##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2427688
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.571024777483764	31.0	31.0	33.0	30.0	34.0
2	31.987715884413483	31.0	31.0	34.0	30.0	34.0
3	32.095468198549405	33.0	31.0	34.0	30.0	34.0
4	35.820805227030824	37.0	35.0	37.0	35.0	37.0
5	35.743267256747984	37.0	35.0	37.0	35.0	37.0
6	35.81219868450971	37.0	35.0	37.0	35.0	37.0
7	36.0803995406329	37.0	35.0	37.0	35.0	37.0
8	36.08515632980844	37.0	35.0	37.0	35.0	37.0
9	37.87220351214818	39.0	38.0	39.0	35.0	39.0
10	37.41208178316159	39.0	37.0	39.0	35.0	39.0
11	37.095309199534704	39.0	37.0	39.0	33.0	39.0
12	36.48527570264383	38.0	35.0	39.0	33.0	39.0
13	36.24061741047449	39.0	35.0	39.0	32.0	39.0
14	37.3303447559983	39.0	35.0	41.0	32.0	41.0
15	37.502536981687925	39.0	35.0	41.0	33.0	41.0
16	37.61160701045604	39.0	35.0	41.0	33.0	41.0
17	37.54497241820201	39.0	35.0	41.0	33.0	41.0
18	37.47390644926366	39.0	35.0	41.0	33.0	41.0
19	37.42861768069044	39.0	35.0	41.0	33.0	41.0
20	37.32862336511117	39.0	35.0	41.0	33.0	41.0
21	37.20249636691371	39.0	35.0	41.0	32.0	41.0
22	37.09556623421132	39.0	35.0	41.0	32.0	41.0
23	37.006543262560925	39.0	35.0	41.0	32.0	41.0
24	37.017796767953705	39.0	35.0	41.0	32.0	41.0
25	36.988869657056426	39.0	35.0	41.0	32.0	41.0
26	36.85350671091178	38.0	35.0	41.0	32.0	41.0
27	36.79175577751342	38.0	35.0	41.0	31.0	41.0
28	36.72482666635911	38.0	35.0	41.0	31.0	41.0
29	36.67654533861023	38.0	35.0	41.0	31.0	41.0
30	36.618887600054045	38.0	35.0	40.0	31.0	41.0
31	36.54672099544917	38.0	35.0	41.0	31.0	41.0
32	36.410844391865844	38.0	35.0	41.0	31.0	41.0
33	36.332947644013565	38.0	35.0	41.0	30.0	41.0
34	36.22086734374434	38.0	35.0	41.0	30.0	41.0
35	36.1000412738375	38.0	35.0	41.0	30.0	41.0
36	35.94831337470054	38.0	35.0	40.0	30.0	41.0
37	35.82434851595428	38.0	35.0	40.0	29.0	41.0
38	35.748305383558346	38.0	35.0	40.0	29.0	41.0
39	35.71577978718847	38.0	35.0	40.0	29.0	41.0
40	35.58223214844741	38.0	35.0	40.0	28.0	41.0
41	35.54171046691338	38.0	34.0	40.0	28.0	41.0
42	35.48969636954996	38.0	34.0	40.0	28.0	41.0
43	35.395070124332285	38.0	34.0	40.0	28.0	41.0
44	35.217701780459436	37.0	34.0	40.0	27.0	41.0
45	35.177183394241766	37.0	34.0	40.0	27.0	41.0
46	35.11513876577221	37.0	34.0	40.0	27.0	41.0
47	35.02735194967393	37.0	34.0	40.0	27.0	41.0
48	34.894065052840396	37.0	34.0	40.0	26.0	41.0
49	34.803326045191966	37.0	34.0	40.0	26.0	41.0
50	34.6559232487865	36.0	34.0	40.0	26.0	41.0
51	34.29089899525804	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	5.0
12	5.0
13	17.0
14	26.0
15	63.0
16	138.0
17	370.0
18	821.0
19	1616.0
20	2773.0
21	4571.0
22	6867.0
23	10037.0
24	14508.0
25	20819.0
26	27261.0
27	33168.0
28	37912.0
29	44234.0
30	53607.0
31	66319.0
32	84246.0
33	112814.0
34	203272.0
35	299182.0
36	169470.0
37	225237.0
38	357225.0
39	650948.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.713805068855635	24.04209272361193	30.160259473210726	12.08384273432171
2	30.406419605814257	25.689256609580802	30.45869156168338	13.44563222292156
3	30.35674271158403	25.25262719097347	30.57954728943752	13.811082808004983
4	27.95173844414933	27.433261605280414	29.683550769291607	14.93144918127865
5	23.76260870424865	33.475512504077955	27.749900316679906	15.011978474993493
6	22.359504186699443	42.36611129601498	24.795031321982066	10.479353195303515
7	88.99945132982492	4.182539107166983	5.063212406207058	1.7547971568010388
8	89.69323899941014	3.5433301149076812	4.986596300677847	1.7768345850043332
9	83.854020780265	6.731631082742099	6.48180491068045	2.932543226312442
10	44.33267372084057	34.266594389394356	11.451018417523175	9.949713472241903
11	37.9075482516699	21.199882357205706	24.171845805556565	16.720723585567832
12	34.27005447157954	21.173643400634678	27.238096493453856	17.318205634331925
13	23.8762971189049	31.483946866318902	26.854274519625253	17.785481495150943
14	18.27903750399557	35.16642171481673	28.91932571236502	17.63521506882268
15	17.413316702970068	25.83301478608454	40.34933648804954	16.404332022895858
16	21.207132053212767	23.313003977446854	37.64627085523345	17.833593114106918
17	20.975883227169223	22.930211789982895	28.22224272641295	27.87166225643493
18	22.854254747727058	25.453682680805773	31.71194156745018	19.98012100401699
19	26.72991751823134	25.04333341022405	27.618540768006433	20.60820830353818
20	28.27257868391655	25.256869910795785	26.863748554179946	19.606802851107723
21	24.973596277610632	25.81727965043284	29.09937356035866	20.109750511597866
22	24.863944625503773	23.202404921884526	28.17602591436791	23.757624538243793
23	20.96842757388923	27.53624024174441	28.044089685330242	23.45124249903612
24	21.032480285769836	25.717102032880668	33.86468936700268	19.38572831434682
25	20.52084122836213	26.689549892737453	31.632771591736663	21.156837287163754
26	19.723498241948718	31.880414616705277	27.08235160366571	21.313735537680294
27	20.094592056310365	30.44102042766616	29.092412204533698	20.37197531148978
28	18.000171356451077	28.045737343513665	34.76608196769931	19.18800933233595
29	19.735896869778983	25.681141893027444	33.43979951295224	21.143161724241335
30	20.80271435209137	27.612815155819035	30.93861319906018	20.64585729302942
31	27.03024441361493	25.53174048724548	26.734160238053654	20.703854861085937
32	26.61552884884713	27.56054319994991	27.35417401247607	18.46975393872689
33	25.051036212231555	27.781700119619984	27.03296304961758	20.134300618530883
34	21.33099475715166	27.979007187085	28.890656459973442	21.799341595789905
35	21.713539795888103	26.68617219346143	29.950553777915452	21.649734232735014
36	27.16691765992994	25.925366027265444	27.566639535228582	19.34107677757603
37	21.96674366722577	30.770428489987182	27.37691169540732	19.885916147379728
38	21.77553293503943	30.916740536675224	24.914527731734886	22.39319879655046
39	21.98387931233338	29.40806232102313	27.460736305489007	21.14732206115448
40	23.0205858413437	27.13211088080511	27.030038456342005	22.81726482150919
41	19.556137361967433	26.438158445401548	28.209761715673515	25.7959424769575
42	22.449713472241903	26.847519121073223	26.43103232375824	24.27173508292664
43	23.185022128049404	25.884545295771122	27.245346189460918	23.685086386718556
44	21.377293952105873	28.786359696962705	28.307220697223034	21.529125653708384
45	20.131746748346576	32.146181881691554	25.953211450565313	21.76885991939656
46	23.05732861883405	29.414076273392624	27.02950296743239	20.49909214034093
47	22.623623793502297	27.827175485482485	27.42362280490738	22.125577916107837
48	22.977417196937992	25.512668843772346	29.535797021693067	21.974116937596595
49	21.35121976135319	25.376984192367384	30.752716164515377	22.51907988176405
50	20.57846807332738	29.301458836555604	28.620605283710265	21.499467806406756
51	20.9533515015109	29.989150170862157	26.51366238165695	22.54383594596999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1457.0
1	2823.0
2	4189.0
3	9956.0
4	15723.0
5	11805.5
6	7888.0
7	7501.5
8	7115.0
9	7178.0
10	7241.0
11	7134.5
12	7028.0
13	6958.0
14	6888.0
15	6446.0
16	6004.0
17	6035.5
18	6067.0
19	6203.5
20	6340.0
21	7063.5
22	7787.0
23	9243.5
24	10700.0
25	14338.5
26	20620.0
27	23263.0
28	30166.0
29	37069.0
30	44502.5
31	51936.0
32	56562.5
33	61189.0
34	66512.5
35	71836.0
36	79417.5
37	86999.0
38	94082.5
39	101166.0
40	130569.0
41	159972.0
42	181690.0
43	203408.0
44	209347.0
45	215286.0
46	211223.0
47	207160.0
48	195856.5
49	184553.0
50	179913.0
51	175273.0
52	162954.5
53	150636.0
54	137559.5
55	124483.0
56	118587.5
57	112692.0
58	101818.5
59	90945.0
60	84495.5
61	78046.0
62	68029.5
63	58013.0
64	50974.5
65	43936.0
66	35806.5
67	27677.0
68	22947.0
69	18217.0
70	15517.0
71	12817.0
72	10560.0
73	8303.0
74	6650.5
75	3761.5
76	2525.0
77	1944.0
78	1363.0
79	1038.5
80	714.0
81	554.5
82	395.0
83	295.5
84	196.0
85	155.0
86	114.0
87	77.5
88	41.0
89	36.0
90	31.0
91	22.0
92	13.0
93	13.0
94	13.0
95	8.5
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2427688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.147250363342444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.60737294816616	14.809494283850306
2	6.600013032598985	2.395441778077936
3	1.8890494145096197	1.0284315802149457
4	0.8960362514457563	0.65042376758468
5	0.53846577817642	0.4885836644329756
6	0.3871340147743708	0.4215250734165852
7	0.294520937630301	0.37413216346964057
8	0.24398040028891163	0.3542058726233109
9	0.21113543062171192	0.34483747680578924
>10	3.8177215837950507	18.48786274653977
>50	2.358604699233743	30.974282829567013
>100	1.1486680348241298	27.96579565862347
>500	0.005701151511580496	0.7420167253804762
>1k	0.001368276362779319	0.6240720702028768
>5k	2.280460604632198E-4	0.3388943092103022
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8189	0.3373168216014578	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3934	0.16204718233974053	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2689	0.11076382138067165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2357436375679248E-4	0.0	0.0	0.01870092038186126	0.0
2	1.2357436375679248E-4	0.0	0.0	0.06434105206270328	0.0
3	1.2357436375679248E-4	0.0	0.0	0.09387532500057669	0.0
4	1.2357436375679248E-4	0.0	0.0	0.14503511159588878	0.0
5	1.2357436375679248E-4	0.0	0.0	0.2673737317151133	0.0
6	1.2357436375679248E-4	0.0	0.0	0.39655013329554706	0.0
7	1.2357436375679248E-4	0.0	0.0	0.4805807006501659	0.0
8	1.2357436375679248E-4	0.0	0.0	0.7034676614128339	0.0
9	1.2357436375679248E-4	0.0	0.0	0.7947891162291035	0.0
10	1.2357436375679248E-4	0.0	0.0	0.9612437842095031	0.0
11	1.2357436375679248E-4	0.0	0.0	1.130293513828795	0.0
12	1.2357436375679248E-4	0.0	0.0	1.2850086172522992	0.0
13	1.2357436375679248E-4	0.0	0.0	1.351985922408481	0.0
14	1.2357436375679248E-4	0.0	0.0	1.3782248789795064	0.0
15	1.2357436375679248E-4	0.0	0.0	1.4261305406625564	0.0
16	1.2357436375679248E-4	0.0	0.0	1.5264729240330719	0.0
17	1.2357436375679248E-4	0.0	0.0	1.6500472877898642	0.0
18	1.2357436375679248E-4	0.0	0.0	1.817696507953246	0.0
19	1.2357436375679248E-4	0.0	0.0	1.9021389898537209	0.0
20	1.2357436375679248E-4	0.0	0.0	1.9972088670372798	0.0
21	1.2357436375679248E-4	0.0	0.0	2.111515153512313	0.0
22	1.2357436375679248E-4	0.0	0.0	2.2366959839979437	0.0
23	1.2357436375679248E-4	0.0	0.0	2.3694560421273243	0.0
24	1.2357436375679248E-4	0.0	0.0	2.4779543335057883	0.0
25	1.2357436375679248E-4	0.0	0.0	2.5647447283176423	0.0
26	1.2357436375679248E-4	0.0	0.0	2.6568488207710383	0.0
27	1.2357436375679248E-4	0.0	0.0	2.7464814259492982	0.0
28	1.2357436375679248E-4	0.0	0.0	2.847318106774841	0.0
29	1.2357436375679248E-4	0.0	0.0	2.954127548515295	0.0
30	1.2357436375679248E-4	0.0	0.0	3.081120803002692	0.0
31	1.2357436375679248E-4	0.0	0.0	3.204571592395728	0.0
32	1.2357436375679248E-4	0.0	0.0	3.3199076652354007	0.0
33	1.2357436375679248E-4	0.0	0.0	3.444429432447662	0.0
34	1.2357436375679248E-4	0.0	0.0	3.570722432207104	0.0
35	1.2357436375679248E-4	0.0	0.0	3.7158811181667497	0.0
36	1.2357436375679248E-4	0.0	0.0	3.851936492662978	0.0
37	1.2357436375679248E-4	0.0	0.0	3.985685145702413	0.0
38	1.2357436375679248E-4	0.0	0.0	4.126642303294328	0.0
39	1.6476581834238996E-4	0.0	0.0	4.274272476529109	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	30	2.1665037E-6	45.000004	1
TCATACG	45	3.8562575E-10	45.000004	1
TAGTCCG	25	3.8920916E-5	45.000004	1
CTATCGT	20	7.034839E-4	45.0	27
GCGATCG	20	7.034839E-4	45.0	9
CGTAAGG	385	0.0	41.493504	2
CGTTAGG	440	0.0	40.397728	2
TGCGAAG	390	0.0	40.384617	1
TACGGGA	980	0.0	40.17857	4
CACGACG	320	0.0	40.078125	26
TCGGACG	45	1.9297659E-8	40.000004	1
AACGGGA	955	0.0	39.581154	4
CGGTCTA	330	0.0	39.545456	31
AATACGG	190	0.0	39.078945	2
CGTTTTT	4935	0.0	39.02736	1
CGACGGT	325	0.0	38.76923	28
ACGGGAT	1305	0.0	38.620693	5
TTGCGAG	210	0.0	38.57143	1
CGAACGG	140	0.0	38.571426	2
TAAGTCG	70	0.0	38.571426	1
>>END_MODULE