##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1121302
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980467349563277	33.0	31.0	34.0	31.0	34.0
2	32.28850300811021	33.0	31.0	34.0	31.0	34.0
3	32.22628872507139	33.0	31.0	34.0	30.0	34.0
4	35.985029010917664	37.0	35.0	37.0	35.0	37.0
5	32.42512097543748	37.0	35.0	37.0	10.0	37.0
6	34.193517892592716	37.0	35.0	37.0	19.0	37.0
7	35.514406466768094	37.0	35.0	37.0	32.0	37.0
8	35.74763979730706	37.0	35.0	37.0	35.0	37.0
9	38.00501738157963	39.0	38.0	39.0	35.0	39.0
10	37.65158182184639	39.0	37.0	39.0	35.0	39.0
11	37.52613122958846	39.0	37.0	39.0	35.0	39.0
12	36.824817934865	39.0	35.0	39.0	34.0	39.0
13	36.567912123584904	39.0	35.0	39.0	33.0	39.0
14	37.72087805069464	40.0	35.0	41.0	33.0	41.0
15	37.916580011450975	40.0	35.0	41.0	33.0	41.0
16	37.96985201132255	40.0	35.0	41.0	34.0	41.0
17	37.94460279211131	40.0	35.0	41.0	34.0	41.0
18	37.945049594132534	39.0	36.0	41.0	34.0	41.0
19	37.81861175668999	39.0	36.0	41.0	34.0	41.0
20	37.69305682144507	39.0	35.0	41.0	34.0	41.0
21	37.553995266217306	39.0	35.0	41.0	34.0	41.0
22	37.43713022896597	39.0	35.0	41.0	34.0	41.0
23	37.445080807846594	39.0	35.0	41.0	34.0	41.0
24	37.38470991757796	39.0	35.0	41.0	34.0	41.0
25	37.24174129717061	38.0	35.0	41.0	34.0	41.0
26	37.180282386011974	38.0	35.0	41.0	34.0	41.0
27	36.13143203169173	38.0	35.0	41.0	33.0	41.0
28	36.52489070740978	38.0	35.0	40.0	33.0	41.0
29	36.815586701887625	38.0	35.0	40.0	33.0	41.0
30	36.74616561818315	38.0	35.0	40.0	33.0	41.0
31	36.565439105611155	38.0	35.0	40.0	33.0	41.0
32	36.32072269558067	38.0	35.0	40.0	32.0	41.0
33	36.099569072381925	38.0	35.0	40.0	31.0	41.0
34	35.90698937485173	38.0	35.0	40.0	31.0	41.0
35	35.729043558292055	38.0	35.0	40.0	31.0	41.0
36	35.52197713015762	38.0	35.0	40.0	30.0	41.0
37	35.40934021343046	38.0	35.0	40.0	30.0	41.0
38	35.25950814321209	37.0	34.0	40.0	30.0	41.0
39	35.21857715405841	37.0	35.0	40.0	29.0	41.0
40	35.01013910614625	37.0	34.0	40.0	29.0	41.0
41	34.865204021753286	37.0	34.0	40.0	28.0	41.0
42	34.861827589712675	37.0	34.0	40.0	27.0	41.0
43	34.75286497304027	36.0	34.0	40.0	27.0	41.0
44	34.608535434700016	36.0	34.0	40.0	27.0	40.0
45	34.49934362018439	36.0	34.0	40.0	27.0	40.0
46	34.40696707934169	36.0	34.0	40.0	26.0	40.0
47	34.25262864063384	36.0	34.0	39.0	26.0	40.0
48	34.22002636221107	36.0	34.0	39.0	26.0	40.0
49	34.09572265098965	36.0	34.0	39.0	25.0	40.0
50	33.98963615511254	35.0	34.0	39.0	24.0	40.0
51	34.12458552646834	36.0	34.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	4.0
14	6.0
15	15.0
16	50.0
17	131.0
18	310.0
19	676.0
20	1188.0
21	2020.0
22	3290.0
23	4909.0
24	7377.0
25	11403.0
26	16064.0
27	17728.0
28	17042.0
29	17073.0
30	19325.0
31	24196.0
32	33783.0
33	50516.0
34	102135.0
35	183359.0
36	73283.0
37	103311.0
38	205531.0
39	226572.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.54797191122463	24.042853753939617	30.652045568455243	15.757128766380509
2	31.408576815166654	25.506152668950914	30.524693615101018	12.560576900781415
3	26.77048645235628	24.31093496667267	36.0251743062975	12.893404274673548
4	23.8106237213525	28.28399485597992	34.01108711123319	13.894294311434386
5	19.657772839074575	39.266584738099105	29.431589348810576	11.644053074015742
6	20.47815842654343	40.79784036771539	28.92494617863876	9.799055027102423
7	85.63964034666841	3.846332210234174	8.988390282011448	1.5256371610859518
8	86.02169620673111	3.5895771166019506	8.465248434409284	1.923478242257661
9	81.3720121786994	6.396225102603938	9.914367405034504	2.3173953136621535
10	43.89424080221029	31.88257935863844	15.946729783769225	8.276450055382046
11	40.421402976183046	18.69300152858017	28.3499895657013	12.535605929535487
12	42.1718680605225	18.73554136173841	27.016539701168817	12.076050876570273
13	18.60016302476942	42.16286067446593	25.158164348230894	14.078811952533751
14	12.028695213243177	41.93393037736489	28.563848098014628	17.47352631137731
15	11.346274241908068	27.405730124444617	46.65451412732698	14.59348150632033
16	11.315684802131807	22.432939564898664	47.845183545556864	18.406192087412666
17	12.423058194848489	24.08967432502573	31.216032790452523	32.27123468967326
18	14.597583880167878	26.602110760526603	40.677444613493954	18.122860745811565
19	23.33430244483645	27.400914294275765	29.301026842010447	19.96375641887734
20	25.567777458704256	23.24636895323472	34.65872708690433	16.52712650115669
21	15.926128732491335	33.60218745708114	33.70447925714928	16.76720455327824
22	19.95109256917405	26.79536824156204	26.012706657082568	27.24083253218134
23	15.210710406295538	33.29361759811362	28.093412836149405	23.402259159441435
24	12.310867188322147	24.36052018100387	48.63917124913716	14.689441381536822
25	12.182534232526116	30.010113243354603	40.025613081935106	17.781739442184175
26	12.279831838345066	38.03105675366672	30.721518377743017	18.967593030245197
27	11.077301208773372	43.78106879324214	28.898726658830537	16.242903339153948
28	10.55924273746056	33.5484998689024	40.387781347041205	15.504476046595833
29	11.41503359487453	24.820253598049412	39.884616276435786	23.880096530640273
30	13.592948197720151	40.29877767095751	31.105357878608974	15.002916252713364
31	23.9534041676551	33.34587827364974	28.289613324510256	14.4111042341849
32	25.969542549643183	26.431505517692827	31.143081881598356	16.455870051065634
33	26.031702431637505	30.286220839702416	25.604074549051013	18.07800217960906
34	16.415738132991827	28.30718218642257	28.429807491648102	26.847272188937506
35	18.012631744168832	25.87929032499719	35.23760770960901	20.87047022122497
36	29.265799936145658	27.578564918282495	26.16850768124912	16.987127464322725
37	15.571184212638522	36.56222855216525	33.141027127392974	14.72556010780325
38	14.876188573640286	35.266502690622154	31.002798532420346	18.854510203317215
39	16.63039930366663	37.08296248468299	30.339819245840992	15.94681896580939
40	24.554401936320456	28.09439383859121	28.482424895344877	18.86877932974346
41	14.031902199407476	25.72652149019622	32.72659818675076	27.51497812364555
42	15.076402253808519	24.506511180752373	30.18312640127281	30.2339601641663
43	16.377835765922118	26.325913982138623	31.07450089271222	26.221749359227044
44	13.701928650800587	30.455577533973898	33.07360550502897	22.768888310196537
45	13.385154044137975	43.12995071800461	24.570989795790965	18.913905442066454
46	17.316030828447644	39.68859415215526	26.35605751171406	16.63931750768303
47	16.250127084407236	29.79509534451914	31.313954670552626	22.640822900521
48	16.31300042272287	27.693788114174417	38.363081489197384	17.630129973905333
49	18.708786749689203	28.924767814558432	35.126576069604795	17.239869366147566
50	17.642169549327477	35.87615111718342	30.635368526944568	15.846310806544533
51	15.697555163550945	33.97479002088644	28.195615454177375	22.132039361385246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	710.0
1	1683.0
2	2656.0
3	9923.5
4	17191.0
5	11920.0
6	6649.0
7	7053.0
8	7457.0
9	8153.0
10	8849.0
11	9310.5
12	9772.0
13	9680.0
14	9588.0
15	8783.0
16	7978.0
17	7899.0
18	7820.0
19	7243.0
20	6666.0
21	7134.0
22	7602.0
23	7598.0
24	7594.0
25	9118.0
26	14545.5
27	18449.0
28	21709.5
29	24970.0
30	30244.0
31	35518.0
32	42348.0
33	49178.0
34	56414.5
35	63651.0
36	61145.0
37	58639.0
38	67939.0
39	77239.0
40	89057.0
41	100875.0
42	114943.0
43	129011.0
44	124739.5
45	120468.0
46	104409.5
47	88351.0
48	80504.5
49	72658.0
50	66421.0
51	60184.0
52	51017.5
53	41851.0
54	32187.5
55	22524.0
56	17658.5
57	12793.0
58	11061.5
59	9330.0
60	8228.0
61	7126.0
62	6275.0
63	5424.0
64	4658.0
65	3892.0
66	3297.0
67	2702.0
68	2339.5
69	1977.0
70	1608.0
71	1239.0
72	1100.0
73	961.0
74	678.0
75	369.5
76	344.0
77	279.5
78	215.0
79	152.5
80	90.0
81	58.5
82	27.0
83	26.0
84	25.0
85	21.0
86	17.0
87	10.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1121302.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.255610248805805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4227117417271	16.456669851877923
2	8.442530900234365	3.5890229265776257
3	3.138752296999977	2.0014828647772647
4	1.7159394715724159	1.4589336247314026
5	1.0582915684821061	1.1247316554626514
6	0.7537916786429796	0.961338127801695
7	0.611152196253688	0.9093289020389047
8	0.4903856005904178	0.833875615822118
9	0.4305628530814758	0.8236688573442319
>10	3.8956860459101814	16.094974414821642
>50	0.7968784162263503	12.474811518882001
>100	1.2373703764272936	41.193892350825166
>500	0.004672528026330323	0.6539601625808535
>1k	8.495505502418769E-4	0.45541558194916826
>5k	0.0	0.0
>10k+	4.2477527512093847E-4	0.967893544507352
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10720	0.9560314705583332	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3941	0.35146642028641706	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7836408032804722E-4	0.0	0.0	0.04940685025086908	0.0
2	1.7836408032804722E-4	0.0	0.0	0.1827340002960844	0.0
3	1.7836408032804722E-4	0.0	0.0	0.24346696964778444	0.0
4	1.7836408032804722E-4	0.0	0.0	0.39971390401515383	0.0
5	1.7836408032804722E-4	0.0	0.0	0.6423782353014621	0.0
6	1.7836408032804722E-4	0.0	0.0	0.9490752714255393	0.0
7	1.7836408032804722E-4	0.0	0.0	1.125655710950306	0.0
8	1.7836408032804722E-4	0.0	0.0	1.5234967921220153	0.0
9	1.7836408032804722E-4	0.0	0.0	1.6768007191639718	0.0
10	1.7836408032804722E-4	0.0	0.0	2.062334678793046	0.0
11	1.7836408032804722E-4	0.0	0.0	2.6370237456100143	0.0
12	1.7836408032804722E-4	0.0	0.0	3.071964555489957	0.0
13	1.7836408032804722E-4	0.0	0.0	3.2522014586614487	0.0
14	1.7836408032804722E-4	0.0	0.0	3.324617275274636	0.0
15	1.7836408032804722E-4	0.0	0.0	3.4163855946034163	0.0
16	1.7836408032804722E-4	0.0	0.0	3.6169560029323056	0.0
17	1.7836408032804722E-4	0.0	0.0	3.8319739017677663	0.0
18	1.7836408032804722E-4	0.0	0.0	4.084537439512281	0.0
19	1.7836408032804722E-4	0.0	0.0	4.2297258008993115	0.0
20	1.7836408032804722E-4	0.0	0.0	4.363498861145347	0.0
21	1.7836408032804722E-4	0.0	0.0	4.5725415632898185	0.0
22	1.7836408032804722E-4	0.0	0.0	4.776946799345761	0.0
23	1.7836408032804722E-4	0.0	0.0	5.005163640125497	0.0
24	1.7836408032804722E-4	0.0	0.0	5.171398962991237	0.0
25	1.7836408032804722E-4	0.0	0.0	5.307134028120881	0.0
26	1.7836408032804722E-4	0.0	0.0	5.437161442680027	0.0
27	1.7836408032804722E-4	0.0	0.0	5.559073291584248	0.0
28	1.7836408032804722E-4	0.0	0.0	5.69632445139668	0.0
29	1.7836408032804722E-4	0.0	0.0	5.837321256896002	0.0
30	1.7836408032804722E-4	0.0	0.0	6.0231766285978265	0.0
31	2.675461204920708E-4	0.0	0.0	6.190660500025863	0.0
32	2.675461204920708E-4	0.0	0.0	6.344231973188311	0.0
33	2.675461204920708E-4	0.0	0.0	6.4913823394589505	0.0
34	2.675461204920708E-4	0.0	0.0	6.645578086902547	0.0
35	2.675461204920708E-4	0.0	0.0	6.866303636308506	0.0
36	3.5672816065609444E-4	0.0	0.0	7.03619542282097	0.0
37	3.5672816065609444E-4	0.0	0.0	7.19886346408015	0.0
38	3.5672816065609444E-4	0.0	0.0	7.35778585965244	0.0
39	3.5672816065609444E-4	0.0	0.0	7.529015376767365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8562575E-10	45.0	25
ATCTCGA	20	7.0332136E-4	45.0	20
GTACCGG	25	3.890744E-5	45.0	2
AAATCGT	20	7.0332136E-4	45.0	12
CGAACAT	20	7.0332136E-4	45.0	12
ATCTACG	20	7.0332136E-4	45.0	1
CGACGGT	45	3.8562575E-10	45.0	28
TAAACCG	45	3.8562575E-10	45.0	39
CCAGCGG	40	6.8139343E-9	45.0	2
TTCGTAA	35	1.2119017E-7	45.0	21
CGCGAGG	85	0.0	45.0	2
GCGTTAG	40	6.8139343E-9	45.0	1
TCGATAG	45	3.8562575E-10	45.0	1
TACATCC	20	7.0332136E-4	45.0	24
TCTACGT	40	6.8139343E-9	45.0	32
CTAGTTG	100	0.0	45.0	1
CTAGTCG	20	7.0332136E-4	45.0	1
CTACGTT	40	6.8139343E-9	45.0	33
AGGTTCG	20	7.0332136E-4	45.0	1
ACGTGTC	35	1.2119017E-7	45.0	13
>>END_MODULE