FastQCFastQC Report
Sat 18 Jun 2016
SRR3549310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534602
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62620.4080536842777476No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC16470.10732424433175507No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG253.8914186E-545.01
TCGTTCG207.0340274E-445.01
ACTACGG453.8562575E-1045.02
TAAATCG207.0340274E-445.022
CGTTTCG253.8914186E-545.042
CAACGGC302.1659798E-644.99999623
TCTACGC302.1659798E-644.99999629
CGTTTTT33250.042.0225521
CGCGAGG750.042.02
TACGTAG750.042.01
TCGCGGC2400.041.24999622
GTACCGA2100.040.71428742
CCGACCA2100.040.71428745
CGACGGT2000.040.528
ACGTTAG1000.040.51
CCTCGCG2400.040.31249620
GGGCGAT21050.039.8693587
TGTACCG2150.039.7674441
TCGTAAG800.039.3751
TCGATAG1200.039.3749961
Produced by FastQC (version 0.11.5)