FastQCFastQC Report
Sat 18 Jun 2016
SRR3549304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107835
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114831.0365261974933089No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14960.13503816001480365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12440.11229109027968967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACG304.0599843E-880.01
CGTGCGT304.0599843E-880.05
CGTTAAG251.284774E-680.02
GTTCGCG150.001309778580.01
GCGATAT1000.080.09
ACGTCGG204.0908355E-580.02
CCTGGCG150.001309778580.01
TACGTCG150.001309778580.01
CTTACCG150.001309778580.01
TCGCGAG950.075.7894741
GGAACGT850.075.294118
CGTTTTT50150.075.054831
TACGCGG1150.073.043482
ACTACGG550.072.727272
TACGAGG1500.072.02
AATGCGG2300.071.304352
GCGTACG850.070.5882341
CGCGAGG1650.070.3030242
GTACGCG403.7143764E-970.01
GTAGTCG750.069.3333361
Produced by FastQC (version 0.11.5)