FastQCFastQC Report
Sat 18 Jun 2016
SRR3549316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2324182
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC73780.31744501936595326No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70670.3040639674517744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG950.045.00000424
TCAGTCG302.1664637E-645.00000441
AACCGGT253.8920407E-545.028
GTCGACG207.0347765E-445.01
CGCATCG900.045.021
GCACGAC207.0347765E-445.027
CGCTATA207.0347765E-445.013
CCGACGG207.0347765E-445.02
CGTGTAA253.8920407E-545.020
GCTACGA406.8193913E-945.010
CGATACT253.8920407E-545.025
CCCGATA253.8920407E-545.011
ACGTAGG5050.043.2178232
TCGATAG950.042.631581
GTAAACG650.041.538461
TAGTCCG603.6379788E-1241.2500041
CGTAAGG3350.040.970152
GCGTATG1650.040.909091
GGCGATT9850.040.888328
ACGGGAT10150.040.3448265
Produced by FastQC (version 0.11.5)