436	10.324204065484704	3.079145770899638
10	48.20727533079514	28.457111554965408	14.834165960575273	8.501447153664174
11	44.44419528806126	18.12121493635567	25.98053269990662	11.454057075676447
12	42.34033658535804	19.433343637729468	26.24609781060947	11.980221966303024
13	19.559238798774363	41.84908918214595	25.915823227817388	12.675848791262299
14	13.28546327337029	43.296883534162276	28.916644909975346	14.501008282492084
15	11.885079821696573	25.332156603329338	49.902054846082756	12.880708728891339
16	13.533249296444664	20.789679800233532	49.60910033011441	16.067970573207397
17	13.300679609628899	22.08322855417576	30.938335396882096	33.67775643931324
18	15.637748686990353	24.297766081665277	42.18689184708703	17.877593384257338
19	26.34123995521592	24.20358496882253	30.108164125004606	19.347010950956946
20	29.25749084216815	22.335179048226177	32.4484366415784	15.958893468027274
21	18.310217849883635	32.65313640724682	33.12692506675167	15.90972067611788
22	20.262393705882637	23.83647083508855	27.981240659972546	27.919894799056266
23	15.094765740499	32.76333471615126	28.9047921848897	23.237107358460044
24	14.133413632876257	22.646713511626082	47.99520765493834	15.22466520055932
25	13.364748393875663	26.244335919042683	43.11957317375935	17.271342513322303
26	13.110395320415998	36.89272803281603	31.605771956772795	18.391104689995178
27	12.600567969772348	42.86778285166955	29.27719199332403	15.254457185234067
28	12.249310860139445	32.32126010484856	40.84144737790492	14.587981657107072
29	12.845470897555616	24.582231483720925	40.827352245370626	21.74494537335283
30	14.486272462115327	38.13021660054234	31.53721835762875	15.84629257971358
31	26.541935421868917	30.825414164647164	28.0915991408376	14.541051272646314
32	29.071691367852527	24.84106937207786	31.74303932702149	14.344199933048122
33	29.139283935232868	28.00750886151372	26.554589006757656	16.298618196495756
34	17.896493675610134	26.985611752777782	29.061760706294276	26.056133865317804
35	17.446570638237212	25.435307345966628	36.89448992438282	20.22363209141334
36	31.200857240333224	25.995909208125845	27.438097541520577	15.365136010020358
37	16.554893333482827	37.33368144039441	31.95686889444508	14.15455633167769
38	16.20651931914103	36.53762679612832	28.583807575813392	18.672046308917253
39	17.235944510025963	35.841038939405344	30.771756557840497	16.151259992728193
40	25.793451849906056	25.41592653873198	28.482098380821654	20.308523230540306
41	14.399459259460956	24.641334937188567	31.62146880891325	29.33773699443723
42	16.16711701682927	23.09583568919592	30.47095361580193	30.266093678172883
43	16.823021195555548	24.313783277726966	31.04132596755877	27.821869559158714
44	13.919423893492052	28.798598175000677	34.98395877814421	22.29801915336305
45	13.54013669075119	42.55128305749052	26.183150230087023	17.725430021671265
46	19.03884009873	38.321141577597714	26.641402209412025	15.998616114260269
47	17.140161677577044	28.77633427247493	29.45306080608141	24.630443243866615
48	17.27614767214081	26.53184458835005	38.52167687229009	17.670330867219047
49	20.292986550360467	25.786404283638912	36.974736076651894	16.945873089348726
50	18.4273035530946	35.544080124421576	29.632773745893594	16.395842576590226
51	15.52034263985815	35.37301647048271	27.63879300817357	21.467847881485564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	438.0
1	983.0
2	1528.0
3	5235.0
4	8942.0
5	6477.0
6	4012.0
7	4114.5
8	4217.0
9	4318.0
10	4419.0
11	4367.5
12	4316.0
13	4167.0
14	4018.0
15	3859.5
16	3701.0
17	3411.0
18	3121.0
19	3192.5
20	3264.0
21	3327.5
22	3391.0
23	3531.5
24	3672.0
25	4581.5
26	6626.5
27	7762.0
28	9439.5
29	11117.0
30	13664.0
31	16211.0
32	19457.5
33	22704.0
34	25184.0
35	27664.0
36	30486.0
37	33308.0
38	36463.5
39	39619.0
40	49064.5
41	58510.0
42	68862.0
43	79214.0
44	78423.5
45	77633.0
46	70672.5
47	63712.0
48	53340.0
49	42968.0
50	36488.0
51	30008.0
52	24856.0
53	19704.0
54	15533.5
55	11363.0
56	9484.5
57	7606.0
58	6984.0
59	6362.0
60	5662.5
61	4963.0
62	4338.0
63	3713.0
64	2970.0
65	2227.0
66	1782.0
67	1337.0
68	1124.0
69	911.0
70	704.5
71	498.0
72	389.0
73	280.0
74	209.0
75	131.0
76	124.0
77	95.0
78	66.0
79	52.5
80	39.0
81	29.5
82	20.0
83	17.0
84	14.0
85	7.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	624329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.921000724024935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.34037300748588	19.78818863999375
2	8.189732469980623	4.245721225678764
3	3.3650501166871924	2.6167639953308672
4	1.903489294680185	1.9736138954231515
5	1.2754705074269244	1.6530735973242876
6	0.9954418283917034	1.5481709012679628
7	0.7814091850335214	1.4178435635709548
8	0.6031784008683604	1.2507990212499975
9	0.48339375416029545	1.1277044866420334
>10	4.374982641930881	23.291649886201856
>50	1.1713382864041133	22.164774946129747
>100	0.5136349705087555	17.8332006815021
>500	0.0012527682207530623	0.2594593175978327
>1k	0.0012527682207530623	0.8290358420866919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4088	0.654782975001962	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1018	0.16305505590802286	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	841	0.1347046188788283	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	757	0.12125017418700716	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.610317637015099E-4	0.0	0.0	0.06615101973478726	0.0
2	9.610317637015099E-4	0.0	0.0	0.2194355860451781	0.0
3	9.610317637015099E-4	0.0	0.0	0.2891103889135376	0.0
4	9.610317637015099E-4	0.0	0.0	0.5067840833919296	0.0
5	9.610317637015099E-4	0.0	0.0	0.809829432879139	0.0
6	9.610317637015099E-4	0.0	0.0	1.1887962916987678	0.0
7	9.610317637015099E-4	0.0	0.0	1.4008639675555676	0.0
8	9.610317637015099E-4	0.0	0.0	1.8522285525740434	0.0
9	9.610317637015099E-4	0.0	0.0	1.9893357508621257	0.0
10	9.610317637015099E-4	0.0	0.0	2.364938998508799	0.0
11	9.610317637015099E-4	0.0	0.0	2.7403820741948555	0.0
12	9.610317637015099E-4	0.0	0.0	3.10605466028328	0.0
13	9.610317637015099E-4	0.0	0.0	3.247166157586785	0.0
14	9.610317637015099E-4	0.0	0.0	3.3029059998814727	0.0
15	9.610317637015099E-4	0.0	0.0	3.375143554119703	0.0
16	9.610317637015099E-4	0.0	0.0	3.5582201051048408	0.0
17	9.610317637015099E-4	0.0	0.0	3.761798667048944	0.0
18	9.610317637015099E-4	0.0	0.0	4.002857467777406	0.0
19	0.0011212037243184282	0.0	0.0	4.1530987668360755	0.0
20	0.0011212037243184282	0.0	0.0	4.313430899413611	0.0
21	0.0011212037243184282	0.0	0.0	4.539593707804699	0.0
22	0.0011212037243184282	0.0	0.0	4.772964254423549	0.0
23	0.0011212037243184282	0.0	0.0	5.058871204124748	0.0
24	0.0011212037243184282	0.0	0.0	5.243068958834204	0.0
25	0.0011212037243184282	0.0	0.0	5.400037480238784	0.0
26	0.0011212037243184282	0.0	0.0	5.554923766155344	0.0
27	0.0011212037243184282	0.0	0.0	5.7008404222773565	0.0
28	0.0012813756849353467	0.0	0.0	5.869821840728206	0.0
29	0.0012813756849353467	0.0	0.0	6.04633134132805	0.0
30	0.0012813756849353467	0.0	0.0	6.268169506782482	0.0
31	0.0016017196061691831	0.0	0.0	6.473349788332754	0.0
32	0.0016017196061691831	0.0	0.0	6.651461008538767	0.0
33	0.0016017196061691831	0.0	0.0	6.843347017357836	0.0
34	0.0016017196061691831	0.0	0.0	7.036194057940605	0.0
35	0.0016017196061691831	0.0	0.0	7.286062316502997	0.0
36	0.0016017196061691831	0.0	0.0	7.492523973738205	0.0
37	0.0016017196061691831	0.0	0.0	7.709076464492279	0.0
38	0.0016017196061691831	0.0	0.0	7.925468783285735	0.0
39	0.0016017196061691831	0.0	0.0	8.1362550834576	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	40	6.8066583E-9	45.000004	6
CGGGTCC	30	2.1639025E-6	45.000004	6
GGTACCG	20	7.030807E-4	45.000004	9
TCGCAAA	20	7.030807E-4	45.000004	1
CGAACGG	20	7.030807E-4	45.000004	2
AGTCACG	20	7.030807E-4	45.000004	1
GTCGAAG	20	7.030807E-4	45.000004	1
GCGAAAG	40	6.8066583E-9	45.000004	1
AACCCAT	20	7.030807E-4	45.000004	38
GACGTAT	20	7.030807E-4	45.000004	9
CAATTGC	20	7.030807E-4	45.000004	25
CACGAGC	20	7.030807E-4	45.000004	36
ACGGGTC	20	7.030807E-4	45.000004	5
ACGGGCC	30	2.1639025E-6	45.000004	5
CATTACG	20	7.030807E-4	45.000004	38
TATTGCG	20	7.030807E-4	45.000004	1
CCGTATG	30	2.1639025E-6	45.000004	1
TAACCGA	20	7.030807E-4	45.000004	21
TCTACGG	30	2.1639025E-6	45.000004	2
CTAGTCC	20	7.030807E-4	45.000004	42
>>END_MODULE