FastQCFastQC Report
Sat 18 Jun 2016
SRR3549305_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549305_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072828
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192530.9288276692518628No Hit
GTTTAAGGGCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACA39220.18921010329848884No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC37050.17874131379931185No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG30700.1481068376150843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTCGC204.091676E-580.000019
CGACGTG304.0618033E-880.03
CGTTTTT81150.074.282191
TAGTGCG1300.073.846151
CGGGAAC5700.072.982456
GCGCGAC850.070.5882349
CGGGTCA403.7161954E-970.000016
TCGCGTG351.1819611E-768.571431
TTTGCGG5750.068.173912
TAGGGTA9100.068.1318745
ACGGGAT9750.068.102565
TAAGGGA24450.068.057264
ATACCGG2000.068.02
GGCGATA4850.067.628878
CGTAAGG3550.067.605642
CCGGGAT2200.067.272735
TCGCACG303.7966602E-666.6666641
GGCGCGT303.7966602E-666.6666641
CGGGATA6250.066.566
AGTCACG1150.066.086961
Produced by FastQC (version 0.11.5)