##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549310_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1534602
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.406505400097224	31.0	31.0	33.0	30.0	34.0
2	31.805935350012575	31.0	31.0	34.0	30.0	34.0
3	31.927467186931857	33.0	31.0	34.0	30.0	34.0
4	35.67801618921388	37.0	35.0	37.0	33.0	37.0
5	35.56451249248991	37.0	35.0	37.0	33.0	37.0
6	35.628032545246256	37.0	35.0	37.0	33.0	37.0
7	35.96636782696751	37.0	35.0	37.0	35.0	37.0
8	35.978520163534256	37.0	35.0	37.0	35.0	37.0
9	37.73941973228238	39.0	38.0	39.0	35.0	39.0
10	37.23809235228418	39.0	37.0	39.0	34.0	39.0
11	36.887035205219334	39.0	37.0	39.0	33.0	39.0
12	36.335803029059	38.0	35.0	39.0	32.0	39.0
13	36.13916442178493	38.0	35.0	39.0	32.0	39.0
14	37.1998140234406	39.0	35.0	41.0	32.0	41.0
15	37.40449380360511	39.0	35.0	41.0	32.0	41.0
16	37.48543335666186	39.0	35.0	41.0	33.0	41.0
17	37.416047287830985	39.0	35.0	41.0	32.0	41.0
18	37.31719950840674	39.0	35.0	41.0	32.0	41.0
19	37.28317700615534	39.0	35.0	41.0	32.0	41.0
20	37.154225004268206	39.0	35.0	41.0	32.0	41.0
21	37.02687537224635	39.0	35.0	41.0	32.0	41.0
22	36.94180575810536	39.0	35.0	41.0	32.0	41.0
23	36.83648789718767	38.0	35.0	41.0	31.0	41.0
24	36.7877299781963	38.0	35.0	41.0	31.0	41.0
25	36.8124621237298	38.0	35.0	41.0	31.0	41.0
26	36.73080837898035	38.0	35.0	41.0	31.0	41.0
27	36.67680089039373	38.0	35.0	41.0	31.0	41.0
28	36.64375909845028	38.0	35.0	41.0	31.0	41.0
29	36.675836470954685	38.0	35.0	41.0	31.0	41.0
30	36.607728257880545	38.0	35.0	41.0	31.0	41.0
31	36.46254142767962	38.0	35.0	40.0	31.0	41.0
32	36.326891272134404	38.0	35.0	41.0	30.0	41.0
33	36.21075236445671	38.0	35.0	41.0	30.0	41.0
34	36.09461019860524	38.0	35.0	41.0	30.0	41.0
35	36.00349797537081	38.0	35.0	41.0	30.0	41.0
36	35.923292814684196	38.0	35.0	41.0	29.0	41.0
37	35.85997151052846	38.0	35.0	40.0	29.0	41.0
38	35.799084713821564	38.0	35.0	40.0	29.0	41.0
39	35.75097777795155	38.0	35.0	40.0	29.0	41.0
40	35.61041885778853	38.0	35.0	40.0	28.0	41.0
41	35.52769773530857	38.0	34.0	40.0	28.0	41.0
42	35.49734393673408	38.0	34.0	40.0	28.0	41.0
43	35.43441426506677	38.0	34.0	40.0	28.0	41.0
44	35.27377261335512	38.0	34.0	40.0	27.0	41.0
45	35.17639622521018	38.0	34.0	40.0	27.0	41.0
46	35.11825672063506	37.0	34.0	40.0	27.0	41.0
47	35.05994583611907	37.0	34.0	40.0	27.0	41.0
48	34.94868636949515	37.0	34.0	40.0	26.0	41.0
49	34.82335941175627	37.0	34.0	40.0	26.0	41.0
50	34.71397469832569	37.0	34.0	40.0	26.0	41.0
51	34.374072886650744	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	5.0
11	4.0
12	7.0
13	3.0
14	10.0
15	44.0
16	80.0
17	227.0
18	490.0
19	1013.0
20	1727.0
21	2870.0
22	4523.0
23	6647.0
24	10194.0
25	14558.0
26	19556.0
27	22725.0
28	25736.0
29	30458.0
30	36279.0
31	44245.0
32	55071.0
33	71948.0
34	124179.0
35	177591.0
36	107054.0
37	141676.0
38	229074.0
39	406530.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.86943455045673	24.222697481171014	30.39758843009458	12.510279538277677
2	30.512276147170407	27.0365866850167	29.58441341794159	12.866723749871303
3	29.534693686050197	25.937734995783924	31.41576773652061	13.111803581645272
4	26.32839003207346	29.339594240070067	30.31900127850739	14.013014449349082
5	23.650040857499206	34.07678342658227	28.66469612316418	13.608479592754344
6	22.286495130333467	42.12831730963468	25.124559983630935	10.460627576400917
7	89.43849936335285	4.221680930951479	5.041502617616815	1.2983170880788635
8	90.06367774836733	3.5407877742893596	4.772638117244732	1.622896360098579
9	84.94710680684634	7.089590656078905	5.941084398430342	2.0222181386444174
10	44.19302203437764	37.64311528331124	10.292310318897018	7.871552363414097
11	34.31717148811222	25.24739313515817	26.024076600968847	14.411358775760752
12	33.850405512308726	26.126122603776093	25.41616653699135	14.607305346923827
13	24.199694774280236	34.05971059597211	25.566694165653374	16.173900464094274
14	18.0035605323074	37.40715833812285	27.550140036309088	17.039141093260664
15	17.110104118201335	30.7673259907129	37.328115042206385	14.794454848879385
16	20.774572169200876	28.545055981941896	35.85828768631867	14.822084162538559
17	21.13981345000202	27.55131297887009	26.865988705866407	24.442884865261483
18	22.686012399306136	29.81137780349563	29.655571933309094	17.84703786388914
19	25.829172645415554	30.892896008215807	25.524142416079215	17.753788930289417
20	28.522053275051118	30.47226577314509	24.90202671441846	16.103654237385328
21	24.90723979246736	29.98894827453633	28.101618530407237	17.002193402589075
22	23.66359486042635	26.795742479157465	28.164631611323326	21.37603104909286
23	20.764732484383572	30.7534461704077	27.616280964054525	20.8655403811542
24	21.60201798251273	29.095882841283927	32.51481491618022	16.78728426002312
25	19.84319061228905	31.018270535291887	29.64253923818684	19.495999614232222
26	19.051324056660945	36.37216685498911	25.984196553894755	18.59231253445519
27	18.91408977702362	35.74679297954779	28.027462495161615	17.311654748266978
28	16.650571288190687	33.522959047362114	32.36168074849375	17.464788915953452
29	18.179241262555372	30.563690129427695	31.824277565127634	19.432791042889296
30	20.42692502681477	32.27123384434531	29.427630095620884	17.874211033219037
31	25.506483114188566	31.268367954687925	25.405544890466714	17.819604040656795
32	25.698454713339352	31.570140010243698	25.881759570233847	16.849645706183104
33	24.63844045557089	31.854643744762484	25.69070025974161	17.81621553992501
34	20.984203070242316	31.979236310131228	27.221194811423416	19.81536580820304
35	20.359220175654666	31.660521750916526	27.955065873757494	20.025192199671316
36	24.67239062636436	30.765371086444564	26.66065859421531	17.90157969297577
37	20.563637998647206	34.55918863653247	27.316724466669534	17.560448898150792
38	21.019065529694345	33.661105615658	24.815489618806698	20.504339235840956
39	20.966739258778496	32.65713194691522	27.46471071978272	18.911418074523556
40	22.943082310592583	30.003935873926917	26.43590976683205	20.617072048648446
41	18.73078492012913	30.296128898567837	27.848914571986743	23.124171609316292
42	21.91082769343452	30.511885166316738	25.455525276260556	22.121761863988187
43	21.86404031794563	30.59118911613565	25.925223608466556	21.619546957452158
44	19.85081473893557	33.202094093452246	26.52948451780983	20.41760664980236
45	19.71208169935918	34.72203216208502	24.376548447089213	21.18933769146658
46	22.26394856777197	33.06121065918069	25.42372550016226	19.251115272885087
47	21.106840731342718	31.632110475550014	26.47963445896721	20.78141433414006
48	21.69826443599057	29.934276118498477	28.526354064441467	19.84110538106949
49	21.461851346472898	28.761268394020078	28.33203658016867	21.44484367933836
50	20.158256016869522	32.48699011209421	27.072361433127284	20.28239243790898
51	19.113946156723372	32.80094773758929	26.071385284262625	22.01372082142471
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1020.0
1	1722.5
2	2425.0
3	6628.0
4	10831.0
5	8105.5
6	5380.0
7	5240.0
8	5100.0
9	5502.5
10	5905.0
11	6069.0
12	6233.0
13	6446.5
14	6660.0
15	6514.0
16	6368.0
17	6412.5
18	6457.0
19	7032.0
20	7607.0
21	8308.5
22	9010.0
23	9810.5
24	10611.0
25	12548.5
26	17658.0
27	20830.0
28	25179.5
29	29529.0
30	34624.0
31	39719.0
32	45486.0
33	51253.0
34	56079.5
35	60906.0
36	66822.5
37	72739.0
38	77173.0
39	81607.0
40	94499.5
41	107392.0
42	121327.5
43	135263.0
44	136647.0
45	138031.0
46	135948.5
47	133866.0
48	129720.0
49	125574.0
50	114609.5
51	103645.0
52	94096.0
53	84547.0
54	74227.5
55	63908.0
56	58558.0
57	53208.0
58	49624.5
59	46041.0
60	41167.0
61	36293.0
62	31404.5
63	26516.0
64	19985.5
65	13455.0
66	9593.5
67	5732.0
68	4386.5
69	3041.0
70	2337.0
71	1633.0
72	1214.5
73	796.0
74	587.5
75	347.5
76	316.0
77	241.5
78	167.0
79	114.5
80	62.0
81	47.0
82	32.0
83	24.0
84	16.0
85	10.5
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1534602.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.76417128531143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92315924012715	16.74168813509414
2	8.015944544517858	3.489207801608888
3	2.655281644042883	1.733700135350779
4	1.360442084019246	1.1843557816136365
5	0.8285892216816106	0.9016778872920729
6	0.6016980196918115	0.7857275257563165
7	0.47195397567733993	0.7190181015797732
8	0.37772465066401495	0.6576691196588836
9	0.34451038883385593	0.6748184800934355
>10	6.00088248443678	34.3895777312968
>50	2.269639710452262	33.53332442535134
>100	0.14774206766326736	4.421753177124345
>500	0.0018239761439012424	0.24420473689491956
>1k	3.039960239835404E-4	0.10896916869843742
>5k	3.039960239835404E-4	0.41430779258628725
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6262	0.4080536842777476	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1647	0.10732424433175507	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3032695122253197E-4	0.0	0.0	0.05610575250130001	0.0
2	1.3032695122253197E-4	0.0	0.0	0.20428749604131885	0.0
3	1.3032695122253197E-4	0.0	0.0	0.3135014811658006	0.0
4	1.3032695122253197E-4	0.0	0.0	0.49621986677979046	0.0
5	1.3032695122253197E-4	0.0	0.0	0.836894517275489	0.0
6	1.3032695122253197E-4	0.0	0.0	1.1410124579532674	0.0
7	1.3032695122253197E-4	0.0	0.0	1.3256857478355952	0.0
8	1.3032695122253197E-4	0.0	0.0	1.7255288341863233	0.0
9	1.3032695122253197E-4	0.0	0.0	1.8533144098600158	0.0
10	1.3032695122253197E-4	0.0	0.0	2.0947450869997564	0.0
11	1.3032695122253197E-4	0.0	0.0	2.4833800555453465	0.0
12	1.3032695122253197E-4	0.0	0.0	2.77850543658877	0.0
13	1.3032695122253197E-4	0.0	0.0	2.903163165433122	0.0
14	1.3032695122253197E-4	0.0	0.0	2.953339041653797	0.0
15	1.3032695122253197E-4	0.0	0.0	3.0467834656803525	0.0
16	1.3032695122253197E-4	0.0	0.0	3.258434434465744	0.0
17	1.3032695122253197E-4	0.0	0.0	3.5356398597160696	0.0
18	1.3032695122253197E-4	0.0	0.0	3.8431463011256337	0.0
19	1.3032695122253197E-4	0.0	0.0	4.034726919422756	0.0
20	1.3032695122253197E-4	0.0	0.0	4.225330085585709	0.0
21	1.3032695122253197E-4	0.0	0.0	4.46317677156683	0.0
22	1.3032695122253197E-4	0.0	0.0	4.738101475170761	0.0
23	1.3032695122253197E-4	0.0	0.0	5.016740496884534	0.0
24	1.3032695122253197E-4	0.0	0.0	5.232496764633436	0.0
25	1.3032695122253197E-4	0.0	0.0	5.41078403390586	0.0
26	1.3032695122253197E-4	0.0	0.0	5.586073783300165	0.0
27	1.3032695122253197E-4	0.0	0.0	5.762471311779862	0.0
28	1.9549042683379795E-4	0.0	0.0	5.952553170137925	0.0
29	1.9549042683379795E-4	0.0	0.0	6.181016315631023	0.0
30	1.9549042683379795E-4	0.0	0.0	6.439194006002859	0.0
31	2.6065390244506395E-4	0.0	0.0	6.686359068996391	0.0
32	2.6065390244506395E-4	0.0	0.0	6.904721875769743	0.0
33	2.6065390244506395E-4	0.0	0.0	7.126864164128549	0.0
34	2.6065390244506395E-4	0.0	0.0	7.363016599743777	0.0
35	2.6065390244506395E-4	0.0	0.0	7.642958890969776	0.0
36	2.6065390244506395E-4	0.0	0.0	7.903286976036783	0.0
37	2.6065390244506395E-4	0.0	0.0	8.16863264872586	0.0
38	2.6065390244506395E-4	0.0	0.0	8.431697599768539	0.0
39	2.6065390244506395E-4	0.0	0.0	8.757449814349258	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	25	3.8914186E-5	45.0	1
TCGTTCG	20	7.0340274E-4	45.0	1
ACTACGG	45	3.8562575E-10	45.0	2
TAAATCG	20	7.0340274E-4	45.0	22
CGTTTCG	25	3.8914186E-5	45.0	42
CAACGGC	30	2.1659798E-6	44.999996	23
TCTACGC	30	2.1659798E-6	44.999996	29
CGTTTTT	3325	0.0	42.022552	1
CGCGAGG	75	0.0	42.0	2
TACGTAG	75	0.0	42.0	1
TCGCGGC	240	0.0	41.249996	22
GTACCGA	210	0.0	40.714287	42
CCGACCA	210	0.0	40.714287	45
CGACGGT	200	0.0	40.5	28
ACGTTAG	100	0.0	40.5	1
CCTCGCG	240	0.0	40.312496	20
GGGCGAT	2105	0.0	39.869358	7
TGTACCG	215	0.0	39.76744	41
TCGTAAG	80	0.0	39.375	1
TCGATAG	120	0.0	39.374996	1
>>END_MODULE