##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549333_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	305898
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.721773270828837	31.0	31.0	33.0	30.0	34.0
2	32.0766268494727	33.0	31.0	34.0	30.0	34.0
3	31.97730289181361	33.0	31.0	34.0	30.0	34.0
4	35.8181943000608	37.0	35.0	37.0	35.0	37.0
5	35.8030421905341	37.0	35.0	37.0	35.0	37.0
6	35.9410032102204	37.0	35.0	37.0	35.0	37.0
7	22.53739481788047	35.0	0.0	37.0	0.0	37.0
8	28.77274777867132	35.0	17.0	37.0	17.0	37.0
9	35.32118876226716	37.0	32.0	39.0	32.0	39.0
10	36.536852153332156	37.0	35.0	39.0	33.0	39.0
11	37.11374706601547	39.0	37.0	39.0	34.0	39.0
12	36.84287572981844	39.0	35.0	39.0	33.0	39.0
13	36.38986851826426	39.0	35.0	39.0	33.0	39.0
14	37.72205767935717	40.0	35.0	41.0	33.0	41.0
15	37.96439009081458	40.0	35.0	41.0	33.0	41.0
16	38.058895448809736	40.0	35.0	41.0	34.0	41.0
17	38.151056888243794	40.0	35.0	41.0	34.0	41.0
18	38.10840868524802	40.0	36.0	41.0	34.0	41.0
19	38.06147800900954	40.0	36.0	41.0	34.0	41.0
20	37.9215032461801	40.0	35.0	41.0	34.0	41.0
21	37.82738363768315	40.0	35.0	41.0	34.0	41.0
22	37.709805229194046	39.0	35.0	41.0	34.0	41.0
23	37.74384925694186	39.0	35.0	41.0	34.0	41.0
24	37.610442042772426	39.0	35.0	41.0	34.0	41.0
25	37.408995809060535	39.0	35.0	41.0	33.0	41.0
26	37.333127382330055	39.0	35.0	40.0	33.0	41.0
27	37.202037934213365	39.0	35.0	40.0	33.0	41.0
28	37.32265003367135	39.0	35.0	40.0	33.0	41.0
29	37.244087898580574	39.0	35.0	40.0	33.0	41.0
30	37.04871558493354	38.0	35.0	40.0	33.0	41.0
31	36.77269874271816	38.0	35.0	40.0	33.0	41.0
32	36.52511621520899	38.0	35.0	40.0	32.0	41.0
33	36.36963955305363	38.0	35.0	40.0	31.0	41.0
34	36.13068081517368	38.0	35.0	40.0	31.0	41.0
35	36.00627660200459	38.0	35.0	40.0	31.0	41.0
36	35.77104786562841	38.0	35.0	40.0	30.0	41.0
37	35.64517584292803	38.0	35.0	40.0	30.0	41.0
38	35.458155986636065	38.0	34.0	40.0	30.0	41.0
39	35.33048924805	38.0	34.0	40.0	29.0	41.0
40	35.04988917874586	37.0	34.0	40.0	29.0	40.0
41	34.97693348763313	37.0	34.0	40.0	28.0	40.0
42	34.989359198164095	38.0	34.0	40.0	28.0	40.0
43	34.8654747660985	37.0	34.0	40.0	27.0	40.0
44	34.59674793558637	37.0	34.0	40.0	27.0	40.0
45	34.42367390437335	36.0	34.0	39.0	26.0	40.0
46	34.3872075005394	36.0	34.0	39.0	26.0	40.0
47	34.319675185846265	36.0	34.0	39.0	26.0	40.0
48	34.18761155679344	36.0	34.0	39.0	25.0	40.0
49	33.97610968362003	36.0	33.0	39.0	24.0	40.0
50	33.963932421918415	36.0	33.0	39.0	24.0	40.0
51	34.2590634786759	37.0	34.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	12.0
17	31.0
18	63.0
19	137.0
20	324.0
21	472.0
22	833.0
23	1399.0
24	2085.0
25	3131.0
26	4053.0
27	4336.0
28	4381.0
29	4847.0
30	5747.0
31	7399.0
32	10239.0
33	16611.0
34	29665.0
35	40079.0
36	31210.0
37	59262.0
38	53306.0
39	26272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.878586979973715	24.39440597846341	30.92534112678082	14.801665914782053
2	30.042367063530982	25.811871931166596	31.29180315006963	12.853957855232789
3	27.09530627856344	24.53301427273143	35.34380741292849	13.02787203577663
4	24.248278838044055	28.636669739586395	33.15909224643509	13.955959175934463
5	21.723254156614296	33.4915560088657	31.651727046270324	13.133462788249679
6	20.595100327560168	41.6802332803745	28.175731779874337	9.548934612190992
7	55.079470934756024	38.875049853219046	5.018339446482161	1.0271397655427628
8	87.94205911774513	3.36975070121413	6.975854696663594	1.7123354843771452
9	83.2408188350365	6.304715951068657	8.38809014769629	2.0663750661985367
10	44.90026087127082	32.60040928675571	14.400878724280641	8.098451117692825
11	41.8057653204663	18.622874291430477	27.34375510791179	12.227605280191437
12	43.541964968715064	18.965472150847667	25.618343369358413	11.874219511078858
13	18.50061131488274	43.64526737670727	23.658866681050547	14.195254627359446
14	11.680690949270673	43.40270286173823	27.370561428973055	17.546044760018045
15	11.14652596617173	27.880535341845974	46.45143152292594	14.521507169056353
16	11.138353307311588	22.447024825268553	47.714597676349634	18.700024191070224
17	12.320119778488255	24.318890610595687	30.22183865209972	33.13915095881634
18	14.739553707444966	26.9043929675905	40.252306324330334	18.1037470006342
19	24.108689824712812	27.333294104570804	28.298648569130886	20.259367501585494
20	26.261041262120056	23.416955978790316	33.8292502729668	16.492752486122825
21	15.947799593328496	34.484370607195864	32.69946191214065	16.868367887334994
22	20.212292986551073	27.09236412137379	24.669007316164212	28.026335575910927
23	15.059595028408163	34.11660095848943	27.109690158157296	23.71411385494511
24	12.024923340459893	24.35713865406116	49.00293561906256	14.615002386416387
25	12.034076718383252	30.31337243133332	39.95547535452994	17.697075495753488
26	12.342349410587843	38.91100955220367	29.72690243152946	19.019738605679017
27	11.169736317334536	43.89731217595408	28.396393569098194	16.536557937613193
28	10.54730661854605	34.16236784810623	40.09081458525391	15.199510948093808
29	11.265192972821007	25.079601697297793	39.465769635630174	24.189435694251024
30	13.296915965452536	41.470359400846036	30.242106846072875	14.990617787628555
31	24.536283336275492	33.72431333320257	27.62064479009343	14.118758540428509
32	26.835742633165303	26.326749439355602	30.540245441290885	16.297262486188206
33	26.640906445939493	30.498401427926957	24.791597199066356	18.06909492706719
34	16.276340479506242	28.382009689504343	27.85569045891114	27.485959372078273
35	18.148860077542185	25.78048892114365	35.15191338289234	20.918737618421826
36	29.958352130448713	27.648431830217916	25.550346847642025	16.84286919169135
37	15.383886131978633	37.57102040549464	32.632446109487475	14.41264735303925
38	14.713728105446913	36.250972546404356	30.38136895304971	18.65393039509902
39	16.29922392431464	38.12741502069317	29.796860391372288	15.776500663619899
40	24.89980320237465	28.281649438701788	27.98416465619259	18.834382702730977
41	13.750988891722077	25.73864490777972	32.410476694846	28.099889505652207
42	14.791531817795475	24.41892395504384	29.662828786065944	31.126715441094742
43	16.108964426050516	26.325768720292388	30.639624973030227	26.925641880626877
44	13.375046584155504	30.584050892781256	33.02669517290077	23.014207350162472
45	12.88370633348371	44.630890035240505	23.775899155927792	18.70950447534799
46	16.954998071252508	40.81785431745222	25.65626450646948	16.570883104825793
47	16.021026616715375	29.975351260877808	30.909322715414945	23.094299406991873
48	16.121713773872337	27.972722933788386	38.47524338178086	17.430319910558424
49	18.68989009408365	29.323499990192808	35.06920607522769	16.91740384049585
50	17.57088964295288	36.874056057901655	30.107094521703313	15.447959777442154
51	15.56597297138262	34.72824274758253	27.572916462350193	22.13286781868466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	182.0
1	403.0
2	624.0
3	2173.5
4	3723.0
5	2727.5
6	1732.0
7	1843.0
8	1954.0
9	2085.0
10	2216.0
11	2247.0
12	2278.0
13	2247.5
14	2217.0
15	2048.5
16	1880.0
17	1819.0
18	1758.0
19	1762.5
20	1767.0
21	1848.5
22	1930.0
23	2016.5
24	2103.0
25	2859.5
26	4662.0
27	5708.0
28	6838.5
29	7969.0
30	9581.5
31	11194.0
32	13236.5
33	15279.0
34	16353.5
35	17428.0
36	17845.5
37	18263.0
38	21082.0
39	23901.0
40	27636.0
41	31371.0
42	33483.5
43	35596.0
44	33238.0
45	30880.0
46	26808.5
47	22737.0
48	20455.0
49	18173.0
50	16147.5
51	14122.0
52	11625.0
53	9128.0
54	7057.0
55	4986.0
56	4031.0
57	3076.0
58	2697.5
59	2319.0
60	2021.5
61	1724.0
62	1548.0
63	1372.0
64	1134.0
65	896.0
66	738.0
67	580.0
68	508.5
69	437.0
70	371.5
71	306.0
72	256.0
73	206.0
74	159.0
75	92.5
76	73.0
77	59.5
78	46.0
79	34.5
80	23.0
81	15.5
82	8.0
83	5.0
84	2.0
85	2.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	305898.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.45720468914475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.59319268885461	21.383925360741163
2	7.798333129876039	4.594341904817946
3	3.664450831770411	3.2383343467430317
4	2.1973387785903737	2.589098326893278
5	1.4615632178805669	2.152678343761646
6	1.028754064521857	1.8182531432045976
7	0.8789355114361495	1.8123688288252948
8	0.6669700029963711	1.5717657519826873
9	0.6569820994573239	1.7417570562736597
>10	8.857050905015038	54.01898672106389
>50	0.17756272958306052	3.3050232430417985
>100	0.015536738838517795	0.7548267723228005
>500	0.002219534119788256	0.4854559362924896
>1k	0.001109767059894128	0.5331842640357244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1631	0.5331842640357244	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	755	0.2468142975763163	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	730	0.2386416387161734	No Hit
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	446	0.14580023406494974	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033671354503788845	0.0
2	0.0	0.0	0.0	0.11212887956116091	0.0
3	0.0	0.0	0.0	0.15201145479865838	0.0
4	0.0	0.0	0.0	0.25531386279086493	0.0
5	0.0	0.0	0.0	0.40307553498224896	0.0
6	0.0	0.0	0.0	0.5756820901084675	0.0
7	0.0	0.0	0.0	0.6793114044550798	0.0
8	0.0	0.0	0.0	1.0353124244029055	0.0
9	0.0	0.0	0.0	1.1680364042916267	0.0
10	0.0	0.0	0.0	1.4906929760900693	0.0
11	0.0	0.0	0.0	1.9441120896507986	0.0
12	0.0	0.0	0.0	2.2860561363591785	0.0
13	0.0	0.0	0.0	2.4455864373091685	0.0
14	0.0	0.0	0.0	2.5008336112037344	0.0
15	0.0	0.0	0.0	2.579944948969918	0.0
16	0.0	0.0	0.0	2.751897691387325	0.0
17	0.0	0.0	0.0	2.932349999019281	0.0
18	0.0	0.0	0.0	3.1566077581416025	0.0
19	0.0	0.0	0.0	3.290312457093541	0.0
20	0.0	0.0	0.0	3.4044027747811363	0.0
21	0.0	0.0	0.0	3.5776631426161662	0.0
22	0.0	0.0	0.0	3.7623652328553963	0.0
23	0.0	0.0	0.0	3.9742005505103006	0.0
24	0.0	0.0	0.0	4.118366252803222	0.0
25	0.0	0.0	0.0	4.229514413301166	0.0
26	0.0	0.0	0.0	4.349815951722469	0.0
27	0.0	0.0	0.0	4.458348861385168	0.0
28	0.0	0.0	0.0	4.5868230586666145	0.0
29	0.0	0.0	0.0	4.707451503442324	0.0
30	0.0	0.0	0.0	4.875481369606862	0.0
31	0.0	0.0	0.0	5.025531386279087	0.0
32	0.0	0.0	0.0	5.161524429711864	0.0
33	0.0	0.0	0.0	5.294575315954991	0.0
34	0.0	0.0	0.0	5.440048643665536	0.0
35	0.0	0.0	0.0	5.648941804130788	0.0
36	0.0	0.0	0.0	5.805203041536721	0.0
37	0.0	0.0	0.0	5.9572144963353795	0.0
38	0.0	0.0	0.0	6.089938476224101	0.0
39	0.0	0.0	0.0	6.246853526338845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	25	3.88407E-5	45.000004	1
ATGGGCG	25	3.88407E-5	45.000004	5
TTGGCAG	25	3.88407E-5	45.000004	2
TACTTAG	25	3.88407E-5	45.000004	2
GGACTCT	35	1.2080272E-7	45.000004	8
CGACCTA	25	3.88407E-5	45.000004	33
CGGGGGT	35	1.2080272E-7	45.000004	6
GCTCTAG	25	3.88407E-5	45.000004	2
GGGACGA	35	1.2080272E-7	45.000004	8
TATCTGG	25	3.88407E-5	45.000004	2
GGAACGC	25	3.88407E-5	45.000004	8
CTCGAGG	25	3.88407E-5	45.000004	3
TCGAGGA	25	3.88407E-5	45.000004	4
ACGACCT	25	3.88407E-5	45.000004	32
TACCGGG	35	1.2080272E-7	45.000004	3
TCACGGG	40	6.7866495E-9	45.0	3
CGTATGG	20	7.02516E-4	45.0	2
CCAGGTA	20	7.02516E-4	45.0	5
ATTACGG	20	7.02516E-4	45.0	2
GTAGGGT	55	1.8189894E-12	45.0	4
>>END_MODULE