##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2378805
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55285363869674	31.0	31.0	33.0	30.0	34.0
2	31.949709623109083	31.0	31.0	34.0	30.0	34.0
3	32.03379680133512	33.0	31.0	34.0	30.0	34.0
4	35.74809452645341	37.0	35.0	37.0	35.0	37.0
5	35.64267941256219	37.0	35.0	37.0	33.0	37.0
6	35.715615613722015	37.0	35.0	37.0	33.0	37.0
7	36.06209168048663	37.0	35.0	37.0	35.0	37.0
8	36.08877860942784	37.0	35.0	37.0	35.0	37.0
9	37.90632649586662	39.0	38.0	39.0	35.0	39.0
10	37.3728796601655	39.0	37.0	39.0	35.0	39.0
11	36.9923272399377	39.0	37.0	39.0	33.0	39.0
12	35.97876202547077	37.0	35.0	39.0	32.0	39.0
13	35.53129575564201	37.0	35.0	39.0	31.0	39.0
14	36.47384253858555	38.0	35.0	41.0	31.0	41.0
15	36.77801879515135	38.0	35.0	41.0	32.0	41.0
16	36.982497934887476	38.0	35.0	41.0	32.0	41.0
17	36.92868057701241	38.0	35.0	41.0	32.0	41.0
18	36.87878157310078	38.0	35.0	41.0	32.0	41.0
19	36.820646501079324	38.0	35.0	41.0	32.0	41.0
20	36.69813624908305	38.0	35.0	41.0	32.0	41.0
21	36.52460752352547	38.0	35.0	40.0	31.0	41.0
22	36.41223303297244	38.0	35.0	40.0	31.0	41.0
23	36.383434119232135	37.0	35.0	40.0	31.0	41.0
24	36.37377548811273	37.0	35.0	40.0	31.0	41.0
25	36.327989894085476	37.0	35.0	40.0	31.0	41.0
26	36.19698504080831	37.0	35.0	40.0	31.0	41.0
27	36.136001479734574	37.0	35.0	40.0	31.0	41.0
28	36.10736104893003	37.0	35.0	40.0	31.0	41.0
29	36.10807191005568	37.0	35.0	40.0	31.0	41.0
30	36.06833473109398	37.0	35.0	40.0	31.0	41.0
31	35.954493537721675	37.0	35.0	40.0	30.0	41.0
32	35.795396007659306	37.0	35.0	40.0	30.0	41.0
33	35.707646065986914	37.0	35.0	40.0	30.0	41.0
34	35.633729120293594	37.0	35.0	40.0	30.0	41.0
35	35.51503296823405	37.0	34.0	40.0	30.0	41.0
36	35.324087094150215	36.0	34.0	40.0	29.0	41.0
37	35.1971628611845	36.0	34.0	40.0	28.0	41.0
38	35.12476474532381	36.0	34.0	40.0	28.0	41.0
39	35.129087924399016	36.0	34.0	40.0	28.0	41.0
40	34.966779538465744	36.0	34.0	40.0	27.0	41.0
41	34.98177151973365	36.0	34.0	40.0	27.0	41.0
42	34.940293130374286	36.0	34.0	40.0	27.0	41.0
43	34.801983348782265	35.0	34.0	40.0	27.0	41.0
44	34.632174978613214	35.0	34.0	40.0	26.0	41.0
45	34.609854527798625	35.0	34.0	40.0	26.0	41.0
46	34.56170640300487	35.0	34.0	40.0	26.0	41.0
47	34.491597671940326	35.0	34.0	40.0	26.0	41.0
48	34.39920842607948	35.0	34.0	40.0	26.0	41.0
49	34.347042737845264	35.0	34.0	40.0	26.0	41.0
50	34.14245556067017	35.0	33.0	39.0	24.0	41.0
51	33.748756203219685	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	2.0
12	2.0
13	13.0
14	22.0
15	68.0
16	158.0
17	437.0
18	940.0
19	1930.0
20	3416.0
21	5658.0
22	8396.0
23	12209.0
24	17266.0
25	24240.0
26	31429.0
27	37922.0
28	42383.0
29	49484.0
30	58860.0
31	72559.0
32	92144.0
33	122512.0
34	234604.0
35	404135.0
36	145550.0
37	183521.0
38	286958.0
39	541855.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.49547356761063	23.90380884519748	30.37306546774536	12.227652119446528
2	29.97933836527164	25.97468056440103	30.81450560260299	13.23147546772434
3	29.026549044583312	25.526808628702224	31.31971725299047	14.126925073723992
4	26.43718169416997	27.79063437314114	30.89450375293477	14.87768017975412
5	23.68294164506969	33.94935692501066	28.21492303908895	14.15277839083069
6	22.023999445099534	42.02984271514479	25.05035091148707	10.895806928268605
7	88.72686075571558	4.331754809662835	5.216358633851871	1.7250258007697143
8	90.26607897662903	3.203078856820967	4.674363808719083	1.8564783578309276
9	85.64560777365105	5.555562561874554	6.335912359356905	2.4629173051174855
10	52.3695300791784	27.97080887252213	10.734633566013187	8.925027482286275
11	47.03664234773342	18.797379356441574	20.671009183182314	13.494969112642693
12	42.78799649403797	19.8645538411093	23.191728620042415	14.155721044810315
13	23.37791454112464	39.02778916304615	22.791485640899527	14.802810654929683
14	15.577191068624794	42.83827383917555	26.439451741525684	15.145083350673975
15	14.1472293861834	25.255033514726932	46.3923692778517	14.205367821237974
16	16.8269362137712	19.461368207986784	45.96093416652479	17.750761411717228
17	17.306799002019922	19.749369956764003	27.591500774548567	35.35233026666751
18	22.707241661254287	23.485069183896957	33.21209598937281	20.595593165475943
19	27.751959492266074	25.3531920439044	26.3304474305376	20.564401033291926
20	31.137693085393714	23.393636720958632	25.033409632147237	20.435260561500417
21	23.08823127578763	27.413596322523283	28.278652516704817	21.219519884984265
22	23.05645061280769	23.430672123187904	26.711310931328963	26.801566332675442
23	19.744283369170653	29.743043250707814	24.963248353690194	25.549425026431337
24	20.510676579206784	24.38354551970422	36.87334607082127	18.232431830267718
25	17.481844875893568	25.163390862218638	34.847286767936005	22.507477493951793
26	17.17690184777651	35.00854420601941	26.651743207198574	21.162810739005508
27	18.090259605137874	34.17753872217353	28.518941233098133	19.213260439590467
28	14.62717625026011	29.41619846939955	38.516313863473464	17.440311416866873
29	16.709818585382155	24.65019200817217	37.00597568947433	21.63401371697134
30	18.4087808794752	30.145388125550433	32.115200699510886	19.33063029546348
31	28.670109571822827	27.092847038744246	24.40469059044352	19.832352798989408
32	28.840909616383016	28.107558206746663	26.119164874800582	16.932367302069736
33	26.921500501302127	29.539916050285754	24.51314840854967	19.02543503986245
34	20.148435874315044	29.1957936863257	27.53647314512959	23.119297294229664
35	19.995712132772546	27.82422266642285	30.207646276176487	21.972418924628123
36	29.512255102877283	25.387999436691956	26.90985599912561	18.18988946130515
37	21.526354619231082	31.505819098244707	28.607346966228842	18.36047931629537
38	20.318184971025367	32.379829368107096	24.43352859944384	22.868457061423697
39	20.993019604381193	30.709663045100378	27.715008165864795	20.58230918465364
40	23.551657239664454	26.91221012230931	25.92280577853166	23.61332685949458
41	17.60909364155532	25.34818953213904	28.457818106149936	28.58489872015571
42	23.37093624740153	25.93835980670967	24.811617597911557	25.879086347977243
43	23.633294868642025	25.965600374978195	25.64073137562768	24.760373380752103
44	19.98331094814413	31.22130649632904	27.79702413606832	20.99835841945851
45	17.95914335138862	36.804740195182035	22.966531514773177	22.269584938656173
46	23.142712412324677	32.65681718341772	25.255411855952886	18.945058548304715
47	22.555316640077685	28.171245646448533	26.424696433713567	22.848741279760215
48	23.52794785617148	24.840623758567855	29.563667471692717	22.067760913567945
49	21.257269931751445	23.933697802047668	31.037642850086495	23.771389416114395
50	20.114132936495423	30.628698022746715	28.425575026116057	20.831594014641805
51	18.923451060511475	33.01884769873949	24.339657937493826	23.718043303255207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1619.0
1	2592.5
2	3566.0
3	8435.5
4	13305.0
5	10132.5
6	6960.0
7	7023.5
8	7087.0
9	7570.0
10	8053.0
11	7840.5
12	7628.0
13	7674.5
14	7721.0
15	7528.0
16	7335.0
17	6942.5
18	6550.0
19	6380.5
20	6211.0
21	6605.5
22	7000.0
23	7577.0
24	8154.0
25	10888.5
26	16515.0
27	19407.0
28	22429.0
29	25451.0
30	31156.5
31	36862.0
32	44087.0
33	51312.0
34	52515.0
35	53718.0
36	61734.0
37	69750.0
38	84903.0
39	100056.0
40	135888.0
41	171720.0
42	211458.5
43	251197.0
44	257798.5
45	264400.0
46	254103.5
47	243807.0
48	224307.0
49	204807.0
50	183326.0
51	161845.0
52	148910.5
53	135976.0
54	121909.0
55	107842.0
56	98205.0
57	88568.0
58	82111.0
59	75654.0
60	71580.0
61	67506.0
62	60361.0
63	53216.0
64	43995.5
65	34775.0
66	26964.0
67	19153.0
68	16738.5
69	14324.0
70	11975.0
71	9626.0
72	7718.5
73	5811.0
74	4694.0
75	2648.0
76	1719.0
77	1367.5
78	1016.0
79	712.5
80	409.0
81	281.5
82	154.0
83	163.0
84	172.0
85	131.0
86	90.0
87	60.0
88	30.0
89	28.0
90	26.0
91	17.0
92	8.0
93	7.5
94	7.0
95	4.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2378805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.082794786536923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.99263942401954	14.645732779245254
2	8.555447036122574	3.0941278612258016
3	2.6294001447883146	1.4264070968969271
4	1.24375477980218	0.8996224979174896
5	0.7009611156219918	0.633766800356723
6	0.4386166880849264	0.4758849336354119
7	0.2949031646594236	0.3732871385905666
8	0.22191626586154492	0.321029303629511
9	0.1834994124902569	0.2986363997560265
>10	1.8867664290825208	8.196997316058841
>50	1.0973834615237394	14.671305400872656
>100	1.7425993701298776	52.79580045096589
>500	0.010482150992126333	1.2317158328215254
>1k	0.0011646834435695924	0.2741264838247208
>5k	4.65873377427837E-4	0.6615597042026837
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8058	0.3387415109687427	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7648	0.3215059662309437	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04552706085618619	0.0
2	0.0	0.0	0.0	0.1565912296300033	0.0
3	0.0	0.0	0.0	0.2249028398712799	0.0
4	0.0	0.0	0.0	0.3559350177925471	0.0
5	0.0	0.0	0.0	0.6244311744762602	0.0
6	0.0	0.0	0.0	0.8949451510317155	0.0
7	0.0	0.0	0.0	1.068183394603593	0.0
8	0.0	0.0	0.0	1.5525022017357455	0.0
9	0.0	0.0	0.0	1.7322142840627963	0.0
10	0.0	0.0	0.0	2.0866359369515366	0.0
11	0.0	0.0	0.0	2.425209296264301	0.0
12	0.0	0.0	0.0	2.720147300850637	0.0
13	0.0	0.0	0.0	2.8413426068971606	0.0
14	0.0	0.0	0.0	2.886743554011363	0.0
15	0.0	0.0	0.0	2.9639251641055067	0.0
16	0.0	0.0	0.0	3.135355777375615	0.0
17	0.0	0.0	0.0	3.331756911558535	0.0
18	0.0	0.0	0.0	3.6322439207921624	0.0
19	0.0	0.0	0.0	3.761762733809623	0.0
20	0.0	0.0	0.0	3.9068776129190916	0.0
21	0.0	0.0	0.0	4.086001164450217	0.0
22	0.0	0.0	0.0	4.2656712088632736	0.0
23	0.0	0.0	0.0	4.471320684125012	0.0
24	0.0	0.0	0.0	4.622026605795767	0.0
25	0.0	0.0	0.0	4.746963286187813	0.0
26	0.0	0.0	0.0	4.873665558967633	0.0
27	0.0	0.0	0.0	4.9941041825622525	0.0
28	0.0	0.0	0.0	5.123622995579713	0.0
29	0.0	0.0	0.0	5.264534083289719	0.0
30	0.0	0.0	0.0	5.442438535315	0.0
31	0.0	0.0	0.0	5.61605512011283	0.0
32	0.0	0.0	0.0	5.766172510987659	0.0
33	0.0	0.0	0.0	5.915070802356645	0.0
34	0.0	0.0	0.0	6.077673453687881	0.0
35	0.0	0.0	0.0	6.282271981099754	0.0
36	0.0	0.0	0.0	6.46875216757994	0.0
37	0.0	0.0	0.0	6.6400987050220595	0.0
38	0.0	0.0	0.0	6.817708891649379	0.0
39	0.0	0.0	0.0	6.996370026126564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTAA	45	3.8562575E-10	45.000004	24
ATCGGAT	35	1.2126657E-7	45.000004	34
CTTAACG	40	6.8212103E-9	45.0	1
CGAACTA	20	7.0348097E-4	45.0	23
CGAAACG	20	7.0348097E-4	45.0	45
CGGCATA	20	7.0348097E-4	45.0	27
CCAATCG	55	1.8189894E-12	45.0	24
ATTCGCA	20	7.0348097E-4	45.0	43
CGCGACT	25	3.892069E-5	45.0	22
CCGTACC	25	3.892069E-5	45.0	18
AACGCGT	25	3.892069E-5	45.0	22
TATTACG	40	6.8212103E-9	45.0	1
GAACGTA	50	2.1827873E-11	45.0	36
ACCGTAC	25	3.892069E-5	45.0	19
GCTAACG	75	0.0	45.0	1
TGTCGAC	25	3.892069E-5	45.0	43
CCCGTAG	30	2.1664855E-6	44.999996	18
GCGATAA	245	0.0	41.32653	9
CGCGATT	60	3.6379788E-12	41.249996	43
GTGTACG	60	3.6379788E-12	41.249996	1
>>END_MODULE