##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2363108
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.495637101647493	31.0	31.0	33.0	30.0	34.0
2	31.889963133297336	31.0	31.0	34.0	30.0	34.0
3	31.979924743177207	33.0	31.0	34.0	30.0	34.0
4	35.69465424347935	37.0	35.0	37.0	33.0	37.0
5	35.59053331460094	37.0	35.0	37.0	33.0	37.0
6	35.651597387846856	37.0	35.0	37.0	33.0	37.0
7	36.01047772679031	37.0	35.0	37.0	35.0	37.0
8	36.02267014457232	37.0	35.0	37.0	35.0	37.0
9	37.795013177561074	39.0	38.0	39.0	35.0	39.0
10	37.282947288063006	39.0	37.0	39.0	34.0	39.0
11	36.93012422623088	39.0	37.0	39.0	33.0	39.0
12	36.01187503914336	37.0	35.0	39.0	32.0	39.0
13	35.58928368910773	37.0	35.0	39.0	31.0	39.0
14	36.550642205096004	38.0	35.0	41.0	31.0	41.0
15	36.83613613935546	38.0	35.0	41.0	32.0	41.0
16	37.03184450308661	38.0	35.0	41.0	32.0	41.0
17	36.980251431589245	38.0	35.0	41.0	32.0	41.0
18	36.92211951379285	38.0	35.0	41.0	32.0	41.0
19	36.854089191014545	38.0	35.0	41.0	32.0	41.0
20	36.73052818576214	38.0	35.0	41.0	32.0	41.0
21	36.55951399597479	38.0	35.0	41.0	31.0	41.0
22	36.432764393332846	38.0	35.0	40.0	31.0	41.0
23	36.394270173009446	38.0	35.0	40.0	31.0	41.0
24	36.38781723052861	38.0	35.0	40.0	31.0	41.0
25	36.33468127567593	38.0	35.0	40.0	31.0	41.0
26	36.17590901473822	38.0	35.0	40.0	31.0	41.0
27	36.118414393248216	37.0	35.0	40.0	31.0	41.0
28	36.07475155600167	37.0	35.0	40.0	30.0	41.0
29	36.072703829025166	37.0	35.0	40.0	30.0	41.0
30	36.04019875519866	37.0	35.0	40.0	30.0	41.0
31	35.920560126748335	37.0	35.0	40.0	30.0	41.0
32	35.76421348495287	37.0	34.0	40.0	30.0	41.0
33	35.67034430927406	37.0	34.0	40.0	30.0	41.0
34	35.57297254293921	37.0	34.0	40.0	29.0	41.0
35	35.47312437687994	37.0	34.0	40.0	29.0	41.0
36	35.25638184966578	37.0	34.0	40.0	27.0	41.0
37	35.12653717053981	37.0	34.0	40.0	27.0	41.0
38	35.04947594439188	36.0	34.0	40.0	27.0	41.0
39	35.02670254596912	36.0	34.0	40.0	27.0	41.0
40	34.844769261497994	36.0	34.0	40.0	26.0	41.0
41	34.81866423371255	36.0	34.0	40.0	26.0	41.0
42	34.77139047390132	36.0	34.0	40.0	26.0	41.0
43	34.6379217538936	36.0	34.0	40.0	26.0	41.0
44	34.45571002256351	35.0	34.0	40.0	25.0	41.0
45	34.40186779444697	35.0	34.0	40.0	24.0	41.0
46	34.31939462775294	35.0	34.0	40.0	24.0	41.0
47	34.238402138201046	35.0	33.0	40.0	24.0	41.0
48	34.12269096461101	35.0	33.0	40.0	24.0	41.0
49	34.04155755894356	35.0	33.0	40.0	24.0	41.0
50	33.845259717287576	35.0	33.0	39.0	24.0	41.0
51	33.45098700524902	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	6.0
12	11.0
13	37.0
14	52.0
15	115.0
16	237.0
17	515.0
18	1158.0
19	2224.0
20	3875.0
21	6099.0
22	9137.0
23	13324.0
24	18755.0
25	25989.0
26	33240.0
27	39802.0
28	45438.0
29	51809.0
30	61446.0
31	74878.0
32	94108.0
33	122898.0
34	224958.0
35	365278.0
36	151424.0
37	191056.0
38	293515.0
39	531608.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.16017888306417	22.413956535207024	29.879506141911417	12.546358439817393
2	30.05884623131909	26.2713341920894	29.905742776039013	13.764076800552495
3	29.729957327384106	25.48266943364417	30.487942150760777	14.299431088210948
4	26.99563456261838	27.750445599608653	29.77210521059554	15.481814627177428
5	24.255260445142586	33.24748593801045	27.572332707603714	14.92492090924325
6	22.652752222919986	41.4825306333862	25.19571682716152	10.669000316532296
7	87.82332419847083	4.7149347384884654	5.428571186759132	2.033169876281575
8	88.27721796887828	3.8923316242846284	5.356716662970968	2.473733743866129
9	83.32031375629045	6.22400668949536	7.0631558100603105	3.392523744153886
10	50.0194658898366	27.992330439404377	11.631292348889682	10.356911321869335
11	44.87843974968558	20.371180665462603	20.277702077095082	14.472677507756734
12	40.982934339014555	21.121294498600996	23.05078735292674	14.844983809457716
13	23.91291468692925	37.744191124569845	23.108127093641087	15.23476709485982
14	16.21855624034111	41.18669142502163	26.16888436753631	16.425867967100956
15	14.797842502331676	25.491640669829735	44.703035155397046	15.007481672441546
16	17.32349939147936	21.240036426604288	43.06159515350124	18.374869028415123
17	17.856991724457792	20.987360713094787	27.5650541574909	33.59059340495653
18	22.749362280522092	24.437816638088485	31.694827320630285	21.117993760759134
19	28.162064535349206	26.023905805405423	24.70102932240084	21.11300033684453
20	31.432841833720676	24.403666696570784	24.687741736729766	19.475749732978773
21	23.95870184519709	27.83034038224237	27.156693642440377	21.054264130120163
22	23.322209564691924	23.952692809638833	25.915235359534982	26.80986226613426
23	20.43601054204886	29.76177982555177	24.94794990326299	24.854259729136373
24	21.414679312160086	24.57297762099743	35.265929445459115	18.746413621383365
25	18.890334254718784	25.863566117164343	32.83366651037532	22.41243311774155
26	18.394504186859002	34.32039500522194	25.879265780489085	21.40583502742998
27	19.634650638058016	32.88106172041227	28.095161118323837	19.38912652320588
28	16.047087141171712	28.80228072521442	36.787400321948894	18.363231811664978
29	17.28088601959792	24.744362085863195	35.29250461680126	22.682247277737623
30	20.306647008939073	28.906888724510267	30.3484225012145	20.43804176533616
31	28.600978034012837	26.852179417952964	23.962298803101678	20.58454374493252
32	28.431921012497103	27.680876202018695	25.39659634684492	18.490606438639283
33	25.78223255136879	29.089783454670716	24.598198643481382	20.529785350479116
34	20.09087185181549	29.397302196937254	27.343777770630883	23.168048180616374
35	21.079442835452294	26.89779730761353	29.419857238856622	22.60290261807755
36	28.880821358989937	25.76945277151954	25.672165639488338	19.677560230002182
37	21.62524945960997	31.054314910702345	28.313559938860177	19.006875690827503
38	20.681238436838264	31.029136205370218	24.551692093632624	23.737933264158894
39	21.18172339139811	29.623318104800966	27.68172254505507	21.513235958745856
40	23.91308395553652	26.35080580320493	25.951882013009985	23.784228228248562
41	17.734610521398093	25.345350275992462	28.20725925349159	28.712779949117856
42	22.716989659380783	26.388637336930852	25.220726263886373	25.673646739801992
43	23.721852746467786	26.78565685529396	25.644617173654353	23.847873224583896
44	21.066536104147588	31.019572529059186	26.214206037134147	21.699685329659076
45	18.805615316777736	34.55720178679942	23.147185824769753	23.489997071653093
46	23.07046482852244	31.60448020149735	24.920189851669917	20.40486511831029
47	22.283450438998134	27.51228466917297	27.533993368055963	22.670271523772932
48	23.4963446444259	23.848846519075725	29.800500019465893	22.854308817032486
49	21.738320889269556	23.908471385988285	30.495855458150878	23.857352266591285
50	19.80599278577196	30.511512804323797	27.656543839722943	22.025950570181305
51	18.92397639041466	31.982795538756587	24.72159545818473	24.371632612644024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1498.0
1	2456.0
2	3414.0
3	7755.5
4	12097.0
5	9482.5
6	6868.0
7	6726.5
8	6585.0
9	7103.5
10	7622.0
11	7614.5
12	7607.0
13	7491.5
14	7376.0
15	6924.5
16	6473.0
17	6116.0
18	5759.0
19	6355.0
20	6951.0
21	6659.5
22	6368.0
23	6276.0
24	6184.0
25	8637.0
26	14672.5
27	18255.0
28	23683.5
29	29112.0
30	33051.0
31	36990.0
32	40499.0
33	44008.0
34	51115.0
35	58222.0
36	60037.0
37	61852.0
38	77562.0
39	93272.0
40	123192.0
41	153112.0
42	186862.5
43	220613.0
44	228364.5
45	236116.0
46	233453.5
47	230791.0
48	218605.5
49	206420.0
50	195481.5
51	184543.0
52	167992.5
53	151442.0
54	135922.5
55	120403.0
56	112412.5
57	104422.0
58	96000.5
59	87579.0
60	82729.0
61	77879.0
62	66962.5
63	56046.0
64	45216.0
65	34386.0
66	28842.5
67	23299.0
68	19421.0
69	15543.0
70	12162.0
71	8781.0
72	7361.0
73	5941.0
74	4888.0
75	3024.0
76	2213.0
77	1606.0
78	999.0
79	785.0
80	571.0
81	397.5
82	224.0
83	175.5
84	127.0
85	108.5
86	90.0
87	64.5
88	39.0
89	37.0
90	35.0
91	33.0
92	31.0
93	17.5
94	4.0
95	5.5
96	7.0
97	9.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2363108.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.364002559067416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.40610647513817	15.763480541082902
2	8.36782551568217	3.2406918939899834
3	2.606433017515058	1.5141292686359813
4	1.170500257864286	0.9066227995469242
5	0.6761147305323145	0.6546143686125457
6	0.4253190137993034	0.49415270829778435
7	0.2973132975563476	0.4030022818308121
8	0.2237942226312599	0.34668415197889757
9	0.16395945202281167	0.28574201236977154
>10	1.893878341569143	9.05265932820107
>50	1.1975949516691986	17.282994177320216
>100	1.5621713869645375	48.081405187343044
>500	0.006358311575774229	0.8217780798643222
>1k	0.002192521233025596	0.6612944498846189
>5k	4.3850424660511923E-4	0.4907487510411087
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6389	0.27036428296971615	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5170	0.21877967490271286	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9622006273094585E-4	0.0	0.0	0.058778523876183396	0.0
2	2.9622006273094585E-4	0.0	0.0	0.22665066514099227	0.0
3	2.9622006273094585E-4	0.0	0.0	0.32444560299402314	0.0
4	2.9622006273094585E-4	0.0	0.0	0.5051821584117188	0.0
5	2.9622006273094585E-4	0.0	0.0	0.9010167965238999	0.0
6	2.9622006273094585E-4	0.0	0.0	1.2371842505717048	0.0
7	2.9622006273094585E-4	0.0	0.0	1.4504626957379858	0.0
8	2.9622006273094585E-4	0.0	0.0	2.030334627109722	0.0
9	2.9622006273094585E-4	0.0	0.0	2.2230046193402924	0.0
10	2.9622006273094585E-4	0.0	0.0	2.6479534579037436	0.0
11	2.9622006273094585E-4	0.0	0.0	3.03773674330585	0.0
12	2.9622006273094585E-4	0.0	0.0	3.412158902597765	0.0
13	2.9622006273094585E-4	0.0	0.0	3.560353568266876	0.0
14	2.9622006273094585E-4	0.0	0.0	3.6159159886048373	0.0
15	2.9622006273094585E-4	0.0	0.0	3.716630809933359	0.0
16	2.9622006273094585E-4	0.0	0.0	3.932659869967856	0.0
17	2.9622006273094585E-4	0.0	0.0	4.179326547919096	0.0
18	2.9622006273094585E-4	0.0	0.0	4.551421263860983	0.0
19	2.9622006273094585E-4	0.0	0.0	4.704482401989245	0.0
20	2.9622006273094585E-4	0.0	0.0	4.869265391171288	0.0
21	2.9622006273094585E-4	0.0	0.0	5.071245156801974	0.0
22	2.9622006273094585E-4	0.0	0.0	5.290321051767418	0.0
23	2.9622006273094585E-4	0.0	0.0	5.52518124436124	0.0
24	2.9622006273094585E-4	0.0	0.0	5.695169243216983	0.0
25	2.9622006273094585E-4	0.0	0.0	5.833207792449604	0.0
26	2.9622006273094585E-4	0.0	0.0	5.981952581092358	0.0
27	2.9622006273094585E-4	0.0	0.0	6.117367466912219	0.0
28	2.9622006273094585E-4	0.0	0.0	6.269032139030463	0.0
29	2.9622006273094585E-4	0.0	0.0	6.432249393595215	0.0
30	2.9622006273094585E-4	0.0	0.0	6.6387570944705026	0.0
31	2.9622006273094585E-4	0.0	0.0	6.831977209674717	0.0
32	3.3853721454965243E-4	0.0	0.0	6.998918373599514	0.0
33	3.3853721454965243E-4	0.0	0.0	7.16556331746158	0.0
34	3.3853721454965243E-4	0.0	0.0	7.347780973192931	0.0
35	3.3853721454965243E-4	0.0	0.0	7.572358097894806	0.0
36	3.3853721454965243E-4	0.0	0.0	7.768159559359962	0.0
37	3.3853721454965243E-4	0.0	0.0	7.96286077487783	0.0
38	3.3853721454965243E-4	0.0	0.0	8.159974068049365	0.0
39	3.3853721454965243E-4	0.0	0.0	8.35547930945179	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	25	3.8920607E-5	45.0	24
CCGTGTA	25	3.8920607E-5	45.0	2
CTAACGT	20	7.0348004E-4	45.0	10
GTTCGAC	25	3.8920607E-5	45.0	26
CGTCGGT	25	3.8920607E-5	45.0	19
GAACCGT	25	3.8920607E-5	45.0	3
ATACGTC	20	7.0348004E-4	45.0	11
AATACGC	20	7.0348004E-4	45.0	12
ATCGATT	20	7.0348004E-4	45.0	32
TGCGTAT	20	7.0348004E-4	45.0	1
ACGTTAG	100	0.0	42.75	1
TAATGCG	190	0.0	42.63158	1
CGGGTAT	85	0.0	42.35294	6
CACGACC	300	0.0	42.0	27
GGTTACG	135	0.0	41.666668	1
GACACGA	305	0.0	41.311474	25
CGGGATC	485	0.0	41.28866	6
CGCATGG	345	0.0	41.086956	2
GGCGATA	745	0.0	41.073826	8
CGGTCTA	275	0.0	40.909092	31
>>END_MODULE