FastQCFastQC Report
Sat 18 Jun 2016
SRR3549323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299274
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48430.3727466261927815No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24420.18795111731628586No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG17060.13130409751907604No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG15910.122453000675762No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT14050.10813731360744538No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13930.10721372089336045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGT302.1657215E-645.00000444
CGTAATT302.1657215E-645.00000436
TATAGCG600.045.0000041
AGCGTAT207.0336263E-445.020
ACGTTAT253.8910875E-545.016
TAACGCG253.8910875E-545.01
CGACACG253.8910875E-545.034
TACGCTA351.2120836E-745.026
GTTAACG551.8189894E-1245.01
CGCTAAC207.0336263E-445.011
TTGACGA253.8910875E-545.022
CAGTCGT207.0336263E-445.018
ACGACAC253.8910875E-545.033
TAGTACG1100.045.01
GTACGAC351.2120836E-745.012
AGTACGG3050.043.524592
CTACGAA2150.042.90697511
GCTACGA2150.042.90697510
CTACGGG1800.042.53
CACGACG4450.042.47191226