FastQCFastQC Report
Sat 18 Jun 2016
SRR3549306_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549306_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228578
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.6517290721468234No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA49270.4010327386620955No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA39840.32427733526076485No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA38080.309951830490209No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA37460.3049053458551268No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA31010.25240562666757826No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29700.24174289300313045No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25140.20462681246123568No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA21230.17280140129483026No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA16080.1308830208582605No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA13910.11322032463547288No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG13250.10784826034651443No Hit
ATGAAGGGATAGTTGTTTTGTTGCTGATGTTTCCATTGAAATTTCAGCTC12850.10459246380775172No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC12400.10092969270164369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG204.091012E-580.000011
GATCGCG204.091012E-580.000019
AGTTACG404.1836756E-1180.000011
CGATTGG850.080.02
TACGGGT750.080.04
GCATGCG500.080.01
TCGCGAG351.2896635E-980.01
CGGGATA3550.077.746486
CAACGCG700.074.285711
GATTATC1350.074.074079
AGTAGCG600.073.333331
CGTTTTT42150.073.072361
CGGGACG1150.073.043486
CCCGTCA503.6379788E-1272.07
GCTACGG1000.072.02
CTACGGG1950.071.794873
ACGGGAT5350.071.028045
CGAAACG2650.070.9433980
GGACGTA850.070.5882348
TGCGAAG1250.070.41
Produced by FastQC (version 0.11.5)