FastQCFastQC Report
Sat 18 Jun 2016
SRR3549302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278442
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147261.1518707927305267No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA26780.20947371879209226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23200.1814708840917304No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC13100.1024684733448995TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTACG150.001309824880.000011
GGTACGT204.0910752E-580.000018
GGTCGAC150.001309824880.000019
CGGTAAT150.001309824880.0000180
GCTAACG204.0910752E-580.000011
CGCACGG1250.076.82
TCGATAG850.075.294121
CGTTTTT66200.074.924471
CTCGAGG650.073.846152
CGGGACC2900.073.1034556
TTTAGCG2300.073.043481
GTAGGGT8450.072.89944
AACGGGA5050.072.8712844
CGCATGG2800.072.857142
TAGCGGG10550.072.796213
GGACCGT1600.072.500018
TTAGCGG5750.072.3478242
GCGAATG503.6379788E-1272.01
AGTGCCG503.6379788E-1272.01
AGCGAAG2450.071.836731
Produced by FastQC (version 0.11.5)