##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2324182
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529262768578363	31.0	31.0	33.0	30.0	34.0
2	31.93203157067734	31.0	31.0	34.0	30.0	34.0
3	32.02425756674822	33.0	31.0	34.0	30.0	34.0
4	35.72490493429516	37.0	35.0	37.0	35.0	37.0
5	35.62135968697804	37.0	35.0	37.0	33.0	37.0
6	35.68556507192638	37.0	35.0	37.0	33.0	37.0
7	36.05939595091951	37.0	35.0	37.0	35.0	37.0
8	36.07821203330892	37.0	35.0	37.0	35.0	37.0
9	37.88566256859403	39.0	38.0	39.0	35.0	39.0
10	37.33859568656844	39.0	37.0	39.0	34.0	39.0
11	36.94438043148084	39.0	37.0	39.0	33.0	39.0
12	36.018886214590765	37.0	35.0	39.0	32.0	39.0
13	35.62697843800529	37.0	35.0	39.0	31.0	39.0
14	36.59890877736769	38.0	35.0	41.0	31.0	41.0
15	36.90125127894459	38.0	35.0	41.0	32.0	41.0
16	37.07943353833736	38.0	35.0	41.0	32.0	41.0
17	37.03151345290515	38.0	35.0	41.0	32.0	41.0
18	36.968516234959225	38.0	35.0	41.0	32.0	41.0
19	36.91745353849225	38.0	35.0	41.0	32.0	41.0
20	36.79760793259736	38.0	35.0	41.0	32.0	41.0
21	36.64420084141431	38.0	35.0	41.0	32.0	41.0
22	36.525650745079346	38.0	35.0	41.0	31.0	41.0
23	36.473427640348305	38.0	35.0	40.0	31.0	41.0
24	36.49559156726969	38.0	35.0	40.0	31.0	41.0
25	36.45724775426365	38.0	35.0	40.0	31.0	41.0
26	36.32057042004456	38.0	35.0	40.0	31.0	41.0
27	36.25195531158919	38.0	35.0	40.0	31.0	41.0
28	36.21643872984129	38.0	35.0	40.0	31.0	41.0
29	36.211134067813965	38.0	35.0	40.0	31.0	41.0
30	36.16356593416523	37.0	35.0	40.0	31.0	41.0
31	36.061531756118924	37.0	35.0	40.0	30.0	41.0
32	35.92632805864601	37.0	35.0	40.0	30.0	41.0
33	35.84514465734611	37.0	35.0	40.0	30.0	41.0
34	35.749914593607556	37.0	35.0	40.0	30.0	41.0
35	35.65840067602279	37.0	35.0	40.0	30.0	41.0
36	35.47691230721174	37.0	34.0	40.0	29.0	41.0
37	35.355730747419955	37.0	34.0	40.0	28.0	41.0
38	35.293226606178	37.0	34.0	40.0	28.0	41.0
39	35.2821599169084	37.0	34.0	40.0	28.0	41.0
40	35.120144635833164	37.0	34.0	40.0	27.0	41.0
41	35.11746111104896	36.0	34.0	40.0	27.0	41.0
42	35.07083782595339	36.0	34.0	40.0	27.0	41.0
43	34.93487601229164	36.0	34.0	40.0	27.0	41.0
44	34.76781852712051	36.0	34.0	40.0	26.0	41.0
45	34.71830261141339	36.0	34.0	40.0	26.0	41.0
46	34.63819872970361	36.0	34.0	40.0	26.0	41.0
47	34.58246040972695	35.0	34.0	40.0	26.0	41.0
48	34.507895681147176	35.0	34.0	40.0	26.0	41.0
49	34.42786021060313	36.0	34.0	40.0	25.0	41.0
50	34.24608658013873	35.0	33.0	40.0	24.0	41.0
51	33.85784590019198	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	7.0
12	8.0
13	9.0
14	29.0
15	66.0
16	159.0
17	387.0
18	938.0
19	1920.0
20	3252.0
21	5369.0
22	8005.0
23	11758.0
24	16444.0
25	23185.0
26	30565.0
27	36851.0
28	42159.0
29	48309.0
30	58133.0
31	71382.0
32	88622.0
33	117224.0
34	218459.0
35	360789.0
36	144836.0
37	185986.0
38	291386.0
39	557787.0
40	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.94810819462503	23.984266292398786	30.52626687582986	12.541358637146317
2	29.088556748137623	26.54228455430771	31.081860198555876	13.287298498998787
3	29.131410534975316	25.46078577323118	31.472449231600624	13.935354460192876
4	26.49616079979967	27.44733415885675	31.251511284400273	14.804993756943302
5	23.47419436171522	33.436236921204966	28.202782742487464	14.886785974592351
6	21.724073243833743	42.55768265996381	24.871374100651327	10.846869995551122
7	89.47384499148518	4.138961578740391	4.874618252787432	1.5125751769870002
8	90.53038875613011	3.055225451363103	4.566681955199722	1.8477038373070611
9	85.89761903327708	5.434600216334177	6.039888442471373	2.6278923079173664
10	50.35431820743814	30.422531454077177	10.26262142981918	8.9605289086655
11	44.51329543039228	19.058920514830593	22.286851890256443	14.140932164520677
12	40.290261261811686	20.01267542731163	24.378942785031466	15.318120525845222
13	23.827049688879786	36.67255834525868	24.3646151635285	15.135776802333037
14	16.690603403692137	40.32244462783035	27.28478234492824	15.702169623549276
15	14.767647284076721	25.15000976687712	45.24331571279701	14.83902723624914
16	17.91937980760543	20.893888688579466	43.39797829946192	17.78875320435319
17	18.092128757558573	20.882615905294852	27.932795280231925	33.092460056914646
18	22.55223558223926	24.089163413192257	32.54714992199406	20.81145108257443
19	27.37410409339716	26.04993929046865	26.069516070600322	20.50644054553387
20	30.376450725459534	24.053451924160846	25.694244254537722	19.87585309584189
21	23.256483356294815	27.36472444929012	28.376004977235002	21.002787217180067
22	23.220384634249815	24.485302786098508	27.142108492364198	25.152204087287483
23	19.753874696559908	28.812115402322192	26.80185975108662	24.63215015003128
24	21.161423675082244	24.683006752483237	35.43164864025278	18.72392093218173
25	17.808200906813664	26.091115067580766	33.14839371443372	22.952290311171843
26	17.737122135874042	33.7852199182336	27.01767761732945	21.459980328562906
27	18.55573272661091	32.70578637989624	28.66096544934949	20.07751544414336
28	15.968542911011271	28.607054008679185	36.918279205329014	18.50612387498053
29	17.25501703395001	24.60000980990301	36.10715511952162	22.03781803662536
30	19.11046553152894	28.115784392100103	32.186549934557625	20.587200141813337
31	28.365893892991167	26.345957416415754	24.404112930915048	20.884035759678028
32	27.73177832028645	27.9584817368003	26.01822920924437	18.29151073366888
33	25.103154572232295	28.57444038375652	26.541510088280525	19.78089495573066
34	19.778528531758703	28.876740289701925	28.499532308571357	22.845198869968016
35	19.828610668183472	26.88872902380278	30.809764467670774	22.472895840342968
36	27.625074112096215	25.568221421558206	27.98464147816307	18.82206298818251
37	21.246614938072835	31.057163337466687	28.944549092971204	18.75167263148927
38	20.44771020513884	31.95567300667504	24.83957796764625	22.757038820539872
39	21.224241475065206	29.764622563981654	27.60218433840379	21.408951622549353
40	22.704977493156733	27.177906033176402	25.928950486665848	24.188165987001017
41	17.572246923863965	25.63603022482749	28.371487258743077	28.42023559256547
42	22.3801320206421	26.569347839368863	25.549591211015315	25.50092892897372
43	22.480726552395637	26.55626796868748	26.972242277067803	23.99076320184908
44	20.364153925983423	30.675652767296192	27.379095096683475	21.58109821003691
45	18.94313784376611	34.46933157558229	23.903936955023315	22.683593625628284
46	22.506800241977608	31.207280669069803	26.370654277504947	19.915264811447642
47	21.81270657805628	27.56595653868759	27.91506861338742	22.70626826986871
48	23.083519276889675	24.64411134756228	29.55994840335223	22.712420972195808
49	20.69158095192201	24.59114647648076	30.65887267004047	24.05839990155676
50	19.3369538185908	30.61059762101247	28.052579359103547	21.999869201293187
51	18.59815625454461	32.0606561792493	25.84831996805758	23.49286759814851
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	728.0
1	1933.0
2	3138.0
3	7965.5
4	12793.0
5	9655.0
6	6517.0
7	6408.5
8	6300.0
9	6497.0
10	6694.0
11	6677.0
12	6660.0
13	6496.0
14	6332.0
15	6122.5
16	5913.0
17	5615.0
18	5317.0
19	5312.5
20	5308.0
21	7327.5
22	9347.0
23	8920.5
24	8494.0
25	11222.5
26	17386.5
27	20822.0
28	25105.0
29	29388.0
30	34295.0
31	39202.0
32	44080.5
33	48959.0
34	54403.5
35	59848.0
36	65619.0
37	71390.0
38	87352.5
39	103315.0
40	132489.0
41	161663.0
42	195681.0
43	229699.0
44	237714.0
45	245729.0
46	239359.0
47	232989.0
48	216508.5
49	200028.0
50	184228.5
51	168429.0
52	152518.0
53	136607.0
54	123295.0
55	109983.0
56	101914.0
57	93845.0
58	86451.0
59	79057.0
60	71788.5
61	64520.0
62	56389.0
63	48258.0
64	40869.5
65	33481.0
66	26081.0
67	18681.0
68	15135.5
69	11590.0
70	9706.5
71	7823.0
72	6059.0
73	4295.0
74	3766.5
75	2547.5
76	1857.0
77	1366.5
78	876.0
79	614.0
80	352.0
81	394.5
82	437.0
83	305.0
84	173.0
85	127.0
86	81.0
87	59.5
88	38.0
89	31.0
90	24.0
91	15.5
92	7.0
93	5.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2324182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.188123087060845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15386658137827	15.571903809543999
2	8.337433811484425	3.19959412409972
3	2.6347226438405804	1.516661471708383
4	1.1833578183020361	0.9082566189446103
5	0.6909376478864587	0.6628898316564837
6	0.42356640105515964	0.4876466543393869
7	0.3116104755493802	0.418545411204135
8	0.22518139691595795	0.3456646688751764
9	0.17458712291773332	0.3014999283565159
>10	2.0230012581942316	9.611374645724586
>50	1.2166189483793741	17.389240248352856
>100	1.6181382497000223	48.04282234697756
>500	0.005627132577698379	0.6668481189734621
>1k	9.003412124317406E-4	0.2531776990558572
>5k	4.501706062158703E-4	0.6238744221872837
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7378	0.31744501936595326	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7067	0.3040639674517744	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7210356159715547E-4	0.0	0.0	0.040874595879324425	0.0
2	1.7210356159715547E-4	0.0	0.0	0.15059061639751103	0.0
3	1.7210356159715547E-4	0.0	0.0	0.21943204103637323	0.0
4	1.7210356159715547E-4	0.0	0.0	0.36275128195640444	0.0
5	1.7210356159715547E-4	0.0	0.0	0.6477978058516932	0.0
6	1.7210356159715547E-4	0.0	0.0	0.935684038513335	0.0
7	1.7210356159715547E-4	0.0	0.0	1.1197487976414928	0.0
8	1.7210356159715547E-4	0.0	0.0	1.654646667085452	0.0
9	1.7210356159715547E-4	0.0	0.0	1.849166717580637	0.0
10	1.7210356159715547E-4	0.0	0.0	2.2262886469304037	0.0
11	1.7210356159715547E-4	0.0	0.0	2.6090039420320785	0.0
12	1.7210356159715547E-4	0.0	0.0	2.940045142764207	0.0
13	1.7210356159715547E-4	0.0	0.0	3.0730381699884086	0.0
14	1.7210356159715547E-4	0.0	0.0	3.1173548370996764	0.0
15	1.7210356159715547E-4	0.0	0.0	3.2013844010494874	0.0
16	1.7210356159715547E-4	0.0	0.0	3.395775373873475	0.0
17	1.7210356159715547E-4	0.0	0.0	3.6233823340857128	0.0
18	1.7210356159715547E-4	0.0	0.0	3.947797547696351	0.0
19	1.7210356159715547E-4	0.0	0.0	4.097570672176276	0.0
20	1.7210356159715547E-4	0.0	0.0	4.264941385829509	0.0
21	1.7210356159715547E-4	0.0	0.0	4.4722831516636825	0.0
22	1.7210356159715547E-4	0.0	0.0	4.692446632836843	0.0
23	1.7210356159715547E-4	0.0	0.0	4.938597751811175	0.0
24	1.7210356159715547E-4	0.0	0.0	5.109668692038747	0.0
25	1.7210356159715547E-4	0.0	0.0	5.255999745286728	0.0
26	1.7210356159715547E-4	0.0	0.0	5.402029617301915	0.0
27	1.7210356159715547E-4	0.0	0.0	5.538722871100456	0.0
28	1.7210356159715547E-4	0.0	0.0	5.690862419552341	0.0
29	1.7210356159715547E-4	0.0	0.0	5.859997194711946	0.0
30	1.7210356159715547E-4	0.0	0.0	6.059293119041452	0.0
31	1.7210356159715547E-4	0.0	0.0	6.253942247207835	0.0
32	1.7210356159715547E-4	0.0	0.0	6.422947944696242	0.0
33	1.7210356159715547E-4	0.0	0.0	6.600214613141311	0.0
34	1.7210356159715547E-4	0.0	0.0	6.792454291445335	0.0
35	1.7210356159715547E-4	0.0	0.0	7.021954390835141	0.0
36	1.7210356159715547E-4	0.0	0.0	7.223358583794212	0.0
37	1.7210356159715547E-4	0.0	0.0	7.426828019492449	0.0
38	2.1512945199644433E-4	0.0	0.0	7.627242616972337	0.0
39	2.1512945199644433E-4	0.0	0.0	7.823913961987486	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATCG	95	0.0	45.000004	24
TCAGTCG	30	2.1664637E-6	45.000004	41
AACCGGT	25	3.8920407E-5	45.0	28
GTCGACG	20	7.0347765E-4	45.0	1
CGCATCG	90	0.0	45.0	21
GCACGAC	20	7.0347765E-4	45.0	27
CGCTATA	20	7.0347765E-4	45.0	13
CCGACGG	20	7.0347765E-4	45.0	2
CGTGTAA	25	3.8920407E-5	45.0	20
GCTACGA	40	6.8193913E-9	45.0	10
CGATACT	25	3.8920407E-5	45.0	25
CCCGATA	25	3.8920407E-5	45.0	11
ACGTAGG	505	0.0	43.217823	2
TCGATAG	95	0.0	42.63158	1
GTAAACG	65	0.0	41.53846	1
TAGTCCG	60	3.6379788E-12	41.250004	1
CGTAAGG	335	0.0	40.97015	2
GCGTATG	165	0.0	40.90909	1
GGCGATT	985	0.0	40.88832	8
ACGGGAT	1015	0.0	40.344826	5
>>END_MODULE