##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1336622
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.535344323226763	31.0	31.0	33.0	30.0	34.0
2	31.949382847207364	31.0	31.0	34.0	30.0	34.0
3	32.04997299161618	33.0	31.0	34.0	30.0	34.0
4	35.76106707805199	37.0	35.0	37.0	35.0	37.0
5	35.67801891634284	37.0	35.0	37.0	33.0	37.0
6	35.731580805942144	37.0	35.0	37.0	33.0	37.0
7	36.037192265277696	37.0	35.0	37.0	35.0	37.0
8	36.03858308482129	37.0	35.0	37.0	35.0	37.0
9	37.81632129352951	39.0	38.0	39.0	35.0	39.0
10	37.3385886211659	39.0	37.0	39.0	34.0	39.0
11	37.02232643185583	39.0	37.0	39.0	33.0	39.0
12	36.27794769201764	38.0	35.0	39.0	32.0	39.0
13	35.98709657629457	38.0	35.0	39.0	32.0	39.0
14	37.02566394986765	39.0	35.0	41.0	32.0	41.0
15	37.235608122565694	39.0	35.0	41.0	32.0	41.0
16	37.382165638452754	39.0	35.0	41.0	33.0	41.0
17	37.31857922434316	39.0	35.0	41.0	33.0	41.0
18	37.26106109281457	39.0	35.0	41.0	32.0	41.0
19	37.20687898298846	39.0	35.0	41.0	32.0	41.0
20	37.08826354795896	39.0	35.0	41.0	32.0	41.0
21	36.95040258203142	38.0	35.0	41.0	32.0	41.0
22	36.83112503011323	38.0	35.0	41.0	32.0	41.0
23	36.77198115847263	38.0	35.0	41.0	31.0	41.0
24	36.772712853746235	38.0	35.0	41.0	32.0	41.0
25	36.741946488984915	38.0	35.0	41.0	31.0	41.0
26	36.59923598444437	38.0	35.0	41.0	31.0	41.0
27	36.53084417284767	38.0	35.0	41.0	31.0	41.0
28	36.462866090787074	38.0	35.0	40.0	31.0	41.0
29	36.41949182341754	38.0	35.0	40.0	31.0	41.0
30	36.37325287179173	38.0	35.0	40.0	31.0	41.0
31	36.283190011835806	38.0	35.0	40.0	30.0	41.0
32	36.1390078870466	38.0	35.0	40.0	30.0	41.0
33	36.07643148175026	38.0	35.0	40.0	30.0	41.0
34	35.97049053509519	38.0	35.0	40.0	30.0	41.0
35	35.87598438451559	38.0	35.0	40.0	30.0	41.0
36	35.69693002210049	38.0	35.0	40.0	29.0	41.0
37	35.603905217780344	38.0	34.0	40.0	29.0	41.0
38	35.52731811985737	38.0	34.0	40.0	29.0	41.0
39	35.49339529051594	38.0	34.0	40.0	28.0	41.0
40	35.33170859076089	37.0	34.0	40.0	27.0	41.0
41	35.301866197025035	37.0	34.0	40.0	27.0	41.0
42	35.24708705976708	37.0	34.0	40.0	27.0	41.0
43	35.13897870901422	37.0	34.0	40.0	27.0	41.0
44	34.97110776270329	37.0	34.0	40.0	27.0	41.0
45	34.92193754105499	37.0	34.0	40.0	26.0	41.0
46	34.835167309830304	36.0	34.0	40.0	26.0	41.0
47	34.76993944436048	36.0	34.0	40.0	26.0	41.0
48	34.670580014394496	36.0	34.0	40.0	26.0	41.0
49	34.574647132846835	36.0	34.0	40.0	26.0	41.0
50	34.418727209338165	36.0	34.0	40.0	25.0	41.0
51	34.052896780091906	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	9.0
13	16.0
14	34.0
15	64.0
16	108.0
17	256.0
18	536.0
19	1084.0
20	1904.0
21	2963.0
22	4522.0
23	6480.0
24	9121.0
25	12724.0
26	16846.0
27	20062.0
28	22589.0
29	26280.0
30	31332.0
31	38795.0
32	48449.0
33	63530.0
34	115911.0
35	181202.0
36	88606.0
37	116576.0
38	183445.0
39	343091.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.881814005754805	23.95045121208539	28.817721090929222	12.35001369123058
2	30.744892722100936	26.12301757714597	29.329309258713383	13.802780442039708
3	30.066017168653513	25.821885319858566	30.36378272989671	13.748314781591207
4	27.15621918537926	27.546232218233722	29.528318402659842	15.769230193727171
5	23.852442949465143	33.52630736288943	27.6970602010142	14.924189486631223
6	23.006429641289756	41.19115202353395	25.204059188012767	10.59835914716352
7	88.25763753701496	4.59673714782489	5.217032190103111	1.9285931250570467
8	88.4848521122651	4.018039505559537	5.3225968149559115	2.174511567219453
9	83.08295090160121	6.673165636956446	6.811649067574827	3.432234393867526
10	46.80343432922696	30.8458187879595	12.08786029258833	10.262886590225209
11	40.52334915929859	21.62496203115017	22.150765137787648	15.700923671763595
12	38.04411419234458	21.41046608540036	24.994800324998394	15.550619397256666
13	24.023994816784402	34.31912687356635	24.310089165074345	17.346789144574906
14	17.179651389846942	36.983305676548795	27.020279480660953	18.816763452943317
15	16.15976693485518	26.156011198379197	41.414925087272245	16.26929677949338
16	19.7018304352315	22.316705845033226	39.471892576958936	18.509571142776345
17	19.45553791573085	22.286330765167715	27.897191576975388	30.360939742126046
18	22.553047907336556	24.758084185356818	31.597115714091196	21.091752193215434
19	27.941631964758923	25.390499333394185	25.68422485938433	20.983643842462566
20	30.19814128452173	24.326773014360082	25.40314314742687	20.071942553691322
21	24.871579249780417	27.398322038691568	26.80346425541402	20.926634456113995
22	23.274418646408634	24.999513699460284	25.981242265951032	25.744825388180054
23	21.555608092639506	28.991966315083843	25.65482238059825	23.797603211678396
24	21.32532608321575	24.822575118470294	33.99884185656079	19.853256941753166
25	19.714997957537733	26.92967794933796	31.13041682689646	22.22490726622785
26	18.94537124183202	32.791918732446426	26.394597724712	21.86811230100956
27	20.213717864886256	31.901614667422802	27.977543389230462	19.907124078460477
28	17.518191380958864	29.139128339949515	34.142712000849905	19.199968278241716
29	18.834868796114385	26.016330720278436	33.427476130125044	21.721324353482135
30	21.12489544538396	28.710360894852844	30.18759230358321	19.97715135617998
31	27.589550374002524	26.990727370939577	24.537528186727435	20.882194068330463
32	28.103757083154402	27.816465687382074	25.47391857982287	18.60585864964066
33	26.537046375115775	28.182388139653547	25.04649781314388	20.2340676720868
34	20.559514956360136	28.587663527908415	28.335685032866436	22.517136482865013
35	21.926842443114058	26.698797416173008	29.093939797489494	22.28042034322344
36	26.819774027361515	28.252190970970105	25.627963627712248	19.300071373956136
37	22.424140856577253	31.474942055420307	26.70463302264963	19.396284065352805
38	21.903724463610505	31.774652818822375	24.566032879901723	21.755589837665397
39	22.137822061884364	29.944067956385574	26.371479745208443	21.54663023652162
40	23.814960400172975	27.682246738419686	26.075135677850582	22.427657183556757
41	19.45471494558671	26.43933737436613	27.564487192340092	26.54146048770707
42	23.001417004957272	26.898105821990058	24.567379558319406	25.533097614733258
43	23.619991291479568	27.317446518162953	24.863050286468425	24.199511903889057
44	21.156617203667157	30.119510228022584	26.707251564017355	22.01662100429291
45	19.9094433579576	33.35363326355544	24.23841594706656	22.4985074314204
46	22.112684064754283	31.770463152634026	25.193510207074247	20.923342575537436
47	22.775249846254216	27.17888827207692	27.158314018473433	22.88754786319543
48	23.239180561145933	24.795192657310743	29.68468273004634	22.28094405149698
49	21.44727529548369	25.696270149675826	29.809549745552594	23.04690480928789
50	19.629708324417823	30.761426940451376	28.099342970563107	21.509521764567694
51	19.98141583783598	31.07168668479196	25.510054450697357	23.436843026674705
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	516.0
1	1274.5
2	2033.0
3	4547.5
4	7062.0
5	5546.0
6	4030.0
7	3799.5
8	3569.0
9	3677.5
10	3786.0
11	3828.0
12	3870.0
13	3697.0
14	3524.0
15	3509.5
16	3495.0
17	3385.0
18	3275.0
19	3401.0
20	3527.0
21	3460.5
22	3394.0
23	4352.0
24	5310.0
25	6787.5
26	10161.0
27	12057.0
28	14294.0
29	16531.0
30	21250.0
31	25969.0
32	28634.0
33	31299.0
34	37201.0
35	43103.0
36	44718.5
37	46334.0
38	51030.0
39	55726.0
40	70519.5
41	85313.0
42	99989.5
43	114666.0
44	117111.5
45	119557.0
46	114932.0
47	110307.0
48	104797.0
49	99287.0
50	97639.5
51	95992.0
52	91654.0
53	87316.0
54	78746.5
55	70177.0
56	67030.0
57	63883.0
58	59253.0
59	54623.0
60	49858.5
61	45094.0
62	40687.0
63	36280.0
64	29919.5
65	23559.0
66	19278.0
67	14997.0
68	12938.0
69	10879.0
70	8865.5
71	6852.0
72	5592.0
73	4332.0
74	3701.5
75	2338.5
76	1606.0
77	1418.0
78	1230.0
79	858.5
80	487.0
81	329.5
82	172.0
83	138.5
84	105.0
85	87.5
86	70.0
87	49.5
88	29.0
89	25.5
90	22.0
91	24.0
92	26.0
93	19.0
94	12.0
95	7.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1336622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.661874043145914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31109538116633	16.800396110758822
2	5.8304133828312255	2.409345338710621
3	1.7334296090781076	1.0744771273629456
4	0.8572291928385503	0.7084784643415107
5	0.5951496990951715	0.6148454059760311
6	0.4155223488128899	0.5151282259970443
7	0.35110990779533624	0.507821208311747
8	0.31087665788996244	0.5138635478621254
9	0.25004395842262755	0.46497390967601504
>10	5.5693364007344615	30.94284497455521
>50	2.3934137545627396	34.17596188694505
>100	0.37838523690128084	10.11624847217401
>500	0.0021788017479920144	0.2697336126312996
>1k	0.0018156681233266788	0.8858817146975673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3589	0.26851271339241756	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3006	0.22489529575302514	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2223	0.16631478458382398	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1621	0.12127587305909975	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1368	0.10234755974389169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.481546764904363E-5	0.0	0.0	0.02020017626524178	0.0
2	7.481546764904363E-5	0.0	0.0	0.07728437808146207	0.0
3	7.481546764904363E-5	0.0	0.0	0.11551508205012337	0.0
4	7.481546764904363E-5	0.0	0.0	0.18008083063124802	0.0
5	7.481546764904363E-5	0.0	0.0	0.3276917483028111	0.0
6	7.481546764904363E-5	0.0	0.0	0.48248495086868237	0.0
7	7.481546764904363E-5	0.0	0.0	0.577725041185915	0.0
8	7.481546764904363E-5	0.0	0.0	0.852148176522607	0.0
9	7.481546764904363E-5	0.0	0.0	0.9616032056931578	0.0
10	7.481546764904363E-5	0.0	0.0	1.174528026622336	0.0
11	7.481546764904363E-5	0.0	0.0	1.4024159410813228	0.0
12	7.481546764904363E-5	0.0	0.0	1.5842175274684989	0.0
13	7.481546764904363E-5	0.0	0.0	1.6640456314500285	0.0
14	7.481546764904363E-5	0.0	0.0	1.693971818509646	0.0
15	7.481546764904363E-5	0.0	0.0	1.752178252340602	0.0
16	7.481546764904363E-5	0.0	0.0	1.8768208214439086	0.0
17	7.481546764904363E-5	0.0	0.0	2.0188205790417935	0.0
18	7.481546764904363E-5	0.0	0.0	2.222169020111894	0.0
19	7.481546764904363E-5	0.0	0.0	2.3136683370466744	0.0
20	7.481546764904363E-5	0.0	0.0	2.4172877597405997	0.0
21	7.481546764904363E-5	0.0	0.0	2.5420051443115557	0.0
22	7.481546764904363E-5	0.0	0.0	2.6768226170151324	0.0
23	7.481546764904363E-5	0.0	0.0	2.8357306703017007	0.0
24	7.481546764904363E-5	0.0	0.0	2.9486272109841076	0.0
25	7.481546764904363E-5	0.0	0.0	3.043642854898393	0.0
26	7.481546764904363E-5	0.0	0.0	3.1414266711156933	0.0
27	7.481546764904363E-5	0.0	0.0	3.240706796685974	0.0
28	7.481546764904363E-5	0.0	0.0	3.3459721596681784	0.0
29	7.481546764904363E-5	0.0	0.0	3.455277557903431	0.0
30	7.481546764904363E-5	0.0	0.0	3.600344749674927	0.0
31	1.4963093529808726E-4	0.0	0.0	3.7400252277756914	0.0
32	1.4963093529808726E-4	0.0	0.0	3.8598047914818103	0.0
33	1.4963093529808726E-4	0.0	0.0	3.9800332479938234	0.0
34	2.244464029471309E-4	0.0	0.0	4.104825448032428	0.0
35	2.244464029471309E-4	0.0	0.0	4.26500536426903	0.0
36	2.244464029471309E-4	0.0	0.0	4.412691097408243	0.0
37	2.244464029471309E-4	0.0	0.0	4.562172401771032	0.0
38	2.244464029471309E-4	0.0	0.0	4.7026010345482865	0.0
39	2.244464029471309E-4	0.0	0.0	4.848940089269816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	50	2.1827873E-11	45.000004	1
CTCGTTA	20	7.0336985E-4	45.000004	37
ATTACGA	65	0.0	45.000004	12
AAACGCG	25	3.8911472E-5	45.000004	1
CGCTAAA	30	2.1657688E-6	45.000004	28
CCGTTAT	25	3.8911472E-5	45.000004	30
GCGTAAC	40	6.8157533E-9	45.000004	32
TACGTAG	40	6.8157533E-9	45.000004	1
ACGGTAC	20	7.0336985E-4	45.000004	37
GCGATAA	110	0.0	42.954544	9
ATTACGG	90	0.0	42.5	2
CGACGGT	170	0.0	41.02941	28
TGTTCGG	55	6.184564E-11	40.909092	2
CGAGGGA	720	0.0	40.625	4
ACGTAAG	50	1.0822987E-9	40.500004	1
ACGCTAG	45	1.9286745E-8	40.0	1
CGCGTAA	45	1.9286745E-8	40.0	31
CGGTCTA	170	0.0	39.705883	31
CATACGA	510	0.0	39.264706	18
CGCATGG	180	0.0	38.75	2
>>END_MODULE