##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549325_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1153591
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.524543794117672	31.0	31.0	33.0	30.0	34.0
2	31.91386721983788	31.0	31.0	34.0	30.0	34.0
3	31.97934103161346	33.0	31.0	34.0	30.0	34.0
4	35.688106096528145	37.0	35.0	37.0	33.0	37.0
5	35.58872685379827	37.0	35.0	37.0	33.0	37.0
6	35.65610862081969	37.0	35.0	37.0	33.0	37.0
7	36.0563709321588	37.0	35.0	37.0	35.0	37.0
8	36.09806335174252	37.0	35.0	37.0	35.0	37.0
9	37.929077116586384	39.0	38.0	39.0	35.0	39.0
10	37.309351407908004	39.0	37.0	39.0	34.0	39.0
11	36.92453824622418	39.0	37.0	39.0	33.0	39.0
12	36.145676413911	37.0	35.0	39.0	32.0	39.0
13	35.80362017387445	38.0	35.0	39.0	31.0	39.0
14	36.79223919049299	39.0	35.0	41.0	31.0	41.0
15	37.100287710289	39.0	35.0	41.0	32.0	41.0
16	37.28007500058513	39.0	35.0	41.0	33.0	41.0
17	37.220835634119894	39.0	35.0	41.0	32.0	41.0
18	37.169477743845086	38.0	35.0	41.0	32.0	41.0
19	37.11817359878848	38.0	35.0	41.0	32.0	41.0
20	36.9600551668659	38.0	35.0	41.0	32.0	41.0
21	36.81849026214664	38.0	35.0	41.0	32.0	41.0
22	36.71456608104606	38.0	35.0	41.0	31.0	41.0
23	36.669349015378934	38.0	35.0	41.0	31.0	41.0
24	36.68949046932578	38.0	35.0	41.0	32.0	41.0
25	36.67042738717622	38.0	35.0	41.0	32.0	41.0
26	36.50201761282812	38.0	35.0	40.0	31.0	41.0
27	36.448157969332286	38.0	35.0	40.0	31.0	41.0
28	36.427577885056316	38.0	35.0	40.0	31.0	41.0
29	36.406793222207874	38.0	35.0	40.0	31.0	41.0
30	36.354219996515226	38.0	35.0	40.0	31.0	41.0
31	36.223005380589825	38.0	35.0	40.0	31.0	41.0
32	36.06216154598987	38.0	35.0	40.0	30.0	41.0
33	35.976053037861774	38.0	35.0	40.0	30.0	41.0
34	35.88366500778872	38.0	35.0	40.0	30.0	41.0
35	35.78620932375512	38.0	35.0	40.0	30.0	41.0
36	35.61411800196083	38.0	35.0	40.0	29.0	41.0
37	35.52512372235914	38.0	34.0	40.0	29.0	41.0
38	35.444677533025136	37.0	34.0	40.0	29.0	41.0
39	35.480990229639445	37.0	34.0	40.0	29.0	41.0
40	35.31898307112313	37.0	34.0	40.0	28.0	41.0
41	35.28979595021112	37.0	34.0	40.0	28.0	41.0
42	35.22310333558428	37.0	34.0	40.0	27.0	41.0
43	35.081440475870565	37.0	34.0	40.0	27.0	41.0
44	34.89030340909387	37.0	34.0	40.0	26.0	41.0
45	34.87154892851973	37.0	34.0	40.0	26.0	41.0
46	34.757560521883406	36.0	34.0	40.0	26.0	41.0
47	34.67072645330971	36.0	34.0	40.0	26.0	41.0
48	34.554242361460865	36.0	34.0	40.0	26.0	41.0
49	34.47368434739869	36.0	34.0	40.0	24.0	41.0
50	34.30086313086701	36.0	34.0	40.0	24.0	41.0
51	33.93830569066506	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	3.0
12	3.0
13	7.0
14	13.0
15	21.0
16	61.0
17	157.0
18	400.0
19	733.0
20	1379.0
21	2298.0
22	3632.0
23	5378.0
24	7786.0
25	11251.0
26	14770.0
27	17625.0
28	19950.0
29	23965.0
30	28323.0
31	34287.0
32	43350.0
33	56315.0
34	101823.0
35	172469.0
36	76608.0
37	93167.0
38	146766.0
39	290989.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.830093161267726	24.301420520791165	29.890836526984	12.977649790957107
2	29.296258379269602	27.94239899583128	30.610849079093022	12.150493545806096
3	29.724659779765965	25.2209838668991	32.16148530978484	12.892871043550096
4	26.02993608653327	27.96138319387027	30.706723613481728	15.301957106114733
5	23.12986145002865	32.15229661119062	29.96772686333371	14.750115075447018
6	21.238549884664497	41.8319837793464	26.45426325274729	10.475203083241807
7	88.722606192316	4.406154347598065	5.326931295407125	1.544308164678816
8	89.78494110997745	3.2143107912596407	5.022924069275852	1.977824029487054
9	85.4556770987291	5.433901616777524	6.559690566240548	2.55073071825283
10	48.89263179064331	28.4359014590093	13.147640714950098	9.523826035397294
11	40.77146926423663	22.906298679514663	22.263263149591147	14.05896890665756
12	38.056122143810065	23.029132508835453	23.772030121594224	15.142715225760256
13	22.02210315441088	38.69794407203246	24.216815145055744	15.063137628500916
14	14.858038941011156	41.198657063031874	26.703138287313266	17.240165708643705
15	13.815381708075044	28.213812347703822	41.83692487198669	16.13388107223444
16	15.861340804496567	22.38150262961483	43.05434074988449	18.70281581600411
17	16.061845142689222	24.523336260425054	27.614986594035496	31.79983200285023
18	20.24391660475853	27.363597670231478	32.233347867658466	20.15913785735152
19	26.56643472426536	29.335960492063478	24.436563738794774	19.66104104487639
20	28.66067783122441	26.43727282893157	26.009738286793155	18.892311053050864
21	21.336071449933293	30.309355742199788	27.415262428364993	20.939310379501922
22	21.640772162750924	26.64635906486788	26.224025672877126	25.48884309950407
23	17.90071177739771	32.176395273541495	26.7623447131609	23.1605482358999
24	19.044618066541783	26.62304057503916	35.787120391889324	18.545220966529733
25	17.41449092442642	29.40721624908655	32.35635506865085	20.821937757836185
26	15.774048167851518	37.56574037072065	26.040078329321226	20.620133132106613
27	17.628171509659836	36.337315391676945	28.480197921100288	17.55431517756293
28	15.119656793438924	30.140405048236335	37.71128588901959	17.02865226930515
29	16.069906925418106	26.725503232948245	36.673829806231154	20.530760035402494
30	19.01349785149156	31.329214600321954	31.09767673291487	18.55961081527162
31	28.653396221017672	28.870544239682864	24.240480378227637	18.23557916107182
32	28.021889907254828	29.794181820073145	25.256698431246427	16.9272298414256
33	26.680860027514086	30.11197209409574	24.025672877128894	19.18149500126128
34	18.956111828195606	29.656264655324115	28.16734873971798	23.220274776762302
35	19.814474974232635	28.23132288653431	30.135377269760255	21.818824869472802
36	27.577798370479663	27.229407996421607	27.646540238264688	17.546253394834043
37	20.035697227180172	32.83590111226596	29.657825000368415	17.470576660185454
38	18.66510747743351	34.5890354553737	25.836193243532584	20.909663823660203
39	19.542281449837944	33.1230912862531	27.51122364858949	19.823403615319467
40	23.58712923384458	28.506983844360782	26.13335228863609	21.772534633158546
41	17.830496250404174	25.85942504752551	29.238352240958882	27.071726461111435
42	21.65984304662571	27.58247940561256	25.278803319374028	25.478874228387703
43	21.327749609697026	28.199422498961933	26.373645425458413	24.09918246588262
44	19.511681349802487	32.3743857224961	27.957569017095313	20.1563639106061
45	18.701948957646167	37.215356222439326	23.404308806154	20.678386013760512
46	22.966198592048656	33.34578719840914	24.764149512262147	18.923864697280056
47	20.959074750063063	29.819754141632522	27.03185097664597	22.189320131658448
48	21.37421321768287	27.06929925770919	31.746000098821852	19.81048742578609
49	20.566041170570852	26.515723510325586	30.420920412867297	22.497314906236266
50	18.58865057026277	32.23430141185221	28.61629468329763	20.56075333458739
51	17.527182510959257	34.64382090359582	25.522737261299717	22.306259324145213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	792.0
1	1249.5
2	1707.0
3	5421.0
4	9135.0
5	6476.0
6	3817.0
7	3863.5
8	3910.0
9	4287.0
10	4664.0
11	4994.0
12	5324.0
13	5228.0
14	5132.0
15	4691.0
16	4250.0
17	3953.5
18	3657.0
19	3686.5
20	3716.0
21	3775.0
22	3834.0
23	3803.5
24	3773.0
25	5071.0
26	8346.5
27	10324.0
28	13934.5
29	17545.0
30	21332.0
31	25119.0
32	28052.5
33	30986.0
34	34740.5
35	38495.0
36	40886.0
37	43277.0
38	49132.0
39	54987.0
40	72748.5
41	90510.0
42	104891.0
43	119272.0
44	120937.0
45	122602.0
46	115849.0
47	109096.0
48	107740.0
49	106384.0
50	97828.5
51	89273.0
52	77724.0
53	66175.0
54	57872.5
55	49570.0
56	42303.5
57	35037.0
58	30857.0
59	26677.0
60	24692.0
61	22707.0
62	18666.5
63	14626.0
64	11358.0
65	8090.0
66	6533.5
67	4977.0
68	3714.5
69	2452.0
70	2263.5
71	2075.0
72	1877.5
73	1680.0
74	1197.0
75	520.0
76	326.0
77	245.0
78	164.0
79	139.0
80	114.0
81	100.0
82	86.0
83	75.5
84	65.0
85	51.0
86	37.0
87	33.0
88	29.0
89	25.5
90	22.0
91	16.0
92	10.0
93	8.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1153591.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.14111888643795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71440463757371	16.852517054045666
2	8.779326432779404	3.712095677164731
3	2.944625417716102	1.867580280958894
4	1.355383740800383	1.1461731520402358
5	0.7976105627193406	0.8431189865764126
6	0.5622102516121384	0.7131452261103847
7	0.37861720736631693	0.5603073975367706
8	0.30374596320749403	0.5137223615556191
9	0.24456116905713537	0.46532670750388355
>10	2.5909779571314675	12.914858629784371
>50	1.0246875006761194	16.009559582167725
>100	1.2964595860391104	42.647027548272504
>500	0.005336914065392159	0.743104770823787
>1k	0.0016421274047360489	0.42814013483692376
>5k	4.105318511840122E-4	0.5833224906221295
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6721	0.5826155023747585	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1405	0.1217935992912566	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1346	0.11667913497938177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08919972503252886	0.0
2	0.0	0.0	0.0	0.35263798001198	0.0
3	0.0	0.0	0.0	0.5034713342943903	0.0
4	0.0	0.0	0.0	0.7450647586536303	0.0
5	0.0	0.0	0.0	1.2635327425404672	0.0
6	0.0	0.0	0.0	1.7025098150037579	0.0
7	0.0	0.0	0.0	1.9999289176146484	0.0
8	0.0	0.0	0.0	2.7014773866994455	0.0
9	0.0	0.0	0.0	2.9213993521100634	0.0
10	0.0	0.0	0.0	3.343646058265018	0.0
11	0.0	0.0	0.0	3.93406328586128	0.0
12	0.0	0.0	0.0	4.373127044160365	0.0
13	0.0	0.0	0.0	4.543204653989152	0.0
14	0.0	0.0	0.0	4.612033207609977	0.0
15	0.0	0.0	0.0	4.723251134934305	0.0
16	0.0	0.0	0.0	5.002379526192558	0.0
17	0.0	0.0	0.0	5.335253135643395	0.0
18	0.0	0.0	0.0	5.740856161325808	0.0
19	0.0	0.0	0.0	5.940927070339487	0.0
20	0.0	0.0	0.0	6.151313593812712	0.0
21	0.0	0.0	0.0	6.429835184220404	0.0
22	0.0	0.0	0.0	6.713991353954738	0.0
23	0.0	0.0	0.0	7.021379327681995	0.0
24	0.0	0.0	0.0	7.245288841539159	0.0
25	0.0	0.0	0.0	7.42221463239571	0.0
26	0.0	0.0	0.0	7.5875245212558005	0.0
27	0.0	0.0	0.0	7.7760662141088135	0.0
28	0.0	0.0	0.0	7.9689421987515505	0.0
29	0.0	0.0	0.0	8.181062438940664	0.0
30	0.0	0.0	0.0	8.426556725910656	0.0
31	8.668583579448869E-5	0.0	0.0	8.652026584812122	0.0
32	8.668583579448869E-5	0.0	0.0	8.855651613093375	0.0
33	8.668583579448869E-5	0.0	0.0	9.067858539118284	0.0
34	8.668583579448869E-5	0.0	0.0	9.286740274499367	0.0
35	8.668583579448869E-5	0.0	0.0	9.561187630624719	0.0
36	8.668583579448869E-5	0.0	0.0	9.782409883572255	0.0
37	8.668583579448869E-5	0.0	0.0	10.016548326053169	0.0
38	8.668583579448869E-5	0.0	0.0	10.227628336212748	0.0
39	8.668583579448869E-5	0.0	0.0	10.462980380394784	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.0332957E-4	45.000004	13
TTAGGTA	35	1.211938E-7	45.000004	32
TGTCACG	40	6.8157533E-9	45.000004	1
AACCGTG	20	7.0332957E-4	45.000004	35
AGCGTGT	20	7.0332957E-4	45.000004	27
AATCCGG	20	7.0332957E-4	45.000004	2
GCGACTT	20	7.0332957E-4	45.000004	45
TCCGCTA	40	6.8157533E-9	45.000004	40
CGAGTAA	20	7.0332957E-4	45.000004	38
ACGTTCG	40	6.8157533E-9	45.000004	1
GAGTACG	65	0.0	45.000004	1
GCACGTA	20	7.0332957E-4	45.000004	44
CGCGCAC	20	7.0332957E-4	45.000004	38
CACGATT	20	7.0332957E-4	45.000004	18
TAACGCG	20	7.0332957E-4	45.000004	1
TCGATAG	20	7.0332957E-4	45.000004	1
AACGGTC	20	7.0332957E-4	45.000004	22
TGTTGCG	35	1.211938E-7	45.000004	1
ATCCGAT	20	7.0332957E-4	45.000004	29
AGCGGTA	20	7.0332957E-4	45.000004	4
>>END_MODULE