FastQCFastQC Report
Sat 18 Jun 2016
SRR3549311_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549311_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955819
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103971.0877582471158243No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10060.10525005257271514No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9960.10420382938610762No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGA207.0326886E-445.00000412
CGACGTC453.8380676E-1045.00000418
CCGCTCG207.0326886E-445.00000419
TCCACCG207.0326886E-445.00000431
ACGTCGC453.8380676E-1045.00000420
CTTTCGT207.0326886E-445.00000439
CCAACGG453.8380676E-1045.0000042
CGCAATC207.0326886E-445.00000420
TCGACTA207.0326886E-445.00000429
AATCGGC207.0326886E-445.00000442
TGCGTAG700.045.0000041
CTATGCG253.8903105E-545.01
TGTTGCG551.8189894E-1245.01
ACTAACG253.8903105E-545.01
AATACGG253.8903105E-545.02
GTAATCG302.1651158E-644.9999961
CACGACG1200.043.12499626
CGGTCTA1150.043.04347631
CGTTTTT47150.042.232241
CGGGTCC700.041.7857176