##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955819
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.813306703465823	31.0	31.0	31.0	30.0	33.0
2	31.12319487266941	31.0	31.0	33.0	30.0	34.0
3	31.160982361723295	31.0	31.0	34.0	28.0	34.0
4	35.11790098334517	35.0	35.0	37.0	32.0	37.0
5	34.984413366965924	35.0	35.0	37.0	32.0	37.0
6	35.03570236624298	36.0	35.0	37.0	32.0	37.0
7	35.507829411216974	35.0	35.0	37.0	35.0	37.0
8	35.60034902005505	37.0	35.0	37.0	35.0	37.0
9	37.415809897062104	39.0	37.0	39.0	35.0	39.0
10	36.47635169420152	38.0	35.0	39.0	32.0	39.0
11	36.07187657914312	38.0	35.0	39.0	31.0	39.0
12	35.455167767119086	37.0	35.0	39.0	30.0	39.0
13	35.141996549555934	37.0	34.0	39.0	29.0	39.0
14	36.182977111775344	38.0	35.0	40.0	30.0	41.0
15	36.45080292398456	38.0	35.0	40.0	30.0	41.0
16	36.60034902005505	38.0	35.0	40.0	31.0	41.0
17	36.55427230469367	38.0	35.0	40.0	31.0	41.0
18	36.46203308366961	38.0	35.0	40.0	31.0	41.0
19	36.34829188371439	38.0	35.0	40.0	30.0	41.0
20	36.1637004495621	38.0	34.0	40.0	30.0	41.0
21	36.04158527922127	38.0	34.0	40.0	30.0	41.0
22	35.87975652294001	37.0	34.0	40.0	30.0	41.0
23	35.793981914986	37.0	34.0	40.0	30.0	41.0
24	35.628281086691096	37.0	34.0	40.0	29.0	41.0
25	35.45928674780476	37.0	34.0	40.0	29.0	41.0
26	35.20263669167489	36.0	34.0	40.0	29.0	41.0
27	35.19571487907229	36.0	34.0	40.0	28.0	41.0
28	35.18823647573442	36.0	34.0	40.0	28.0	41.0
29	35.17042034108968	36.0	34.0	40.0	29.0	41.0
30	35.0631761871233	36.0	34.0	40.0	27.0	41.0
31	34.957368497592114	36.0	33.0	40.0	27.0	41.0
32	34.73682569607844	36.0	33.0	40.0	26.0	41.0
33	34.52774426957405	36.0	33.0	40.0	25.0	41.0
34	34.3170736300492	36.0	33.0	40.0	24.0	41.0
35	34.07982055179903	35.0	33.0	40.0	23.0	41.0
36	33.922728047883545	35.0	33.0	40.0	23.0	41.0
37	33.813753440766504	35.0	33.0	40.0	23.0	41.0
38	33.75802531650867	35.0	33.0	40.0	23.0	41.0
39	33.71639714213674	35.0	33.0	40.0	22.0	41.0
40	33.49901184220025	35.0	33.0	40.0	21.0	41.0
41	33.420681112218944	35.0	33.0	40.0	21.0	41.0
42	33.33712972853647	35.0	32.0	40.0	21.0	41.0
43	33.251162615516115	35.0	32.0	40.0	20.0	41.0
44	33.0730033615151	35.0	32.0	39.0	19.0	41.0
45	32.936277684373295	35.0	32.0	39.0	20.0	41.0
46	32.83406586393449	35.0	31.0	39.0	19.0	41.0
47	32.77032994740636	35.0	31.0	39.0	19.0	41.0
48	32.6685910198479	35.0	31.0	39.0	18.0	41.0
49	32.54363326110906	35.0	31.0	39.0	18.0	41.0
50	32.395910732052826	35.0	31.0	39.0	18.0	41.0
51	32.0644651340892	35.0	31.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	4.0
14	12.0
15	48.0
16	136.0
17	330.0
18	657.0
19	1354.0
20	2258.0
21	3692.0
22	5549.0
23	8250.0
24	12024.0
25	16936.0
26	21008.0
27	23162.0
28	25376.0
29	29330.0
30	33853.0
31	40442.0
32	48860.0
33	61095.0
34	92204.0
35	110185.0
36	88174.0
37	89972.0
38	110228.0
39	130659.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.64555109283243	25.20477203319875	29.468026896305684	14.681649977663136
2	32.021229960902645	27.26405313139831	28.076445435799037	12.638271471900014
3	27.612863941813252	26.27715079947145	32.29021394217943	13.819771316535872
4	24.76357971540637	28.923363105357815	32.1220858760916	14.190971303144215
5	22.092153430722764	33.08513431936381	31.211976326061734	13.610735923851692
6	20.42897243097281	42.25747761867048	27.83884815012047	9.474701800236238
7	87.40336821092697	3.8136927598216817	7.4762062691785784	1.3067327600727752
8	87.56521893789515	3.5731660492206165	6.970985092365814	1.8906299205184247
9	83.20110815959926	5.961484339608232	8.613869362295581	2.223538138496933
10	44.579360736708516	26.855921466302718	16.65911642266998	11.905601374318778
11	36.21145844558436	27.183703190666854	21.930511948391903	14.674326415356884
12	33.24625269010137	25.046164598109055	25.437138202944283	16.270444508845294
13	21.386894380630643	37.14876979846603	25.96119139711598	15.503144423787349
14	17.01964493277493	39.722060348245854	26.905617067666576	16.352677651312643
15	16.331753187580496	30.969566413724774	37.47916708079668	15.219513317898054
16	17.21466093475857	27.352668235303963	38.453410112165585	16.97926071777188
17	17.644658664454255	28.939370320112907	27.298055384963053	26.117915630469785
18	20.008704576912574	31.228925141684776	30.746407008021393	18.015963273381256
19	24.627466078828732	33.41772867038634	23.878788766492402	18.076016484292527
20	25.933571104989543	31.269204734369165	25.587375852541115	17.209848308100174
21	21.146158425392255	34.36728083455131	26.805179641752257	17.68138109830418
22	22.081168087263382	31.336895374542667	24.95493393623688	21.627002601957066
23	19.277289947155264	35.51906794068751	24.425858870769463	20.777783241387752
24	18.85126786556869	32.422351930647956	32.603243919612396	16.123136284170958
25	18.189740944676764	33.91918344372732	29.198205936479603	18.692869675116313
26	17.148748874002294	38.532295340435795	26.082448664443792	18.23650712111812
27	18.112006561911826	37.903724449922	26.467668041752674	17.516600946413494
28	16.64185374009096	35.65047357292542	31.436705066545024	16.270967620438597
29	16.651165126451765	32.31009218272497	31.125767535485277	19.912975155337985
30	17.582303762532444	36.94444241012158	28.279412734000893	17.193841093345082
31	23.219040425017706	34.825840457241384	24.662723800217407	17.292395317523507
32	23.601016510448108	35.17486051229364	24.984960541692516	16.239162435565728
33	22.871694327064017	35.24202804087385	24.082383798606223	17.803893833455916
34	18.46699008912775	35.82676217986878	25.727779004183848	19.978468726819617
35	19.30124845812858	33.637330917255255	27.591520988806455	19.46989963580971
36	24.402528093708117	34.07548918780648	23.4325745774043	18.089408141081105
37	18.79707350450242	37.45238376721953	26.26051585080439	17.490026877473664
38	19.08279705676493	37.50992604248294	23.63501876401285	19.77225813673928
39	18.909856364018708	36.74806631799535	24.943006991909556	19.39907032607638
40	21.517253789681938	33.529674551353345	24.101843549877124	20.8512281090876
41	17.387078515911487	32.13024641694714	26.21343580740705	24.26923925973432
42	19.395931656516556	33.38676046406276	24.034885265934243	23.182422613486445
43	19.707810788444256	33.736094386071	25.419457031090616	21.13663779439413
44	18.418863822543805	36.05379261136261	25.529728954959047	19.997614611134534
45	17.628337582743175	39.40704254675833	22.439918017951097	20.5247018525474
46	20.505346723595157	37.70881307025702	23.458416290113504	18.327423916034313
47	19.972923743930597	34.635113970322834	24.999816910942343	20.392145374804223
48	20.43075101039004	32.4427532827868	27.242814800710175	19.88368090611298
49	19.16042681721121	32.670306825873936	27.24741818273125	20.921848174183605
50	18.568159871272698	36.087585620290035	25.990799513296974	19.353454995140293
51	17.611179522482814	36.54436666356287	23.773538713919688	22.07091510003463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	777.0
1	961.0
2	1145.0
3	7409.0
4	13673.0
5	10125.5
6	6578.0
7	6766.5
8	6955.0
9	8988.0
10	11021.0
11	13010.5
12	15000.0
13	15345.0
14	15690.0
15	15001.0
16	14312.0
17	12944.0
18	11576.0
19	10590.5
20	9605.0
21	9198.5
22	8792.0
23	8579.0
24	8366.0
25	8937.5
26	10222.0
27	10935.0
28	13172.0
29	15409.0
30	17026.5
31	18644.0
32	21556.0
33	24468.0
34	27091.5
35	29715.0
36	31381.5
37	33048.0
38	36925.5
39	40803.0
40	48492.0
41	56181.0
42	66202.0
43	76223.0
44	77156.5
45	78090.0
46	79175.0
47	80260.0
48	77342.0
49	74424.0
50	68900.5
51	63377.0
52	58487.0
53	53597.0
54	47352.5
55	41108.0
56	36422.5
57	31737.0
58	29561.0
59	27385.0
60	24339.0
61	21293.0
62	18576.5
63	15860.0
64	12910.5
65	9961.0
66	8314.0
67	6667.0
68	5632.5
69	4598.0
70	3918.5
71	3239.0
72	2604.5
73	1970.0
74	1733.5
75	1224.5
76	952.0
77	756.5
78	561.0
79	468.0
80	375.0
81	300.0
82	225.0
83	157.5
84	90.0
85	76.5
86	63.0
87	46.5
88	30.0
89	27.0
90	24.0
91	16.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	955819.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.470782783840068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.42083163001561	24.050233923886356
2	8.31359415701992	5.232706317371518
3	3.4647480845695013	3.271150031106382
4	1.9752317510828274	2.4864835754428687
5	1.2351154720551347	1.943502536700362
6	0.8864446290856854	1.6738263823114372
7	0.6562666112844528	1.4457256780413872
8	0.5510079227253096	1.3872520518610523
9	0.4770394739695345	1.3511525098151294
>10	5.194194057159772	37.685793775471375
>50	0.7794566407274656	15.928793605123442
>100	0.04437583610247231	2.074878073448069
>500	0.0010162405214305021	0.25287010174270524
>1k	3.387468404768341E-4	0.1072459200477072
>5k	0.0	0.0
>10k+	3.387468404768341E-4	1.1083855176302302
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10397	1.0877582471158243	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1006	0.10525005257271514	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.10420382938610762	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03693167848724497	0.0
2	0.0	0.0	0.0	0.12408206993165023	0.0
3	0.0	0.0	0.0	0.20118871878462344	0.0
4	0.0	0.0	0.0	0.3525772138867296	0.0
5	0.0	0.0	0.0	0.6551449594536204	0.0
6	0.0	0.0	0.0	1.2378912743940014	0.0
7	0.0	0.0	0.0	1.5831449259744785	0.0
8	0.0	0.0	0.0	2.2389176193400635	0.0
9	0.0	0.0	0.0	2.570570369494643	0.0
10	0.0	0.0	0.0	3.0017189446955963	0.0
11	0.0	0.0	0.0	3.4340183654018177	0.0
12	0.0	0.0	0.0	3.7416079822644246	0.0
13	0.0	0.0	0.0	3.9195705463063613	0.0
14	0.0	0.0	0.0	4.007348671662731	0.0
15	0.0	0.0	0.0	4.107786097577052	0.0
16	0.0	0.0	0.0	4.2952692926171165	0.0
17	0.0	0.0	0.0	4.502839972840046	0.0
18	0.0	0.0	0.0	4.754456649219152	0.0
19	0.0	0.0	0.0	4.902287985486792	0.0
20	0.0	0.0	0.0	5.05807061797265	0.0
21	0.0	0.0	0.0	5.254865199373522	0.0
22	0.0	0.0	0.0	5.470387175814668	0.0
23	0.0	0.0	0.0	5.717714337128682	0.0
24	0.0	0.0	0.0	5.911474871288393	0.0
25	0.0	0.0	0.0	6.073534842893896	0.0
26	0.0	0.0	0.0	6.230782187841004	0.0
27	0.0	0.0	0.0	6.385937086414897	0.0
28	0.0	0.0	0.0	6.556680710469242	0.0
29	0.0	0.0	0.0	6.745628617970557	0.0
30	0.0	0.0	0.0	6.967323311212688	0.0
31	0.0	0.0	0.0	7.167884296085346	0.0
32	0.0	0.0	0.0	7.370223860375239	0.0
33	0.0	0.0	0.0	7.574132759445042	0.0
34	0.0	0.0	0.0	7.773542898812432	0.0
35	0.0	0.0	0.0	8.00318888827278	0.0
36	0.0	0.0	0.0	8.23053318672259	0.0
37	0.0	0.0	0.0	8.454424948656596	0.0
38	0.0	0.0	0.0	8.673608706250869	0.0
39	0.0	0.0	0.0	8.906079498315057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGA	20	7.0326886E-4	45.000004	12
CGACGTC	45	3.8380676E-10	45.000004	18
CCGCTCG	20	7.0326886E-4	45.000004	19
TCCACCG	20	7.0326886E-4	45.000004	31
ACGTCGC	45	3.8380676E-10	45.000004	20
CTTTCGT	20	7.0326886E-4	45.000004	39
CCAACGG	45	3.8380676E-10	45.000004	2
CGCAATC	20	7.0326886E-4	45.000004	20
TCGACTA	20	7.0326886E-4	45.000004	29
AATCGGC	20	7.0326886E-4	45.000004	42
TGCGTAG	70	0.0	45.000004	1
CTATGCG	25	3.8903105E-5	45.0	1
TGTTGCG	55	1.8189894E-12	45.0	1
ACTAACG	25	3.8903105E-5	45.0	1
AATACGG	25	3.8903105E-5	45.0	2
GTAATCG	30	2.1651158E-6	44.999996	1
CACGACG	120	0.0	43.124996	26
CGGTCTA	115	0.0	43.043476	31
CGTTTTT	4715	0.0	42.23224	1
CGGGTCC	70	0.0	41.785717	6
>>END_MODULE