FastQCFastQC Report
Sat 18 Jun 2016
SRR3549300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1357173
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112940.8321709907285217No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA33410.24617347972587136No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA24930.18369065697593454No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA18540.1366074921914892No Hit
GGAATGGGGATTCATTACATATCTTGAATATATACATAATTTTGTATTTT17070.12577615381384688No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17030.1254814235178566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG204.091167E-580.01
GCGAACG204.091167E-580.01
ACGTGCG550.080.01
GCGATAC304.060712E-880.09
TATAGCG204.091167E-580.01
TCGGTCG304.060712E-880.01
CGATTAC850.075.294129
CGTTTTT61950.074.382571
CGAACGG503.6379788E-1272.000012
TCTAGCG503.6379788E-1272.000011
AGGGCGA4150.071.32536
GTGTCGA451.1823431E-1071.111119
CGTAAGG2400.070.02
TTGCGAG1100.069.0909041
AGGGATT21300.068.920196
CGAAACG2450.068.5714380
CCGTAAG351.1816519E-768.571431
TGCGATG351.1816519E-768.571431
GCGTAAG700.068.571431
TACGCGG1700.068.23532
Produced by FastQC (version 0.11.5)