##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1278442
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.771607159339258	31.0	31.0	33.0	30.0	34.0
2	32.15723826344879	33.0	31.0	34.0	30.0	34.0
3	32.255582967393124	34.0	31.0	34.0	30.0	34.0
4	35.88324304113914	37.0	35.0	37.0	35.0	37.0
5	35.82328959780733	37.0	35.0	37.0	35.0	37.0
6	35.86725639489316	37.0	35.0	37.0	35.0	37.0
7	36.19657285977776	37.0	35.0	37.0	35.0	37.0
8	36.179954976447895	37.0	35.0	37.0	35.0	37.0
9	37.97747179770377	39.0	39.0	39.0	35.0	39.0
10-11	37.40583812171377	39.0	37.0	39.0	34.5	39.0
12-13	36.19432637538504	38.0	35.0	39.0	32.5	39.0
14-15	37.142026388369594	39.0	35.0	41.0	32.5	41.0
16-17	37.41091852426625	39.0	35.0	41.0	33.0	41.0
18-19	37.30239267796271	38.5	35.0	41.0	33.0	41.0
20-21	37.00999184945426	38.0	35.0	41.0	33.0	41.0
22-23	36.74359884922429	38.0	35.0	41.0	32.0	41.0
24-25	36.69977636842344	38.0	35.0	41.0	32.0	41.0
26-27	36.63565261466691	38.0	35.0	41.0	32.0	41.0
28-29	36.63176155038711	38.0	35.0	41.0	32.0	41.0
30-31	36.4624128431325	38.0	35.0	41.0	31.5	41.0
32-33	36.01611649179235	37.0	35.0	40.5	30.5	41.0
34-35	35.66572320058321	37.0	35.0	40.0	30.0	41.0
36-37	35.44216124000933	37.0	35.0	40.0	29.0	41.0
38-39	35.43981111384012	37.0	35.0	40.0	29.0	41.0
40-41	35.343877547827745	37.0	35.0	40.0	29.0	41.0
42-43	35.23949072386546	37.0	35.0	40.0	28.5	41.0
44-45	34.99714613568703	36.0	34.0	40.0	27.0	41.0
46-47	34.90444032658502	36.0	34.0	40.0	27.0	41.0
48-49	34.85069326570935	36.0	34.0	40.0	27.0	41.0
50-51	34.65079995807397	35.5	34.0	40.0	26.5	41.0
52-53	34.43544251518645	35.5	34.0	40.0	26.0	41.0
54-55	34.29870811503376	35.0	34.0	39.5	25.5	41.0
56-57	34.00376943185533	35.0	33.5	39.0	24.5	41.0
58-59	33.62048806281396	35.0	33.0	39.0	23.5	40.0
60-61	33.23924120139983	35.0	33.0	38.0	22.5	40.0
62-63	32.72003031815288	35.0	32.0	37.5	21.5	40.0
64-65	32.42558715999631	35.0	32.0	37.0	20.5	40.0
66-67	32.07616301717246	35.0	32.0	36.5	19.5	39.5
68-69	31.737322850782434	35.0	31.5	36.0	18.0	39.0
70-71	31.454781288474564	35.0	31.0	35.5	17.0	39.0
72-73	31.18799249398878	35.0	31.0	35.0	15.0	38.0
74-75	30.894161408964976	34.5	31.0	35.0	13.0	37.0
76-77	28.930858810958963	32.5	28.0	34.5	10.0	35.5
78-79	29.805797603645686	33.5	29.5	35.0	10.0	36.0
80-81	30.198280406932813	34.0	30.0	35.0	10.0	36.0
82-83	30.050098870343746	34.0	30.0	35.0	10.0	36.0
84-85	29.831719389694644	34.0	30.0	35.0	10.0	35.0
86	29.470704185250483	34.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	2.0
12	17.0
13	59.0
14	256.0
15	814.0
16	2115.0
17	4397.0
18	8561.0
19	12540.0
20	14255.0
21	14344.0
22	12523.0
23	11285.0
24	12025.0
25	13808.0
26	16083.0
27	19742.0
28	24117.0
29	30138.0
30	37388.0
31	44870.0
32	53700.0
33	69086.0
34	184985.0
35	157057.0
36	93882.0
37	159455.0
38	200082.0
39	80766.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.75645355831551	25.010598838273463	30.030928270504255	15.202019332906772
2	30.1964422320293	26.255238798475016	29.721880226087695	13.826438743407993
3	27.742752506566585	25.196215393424183	34.01491815819568	13.04611394181355
4	26.344253395930362	27.230097259007447	32.74618637372677	13.679462971335422
5	23.793961712772266	30.94547895015965	32.26075175878139	12.999807578286696
6	20.39208661793026	40.398938708208895	28.391745577820505	10.817229096040338
7	85.88007903369883	3.922039482432523	8.306595058672979	1.891286425195668
8	86.60064359587686	3.153838813180418	8.073420616656838	2.1720969742858887
9	82.6680443852752	6.01810641390067	9.068381670814945	2.245467530009183
10-11	46.60950594551806	24.67429105113881	18.82435808585763	9.891844917485502
12-13	31.108646305424887	30.511474122408366	24.604557735118217	13.775321837048532
14-15	12.859754294680556	35.65265377701922	37.87598498797755	13.611606940322673
16-17	15.414152538793314	24.24810824425355	37.763504327924146	22.57423488902899
18-19	22.832205137190424	28.264559518538974	31.881109976048972	17.022125368221634
20-21	24.92576902198144	28.01722721875533	30.386439118864995	16.670564640398236
22-23	19.781890770171817	27.175460443258277	29.986538302089578	23.056110484480328
24-25	16.355258979288852	27.530032649115093	38.42892364299671	17.68578472859934
26-27	14.010921105533141	40.27233930049231	29.54001824095266	16.17672135302188
28-29	13.64762734641071	29.264409335738346	40.22446853279226	16.863494785058688
30-31	22.59492413421962	31.44546252391583	29.822041203277116	16.137572138587437
32-33	27.29607600501235	30.19808485641116	26.917059983949216	15.588779154627272
34-35	18.37596073971287	29.817856422113792	30.59923719652515	21.206945641648193
36-37	22.023173519017682	31.89558071465111	30.180015988210652	15.901229778120557
38-39	17.932686817235354	33.67704596688782	28.676897348491366	19.71336986738546
40-41	18.836716878825946	28.032597489757066	28.88042633142528	24.250259299991708
42-43	21.2850094098911	25.480350301382465	27.878699229218064	25.355941059508368
44-45	16.92133862936293	35.873547646275696	27.892231325316285	19.312882399045087
46-47	19.250580002847215	32.456067619805985	27.78866776905014	20.50468460829666
48-49	19.974703584519283	26.477071310235427	33.436127724214316	20.112097381030974
50-51	17.367936910708504	33.00231844698469	28.9065518811178	20.72319276118901
52-53	22.85418501582395	26.153161426173423	30.88493650865663	20.107717049346
54-55	21.772008429009684	28.18145054683748	31.58872283607704	18.457818188075798
56-57	23.747616239141077	24.18944308775838	29.523044455673393	22.53989621742715
58-59	25.11690010184271	27.57727765514587	28.021920431274943	19.283901811736474
60-61	25.287146385991697	30.849111653090244	26.072281730418744	17.79146023049931
62-63	22.76994185109688	26.36713280696348	32.361890488579064	18.501034853360576
64-65	17.733616386195074	33.17671040219267	30.918258317545888	18.17141489406637
66-67	16.383613804928185	29.997645571719328	25.639958637153658	27.978781986198825
68-69	15.221339724445848	35.841281810203355	23.693996286104493	25.2433821792463
70-71	18.13363453328348	32.58450520242608	28.724416125252457	20.557444139037987
72-73	22.980432432601557	32.69303574194215	25.90367807065162	18.422853754804677
74-75	21.45521658393576	35.154782148896864	22.613462323672096	20.776538943495286
76-77	27.751630500249526	29.507322193732684	24.886854468172977	17.854192837844813
78-79	18.100195394081233	31.23325109782063	31.194140993490514	19.472412514607623
80-81	13.825069889756438	30.43231527124422	32.34757619039424	23.395038648605098
82-83	17.126549346783037	26.73281228245005	33.30632128794267	22.834317082824253
84-85	14.371868258395768	27.664414967593366	30.499389100170365	27.464327673840504
86	17.22002249613201	27.187936566539584	32.83402766805221	22.758013269276198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1835.0
1	1688.0
2	5141.5
3	5493.0
4	2387.0
5	3021.0
6	4067.5
7	4623.0
8	4742.5
9	4638.5
10	4165.5
11	3829.5
12	3666.0
13	3539.0
14	3489.0
15	3566.5
16	3891.0
17	4130.0
18	4081.0
19	4114.0
20	4571.5
21	4955.0
22	4492.0
23	4354.0
24	5010.0
25	6349.5
26	7441.5
27	9422.0
28	13149.5
29	14980.0
30	19005.5
31	24226.0
32	25169.0
33	26790.0
34	27513.5
35	29679.5
36	32995.0
37	35843.0
38	38914.0
39	42812.5
40	50272.5
41	61429.0
42	74619.5
43	80438.0
44	82488.0
45	82737.0
46	83687.5
47	88756.0
48	85636.0
49	65931.5
50	51664.0
51	44143.5
52	31706.5
53	24914.0
54	20953.5
55	18874.0
56	18691.5
57	18504.0
58	15499.0
59	10604.5
60	7067.5
61	5118.5
62	4982.5
63	3959.0
64	2770.0
65	2000.0
66	893.0
67	555.0
68	465.0
69	286.0
70	157.0
71	118.0
72	81.5
73	38.0
74	30.0
75	19.0
76	6.0
77	3.0
78	2.5
79	2.0
80	3.5
81	4.5
82	2.0
83	0.0
84	0.0
85	1.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1278442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.295523098304717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52644209921395	14.36682335253857
2	9.684727790626786	3.543743219884119
3	3.2845491422316715	1.8027763409764948
4	1.5828668763957747	1.1583750999456133
5	0.9185251838812976	0.8402449359037433
6	0.6292281434421634	0.6907234819469696
7	0.45567855539381835	0.5835814274926809
8	0.32709939190772785	0.4787563584071409
9	0.27863194823930837	0.4587945520444119
>10	2.0981858698596496	8.50788759949003
>50	0.7328273788944961	9.779764203510545
>100	1.4691176160099313	54.789471429121775
>500	0.009522860209846343	1.159868060457239
>1k	0.0021642864113287142	0.6729863974986765
>5k	0.0	0.0
>10k+	4.328572822657428E-4	1.1662035407820086
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14726	1.1518707927305267	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2678	0.20947371879209226	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2320	0.1814708840917304	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1310	0.1024684733448995	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1101340537936019	0.0
2	0.0	0.0	0.0	0.388676216832676	0.0
3	0.0	0.0	0.0	0.5495751860467663	0.0
4	0.0	0.0	0.0	0.8356264891172224	0.0
5	0.0	0.0	0.0	1.3956831831244594	0.0
6	0.0	0.0	0.0	1.8572606344284683	0.0
7	0.0	0.0	0.0	2.1443288002115075	0.0
8	0.0	0.0	0.0	2.752412702336125	0.0
9	0.0	0.0	0.0	2.918708865947771	0.0
10	0.0	0.0	0.0	3.210939565502385	0.0
11	0.0	0.0	0.0	3.789534448962096	0.0
12	0.0	0.0	0.0	4.169841103468127	0.0
13	0.0	0.0	0.0	4.322526950772894	0.0
14	0.0	0.0	0.0	4.378454399964958	0.0
15	0.0	0.0	0.0	4.470441365349386	0.0
16	0.0	0.0	0.0	4.682105249983965	0.0
17	0.0	0.0	0.0	4.949305482767306	0.0
18	0.0	0.0	0.0	5.259448610105112	0.0
19	0.0	0.0	0.0	5.44334432066531	0.0
20	0.0	0.0	0.0	5.632637225623063	0.0
21	0.0	0.0	0.0	5.875980294765034	0.0
22	0.0	0.0	0.0	6.131603936666662	0.0
23	0.0	0.0	0.0	6.400681454457848	0.0
24	0.0	0.0	0.0	6.570497527459204	0.0
25	0.0	0.0	0.0	6.725764641649758	0.0
26	0.0	0.0	0.0	6.882283279178876	0.0
27	0.0	0.0	0.0	7.030041253338047	0.0
28	0.0	0.0	0.0	7.193130388394624	0.0
29	0.0	0.0	0.0	7.389384891923138	0.0
30	0.0	0.0	0.0	7.624671279573105	0.0
31	0.0	0.0	0.0	7.85565555574676	0.0
32	0.0	0.0	0.0	8.049172351972166	0.0
33	0.0	0.0	0.0	8.245974396961302	0.0
34	0.0	0.0	0.0	8.438708991100105	0.0
35	0.0	0.0	0.0	8.692611788411206	0.0
36	0.0	0.0	0.0	8.905370755967029	0.0
37	0.0	0.0	0.0	9.136980793809965	0.0
38	0.0	0.0	0.0	9.338163170484073	0.0
39	7.822020866022863E-5	0.0	0.0	9.562498728921609	0.0
40	7.822020866022863E-5	0.0	0.0	9.790745297792157	0.0
41	7.822020866022863E-5	0.0	0.0	10.05207901492598	0.0
42	7.822020866022863E-5	0.0	0.0	10.304417408063879	0.0
43	7.822020866022863E-5	0.0	0.0	10.53454126194227	0.0
44	7.822020866022863E-5	0.0	0.0	10.76740282312377	0.0
45	7.822020866022863E-5	0.0	0.0	10.99995150347063	0.0
46	7.822020866022863E-5	0.0	0.0	11.248222445758197	0.0
47	7.822020866022863E-5	0.0	0.0	11.520428771895793	0.0
48	7.822020866022863E-5	0.0	0.0	11.771280981069145	0.0
49	7.822020866022863E-5	0.0	0.0	12.064528543336342	0.0
50	7.822020866022863E-5	0.0	0.0	12.319213542734047	0.0
51	7.822020866022863E-5	0.0	0.0	12.595643760139295	0.0
52	7.822020866022863E-5	0.0	0.0	12.880991081331809	0.0
53	7.822020866022863E-5	0.0	0.0	13.164461117516478	0.0
54	7.822020866022863E-5	0.0	0.0	13.47663797027945	0.0
55	7.822020866022863E-5	0.0	0.0	13.776064929030804	0.0
56	7.822020866022863E-5	0.0	0.0	14.08292280760488	0.0
57	7.822020866022863E-5	0.0	0.0	14.38344484927748	0.0
58	7.822020866022863E-5	0.0	0.0	14.650801522478142	0.0
59	7.822020866022863E-5	0.0	0.0	14.94475306662328	0.0
60	7.822020866022863E-5	0.0	0.0	15.23549758221335	0.0
61	7.822020866022863E-5	0.0	0.0	15.54000885452762	0.0
62	7.822020866022863E-5	0.0	0.0	15.893798858297835	0.0
63	7.822020866022863E-5	0.0	0.0	16.29952708061844	0.0
64	7.822020866022863E-5	0.0	0.0	16.63407491305824	0.0
65	7.822020866022863E-5	0.0	0.0	16.929747301793903	0.0
66	7.822020866022863E-5	0.0	0.0	17.20578641815585	0.0
67	7.822020866022863E-5	0.0	0.0	17.516633527371596	0.0
68	1.5644041732045726E-4	0.0	0.0	17.853919067114504	0.0
69	1.5644041732045726E-4	0.0	0.0	18.177124969298568	0.0
70	1.5644041732045726E-4	0.0	0.0	18.45824839922343	0.0
71	1.5644041732045726E-4	0.0	0.0	18.792483350828586	0.0
72	1.5644041732045726E-4	0.0	0.0	19.093474713753146	0.0
73	1.5644041732045726E-4	0.0	0.0	19.42669280264572	0.0
74	2.3466062598068586E-4	0.0	0.0	19.761318855294178	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTACG	15	0.0013098248	80.00001	1
GGTACGT	20	4.0910752E-5	80.00001	8
GGTCGAC	15	0.0013098248	80.00001	9
CGGTAAT	15	0.0013098248	80.00001	80
GCTAACG	20	4.0910752E-5	80.00001	1
CGCACGG	125	0.0	76.8	2
TCGATAG	85	0.0	75.29412	1
CGTTTTT	6620	0.0	74.92447	1
CTCGAGG	65	0.0	73.84615	2
CGGGACC	290	0.0	73.103455	6
TTTAGCG	230	0.0	73.04348	1
GTAGGGT	845	0.0	72.8994	4
AACGGGA	505	0.0	72.871284	4
CGCATGG	280	0.0	72.85714	2
TAGCGGG	1055	0.0	72.79621	3
GGACCGT	160	0.0	72.50001	8
TTAGCGG	575	0.0	72.347824	2
GCGAATG	50	3.6379788E-12	72.0	1
AGTGCCG	50	3.6379788E-12	72.0	1
AGCGAAG	245	0.0	71.83673	1
>>END_MODULE