##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1107835
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79293667378265	31.0	31.0	33.0	30.0	34.0
2	32.18386673105652	33.0	31.0	34.0	30.0	34.0
3	32.28116100321799	34.0	31.0	34.0	30.0	34.0
4	35.902869109569565	37.0	35.0	37.0	35.0	37.0
5	35.86558467641842	37.0	35.0	37.0	35.0	37.0
6	35.90076590828057	37.0	35.0	37.0	35.0	37.0
7	36.21972947234922	37.0	35.0	37.0	35.0	37.0
8	36.21913552108391	37.0	36.0	37.0	35.0	37.0
9	38.03593495421249	39.0	39.0	39.0	35.0	39.0
10-11	37.44168626194334	39.0	37.5	39.0	34.5	39.0
12-13	36.33319086326033	38.5	35.0	39.0	33.0	39.0
14-15	37.32606164275366	39.0	35.0	41.0	33.0	41.0
16-17	37.61836600215736	39.5	35.0	41.0	33.0	41.0
18-19	37.48373629647014	39.0	35.0	41.0	33.0	41.0
20-21	37.22584184467904	39.0	35.0	41.0	33.0	41.0
22-23	36.96896469239553	38.0	35.0	41.0	32.5	41.0
24-25	36.92412182319569	38.0	35.0	41.0	33.0	41.0
26-27	36.836966244973304	38.0	35.0	41.0	33.0	41.0
28-29	36.85584811817644	38.0	35.0	41.0	33.0	41.0
30-31	36.72862700672934	38.0	35.0	41.0	32.0	41.0
32-33	36.304149534903665	38.0	35.0	41.0	31.0	41.0
34-35	35.997556043995715	38.0	35.0	41.0	30.0	41.0
36-37	35.76277017786945	38.0	35.0	41.0	30.0	41.0
38-39	35.7458005930486	38.0	35.0	41.0	30.0	41.0
40-41	35.64425433390352	38.0	35.0	40.0	29.0	41.0
42-43	35.54165782810617	38.0	35.0	40.0	29.0	41.0
44-45	35.36071075566308	37.0	35.0	40.0	28.5	41.0
46-47	35.26772263017507	37.0	35.0	40.0	28.0	41.0
48-49	35.19387137976323	37.0	35.0	40.0	28.0	41.0
50-51	35.015998772380364	36.0	34.0	40.0	27.5	41.0
52-53	34.78804154048211	36.0	34.0	40.0	27.0	41.0
54-55	34.65815802894836	36.0	34.0	40.0	27.0	41.0
56-57	34.398298483077355	35.5	34.0	39.5	25.5	41.0
58-59	34.123080603158414	35.0	34.0	39.0	25.5	41.0
60-61	33.753653296745455	35.0	33.5	39.0	25.0	40.0
62-63	33.301917253020534	35.0	32.5	38.0	23.5	40.0
64-65	33.052186020481386	35.0	32.5	37.5	23.0	40.0
66-67	32.7581056745815	35.0	33.0	37.0	22.5	40.0
68-69	32.40621572707127	35.0	32.5	36.5	21.0	39.0
70-71	32.16599448473825	35.0	32.0	36.0	20.0	39.0
72-73	31.92351342934643	35.0	32.0	36.0	20.0	38.5
74-75	31.646172038254797	35.0	32.0	35.0	19.5	37.5
76-77	29.620797320900678	32.5	28.5	34.5	16.0	35.5
78-79	30.550247103584923	34.0	30.5	35.0	17.0	36.0
80-81	30.908086041693934	34.0	31.0	35.0	18.0	36.0
82-83	30.771392400492854	34.0	31.0	35.0	17.5	36.0
84-85	30.575016586405013	34.0	31.0	35.0	16.0	36.0
86	30.221935577048928	34.0	30.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	5.0
12	11.0
13	49.0
14	204.0
15	705.0
16	1471.0
17	3133.0
18	6031.0
19	9814.0
20	11641.0
21	11716.0
22	10371.0
23	9265.0
24	9080.0
25	10330.0
26	11870.0
27	14634.0
28	17587.0
29	21673.0
30	27269.0
31	34049.0
32	42916.0
33	58204.0
34	151858.0
35	129506.0
36	86126.0
37	149597.0
38	200650.0
39	77953.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.74194261780861	24.698984957146145	28.78181317614988	15.777259248895367
2	29.762554893102312	25.302414168174863	32.43398159473207	12.501049343990756
3	28.45216119729021	24.495163991027542	34.53691208528346	12.515762726398789
4	24.99758538049439	27.05646598997143	33.950001579657616	13.99594704987656
5	24.207034441049437	30.423122576918043	31.97272156954781	13.397121412484712
6	21.342167380521467	40.41504375651609	27.757111844272835	10.485677018689605
7	87.7780535910131	3.608028271358099	7.434410358943344	1.179507778685454
8	89.03509999232739	2.765845094260427	6.842986545830381	1.356068367581815
9	85.0933577653712	5.060230088415694	8.04632458804786	1.8000875581652505
10-11	45.33725690197548	26.379379600752817	19.30328072321239	8.980082774059314
12-13	31.07746189640154	27.984176343950136	26.740940663546464	14.197421096101856
14-15	12.87759458764166	34.64419340425244	37.429265188408024	15.048946819697878
16-17	17.450161802073413	24.42069441748997	37.02947641119842	21.099667369238198
18-19	22.83458276728935	28.014189838739522	31.827032003863394	17.324195390107732
20-21	26.876475287384853	27.854238221395782	29.049407177061564	16.219879314157794
22-23	19.03731151299607	26.623910600405292	30.962959285453156	23.37581860114548
24-25	18.055396336096983	26.23513429346428	37.845211606421536	17.864257764017204
26-27	15.869285588557863	37.5188092089526	30.407551666087457	16.20435353640208
28-29	14.129405552270871	29.24004928531776	39.55115157040534	17.079393592006028
30-31	22.22054728366589	29.337762392414035	31.791286608565354	16.650403715354724
32-33	26.33460759048053	30.30816863522095	27.65736774880736	15.69985602549116
34-35	17.616793114498098	30.768751664282135	31.1874511998628	20.42700402135697
36-37	22.33581715688708	31.059950263351492	30.78314911516607	15.82108346459536
38-39	18.27523954379488	33.19217211949433	29.19658613421674	19.336002202494054
40-41	19.407402726940383	28.05318481542829	29.942049131865307	22.597363325766022
42-43	21.496883561180137	26.661912649446894	27.596573496955774	24.24463029241719
44-45	17.204683007848644	35.2090789693411	29.04349474425343	18.542743278556824
46-47	20.024913457328935	32.602824427825446	27.136667464017656	20.235594650827966
48-49	20.31146334968655	26.958617483650542	32.56202412814183	20.16789503852108
50-51	17.12461693302703	33.977938953002926	28.836785261343067	20.06065885262697
52-53	22.250154580781434	26.35446614342389	31.11108603718063	20.284293238614055
54-55	21.484878163264383	27.93010692025437	31.464252348048223	19.120762568433026
56-57	22.5165299886716	25.491296086511078	29.962223616332757	22.029950308484565
58-59	23.74090004377908	27.22991239670168	28.99285543424788	20.036332125271365
60-61	24.507756118916628	30.253783280001084	26.526423158683375	18.712037442398913
62-63	22.95905978778428	26.49631939774425	31.782936989714173	18.7616838247573
64-65	18.107118839899446	33.190547328798964	30.94305559943493	17.75927823186666
66-67	17.038367626948055	30.722129197940127	25.73821913913173	26.50128403598009
68-69	16.204037604877982	34.840386880717794	24.384949022191932	24.570626492212288
70-71	18.500092522803484	31.780590069820867	29.07969147030018	20.63962593707547
72-73	22.548755004129674	32.238645646689264	26.189188823245342	19.02341052593572
74-75	21.287511226852374	34.10480802646603	23.89724101513312	20.710439731548473
76-77	26.7143572824473	29.739356492618484	24.924605198427564	18.621681026506657
78-79	18.393352800732963	31.15955895959236	30.67379167475301	19.773296564921672
80-81	15.015277545843922	31.191783975050434	30.696538744488123	23.09639973461752
82-83	17.909751903487432	27.983093150153227	32.48218371869457	21.62497122766477
84-85	15.31699215135828	27.778820853285914	30.462207819756554	26.441979175599258
86	17.670050142846183	26.69973416618901	32.942631348531144	22.687584342433663
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1013.0
1	1132.0
2	4200.5
3	4553.0
4	2057.0
5	2576.0
6	3392.5
7	3791.0
8	4084.5
9	4206.0
10	3914.5
11	3621.5
12	3362.5
13	3348.5
14	3420.0
15	3366.5
16	3366.5
17	3636.5
18	4149.0
19	3985.5
20	3663.0
21	3800.0
22	4115.0
23	4837.0
24	5239.0
25	5308.5
26	7129.0
27	9584.5
28	13584.0
29	16874.0
30	17213.5
31	21087.5
32	22986.5
33	22704.0
34	24594.5
35	26273.0
36	27414.0
37	31163.0
38	34926.0
39	36495.0
40	41922.0
41	51068.0
42	58848.5
43	61355.0
44	66990.5
45	70694.0
46	70406.5
47	72891.0
48	65508.5
49	51197.5
50	45109.0
51	40780.0
52	32096.5
53	24498.5
54	20312.0
55	19128.0
56	17467.5
57	16048.0
58	14216.0
59	10088.5
60	6840.0
61	5750.5
62	5460.0
63	4848.0
64	4390.0
65	3237.5
66	1516.0
67	750.0
68	369.5
69	177.0
70	127.0
71	86.0
72	65.0
73	62.5
74	57.5
75	30.5
76	24.5
77	14.5
78	8.0
79	9.0
80	8.5
81	4.5
82	2.0
83	2.0
84	0.5
85	0.0
86	0.0
87	0.5
88	1.0
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1107835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.151343489912684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93885173180504	14.509975559731947
2	8.458647473853858	3.070716315160072
3	2.6891676521722276	1.464360172696204
4	1.2893513933027831	0.9361384007614527
5	0.8015128231143014	0.7274267281958654
6	0.5337494304537672	0.5812961549826954
7	0.400167594385154	0.5084505621451889
8	0.3174518265196436	0.46097417157265785
9	0.23254331543462523	0.37988762352632965
>10	2.6289829968807474	11.602600501740685
>50	1.106720209855043	14.83818670663777
>100	1.5952905439549976	48.871238875387654
>500	0.0060504066142414575	0.7470710167411787
>1k	0.0010084011023735761	0.25076253655161457
>5k	0.0	0.0
>10k+	5.042005511867881E-4	1.0509146741686826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11483	1.0365261974933089	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1496	0.13503816001480365	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1244	0.11229109027968967	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8053229948503162E-4	0.0	0.0	0.057950868134695147	0.0
2	1.8053229948503162E-4	0.0	0.0	0.17457473360202558	0.0
3	1.8053229948503162E-4	0.0	0.0	0.24516286270067295	0.0
4	1.8053229948503162E-4	0.0	0.0	0.37189653693916513	0.0
5	1.8053229948503162E-4	0.0	0.0	0.6370984848826766	0.0
6	1.8053229948503162E-4	0.0	0.0	0.8613196008430858	0.0
7	1.8053229948503162E-4	0.0	0.0	1.0194658951919735	0.0
8	1.8053229948503162E-4	0.0	0.0	1.3691569592944797	0.0
9	1.8053229948503162E-4	0.0	0.0	1.4734143622470854	0.0
10	1.8053229948503162E-4	0.0	0.0	1.6605360906633209	0.0
11	1.8053229948503162E-4	0.0	0.0	2.0327034260517136	0.0
12	1.8053229948503162E-4	0.0	0.0	2.305848795172566	0.0
13	1.8053229948503162E-4	0.0	0.0	2.4032459707447407	0.0
14	1.8053229948503162E-4	0.0	0.0	2.4405258905884	0.0
15	1.8053229948503162E-4	0.0	0.0	2.4972130326267	0.0
16	1.8053229948503162E-4	0.0	0.0	2.6220511177205994	0.0
17	1.8053229948503162E-4	0.0	0.0	2.7815514043156244	0.0
18	1.8053229948503162E-4	0.0	0.0	2.9782413446045664	0.0
19	1.8053229948503162E-4	0.0	0.0	3.1178830782562383	0.0
20	1.8053229948503162E-4	0.0	0.0	3.2498521891797965	0.0
21	1.8053229948503162E-4	0.0	0.0	3.417566695401391	0.0
22	1.8053229948503162E-4	0.0	0.0	3.609021199005267	0.0
23	1.8053229948503162E-4	0.0	0.0	3.8173554726109935	0.0
24	1.8053229948503162E-4	0.0	0.0	3.964128232092324	0.0
25	1.8053229948503162E-4	0.0	0.0	4.082828219003733	0.0
26	1.8053229948503162E-4	0.0	0.0	4.206853908749949	0.0
27	1.8053229948503162E-4	0.0	0.0	4.331240663095136	0.0
28	1.8053229948503162E-4	0.0	0.0	4.459960192627964	0.0
29	1.8053229948503162E-4	0.0	0.0	4.609531202751312	0.0
30	1.8053229948503162E-4	0.0	0.0	4.797555592664973	0.0
31	1.8053229948503162E-4	0.0	0.0	4.981066675091507	0.0
32	1.8053229948503162E-4	0.0	0.0	5.142372284681383	0.0
33	1.8053229948503162E-4	0.0	0.0	5.276959113947474	0.0
34	1.8053229948503162E-4	0.0	0.0	5.441243506478853	0.0
35	1.8053229948503162E-4	0.0	0.0	5.637933446767795	0.0
36	1.8053229948503162E-4	0.0	0.0	5.8086267359308925	0.0
37	1.8053229948503162E-4	0.0	0.0	5.994845802849702	0.0
38	1.8053229948503162E-4	0.0	0.0	6.161747913723614	0.0
39	1.8053229948503162E-4	0.0	0.0	6.3505846989849575	0.0
40	1.8053229948503162E-4	0.0	0.0	6.5436639932841985	0.0
41	2.707984492275474E-4	0.0	0.0	6.75253986378838	0.0
42	2.707984492275474E-4	0.0	0.0	6.9700812846678435	0.0
43	2.707984492275474E-4	0.0	0.0	7.145197615168324	0.0
44	2.707984492275474E-4	0.0	0.0	7.342699950804948	0.0
45	2.707984492275474E-4	0.0	0.0	7.528919017723759	0.0
46	2.707984492275474E-4	0.0	0.0	7.752688802935455	0.0
47	2.707984492275474E-4	0.0	0.0	7.994602084245398	0.0
48	2.707984492275474E-4	0.0	0.0	8.194270807475842	0.0
49	2.707984492275474E-4	0.0	0.0	8.44033633167394	0.0
50	2.707984492275474E-4	0.0	0.0	8.656343228007781	0.0
51	2.707984492275474E-4	0.0	0.0	8.9003326307618	0.0
52	2.707984492275474E-4	0.0	0.0	9.156598229880803	0.0
53	2.707984492275474E-4	0.0	0.0	9.384068927231944	0.0
54	2.707984492275474E-4	0.0	0.0	9.638980534104808	0.0
55	2.707984492275474E-4	0.0	0.0	9.898676246914025	0.0
56	2.707984492275474E-4	0.0	0.0	10.180848231009131	0.0
57	2.707984492275474E-4	0.0	0.0	10.448036034246977	0.0
58	2.707984492275474E-4	0.0	0.0	10.66666064892335	0.0
59	2.707984492275474E-4	0.0	0.0	10.924370506438233	0.0
60	2.707984492275474E-4	0.0	0.0	11.188669792884319	0.0
61	2.707984492275474E-4	0.0	0.0	11.46389128344925	0.0
62	2.707984492275474E-4	0.0	0.0	11.775128967761445	0.0
63	2.707984492275474E-4	0.0	0.0	12.09719859004274	0.0
64	2.707984492275474E-4	0.0	0.0	12.400041522428882	0.0
65	3.6106459897006324E-4	0.0	0.0	12.666507196468789	0.0
66	3.6106459897006324E-4	0.0	0.0	12.921418803341654	0.0
67	3.6106459897006324E-4	0.0	0.0	13.178948128557051	0.0
68	3.6106459897006324E-4	0.0	0.0	13.48928315137182	0.0
69	3.6106459897006324E-4	0.0	0.0	13.79961817418659	0.0
70	3.6106459897006324E-4	0.0	0.0	14.053807651861513	0.0
71	4.51330748712579E-4	0.0	0.0	14.317926406008114	0.0
72	4.51330748712579E-4	0.0	0.0	14.6015426484991	0.0
73	4.51330748712579E-4	0.0	0.0	14.906371436179576	0.0
74	4.51330748712579E-4	0.0	0.0	15.222393226428123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACACG	30	4.0599843E-8	80.0	1
CGTGCGT	30	4.0599843E-8	80.0	5
CGTTAAG	25	1.284774E-6	80.0	2
GTTCGCG	15	0.0013097785	80.0	1
GCGATAT	100	0.0	80.0	9
ACGTCGG	20	4.0908355E-5	80.0	2
CCTGGCG	15	0.0013097785	80.0	1
TACGTCG	15	0.0013097785	80.0	1
CTTACCG	15	0.0013097785	80.0	1
TCGCGAG	95	0.0	75.789474	1
GGAACGT	85	0.0	75.29411	8
CGTTTTT	5015	0.0	75.05483	1
TACGCGG	115	0.0	73.04348	2
ACTACGG	55	0.0	72.72727	2
TACGAGG	150	0.0	72.0	2
AATGCGG	230	0.0	71.30435	2
GCGTACG	85	0.0	70.588234	1
CGCGAGG	165	0.0	70.303024	2
GTACGCG	40	3.7143764E-9	70.0	1
GTAGTCG	75	0.0	69.333336	1
>>END_MODULE