##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	250423
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.691949221916516	31.0	31.0	33.0	30.0	34.0
2	32.037640312591094	31.0	31.0	34.0	30.0	34.0
3	31.90975269843425	33.0	31.0	34.0	30.0	34.0
4	35.73929710929108	37.0	35.0	37.0	35.0	37.0
5	35.73387428471027	37.0	35.0	37.0	35.0	37.0
6	35.87999504837814	37.0	35.0	37.0	35.0	37.0
7	23.023576109223196	35.0	0.0	37.0	0.0	37.0
8	29.03061220415058	35.0	17.0	37.0	17.0	37.0
9	35.36140450357994	37.0	32.0	39.0	32.0	39.0
10	36.52805053848888	37.0	35.0	39.0	32.0	39.0
11	37.03569959628309	39.0	37.0	39.0	34.0	39.0
12	36.662614855664216	39.0	35.0	39.0	33.0	39.0
13	36.15167935852538	38.0	35.0	39.0	32.0	39.0
14	37.40963889099643	39.0	35.0	41.0	33.0	41.0
15	37.6658453896008	40.0	35.0	41.0	33.0	41.0
16	37.7995471661948	40.0	35.0	41.0	33.0	41.0
17	37.88116906194718	40.0	35.0	41.0	34.0	41.0
18	37.8523458308542	39.0	36.0	41.0	34.0	41.0
19	37.808228477416215	39.0	36.0	41.0	34.0	41.0
20	37.670477551982046	39.0	35.0	41.0	34.0	41.0
21	37.552676870734714	39.0	35.0	41.0	34.0	41.0
22	37.435231588152845	39.0	35.0	41.0	33.0	41.0
23	37.489567651533605	39.0	35.0	41.0	34.0	41.0
24	37.36281411851148	39.0	35.0	41.0	34.0	41.0
25	37.167935852537504	38.0	35.0	40.0	33.0	41.0
26	37.080088490274456	38.0	35.0	40.0	33.0	41.0
27	36.941059726942015	38.0	35.0	40.0	33.0	41.0
28	37.067066523442335	38.0	35.0	40.0	33.0	41.0
29	36.94858299756812	38.0	35.0	40.0	33.0	41.0
30	36.759494934570704	38.0	35.0	40.0	33.0	41.0
31	36.46109183261921	38.0	35.0	40.0	32.0	41.0
32	36.186592285852335	38.0	35.0	40.0	31.0	41.0
33	35.98714574939203	38.0	35.0	40.0	31.0	41.0
34	35.699879803372696	38.0	35.0	40.0	30.0	41.0
35	35.56018816163052	38.0	35.0	40.0	30.0	41.0
36	35.33014539399336	38.0	35.0	40.0	29.0	41.0
37	35.21865004412534	38.0	34.0	40.0	29.0	41.0
38	35.047847042803575	37.0	34.0	40.0	28.0	41.0
39	34.94157086210132	37.0	34.0	40.0	27.0	41.0
40	34.71499023651981	37.0	34.0	40.0	27.0	40.0
41	34.568170655251315	37.0	34.0	40.0	26.0	40.0
42	34.52238811930214	37.0	34.0	40.0	25.0	40.0
43	34.38873026838589	36.0	34.0	40.0	25.0	40.0
44	34.173598271724245	36.0	34.0	39.0	24.0	40.0
45	33.95178557880067	36.0	33.0	39.0	24.0	40.0
46	33.94854306513379	36.0	33.0	39.0	24.0	40.0
47	33.881173055190615	36.0	33.0	39.0	24.0	40.0
48	33.71314535805417	35.0	33.0	39.0	23.0	40.0
49	33.627011097223495	36.0	33.0	39.0	24.0	40.0
50	33.53307004548304	35.0	33.0	39.0	23.0	40.0
51	33.762925130678894	36.0	33.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	0.0
15	0.0
16	9.0
17	28.0
18	53.0
19	133.0
20	235.0
21	497.0
22	740.0
23	1215.0
24	2020.0
25	2926.0
26	3729.0
27	4382.0
28	4377.0
29	4707.0
30	5312.0
31	6770.0
32	8935.0
33	14247.0
34	25804.0
35	34501.0
36	24209.0
37	45423.0
38	40776.0
39	19392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.319191927259077	24.31925182591056	30.8414163235805	15.520139923249863
2	30.770735914832102	24.91344644860895	31.624491360617835	12.691326275941108
3	27.524628328867557	24.496951158639583	35.060677333950956	12.917743178541908
4	24.171501818922383	28.12800741145981	34.05877255683384	13.641718212783971
5	21.952456443697262	31.59174676447451	32.558510999389036	13.897285792439193
6	21.21530370612923	40.258682309532276	29.211773678935245	9.314240305403258
7	55.52565059918617	37.4538281228162	5.837722573405797	1.1827987045918305
8	86.760800725173	3.133897445522176	7.932977402235418	2.1723244270693987
9	83.03350730563885	4.932054963002599	9.516298423068168	2.5181393082903725
10	49.09692799782768	28.60360270422445	14.144467560886978	8.155001737060893
11	45.09929199793949	17.895720441013804	25.69452486393023	11.31046269711648
12	43.0160168994062	19.10447522791437	26.110620829556392	11.768887043123035
13	19.47984011053298	42.18861686027242	25.661780267786906	12.6697627614077
14	13.220830355039274	43.853400047120275	28.567264188992226	14.35850540884823
15	11.580006628784096	25.43456471649968	50.278129405046656	12.70729924966956
16	13.470008745203115	20.591958406376413	49.88918749475887	16.048845353661605
17	13.186887785866313	21.910128063316865	30.706444695575087	34.196539455241734
18	15.39235613342225	24.319651150253772	42.5276432276588	17.760349488665177
19	26.483589766115735	24.168307224176694	29.971687904066318	19.376415105641254
20	29.53123315350427	22.314244298646692	32.25063193077313	15.903890617075907
21	18.34096708369439	32.90512452929643	32.831648850145555	15.922259536863626
22	20.160288791365012	23.77617071914321	27.77660198943388	28.2869385000579
23	14.983048681630681	32.960231288659585	28.57804594625893	23.4786740834508
24	13.965969579471535	22.76188688738654	48.12018065433287	15.151962878809055
25	13.190082380612003	26.151751236907156	43.398569620202615	17.259596762278225
26	12.972051289218642	37.1355666212768	31.485526489180305	18.406855600324253
27	12.440949912747632	43.409750701812534	28.856774337820408	15.29252504761943
28	12.04322286690919	32.387200856151395	40.894406663924634	14.675169613014779
29	12.722074250368376	24.479780211881497	40.97027828913478	21.827867248615345
30	14.419602033359554	38.432172763683845	31.299441345243846	15.84878385771275
31	26.710405993059744	31.041877143872565	27.826118207992078	14.421598655075613
32	29.44178450062494	24.870718743885345	31.43002040547394	14.257476350015775
33	29.348342604313505	28.096460788346118	26.340631651246092	16.21456495609429
34	17.724010973432954	26.982345870786627	28.961796640084973	26.331846515695446
35	17.34185757697975	25.354300523514212	37.05490310394812	20.248938795557915
36	31.403664998821995	25.94330392975086	27.31977494080017	15.33325613062698
37	16.473327130495203	37.435059878685266	31.972302863554862	14.119310127264667
38	16.0664156247629	36.79454363217436	28.41631958725836	18.72272115580438
39	17.0994677006505	36.12886995204115	30.616596718352547	16.155065628955807
40	26.07268501695132	25.288811331227564	28.418316208974414	20.220187442846704
41	14.22912432164777	24.684234275605675	31.453181217380195	29.63346018536636
42	16.02927846084425	22.902848380540124	30.354639949205943	30.71323320940968
43	16.648630517164957	24.122384924707397	31.273085938591905	27.955898619535745
44	13.72278105445586	28.621971624012172	35.07385503727693	22.581392284255042
45	13.258366843301134	42.8391162153636	26.209653266672788	17.69286367466247
46	19.06733806399572	38.44574979135303	26.545085714970266	15.94182642968098
47	16.837910255847106	28.72459798021747	29.49050207049672	24.946989693438702
48	17.07431026702819	26.511941794483736	38.69452885717366	17.719219081314417
49	20.25972055282462	25.41180322893664	37.54287745135231	16.785598766886427
50	18.38529208579084	35.71556925681747	29.539219640368497	16.3599190170232
51	15.281743290352722	35.69280776925442	27.380871565311494	21.64457737508136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	188.0
1	370.5
2	553.0
3	1909.5
4	3266.0
5	2414.5
6	1563.0
7	1596.5
8	1630.0
9	1651.5
10	1673.0
11	1683.5
12	1694.0
13	1632.5
14	1571.0
15	1503.5
16	1436.0
17	1340.0
18	1244.0
19	1322.5
20	1401.0
21	1420.0
22	1439.0
23	1593.5
24	1748.0
25	2164.0
26	3024.0
27	3468.0
28	4366.0
29	5264.0
30	6250.5
31	7237.0
32	8455.5
33	9674.0
34	10890.0
35	12106.0
36	12965.5
37	13825.0
38	15918.0
39	18011.0
40	21759.5
41	25508.0
42	28209.5
43	30911.0
44	30243.0
45	29575.0
46	26658.5
47	23742.0
48	19904.0
49	16066.0
50	13626.0
51	11186.0
52	9104.5
53	7023.0
54	5669.5
55	4316.0
56	3637.5
57	2959.0
58	2666.5
59	2374.0
60	2105.0
61	1836.0
62	1595.0
63	1354.0
64	1074.5
65	795.0
66	631.5
67	468.0
68	399.0
69	330.0
70	254.0
71	178.0
72	141.5
73	105.0
74	81.5
75	47.0
76	36.0
77	27.5
78	19.0
79	14.0
80	9.0
81	5.0
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	250423.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.70417254006222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93931495325997	24.920634286786758
2	8.267478644124024	5.572970533856714
3	3.7818560951624938	3.82392991059128
4	2.220300226295274	2.9933352767118038
5	1.3826522753930548	2.330057542637857
6	1.0710519768254683	2.1659352375780183
7	0.8400175349217445	1.9818467153576147
8	0.709690413847849	1.913562252668485
9	0.6243853891449356	1.8939953598511319
>10	7.002120777697475	47.91532726626548
>50	0.14454462519104772	3.024881899825495
>100	0.014217504117152233	0.7383507105976688
>500	0.0011847920097626862	0.23879595724034933
>1k	0.0011847920097626862	0.48637705003134696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.48637705003134696	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	598	0.23879595724034933	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	319	0.1273844654844004	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0011979730296338596	0.0	0.0	0.05710338107921397	0.0
2	0.0011979730296338596	0.0	0.0	0.19766554988958682	0.0
3	0.0011979730296338596	0.0	0.0	0.25876217440091365	0.0
4	0.0011979730296338596	0.0	0.0	0.47080340064610676	0.0
5	0.0011979730296338596	0.0	0.0	0.767900711995304	0.0
6	0.0011979730296338596	0.0	0.0	1.0482264009296272	0.0
7	0.0011979730296338596	0.0	0.0	1.2079561382141417	0.0
8	0.0011979730296338596	0.0	0.0	1.7202892705542223	0.0
9	0.0011979730296338596	0.0	0.0	1.8612507637078064	0.0
10	0.0011979730296338596	0.0	0.0	2.2753501076179106	0.0
11	0.0011979730296338596	0.0	0.0	2.720596750298495	0.0
12	0.0011979730296338596	0.0	0.0	3.0979582546331605	0.0
13	0.0011979730296338596	0.0	0.0	3.2429129912188577	0.0
14	0.0011979730296338596	0.0	0.0	3.300815020984494	0.0
15	0.0011979730296338596	0.0	0.0	3.3894650251773997	0.0
16	0.0011979730296338596	0.0	0.0	3.5867312507237754	0.0
17	0.0011979730296338596	0.0	0.0	3.791185314447954	0.0
18	0.0011979730296338596	0.0	0.0	4.05673600268346	0.0
19	0.0011979730296338596	0.0	0.0	4.232838038039637	0.0
20	0.0011979730296338596	0.0	0.0	4.406943451679758	0.0
21	0.0011979730296338596	0.0	0.0	4.662511031334981	0.0
22	0.0011979730296338596	0.0	0.0	4.90410225897781	0.0
23	0.0011979730296338596	0.0	0.0	5.204793489415908	0.0
24	0.0011979730296338596	0.0	0.0	5.412442147885777	0.0
25	0.0011979730296338596	0.0	0.0	5.570574587797447	0.0
26	0.0011979730296338596	0.0	0.0	5.734297568514074	0.0
27	0.0011979730296338596	0.0	0.0	5.892030684082532	0.0
28	0.0011979730296338596	0.0	0.0	6.076917854989358	0.0
29	0.0011979730296338596	0.0	0.0	6.259808404180127	0.0
30	0.0011979730296338596	0.0	0.0	6.506590848284702	0.0
31	0.0011979730296338596	0.0	0.0	6.712642209381726	0.0
32	0.0011979730296338596	0.0	0.0	6.9095091105848905	0.0
33	0.0011979730296338596	0.0	0.0	7.105577363101632	0.0
34	0.0011979730296338596	0.0	0.0	7.293659128754148	0.0
35	0.0011979730296338596	0.0	0.0	7.5528206274982725	0.0
36	0.0011979730296338596	0.0	0.0	7.778039557069438	0.0
37	0.0011979730296338596	0.0	0.0	8.00206051361097	0.0
38	0.0011979730296338596	0.0	0.0	8.23167201095746	0.0
39	0.0011979730296338596	0.0	0.0	8.469669319511386	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTTA	20	7.0227083E-4	45.000004	11
GGTACCC	20	7.0227083E-4	45.000004	8
GTACCCA	20	7.0227083E-4	45.000004	9
TACCCAG	20	7.0227083E-4	45.000004	10
CGTATGT	20	7.0227083E-4	45.000004	40
ATTAGGC	30	2.1586802E-6	45.000004	4
TTTACGT	20	7.0227083E-4	45.000004	30
CCCTAGC	80	0.0	45.000004	21
CCCTACA	80	0.0	45.000004	27
CTTCACC	20	7.0227083E-4	45.000004	11
GGCGTAG	20	7.0227083E-4	45.000004	1
CCGTGCC	20	7.0227083E-4	45.000004	32
CACGAGC	20	7.0227083E-4	45.000004	36
GCGTTAG	30	2.1586802E-6	45.000004	1
GCGCAGG	30	2.1586802E-6	45.000004	2
ACGGGCG	20	7.0227083E-4	45.000004	5
TGGCATC	20	7.0227083E-4	45.000004	12
CGTTAAG	30	2.1586802E-6	45.000004	2
AACGGGA	30	2.1586802E-6	45.000004	4
TCAATCT	30	2.1586802E-6	45.000004	29
>>END_MODULE