##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549306_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1228578
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9105917572999	31.0	31.0	33.0	31.0	34.0
2	32.33890644305856	33.0	31.0	34.0	31.0	34.0
3	32.47148898971005	34.0	31.0	34.0	31.0	34.0
4	36.04048257416297	37.0	35.0	37.0	35.0	37.0
5	35.98980365918973	37.0	35.0	37.0	35.0	37.0
6	36.053260761628486	37.0	35.0	37.0	35.0	37.0
7	36.28680230315047	37.0	36.0	37.0	35.0	37.0
8	36.24170300949553	37.0	37.0	37.0	35.0	37.0
9	38.094384727709595	39.0	39.0	39.0	37.0	39.0
10-11	37.65785770215648	39.0	37.5	39.0	35.0	39.0
12-13	36.51344196298485	39.0	35.0	39.0	33.0	39.0
14-15	37.50329405214809	40.0	35.0	41.0	33.0	41.0
16-17	37.7241131617203	40.0	35.0	41.0	34.0	41.0
18-19	37.60585082916998	39.0	35.0	41.0	34.0	41.0
20-21	37.39132761615461	39.0	35.0	41.0	33.5	41.0
22-23	37.18518726527742	38.5	35.0	41.0	33.0	41.0
24-25	37.10129312099028	38.0	35.0	41.0	33.0	41.0
26-27	37.0733197241038	38.0	35.0	41.0	33.0	41.0
28-29	37.11459874749507	38.0	35.0	41.0	33.0	41.0
30-31	36.978077907955374	38.0	35.0	41.0	33.0	41.0
32-33	36.60268090426493	38.0	35.0	41.0	32.5	41.0
34-35	36.33197444525297	38.0	35.0	41.0	31.5	41.0
36-37	36.1224525426957	38.0	35.0	41.0	31.0	41.0
38-39	36.089901902850286	38.0	35.0	41.0	31.0	41.0
40-41	35.95049805547552	38.0	35.0	41.0	30.0	41.0
42-43	35.9311370543832	38.0	35.0	41.0	30.5	41.0
44-45	35.79117687277487	38.0	35.0	40.5	30.0	41.0
46-47	35.731629575004604	37.0	35.0	40.0	30.0	41.0
48-49	35.66751683653785	37.0	35.0	40.0	30.0	41.0
50-51	35.50556822603042	37.0	35.0	40.0	30.0	41.0
52-53	35.33786947186097	36.0	35.0	40.0	29.0	41.0
54-55	35.197068480796496	36.0	35.0	40.0	29.0	41.0
56-57	34.95051474143278	36.0	34.0	40.0	28.5	41.0
58-59	34.66600167022362	35.5	34.0	39.5	27.0	41.0
60-61	34.39776107011521	35.0	34.0	39.0	26.5	41.0
62-63	33.962736187690155	35.0	33.5	39.0	26.0	41.0
64-65	33.72556809579856	35.0	33.5	38.5	26.0	40.0
66-67	33.49833506704499	35.0	33.0	37.5	25.5	40.0
68-69	33.219823242805916	35.0	33.5	37.0	25.0	39.5
70-71	33.00169301420016	35.0	33.0	36.5	25.0	39.0
72-73	32.73736140481109	35.0	33.0	36.0	25.0	39.0
74-75	32.4641402499475	35.0	33.0	36.0	24.5	38.0
76-77	30.395794162031226	33.0	30.0	34.5	19.5	36.0
78-79	31.40580899218446	34.0	31.5	35.0	21.0	36.5
80-81	31.8257575017622	35.0	32.5	35.0	23.0	36.0
82-83	31.742100623647826	35.0	32.5	35.0	22.5	36.0
84-85	31.56639057512018	35.0	32.0	35.0	21.0	36.0
86	31.166817247256585	34.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	11.0
12	25.0
13	48.0
14	190.0
15	521.0
16	1468.0
17	3038.0
18	5959.0
19	8511.0
20	10235.0
21	10587.0
22	9127.0
23	8252.0
24	8472.0
25	9297.0
26	10625.0
27	12874.0
28	16074.0
29	20048.0
30	25302.0
31	31595.0
32	41221.0
33	58606.0
34	175428.0
35	154297.0
36	90619.0
37	161480.0
38	234723.0
39	119645.0
40	290.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.904364232470385	25.274015976193613	29.07499564537213	13.746624145963871
2	29.53072576588544	27.00968111100801	30.76027732874917	12.69931579435738
3	30.19596639366813	24.69749580409221	32.164339586090584	12.942198216149075
4	27.311656239978248	27.285365682927743	31.366506644266785	14.036471432827218
5	23.996034439815787	34.073457281507565	29.073611931843157	12.856896346833494
6	22.53117018211298	41.374011255288636	26.264022308717884	9.830796253880504
7	86.45230502255453	5.356924835053207	6.816498423380526	1.3742717190117355
8	87.37719542430355	4.982915207662843	6.091188349457665	1.548701018575947
9	82.0929562469782	8.833627168970956	6.941114035901669	2.132302548149161
10-11	45.84938848001511	27.84646151892676	17.09317601324458	9.210973987813553
12-13	33.4543268722051	29.906281896631715	23.393508592861014	13.245882638302167
14-15	14.146680145664337	36.69335605879318	35.359089939751485	13.800873855791004
16-17	18.067432430012584	25.659949958407196	35.19947451443864	21.07314309714157
18-19	24.39812531235298	28.76638683095416	30.198041963961586	16.63744589273127
20-21	26.946600053069485	29.591039396766018	27.08830859741913	16.37405195274537
22-23	20.762906384454222	27.47916697189759	28.74550903564934	23.01241760799884
24-25	17.690614678107536	28.299953279319666	36.43313652043256	17.57629552214023
26-27	17.004699742303703	39.50237591752416	27.283249415177547	16.209674924994587
28-29	14.643067025455444	30.670580134106256	38.30949276317824	16.37686007726005
30-31	23.524310218805805	31.237210824221172	29.481400448323182	15.757078508649839
32-33	26.986442863212588	31.991619579709223	26.02647125375841	14.995466303319773
34-35	19.054264360911557	31.107955701632296	29.19330315209942	20.64447678535673
36-37	23.339625160144493	32.68526703229262	28.927019692685363	15.048088114877526
38-39	19.41875078342604	34.67769242164518	26.887548043347675	19.016008751581097
40-41	19.523872314171342	30.30739603020728	28.05869875579735	22.110032899824024
42-43	21.71762802198965	27.14418620551565	27.000239301045596	24.137946471449105
44-45	17.832689499567795	37.142004821834675	26.425062145016433	18.6002435335811
46-47	21.10854988450062	33.55106472686309	26.12980209640739	19.210583292228904
48-49	21.09015463405661	28.098704355767403	31.401343667231547	19.409797342944447
50-51	18.671626872693473	34.552018675248945	27.505864503515447	19.270489948542135
52-53	22.83143601790037	28.095936928709452	29.513714228970407	19.558912824419778
54-55	22.34554908194677	29.20604145605733	31.295937254289104	17.152472207706797
56-57	23.352973925953417	26.242045682081233	28.94378704485999	21.461193347105354
58-59	25.413689647706533	28.462214039320255	27.487632042898376	18.636464270074836
60-61	24.62656013700392	31.85874238347097	26.019308501373136	17.495388978151976
62-63	24.35673599885396	26.773025400096696	30.850178010675755	18.02006059037359
64-65	18.387843506883566	34.58347780930474	30.011281335006814	17.017397348804877
66-67	17.81441634149399	30.75242271959941	25.28703916234867	26.14612177655794
68-69	16.92188041784893	35.61796646203985	23.024138475538386	24.436014644572833
70-71	19.482238815931915	31.972858052154606	28.436004877183212	20.108898254730263
72-73	23.150707565982785	33.11666007367868	25.245080084455363	18.487552275883175
74-75	22.78923275526666	34.70463413800345	22.702791357162507	19.803341749567384
76-77	28.76333452169907	29.413435695576513	24.621717139652507	17.201512643071908
78-79	18.124327474527462	32.91337627728968	30.26482649046296	18.6974697577199
80-81	15.428894217542558	30.990991210977242	31.172664657840205	22.407449913639997
82-83	18.50672077800514	28.22222113695671	32.28736799779908	20.98369008723907
84-85	16.06589081035148	28.0230477836979	29.618754364802236	26.292307041148383
86	17.80814893315687	28.117221698581613	32.008549721710786	22.066079646550726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2326.0
1	2228.5
2	4050.0
3	4150.5
4	2466.0
5	2790.5
6	3401.0
7	3821.0
8	3760.0
9	3573.5
10	3144.0
11	2896.5
12	2967.0
13	2956.0
14	2931.0
15	2893.0
16	2883.0
17	2992.0
18	3027.0
19	3106.5
20	3444.5
21	3657.0
22	4080.0
23	4753.0
24	5789.5
25	9982.0
26	14859.0
27	18327.0
28	24949.5
29	29575.0
30	26301.0
31	22968.0
32	24533.0
33	27591.5
34	28707.0
35	29781.5
36	31175.0
37	31918.5
38	33730.5
39	36357.0
40	43121.5
41	52161.5
42	60061.5
43	64128.0
44	68613.0
45	71149.5
46	71408.0
47	74714.0
48	72544.5
49	60427.5
50	51579.0
51	43440.0
52	29141.5
53	24274.5
54	25001.0
55	23537.5
56	24145.5
57	25651.0
58	22105.0
59	15711.0
60	10933.0
61	8915.0
62	8601.0
63	6894.0
64	5413.0
65	3949.5
66	2025.5
67	1334.5
68	840.5
69	417.5
70	193.0
71	128.0
72	115.0
73	91.5
74	53.0
75	20.5
76	17.5
77	16.0
78	26.5
79	40.0
80	27.0
81	9.5
82	5.0
83	4.0
84	2.5
85	2.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.5
91	1.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1228578.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.194729454139804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.62778280670257	11.586842928824277
2	7.947978664595309	2.2563881370241154
3	2.1975039412833306	0.9357892176276839
4	1.0287410785064772	0.5841080515103777
5	0.6298031235175756	0.446994247385209
6	0.4092384167161405	0.3485417164515683
7	0.3377322960925742	0.335581299967167
8	0.29021603603931184	0.3295630491864735
9	0.22206255179959225	0.28369060602220064
>10	2.1560683050423135	7.024215027433992
>50	0.9976495446493061	10.487605613086368
>100	2.1309748086230265	60.400517995787474
>500	0.015010930648658542	1.350656613237868
>1k	0.008660152297303006	2.9733130626104654
>5k	5.77343486486867E-4	0.6561924338447672
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8007	0.6517290721468234	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4927	0.4010327386620955	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3984	0.32427733526076485	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3808	0.309951830490209	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3746	0.3049053458551268	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	3101	0.25240562666757826	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2970	0.24174289300313045	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2514	0.20462681246123568	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2123	0.17280140129483026	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1608	0.1308830208582605	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1391	0.11322032463547288	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1325	0.10784826034651443	No Hit
ATGAAGGGATAGTTGTTTTGTTGCTGATGTTTCCATTGAAATTTCAGCTC	1285	0.10459246380775172	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	1240	0.10092969270164369	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.139491346906749E-5	0.0	0.0	0.04647649559083754	0.0
2	8.139491346906749E-5	0.0	0.0	0.14439457649412574	0.0
3	8.139491346906749E-5	0.0	0.0	0.2008012515281895	0.0
4	8.139491346906749E-5	0.0	0.0	0.30083560018167343	0.0
5	8.139491346906749E-5	0.0	0.0	0.44409064788723224	0.0
6	8.139491346906749E-5	0.0	0.0	0.5825433956981161	0.0
7	8.139491346906749E-5	0.0	0.0	0.6670313158790081	0.0
8	8.139491346906749E-5	0.0	0.0	0.8409722459624053	0.0
9	8.139491346906749E-5	0.0	0.0	0.9008789022756389	0.0
10	8.139491346906749E-5	0.0	0.0	1.0078318185739936	0.0
11	8.139491346906749E-5	0.0	0.0	1.2281678493347594	0.0
12	8.139491346906749E-5	0.0	0.0	1.366213622578298	0.0
13	8.139491346906749E-5	0.0	0.0	1.4107366402458779	0.0
14	8.139491346906749E-5	0.0	0.0	1.4298644449111086	0.0
15	8.139491346906749E-5	0.0	0.0	1.4584340595387513	0.0
16	8.139491346906749E-5	0.0	0.0	1.5193174548136137	0.0
17	8.139491346906749E-5	0.0	0.0	1.5949333294263774	0.0
18	8.139491346906749E-5	0.0	0.0	1.688456085002336	0.0
19	8.139491346906749E-5	0.0	0.0	1.7567464174028837	0.0
20	8.139491346906749E-5	0.0	0.0	1.8242228006687407	0.0
21	8.139491346906749E-5	0.0	0.0	1.9094432750708543	0.0
22	8.139491346906749E-5	0.0	0.0	2.0028846357333436	0.0
23	8.139491346906749E-5	0.0	0.0	2.0940469388186993	0.0
24	8.139491346906749E-5	0.0	0.0	2.169255838864118	0.0
25	8.139491346906749E-5	0.0	0.0	2.226069488465527	0.0
26	8.139491346906749E-5	0.0	0.0	2.285324985471008	0.0
27	8.139491346906749E-5	0.0	0.0	2.3471037247940303	0.0
28	8.139491346906749E-5	0.0	0.0	2.4198707774353765	0.0
29	8.139491346906749E-5	0.0	0.0	2.5053354365778975	0.0
30	8.139491346906749E-5	0.0	0.0	2.6024395683464947	0.0
31	8.139491346906749E-5	0.0	0.0	2.6959623239224535	0.0
32	8.139491346906749E-5	0.0	0.0	2.7854967287384276	0.0
33	8.139491346906749E-5	0.0	0.0	2.8697404641789124	0.0
34	8.139491346906749E-5	0.0	0.0	2.9679027298226077	0.0
35	8.139491346906749E-5	0.0	0.0	3.082506767987055	0.0
36	8.139491346906749E-5	0.0	0.0	3.1835992505156367	0.0
37	8.139491346906749E-5	0.0	0.0	3.2893312431119552	0.0
38	8.139491346906749E-5	0.0	0.0	3.3975864780258154	0.0
39	8.139491346906749E-5	0.0	0.0	3.51503933816168	0.0
40	8.139491346906749E-5	0.0	0.0	3.6499921046933936	0.0
41	8.139491346906749E-5	0.0	0.0	3.7865727694944886	0.0
42	8.139491346906749E-5	0.0	0.0	3.926897600315161	0.0
43	1.6278982693813498E-4	0.0	0.0	4.045327199412655	0.0
44	1.6278982693813498E-4	0.0	0.0	4.182640418434971	0.0
45	1.6278982693813498E-4	0.0	0.0	4.318162949360969	0.0
46	1.6278982693813498E-4	0.0	0.0	4.456534302258383	0.0
47	1.6278982693813498E-4	0.0	0.0	4.610370688714921	0.0
48	1.6278982693813498E-4	0.0	0.0	4.759974539671067	0.0
49	1.6278982693813498E-4	0.0	0.0	4.913403951560259	0.0
50	1.6278982693813498E-4	0.0	0.0	5.079693759777564	0.0
51	1.6278982693813498E-4	0.0	0.0	5.234425490282261	0.0
52	1.6278982693813498E-4	0.0	0.0	5.4143896439623695	0.0
53	1.6278982693813498E-4	0.0	0.0	5.572051591351953	0.0
54	1.6278982693813498E-4	0.0	0.0	5.75299248399369	0.0
55	1.6278982693813498E-4	0.0	0.0	5.915619521104887	0.0
56	1.6278982693813498E-4	0.0	0.0	6.09338601212133	0.0
57	1.6278982693813498E-4	0.0	0.0	6.280919892754062	0.0
58	1.6278982693813498E-4	0.0	0.0	6.462511944703552	0.0
59	1.6278982693813498E-4	0.0	0.0	6.643208652604882	0.0
60	1.6278982693813498E-4	0.0	0.0	6.8547540327109875	0.0
61	1.6278982693813498E-4	0.0	0.0	7.053357621575513	0.0
62	1.6278982693813498E-4	0.0	0.0	7.292414482434164	0.0
63	1.6278982693813498E-4	0.0	0.0	7.525773699349981	0.0
64	1.6278982693813498E-4	0.0	0.0	7.769551465189838	0.0
65	1.6278982693813498E-4	0.0	0.0	7.97010853197762	0.0
66	1.6278982693813498E-4	0.0	0.0	8.181979491737602	0.0
67	1.6278982693813498E-4	0.0	0.0	8.39507137519962	0.0
68	1.6278982693813498E-4	0.0	0.0	8.641535173183957	0.0
69	1.6278982693813498E-4	0.0	0.0	8.878231581552006	0.0
70	1.6278982693813498E-4	0.0	0.0	9.100195510582152	0.0
71	1.6278982693813498E-4	0.0	0.0	9.301810711245032	0.0
72	1.6278982693813498E-4	0.0	0.0	9.555355866701179	0.0
73	1.6278982693813498E-4	0.0	0.0	9.805563830705092	0.0
74	1.6278982693813498E-4	0.0	0.0	10.08409722459624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACG	20	4.091012E-5	80.00001	1
GATCGCG	20	4.091012E-5	80.00001	9
AGTTACG	40	4.1836756E-11	80.00001	1
CGATTGG	85	0.0	80.0	2
TACGGGT	75	0.0	80.0	4
GCATGCG	50	0.0	80.0	1
TCGCGAG	35	1.2896635E-9	80.0	1
CGGGATA	355	0.0	77.74648	6
CAACGCG	70	0.0	74.28571	1
GATTATC	135	0.0	74.07407	9
AGTAGCG	60	0.0	73.33333	1
CGTTTTT	4215	0.0	73.07236	1
CGGGACG	115	0.0	73.04348	6
CCCGTCA	50	3.6379788E-12	72.0	7
GCTACGG	100	0.0	72.0	2
CTACGGG	195	0.0	71.79487	3
ACGGGAT	535	0.0	71.02804	5
CGAAACG	265	0.0	70.94339	80
GGACGTA	85	0.0	70.588234	8
TGCGAAG	125	0.0	70.4	1
>>END_MODULE