FastQCFastQC Report
Sat 18 Jun 2016
SRR3549339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250423
Sequences flagged as poor quality0
Sequence length51
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.48637705003134696No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.23879595724034933No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3190.1273844654844004No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTA207.0227083E-445.00000411
GGTACCC207.0227083E-445.0000048
GTACCCA207.0227083E-445.0000049
TACCCAG207.0227083E-445.00000410
CGTATGT207.0227083E-445.00000440
ATTAGGC302.1586802E-645.0000044
TTTACGT207.0227083E-445.00000430
CCCTAGC800.045.00000421
CCCTACA800.045.00000427
CTTCACC207.0227083E-445.00000411
GGCGTAG207.0227083E-445.0000041
CCGTGCC207.0227083E-445.00000432
CACGAGC207.0227083E-445.00000436
GCGTTAG302.1586802E-645.0000041
GCGCAGG302.1586802E-645.0000042
ACGGGCG207.0227083E-445.0000045
TGGCATC207.0227083E-445.00000412
CGTTAAG302.1586802E-645.0000042
AACGGGA302.1586802E-645.0000044
TCAATCT302.1586802E-645.00000429