##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549301_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1253708
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8221427956111	31.0	31.0	33.0	30.0	34.0
2	32.236465748005116	33.0	31.0	34.0	30.0	34.0
3	32.337868147925995	34.0	31.0	34.0	30.0	34.0
4	35.961719953928664	37.0	35.0	37.0	35.0	37.0
5	35.90291200183775	37.0	35.0	37.0	35.0	37.0
6	35.95893700925574	37.0	35.0	37.0	35.0	37.0
7	36.174403449607084	37.0	35.0	37.0	35.0	37.0
8	36.14079434764714	37.0	35.0	37.0	35.0	37.0
9	37.90077434298896	39.0	38.0	39.0	35.0	39.0
10-11	37.481847447731056	39.0	37.0	39.0	35.0	39.0
12-13	36.301458952164296	38.0	35.0	39.0	33.0	39.0
14-15	37.28374988434308	39.0	35.0	41.0	33.0	41.0
16-17	37.55997568811877	39.0	35.0	41.0	33.5	41.0
18-19	37.46271021641403	38.5	35.0	41.0	33.5	41.0
20-21	37.123268735622645	38.0	35.0	41.0	33.0	41.0
22-23	36.88424218398542	38.0	35.0	41.0	32.5	41.0
24-25	36.84439957310634	38.0	35.0	41.0	33.0	41.0
26-27	36.77661943610474	38.0	35.0	41.0	33.0	41.0
28-29	36.79963994805808	38.0	35.0	41.0	33.0	41.0
30-31	36.6397518401414	38.0	35.0	41.0	32.5	41.0
32-33	36.22026261298484	38.0	35.0	41.0	31.5	41.0
34-35	35.886515041780065	38.0	35.0	41.0	30.0	41.0
36-37	35.62468413697607	37.0	35.0	40.0	30.0	41.0
38-39	35.644293567561185	37.0	35.0	40.5	30.0	41.0
40-41	35.53884676495643	37.0	35.0	40.0	29.0	41.0
42-43	35.42621048920482	37.0	35.0	40.0	29.0	41.0
44-45	35.24013805447521	37.0	35.0	40.0	29.0	41.0
46-47	35.14191901144446	36.0	35.0	40.0	28.0	41.0
48-49	35.073388699761026	36.0	35.0	40.0	28.0	41.0
50-51	34.929536223745885	36.0	34.0	40.0	27.5	41.0
52-53	34.73997773006155	36.0	34.0	40.0	27.0	41.0
54-55	34.61320419108756	35.5	34.0	40.0	27.0	41.0
56-57	34.33484710953428	35.0	34.0	39.5	25.5	41.0
58-59	34.04985251749211	35.0	34.0	39.0	25.5	41.0
60-61	33.685218168824	35.0	33.0	39.0	24.0	40.0
62-63	33.261595602803844	35.0	32.5	38.0	23.0	40.0
64-65	33.03396883484831	35.0	32.5	38.0	23.0	40.0
66-67	32.73627710758805	35.0	33.0	37.0	22.0	40.0
68-69	32.44265690256424	35.0	32.5	36.5	21.0	39.0
70-71	32.247713183612134	35.0	32.5	36.0	20.5	39.0
72-73	32.04554489562163	35.0	32.0	36.0	20.0	38.5
74-75	31.730820095269394	35.0	32.0	35.0	20.0	37.5
76-77	29.728558404349336	32.5	28.5	34.5	16.5	35.5
78-79	30.561214014746653	34.0	30.0	35.0	17.5	36.0
80-81	31.060315081342704	34.0	31.0	35.0	18.0	36.0
82-83	30.957506054041293	34.0	31.0	35.0	18.0	36.0
84-85	30.74239974539526	34.0	31.0	35.0	17.5	35.5
86	30.316515488455046	34.0	30.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	8.0
11	12.0
12	18.0
13	63.0
14	211.0
15	678.0
16	1740.0
17	3753.0
18	7375.0
19	11576.0
20	13897.0
21	13754.0
22	11679.0
23	10131.0
24	10062.0
25	10940.0
26	12722.0
27	15426.0
28	18832.0
29	23667.0
30	29834.0
31	37164.0
32	47555.0
33	66439.0
34	187056.0
35	156522.0
36	92069.0
37	158064.0
38	220520.0
39	91835.0
40	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.16171548717883	24.943846573524297	27.968235027614085	14.926202911682784
2	31.120324668902168	26.916235678483346	28.674380318224017	13.289059334390462
3	28.777833434898714	23.877250524045472	32.68847291394806	14.65644312710775
4	28.173226939606348	26.718183181410666	30.301393945001546	14.807195933981438
5	24.730639032374366	30.62228206248983	30.245639335475243	14.40143956966056
6	21.310863454648132	40.33794153024468	26.944471918500955	11.406723096606228
7	83.12765013862877	5.288552039230825	9.159230059950165	2.4245677621902386
8	83.10188656369745	6.143136998407923	8.449734706965257	2.305241730929371
9	78.38396181567	8.202468198336454	9.950722177731976	3.4628478082615732
10-11	45.45053553139965	24.16631304897153	20.68356427493483	9.69958714469398
12-13	32.350036850686124	31.432239405028923	22.28964001186879	13.92808373241616
14-15	12.5428329403657	36.44875840307312	35.09940113646878	15.909007520092397
16-17	13.954006834127245	24.97878293829185	36.658815290322785	24.40839493725812
18-19	22.572760164248773	28.19472317317908	30.16029250830337	19.072224154268778
20-21	24.92853200266729	29.015568218436826	28.819469924416214	17.23642985447967
22-23	20.077641683709444	27.853535272966273	27.778358278004127	24.290464765320156
24-25	15.69392553928028	28.12317541245649	38.121596097336855	18.061302950926372
26-27	14.453325654777668	40.16417698539054	28.288006457644045	17.09449090218775
28-29	13.032261100670969	29.408043978342647	39.91507591879449	17.644619002191895
30-31	21.7556639983154	34.68542914299023	27.41284254387784	16.146064314816527
32-33	26.244747580776384	31.71827092113953	24.71955192118101	17.317429576903073
34-35	17.80797442466667	31.449548060632935	28.518562536092933	22.22391497860746
36-37	22.01453608017178	31.997203495550796	29.91342481662397	16.074835607653455
38-39	17.087033025233946	34.2247556847368	29.484377542458052	19.203833747571206
40-41	18.801826262574696	28.44948744045663	29.746081224655185	23.00260507231349
42-43	19.657049328870837	26.72592023022905	27.15524667625954	26.461783764640572
44-45	16.05026848357034	38.200841025182896	25.638306527516775	20.11058396372999
46-47	19.764171561480026	34.49599109202462	26.016943339278363	19.722894007216993
48-49	19.04957135154278	28.03104869714479	33.26005736583	19.659322585482425
50-51	16.657706579203452	34.89257466650927	28.128519559578468	20.321199194708818
52-53	21.062799312120525	28.03766905850485	29.933764481043433	20.965767148331192
54-55	21.447538023207958	29.608409613721854	29.859464883369974	19.084587479700218
56-57	22.74732234300172	25.151151623823093	27.823743646845994	24.277782386329193
58-59	23.775592083643083	27.845718460758007	28.17629783011674	20.202391625482168
60-61	24.013765565825533	33.65532484438163	24.602858081786188	17.728051508006647
62-63	23.379447207802773	26.209212990584728	32.45735849177001	17.953981309842483
64-65	16.28230018473201	34.73440386437671	30.181788741876098	18.801507209015178
66-67	15.528576032058503	29.281738650467254	23.612715241507594	31.576970075966653
68-69	15.207488506095517	35.568130697100116	22.831791772884912	26.392589023919445
70-71	16.962602137020742	32.00693462911619	29.288000076572857	21.742463157290214
72-73	21.938641214700713	33.36813675911775	25.798192242531755	18.89502978364978
74-75	21.128404700297036	34.555933279519635	22.57543223780976	21.740229782373564
76-77	28.225591605062743	28.41291592619653	25.41855838839666	17.942934080344067
78-79	16.60797410561311	31.55076780239099	30.67077820353703	21.170479888458875
80-81	13.023965708123422	29.742292463635874	32.9010822296739	24.33265959856681
82-83	16.131826549722902	26.733976332606957	32.17742887498524	24.9567682426849
84-85	13.698684223120535	28.20999786234115	28.52829366965833	29.563024244879987
86	15.10335740060684	27.471947215779114	32.964534006323646	24.460161377290408
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1543.0
1	1525.5
2	4173.0
3	4593.0
4	2428.5
5	2878.5
6	3492.0
7	3736.0
8	4006.0
9	4053.5
10	3594.0
11	3234.5
12	3140.5
13	3251.5
14	3334.0
15	3328.5
16	3424.0
17	3543.5
18	3813.0
19	4006.5
20	4171.0
21	4393.0
22	4700.5
23	5347.5
24	5943.5
25	5391.0
26	5597.0
27	10289.0
28	18452.5
29	22939.0
30	27380.5
31	31524.0
32	27936.0
33	23758.0
34	23664.5
35	28935.5
36	33412.0
37	34198.5
38	39087.0
39	41167.0
40	41818.5
41	52193.0
42	67465.0
43	75036.0
44	78865.0
45	80006.5
46	83462.0
47	89469.0
48	81762.0
49	64096.0
50	54001.0
51	43340.0
52	29224.0
53	23656.0
54	19582.5
55	17750.5
56	17943.0
57	18007.0
58	15930.0
59	11539.5
60	8139.0
61	7202.0
62	6546.0
63	5575.5
64	5411.0
65	4330.0
66	2622.5
67	1415.0
68	823.5
69	703.0
70	448.0
71	303.0
72	318.0
73	310.5
74	195.5
75	69.0
76	25.5
77	21.5
78	22.5
79	18.0
80	15.5
81	8.5
82	2.5
83	2.5
84	2.5
85	2.0
86	3.0
87	1.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1253708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.18890961500398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72110199156405	13.067866241649481
2	8.83737962639199	2.861350800102751
3	2.734825616024067	1.328215341318336
4	1.2257196370475694	0.7937225766999434
5	0.7444228502821976	0.6025697119281068
6	0.49676095647051943	0.4825210934738499
7	0.3756910037808013	0.4257419392364258
8	0.2974788581931577	0.3852686678130894
9	0.20989651029006184	0.3058196070231508
>10	1.9225853267649202	7.0518962480211
>50	0.7058949114695635	8.35417632113008
>100	1.7002468795381804	58.918940517310745
>500	0.020496948562500856	2.2360840267427915
>1k	0.006998958045731999	2.2270195868066662
>5k	0.0	0.0
>10k+	4.999255746951428E-4	0.9588073207434886
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11847	0.9449568799114307	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	4001	0.31913332291091706	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3579	0.2854731723814477	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA	2978	0.23753537506341188	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTG	2689	0.21448375538801698	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2354	0.18776301977813015	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1918	0.15298618179033716	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1799	0.14349433839458628	No Hit
TATGAAGGGATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCA	1485	0.11844863397218491	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTG	1255	0.1001030542997253	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.392901696407776E-4	0.0	0.0	0.05168667664240796	0.0
2	2.392901696407776E-4	0.0	0.0	0.16000536009979996	0.0
3	2.392901696407776E-4	0.0	0.0	0.22987808963490702	0.0
4	2.392901696407776E-4	0.0	0.0	0.36858662463667774	0.0
5	2.392901696407776E-4	0.0	0.0	0.619761539369614	0.0
6	2.392901696407776E-4	0.0	0.0	0.8529896913794919	0.0
7	2.392901696407776E-4	0.0	0.0	0.9861945524795247	0.0
8	2.392901696407776E-4	0.0	0.0	1.258347238750969	0.0
9	2.392901696407776E-4	0.0	0.0	1.3410618740567979	0.0
10	2.392901696407776E-4	0.0	0.0	1.4953242700852192	0.0
11	2.392901696407776E-4	0.0	0.0	1.7804783889071458	0.0
12	2.392901696407776E-4	0.0	0.0	1.9756594039441402	0.0
13	2.392901696407776E-4	0.0	0.0	2.0594907267082925	0.0
14	2.392901696407776E-4	0.0	0.0	2.0893222345235096	0.0
15	2.392901696407776E-4	0.0	0.0	2.1444387369307685	0.0
16	2.392901696407776E-4	0.0	0.0	2.2541931614060053	0.0
17	2.392901696407776E-4	0.0	0.0	2.385563464538792	0.0
18	2.392901696407776E-4	0.0	0.0	2.5610429222753623	0.0
19	2.392901696407776E-4	0.0	0.0	2.6577161508102365	0.0
20	2.392901696407776E-4	0.0	0.0	2.7605311603658906	0.0
21	2.392901696407776E-4	0.0	0.0	2.9069767441860463	0.0
22	2.392901696407776E-4	0.0	0.0	3.0417768730836845	0.0
23	2.392901696407776E-4	0.0	0.0	3.1900570148710865	0.0
24	2.392901696407776E-4	0.0	0.0	3.2993328590070417	0.0
25	2.392901696407776E-4	0.0	0.0	3.4003133105954495	0.0
26	2.392901696407776E-4	0.0	0.0	3.5026497398118224	0.0
27	2.392901696407776E-4	0.0	0.0	3.592463316816994	0.0
28	2.392901696407776E-4	0.0	0.0	3.6857864829768974	0.0
29	2.392901696407776E-4	0.0	0.0	3.8064684918657297	0.0
30	2.392901696407776E-4	0.0	0.0	3.949324723141274	0.0
31	2.392901696407776E-4	0.0	0.0	4.0877142045835235	0.0
32	2.392901696407776E-4	0.0	0.0	4.2027330127908575	0.0
33	2.392901696407776E-4	0.0	0.0	4.323095968120168	0.0
34	2.392901696407776E-4	0.0	0.0	4.456460355999962	0.0
35	2.392901696407776E-4	0.0	0.0	4.62508016220683	0.0
36	2.392901696407776E-4	0.0	0.0	4.768494737211536	0.0
37	2.392901696407776E-4	0.0	0.0	4.911829548826361	0.0
38	2.392901696407776E-4	0.0	0.0	5.049341632979928	0.0
39	2.392901696407776E-4	0.0	0.0	5.2005730201929	0.0
40	2.392901696407776E-4	0.0	0.0	5.369352353179528	0.0
41	2.392901696407776E-4	0.0	0.0	5.525210017005555	0.0
42	2.392901696407776E-4	0.0	0.0	5.683540345917869	0.0
43	2.392901696407776E-4	0.0	0.0	5.823844148717245	0.0
44	2.392901696407776E-4	0.0	0.0	5.9824137677992	0.0
45	2.392901696407776E-4	0.0	0.0	6.138430958404988	0.0
46	2.392901696407776E-4	0.0	0.0	6.311836568004671	0.0
47	2.392901696407776E-4	0.0	0.0	6.496967395916752	0.0
48	2.392901696407776E-4	0.0	0.0	6.664550278055177	0.0
49	2.392901696407776E-4	0.0	0.0	6.853429985291631	0.0
50	2.392901696407776E-4	0.0	0.0	7.054114674230363	0.0
51	2.392901696407776E-4	0.0	0.0	7.223691641115794	0.0
52	2.392901696407776E-4	0.0	0.0	7.414405906319494	0.0
53	2.392901696407776E-4	0.0	0.0	7.612139349832656	0.0
54	2.392901696407776E-4	0.0	0.0	7.815376467247557	0.0
55	2.392901696407776E-4	0.0	0.0	8.023638678224914	0.0
56	2.392901696407776E-4	0.0	0.0	8.238760540731972	0.0
57	2.392901696407776E-4	0.0	0.0	8.438807122551662	0.0
58	2.392901696407776E-4	0.0	0.0	8.618514039951886	0.0
59	2.392901696407776E-4	0.0	0.0	8.818082041432294	0.0
60	2.392901696407776E-4	0.0	0.0	9.053623331748701	0.0
61	2.392901696407776E-4	0.0	0.0	9.274408394937257	0.0
62	2.392901696407776E-4	0.0	0.0	9.532682251369538	0.0
63	2.392901696407776E-4	0.0	0.0	9.817676843411704	0.0
64	3.190535595210368E-4	0.0	0.0	10.080098396117757	0.0
65	3.190535595210368E-4	0.0	0.0	10.307184767106854	0.0
66	3.190535595210368E-4	0.0	0.0	10.532356816738826	0.0
67	3.190535595210368E-4	0.0	0.0	10.753301406707143	0.0
68	3.190535595210368E-4	0.0	0.0	11.0229814278923	0.0
69	3.190535595210368E-4	0.0	0.0	11.316191649092133	0.0
70	3.190535595210368E-4	0.0	0.0	11.529798007191467	0.0
71	3.190535595210368E-4	0.0	0.0	11.752577155127032	0.0
72	3.190535595210368E-4	0.0	0.0	11.971926477297744	0.0
73	3.190535595210368E-4	0.0	0.0	12.22278233847116	0.0
74	3.190535595210368E-4	0.0	0.0	12.516311613230513	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	15	0.0013098188	80.00001	6
GATACGA	20	4.0910443E-5	80.00001	80
CGACCGG	15	0.0013098188	80.00001	2
ATATGCG	40	4.1836756E-11	80.00001	1
ACGGGTA	55	0.0	80.00001	5
GTATGCG	30	4.06053E-8	80.00001	1
GGCACGT	50	0.0	80.0	8
CTAACGG	70	0.0	80.0	2
GTGCCGG	35	1.2896635E-9	80.0	2
CGGGATA	260	0.0	75.38461	6
CGTTTTT	5710	0.0	75.23643	1
ATTAGCG	70	0.0	74.28571	1
GTAGGGT	485	0.0	73.40206	4
TAGCGGG	815	0.0	73.12883	3
CGTAAGG	215	0.0	72.558136	2
TTGCGGA	50	3.6379788E-12	72.0	3
CGGGATC	240	0.0	71.66667	6
TAGCACG	45	1.1823431E-10	71.111115	1
TCCGCTA	405	0.0	71.11111	2
TATTGCG	175	0.0	70.85715	1
>>END_MODULE