##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700931
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64509916097305	31.0	31.0	33.0	30.0	34.0
2	31.98954105325631	31.0	31.0	34.0	30.0	34.0
3	32.00874551132708	33.0	31.0	34.0	30.0	34.0
4	35.791868243807166	37.0	35.0	37.0	35.0	37.0
5	35.71022540021771	37.0	35.0	37.0	35.0	37.0
6	35.85400845446984	37.0	35.0	37.0	35.0	37.0
7	4.902241447446325	0.0	0.0	0.0	0.0	35.0
8	19.610846146054318	17.0	17.0	17.0	17.0	35.0
9	31.774121846515563	32.0	32.0	32.0	28.0	38.0
10	35.21401250622387	37.0	34.0	37.0	32.0	37.0
11	36.80987286908412	37.0	35.0	39.0	33.0	39.0
12	37.14174005715256	39.0	37.0	39.0	34.0	39.0
13	36.793286357715665	39.0	35.0	39.0	33.0	39.0
14	38.23725302490545	40.0	37.0	41.0	34.0	41.0
15	38.438572412976455	40.0	38.0	41.0	34.0	41.0
16	38.53223926463518	40.0	38.0	41.0	34.0	41.0
17	38.537211223358646	40.0	38.0	41.0	34.0	41.0
18	38.43054309197339	40.0	37.0	41.0	34.0	41.0
19	38.36850132181342	40.0	37.0	41.0	34.0	41.0
20	38.27936273327902	40.0	37.0	41.0	34.0	41.0
21	38.147365432546145	40.0	37.0	41.0	34.0	41.0
22	38.046763518805705	40.0	37.0	40.0	34.0	41.0
23	38.02860481274191	40.0	37.0	40.0	34.0	41.0
24	37.882825841630634	40.0	36.0	40.0	34.0	41.0
25	37.77425452719312	39.0	36.0	40.0	33.0	41.0
26	37.515213337689445	39.0	36.0	40.0	33.0	41.0
27	37.2140053728541	39.0	35.0	40.0	33.0	41.0
28	37.23116826049925	39.0	36.0	40.0	33.0	41.0
29	37.19295194534127	38.0	36.0	40.0	33.0	41.0
30	36.88642105999021	38.0	35.0	40.0	32.0	41.0
31	36.62694188158321	38.0	35.0	40.0	32.0	40.0
32	36.295175987365376	38.0	35.0	40.0	31.0	40.0
33	36.18380411195967	38.0	35.0	40.0	31.0	40.0
34	36.0143965668518	38.0	35.0	40.0	31.0	40.0
35	35.870156976934965	38.0	34.0	40.0	30.0	40.0
36	35.61275788915029	38.0	34.0	39.0	30.0	40.0
37	35.33188288148192	37.0	34.0	39.0	30.0	40.0
38	35.05878895354892	37.0	34.0	39.0	29.0	40.0
39	34.93832060502389	37.0	34.0	39.0	29.0	40.0
40	34.50759917880647	36.0	33.0	38.0	29.0	40.0
41	34.166808145166925	36.0	33.0	38.0	27.0	40.0
42	34.03937192105928	36.0	33.0	38.0	26.0	40.0
43	34.09080494371058	36.0	33.0	38.0	26.0	40.0
44	33.8087985835981	36.0	33.0	38.0	26.0	40.0
45	33.20163040299259	35.0	31.0	38.0	24.0	39.0
46	33.08840813147086	35.0	31.0	38.0	24.0	39.0
47	33.06177640880486	35.0	31.0	38.0	24.0	39.0
48	32.6662895491853	34.0	31.0	38.0	24.0	39.0
49	32.56322376952938	34.0	31.0	38.0	24.0	39.0
50	32.39300302026876	34.0	31.0	37.0	24.0	39.0
51	31.785776346031206	34.0	30.0	37.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	7.0
15	7.0
16	26.0
17	62.0
18	142.0
19	330.0
20	641.0
21	1170.0
22	1993.0
23	3179.0
24	4597.0
25	5615.0
26	6757.0
27	7970.0
28	10170.0
29	13360.0
30	17758.0
31	25456.0
32	39331.0
33	56565.0
34	76308.0
35	115429.0
36	200840.0
37	94772.0
38	16743.0
39	1700.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.593596231298086	25.303774551275378	31.768604898342346	12.33402431908419
2	27.728121598274296	27.108231766036884	31.98203532159371	13.18161131409511
3	27.964236137365873	25.86246007096276	32.76627799312629	13.407025798545078
4	24.64136983526196	30.039048066072123	30.860241592967068	14.459340505698847
5	22.19704935293203	35.02855487915358	29.27149747978046	13.502898288133924
6	21.305092798007223	43.680048392780456	25.708236616728325	9.306622192483996
7	12.428184799930378	86.76845509757737	0.613327132057221	0.1900329704350357
8	91.25762735561703	3.609199764313463	3.5822356266165998	1.5509372534529076
9	86.36028367984866	6.777271942602053	4.838707376332335	2.023737001216953
10	46.93700235829204	34.06968731586989	11.093816652423705	7.8994936734143595
11	43.863661330430524	19.246544952356224	24.53023193438441	12.359561782828838
12	46.04461780118157	19.7965277609351	22.29777253395841	11.861081903924923
13	18.76803850878332	46.17929582227067	20.61443993773995	14.438225731206067
14	11.940119640877633	45.58180477108303	24.356320379609407	18.12175520842993
15	11.451769147034446	29.06634176545195	44.63007057756042	14.85181850995319
16	11.384002134304232	23.501172012651743	46.0819966587296	19.03282919431442
17	12.649176595128479	25.253270293366963	27.457624216934335	34.63992889457022
18	15.06310892227623	28.227172146759095	38.24199528912261	18.467723641842067
19	25.350141454722362	28.47912276672026	25.25726498043317	20.91347079812421
20	27.634674454404212	24.232627747952364	31.31963060558029	16.813067192063126
21	16.430290570683848	36.27047455455673	30.08898165439965	17.21025322035978
22	21.112206479667755	28.130728987589364	21.56289278117247	29.194171751570412
23	15.687849445951171	35.90738603371801	24.192823544685567	24.21194097564525
24	12.39394462507722	25.313475934150436	47.51780132423876	14.774778116533582
25	12.320328249142925	31.588843980363258	38.26082738529185	17.830000385201966
26	12.541034709550583	40.91786495389703	26.870833220388313	19.670267116164073
27	11.28384962285874	46.17501580041402	25.66101370891001	16.88012086781723
28	10.655542414303262	35.67184216420732	38.2027617554367	15.469853666052721
29	11.467319893113588	26.017539529568527	37.540642374213725	24.974498203104154
30	13.583790701224514	43.96980587247532	27.294983386381826	15.151420039918337
31	25.761451555145943	35.29263222770857	24.680746036343095	14.26517018080239
32	28.03713917632406	27.363606403483367	27.995194962128938	16.604059458063634
33	27.82599143139624	31.749059465197004	22.02955783094199	18.395391272464764
34	16.587082038032275	29.301029630591312	25.70495526663823	28.406933064738183
35	18.374561832762424	26.494048629608336	34.05898726122828	21.072402276400958
36	31.189232606347844	28.674577098173714	23.31499106188769	16.821199233590754
37	15.163261433721722	39.1172597588065	31.267271671534004	14.452207135937774
38	14.463763194950715	37.71198591587475	28.865466072980077	18.95878481619446
39	16.44955066903875	39.626867694537694	28.282669763500255	15.640911872923297
40	25.902549609020003	28.912546313403176	26.13538279802149	19.049521279555336
41	13.650559042188176	26.18602972332512	31.316777257675867	28.846633976810843
42	14.68718033586758	24.61483369975076	28.27040036751121	32.42758559687045
43	16.198598720844135	27.010219265519712	29.0102734791299	27.78090853450625
44	13.081173467859175	31.23659818156138	32.45340839540554	23.228819955173904
45	12.70453154447442	46.57976320065741	22.096611506696096	18.619093748172073
46	16.971142665968546	42.72517551656297	23.90163939103849	16.40204242642999
47	15.94579209651164	30.64124714130207	29.62502728513934	23.78793347704696
48	16.013131107056186	28.558160503672976	38.107745270219176	17.32096311905166
49	18.75477044102772	30.277445283487246	34.42307445383355	16.54470982165149
50	17.702455733873947	38.56214092399965	28.7342120693763	15.0011912727501
51	15.564014146898911	36.2510717888066	26.26806347557748	21.916850588717008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	404.0
1	677.0
2	950.0
3	1753.0
4	2556.0
5	2606.0
6	2656.0
7	2614.0
8	2572.0
9	2819.0
10	3066.0
11	3129.5
12	3193.0
13	3008.5
14	2824.0
15	2953.5
16	3083.0
17	2772.5
18	2462.0
19	3026.5
20	3591.0
21	3823.5
22	4056.0
23	4544.5
24	5033.0
25	8156.5
26	13281.5
27	15283.0
28	19076.0
29	22869.0
30	27222.5
31	31576.0
32	37231.0
33	42886.0
34	41607.5
35	40329.0
36	45270.5
37	50212.0
38	57624.5
39	65037.0
40	75676.5
41	86316.0
42	85127.0
43	83938.0
44	72909.5
45	61881.0
46	54183.0
47	46485.0
48	41625.0
49	36765.0
50	30949.0
51	25133.0
52	19702.5
53	14272.0
54	11230.0
55	8188.0
56	7099.5
57	6011.0
58	5268.5
59	4526.0
60	4001.5
61	3477.0
62	3077.5
63	2678.0
64	2268.5
65	1859.0
66	1542.5
67	1226.0
68	1020.0
69	814.0
70	724.0
71	634.0
72	472.5
73	311.0
74	252.0
75	180.0
76	167.0
77	119.0
78	71.0
79	53.5
80	36.0
81	22.5
82	9.0
83	12.5
84	16.0
85	10.5
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	700931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.592625137224662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8900096261663	15.421818947547894
2	7.818108166354943	3.2199074150404448
3	3.055185968554174	1.8874289812483434
4	1.7715386726285574	1.4592252720614576
5	1.2530988017761726	1.2902296942441058
6	0.9936529236195568	1.227715330356292
7	0.8644011690711421	1.246020247000255
8	0.7260756637890241	1.196144317253515
9	0.6392349519922301	1.184717316688902
>10	5.451777925096126	21.937648156839227
>50	1.6652004559991802	25.545100120719606
>100	0.8682232243317874	23.5697803801956
>500	0.00209547037248219	0.2603457897290072
>1k	0.0013969802483214599	0.5539180310753627
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2741	0.3910513303021268	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1110	0.15836080869586305	No Hit
AAGGAAAGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	751	0.10714321381134519	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0028533479044299654	0.0
2	0.0	0.0	0.0	0.014266739522149826	0.0
3	0.0	0.0	0.0	0.01797609179790878	0.0
4	0.0	0.0	0.0	0.030245487786957632	0.0
5	0.0	0.0	0.0	0.05321493841761885	0.0
6	0.0	0.0	0.0	0.07746839560527356	0.0
7	0.0	0.0	0.0	0.0904511285704299	0.0
8	0.0	0.0	0.0	0.3459684334121333	0.0
9	0.0	0.0	0.0	0.4418409230009801	0.0
10	0.0	0.0	0.0	0.6675407422413904	0.0
11	0.0	0.0	0.0	0.9642889243021068	0.0
12	0.0	0.0	0.0	1.2004034633936864	0.0
13	0.0	0.0	0.0	1.2948492790303183	0.0
14	0.0	0.0	0.0	1.3336548105305657	0.0
15	0.0	0.0	0.0	1.3934324491283736	0.0
16	0.0	0.0	0.0	1.5085650370721226	0.0
17	0.0	0.0	0.0	1.6386777015141292	0.0
18	0.0	0.0	0.0	1.8045998821567315	0.0
19	0.0	0.0	0.0	1.8879176409660865	0.0
20	0.0	0.0	0.0	1.9720914041467705	0.0
21	0.0	0.0	0.0	2.1040587447266565	0.0
22	0.0	0.0	0.0	2.236454087492207	0.0
23	0.0	0.0	0.0	2.39581356795462	0.0
24	0.0	0.0	0.0	2.4992474294902065	0.0
25	0.0	0.0	0.0	2.5834211926708908	0.0
26	0.0	0.0	0.0	2.6678802906420174	0.0
27	0.0	0.0	0.0	2.745063351456848	0.0
28	0.0	0.0	0.0	2.8373691561651575	0.0
29	0.0	0.0	0.0	2.934668319706219	0.0
30	0.0	0.0	0.0	3.06235563842946	0.0
31	0.0	0.0	0.0	3.175062880654444	0.0
32	0.0	0.0	0.0	3.276499398656929	0.0
33	0.0	0.0	0.0	3.378934588425965	0.0
34	0.0	0.0	0.0	3.5021992178973393	0.0
35	0.0	0.0	0.0	3.654710663389121	0.0
36	0.0	0.0	0.0	3.772125929656414	0.0
37	0.0	0.0	0.0	3.8882571893667137	0.0
38	0.0	0.0	0.0	4.0033897773104625	0.0
39	0.0	0.0	0.0	4.127225076362723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.0314E-4	45.000004	1
GACAGTA	20	7.0314E-4	45.000004	9
AGGTAGC	40	6.8084773E-9	45.000004	7
GGTACGC	20	7.0314E-4	45.000004	8
CCGGGAT	20	7.0314E-4	45.000004	18
CTCGTCT	20	7.0314E-4	45.000004	40
CCAGGTA	40	6.8084773E-9	45.000004	5
GAGTCGA	20	7.0314E-4	45.000004	9
CGTCTAT	20	7.0314E-4	45.000004	42
ACCCTGG	20	7.0314E-4	45.000004	27
GGCCCCA	20	7.0314E-4	45.000004	9
GCCGATC	30	2.1642845E-6	45.000004	9
GGGACGT	20	7.0314E-4	45.000004	9
CGGATCA	20	7.0314E-4	45.000004	33
CACTAGG	75	0.0	45.000004	3
CTTGCGA	20	7.0314E-4	45.000004	1
AGCGCGA	20	7.0314E-4	45.000004	1
GTCGTTT	20	7.0314E-4	45.000004	9
TCGTCGA	20	7.0314E-4	45.000004	1
CGAATCT	20	7.0314E-4	45.000004	13
>>END_MODULE