FastQCFastQC Report
Sat 18 Jun 2016
SRR3549325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153591
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67210.5826155023747585No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT14050.1217935992912566No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC13460.11667913497938177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC207.0332957E-445.00000413
TTAGGTA351.211938E-745.00000432
TGTCACG406.8157533E-945.0000041
AACCGTG207.0332957E-445.00000435
AGCGTGT207.0332957E-445.00000427
AATCCGG207.0332957E-445.0000042
GCGACTT207.0332957E-445.00000445
TCCGCTA406.8157533E-945.00000440
CGAGTAA207.0332957E-445.00000438
ACGTTCG406.8157533E-945.0000041
GAGTACG650.045.0000041
GCACGTA207.0332957E-445.00000444
CGCGCAC207.0332957E-445.00000438
CACGATT207.0332957E-445.00000418
TAACGCG207.0332957E-445.0000041
TCGATAG207.0332957E-445.0000041
AACGGTC207.0332957E-445.00000422
TGTTGCG351.211938E-745.0000041
ATCCGAT207.0332957E-445.00000429
AGCGGTA207.0332957E-445.0000044