##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2072828
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73041950417497	31.0	31.0	33.0	30.0	34.0
2	32.125287771103054	33.0	31.0	34.0	30.0	34.0
3	32.23688361986619	33.0	31.0	34.0	30.0	34.0
4	35.879766676250995	37.0	35.0	37.0	35.0	37.0
5	35.821164611825004	37.0	35.0	37.0	35.0	37.0
6	35.86506888174031	37.0	35.0	37.0	35.0	37.0
7	36.182215311641876	37.0	35.0	37.0	35.0	37.0
8	36.1682763837617	37.0	35.0	37.0	35.0	37.0
9	37.978236978659105	39.0	39.0	39.0	35.0	39.0
10-11	37.38224975733635	39.0	37.0	39.0	34.5	39.0
12-13	36.552395326577994	39.0	35.0	39.0	33.0	39.0
14-15	37.646269251476724	40.0	35.0	41.0	33.0	41.0
16-17	37.846846433954	40.0	35.0	41.0	33.0	41.0
18-19	37.748040117173254	39.0	36.0	41.0	33.5	41.0
20-21	37.514477805201395	39.0	35.0	41.0	33.0	41.0
22-23	37.28372011570666	39.0	35.0	41.0	33.0	41.0
24-25	37.260703251789344	39.0	35.0	41.0	33.0	41.0
26-27	37.17515297940784	39.0	35.0	41.0	33.0	41.0
28-29	37.151128796021666	39.0	35.0	41.0	33.0	41.0
30-31	37.00079914976062	39.0	35.0	41.0	32.5	41.0
32-33	36.65634654684325	39.0	35.0	41.0	31.5	41.0
34-35	36.38343702420075	38.0	35.0	41.0	31.0	41.0
36-37	36.20745980853211	38.0	35.0	41.0	30.0	41.0
38-39	36.183837974014246	38.0	35.0	41.0	30.0	41.0
40-41	36.08494867880982	38.0	35.0	41.0	30.0	41.0
42-43	35.983610072808744	38.0	35.0	41.0	30.0	41.0
44-45	35.7953633876038	38.0	35.0	40.0	29.5	41.0
46-47	35.69859462531382	38.0	35.0	40.0	29.0	41.0
48-49	35.62277936230116	38.0	35.0	40.0	29.0	41.0
50-51	35.47794824269066	37.5	35.0	40.0	29.0	41.0
52-53	35.247575052054486	37.0	34.5	40.0	28.0	41.0
54-55	35.10038411291241	37.0	34.0	40.0	28.0	41.0
56-57	34.81974867186278	36.0	34.0	40.0	27.0	41.0
58-59	34.50313725982089	36.0	34.0	39.5	26.0	41.0
60-61	34.0713725403169	35.5	33.5	39.0	25.5	40.5
62-63	33.56320230139693	35.0	33.0	39.0	23.5	40.0
64-65	33.144915304115926	35.0	33.0	38.0	22.5	40.0
66-67	32.69003988753529	35.0	32.0	37.5	20.5	40.0
68-69	32.13834167620276	35.0	32.0	37.0	18.5	39.5
70-71	31.71739358017163	35.0	31.5	36.0	16.5	39.0
72-73	31.334343949425616	35.0	31.0	36.0	13.0	38.5
74-75	30.96839076855388	34.5	31.0	35.5	11.5	37.5
76-77	29.017351897986714	32.5	28.0	34.5	10.0	36.0
78-79	29.85015302765111	34.0	29.0	35.0	10.0	36.0
80-81	30.0340727740073	34.0	30.0	35.0	10.0	36.0
82-83	29.749482832150086	34.0	29.5	35.0	10.0	36.0
84-85	29.46583628742954	34.0	29.0	35.0	10.0	36.0
86	29.042345529875128	34.0	29.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	3.0
11	7.0
12	29.0
13	77.0
14	294.0
15	879.0
16	2271.0
17	4827.0
18	9618.0
19	14728.0
20	18087.0
21	18750.0
22	17077.0
23	16061.0
24	17203.0
25	19733.0
26	24516.0
27	30040.0
28	38052.0
29	49015.0
30	62159.0
31	75448.0
32	90385.0
33	110881.0
34	244086.0
35	220568.0
36	170432.0
37	279804.0
38	377098.0
39	160550.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.295146534107026	24.611738166408404	31.559058445756232	14.534056853728336
2	29.647853078017082	25.540807052008173	31.400145115754903	13.411194754219839
3	27.10017425468973	24.22612971264379	35.50120897633571	13.17248705633077
4	25.90325873637369	26.60915425688962	33.30474115556139	14.182845851175301
5	23.391279932536612	30.873955774429913	31.74421611440988	13.9905481786236
6	21.91320263909982	39.50790900161518	27.49388757774403	11.085000781540968
7	87.07572456566585	3.3710949485437283	7.543414118296357	2.009766367494071
8	87.54165806328359	2.805539099240265	7.217337859195264	2.43546497828088
9	83.6617413504642	4.910827140505628	8.409332564013994	3.018098945016181
10-11	40.16360740013161	27.5158141437688	21.165094257700108	11.155484198399481
12-13	28.33814479541959	27.383169274054573	28.363665485028182	15.91502044549765
14-15	14.03013178131519	32.78851404940497	36.2475564783957	16.933797690884145
16-17	17.215586628509456	26.163724148843993	35.36615194314241	21.254537279504138
18-19	21.304469063520948	28.75692049702146	31.336946432603185	18.601664006854403
20-21	24.31989050707536	28.490279946044726	30.48024245137561	16.709587095504304
22-23	18.78525376924665	26.743463519404408	31.243595705963063	23.227687005385878
24-25	16.773268211351837	27.80315105739598	36.156207847443206	19.26737288380898
26-27	16.37842117146237	36.04237785286575	29.89830801204924	17.680892963622643
28-29	14.58929539739911	28.80743120027325	37.80825037099074	18.7950230313369
30-31	21.445701235220675	30.072803918125384	30.516352538657333	17.96514230799661
32-33	24.459144704722245	29.083044999392133	28.504149886049397	17.953660409836225
34-35	17.39309773893444	28.57716607456094	31.046932017514234	22.982804168990384
36-37	21.20750009166221	29.482378663352677	31.666110260957492	17.64401098402762
38-39	17.80919593907454	31.037114512154407	30.082380207137305	21.07130934163375
40-41	18.457754333692908	27.379478663931593	30.50407462654885	23.658692375826647
42-43	20.205246166107365	26.01602737902035	29.577442026062943	24.201284428809338
44-45	17.624641311290663	32.265146939350494	28.86421352857063	21.245998220788216
46-47	19.15474897097106	30.103366029405237	28.926085521808854	21.815799477814853
48-49	18.96445339410699	25.67316246210491	33.6091561866204	21.753227957167695
50-51	16.86485805865224	30.338214265727785	29.62267009129556	23.174257584324412
52-53	20.36881497162331	24.819208347243478	32.787573305648124	22.024403375485086
54-55	19.499447132130594	26.64550556051925	32.6807144635252	21.17433284382496
56-57	21.077918669566408	24.599460254299924	31.407671065809613	22.91495001032406
58-59	21.832829351977107	27.37807960911373	29.842225211160788	20.946865827748372
60-61	22.163826424575507	29.632535839924973	28.505404211058515	19.698233524441005
62-63	20.367247065361912	27.067151736661216	33.20627664234563	19.359324555631243
64-65	17.27314084912014	32.83106461317581	30.79744194887371	19.098352588830334
66-67	16.281138618351353	30.67321552970145	26.925388888996093	26.1202569629511
68-69	16.013291985635085	34.14306445107843	26.21671938047923	23.626924182807258
70-71	16.753078403031992	32.20993251731451	29.286149164330084	21.750839915323414
72-73	20.167761145642572	32.34342164424641	27.615822441611172	19.872994768499847
74-75	19.242189897087457	33.77906898208631	25.644047648912498	21.33469347191373
76-77	23.206508210039615	29.631016176933155	27.412211722342615	19.75026389068461
78-79	16.70478689018095	31.089940892346107	30.90041720779534	21.3048550096776
80-81	14.582806677640402	30.577259666503924	31.18782648632689	23.65210716952878
82-83	16.679555660189845	29.09966480576295	31.342783868222547	22.87799566582466
84-85	15.071703971578925	28.663280310763845	29.653690513636445	26.611325204020787
86	16.162701391528866	28.541827879592518	31.884652272161514	23.410818456717102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1519.0
1	1739.5
2	7222.5
3	7858.0
4	3376.5
5	4220.0
6	5709.0
7	6500.0
8	6930.0
9	7598.0
10	7784.0
11	7230.0
12	6445.0
13	6010.0
14	5858.0
15	5870.5
16	6071.0
17	6227.0
18	6166.5
19	6356.5
20	6781.0
21	6987.0
22	7417.5
23	8284.0
24	9610.0
25	11070.0
26	13111.0
27	17299.0
28	23522.0
29	27028.0
30	30079.0
31	36996.0
32	42040.0
33	43189.5
34	45788.0
35	52762.0
36	57109.0
37	61227.0
38	67612.5
39	75837.0
40	91197.5
41	107144.5
42	118867.0
43	124046.0
44	128123.0
45	130601.5
46	127559.0
47	127642.0
48	118944.0
49	97172.0
50	85625.0
51	71434.5
52	52165.0
53	45097.0
54	41139.0
55	37893.0
56	33038.0
57	29460.0
58	24717.0
59	16065.5
60	11277.0
61	10009.5
62	9334.5
63	7916.0
64	6785.0
65	4726.0
66	1924.0
67	902.5
68	629.0
69	411.5
70	165.5
71	142.0
72	148.5
73	135.0
74	74.0
75	44.0
76	38.0
77	19.0
78	32.0
79	47.0
80	25.5
81	5.0
82	5.0
83	3.0
84	2.0
85	1.5
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2072828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.191152969573512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09694717542989	14.41197000791656
2	10.650207138001502	4.08779508686061
3	4.051349319548625	2.3325019357390566
4	2.12317295601594	1.6298454791905388
5	1.243738372598095	1.193438668132923
6	0.8154312530344533	0.9389439547893119
7	0.6209681615861867	0.8341966484764747
8	0.45329285973301703	0.6959370088921405
9	0.35054278046152065	0.6054588109994999
>10	2.475928413100604	10.419703836584295
>50	0.7999492013625418	11.112741320696133
>100	1.30371524883238	48.119362151492304
>500	0.011195056775749156	1.445425610421241
>1k	0.0033076304110167955	1.232581537864448
>5k	0.0	0.0
>10k+	2.5443310853975356E-4	0.9400979419444684
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19253	0.9288276692518628	No Hit
GTTTAAGGGCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACA	3922	0.18921010329848884	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	3705	0.17874131379931185	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTG	3070	0.1481068376150843	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18810050809811524	0.0
2	0.0	0.0	0.0	0.5519030040119103	0.0
3	0.0	0.0	0.0	0.802044356791784	0.0
4	0.0	0.0	0.0	1.1669564479059527	0.0
5	0.0	0.0	0.0	1.9417433573842113	0.0
6	0.0	0.0	0.0	2.529201651077658	0.0
7	0.0	0.0	0.0	2.923011460671122	0.0
8	0.0	0.0	0.0	3.707302294256928	0.0
9	0.0	0.0	0.0	3.954114861435681	0.0
10	0.0	0.0	0.0	4.430275932204698	0.0
11	0.0	0.0	0.0	5.375072123688025	0.0
12	0.0	0.0	0.0	6.090857514468157	0.0
13	0.0	0.0	0.0	6.343893463422918	0.0
14	0.0	0.0	0.0	6.438209055454673	0.0
15	0.0	0.0	0.0	6.592394545037022	0.0
16	0.0	0.0	0.0	6.918326074329371	0.0
17	4.824326958146069E-5	0.0	0.0	7.3474499572564635	0.0
18	4.824326958146069E-5	0.0	0.0	7.829110760757767	0.0
19	4.824326958146069E-5	0.0	0.0	8.159239454503702	0.0
20	4.824326958146069E-5	0.0	0.0	8.462207187475276	0.0
21	4.824326958146069E-5	0.0	0.0	8.83821523059318	0.0
22	4.824326958146069E-5	0.0	0.0	9.257400999986492	0.0
23	4.824326958146069E-5	0.0	0.0	9.68666961272233	0.0
24	4.824326958146069E-5	0.0	0.0	9.99118113032051	0.0
25	4.824326958146069E-5	0.0	0.0	10.239103292699635	0.0
26	4.824326958146069E-5	0.0	0.0	10.481815181963965	0.0
27	4.824326958146069E-5	0.0	0.0	10.713431119224557	0.0
28	9.648653916292138E-5	0.0	0.0	10.969458150893368	0.0
29	9.648653916292138E-5	0.0	0.0	11.265189393427724	0.0
30	9.648653916292138E-5	0.0	0.0	11.62209310179137	0.0
31	9.648653916292138E-5	0.0	0.0	11.949471929171162	0.0
32	1.4472980874438208E-4	0.0	0.0	12.215871263799988	0.0
33	1.4472980874438208E-4	0.0	0.0	12.485309924412444	0.0
34	1.4472980874438208E-4	0.0	0.0	12.769655755325575	0.0
35	1.4472980874438208E-4	0.0	0.0	13.120384325182794	0.0
36	1.4472980874438208E-4	0.0	0.0	13.44260112271737	0.0
37	1.4472980874438208E-4	0.0	0.0	13.760041836563381	0.0
38	1.4472980874438208E-4	0.0	0.0	14.044918343441907	0.0
39	1.4472980874438208E-4	0.0	0.0	14.359319731304286	0.0
40	1.4472980874438208E-4	0.0	0.0	14.700785593401864	0.0
41	1.4472980874438208E-4	0.0	0.0	15.064202143159008	0.0
42	1.4472980874438208E-4	0.0	0.0	15.423903961158379	0.0
43	1.4472980874438208E-4	0.0	0.0	15.73077939896605	0.0
44	1.4472980874438208E-4	0.0	0.0	16.063513229269386	0.0
45	1.4472980874438208E-4	0.0	0.0	16.374923534417714	0.0
46	1.4472980874438208E-4	0.0	0.0	16.742633735167608	0.0
47	1.4472980874438208E-4	0.0	0.0	17.104602986837307	0.0
48	1.4472980874438208E-4	0.0	0.0	17.431837084408354	0.0
49	1.4472980874438208E-4	0.0	0.0	17.795832553400476	0.0
50	1.4472980874438208E-4	0.0	0.0	18.134596792401492	0.0
51	1.4472980874438208E-4	0.0	0.0	18.475773194881583	0.0
52	1.4472980874438208E-4	0.0	0.0	18.8600790803675	0.0
53	1.4472980874438208E-4	0.0	0.0	19.22079400702808	0.0
54	1.4472980874438208E-4	0.0	0.0	19.598152861694263	0.0
55	1.4472980874438208E-4	0.0	0.0	19.95770994988489	0.0
56	1.4472980874438208E-4	0.0	0.0	20.310223520716626	0.0
57	1.9297307832584276E-4	0.0	0.0	20.675521557987445	0.0
58	1.9297307832584276E-4	0.0	0.0	21.022294179738985	0.0
59	1.9297307832584276E-4	0.0	0.0	21.382044241007936	0.0
60	1.9297307832584276E-4	0.0	0.0	21.76847283035544	0.0
61	2.4121634790730346E-4	0.0	0.0	22.147471956187392	0.0
62	2.8945961748876416E-4	0.0	0.0	22.555079340881154	0.0
63	3.3770288707022484E-4	0.0	0.0	22.975615921822747	0.0
64	3.3770288707022484E-4	0.0	0.0	23.37801303340171	0.0
65	3.3770288707022484E-4	0.0	0.0	23.76579243429749	0.0
66	3.3770288707022484E-4	0.0	0.0	24.097271939591707	0.0
67	3.3770288707022484E-4	0.0	0.0	24.43979915362008	0.0
68	3.3770288707022484E-4	0.0	0.0	24.8501081614104	0.0
69	3.3770288707022484E-4	0.0	0.0	25.235957831522924	0.0
70	3.3770288707022484E-4	0.0	0.0	25.606996817873938	0.0
71	3.3770288707022484E-4	0.0	0.0	25.957628901191995	0.0
72	3.3770288707022484E-4	0.0	0.0	26.33334748469241	0.0
73	3.3770288707022484E-4	0.0	0.0	26.704000524886773	0.0
74	3.3770288707022484E-4	0.0	0.0	27.094047359452883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTCGC	20	4.091676E-5	80.00001	9
CGACGTG	30	4.0618033E-8	80.0	3
CGTTTTT	8115	0.0	74.28219	1
TAGTGCG	130	0.0	73.84615	1
CGGGAAC	570	0.0	72.98245	6
GCGCGAC	85	0.0	70.588234	9
CGGGTCA	40	3.7161954E-9	70.00001	6
TCGCGTG	35	1.1819611E-7	68.57143	1
TTTGCGG	575	0.0	68.17391	2
TAGGGTA	910	0.0	68.131874	5
ACGGGAT	975	0.0	68.10256	5
TAAGGGA	2445	0.0	68.05726	4
ATACCGG	200	0.0	68.0	2
GGCGATA	485	0.0	67.62887	8
CGTAAGG	355	0.0	67.60564	2
CCGGGAT	220	0.0	67.27273	5
TCGCACG	30	3.7966602E-6	66.666664	1
GGCGCGT	30	3.7966602E-6	66.666664	1
CGGGATA	625	0.0	66.56	6
AGTCACG	115	0.0	66.08696	1
>>END_MODULE