##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549300_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1357173 Sequences flagged as poor quality 0 Sequence length 86 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.737527198080127 31.0 31.0 33.0 30.0 34.0 2 32.13487963583125 33.0 31.0 34.0 30.0 34.0 3 32.242850395638584 34.0 31.0 34.0 30.0 34.0 4 35.8648890008864 37.0 35.0 37.0 35.0 37.0 5 35.807524169726335 37.0 35.0 37.0 35.0 37.0 6 35.85857440429481 37.0 35.0 37.0 35.0 37.0 7 36.15714356239035 37.0 35.0 37.0 35.0 37.0 8 36.14512888187431 37.0 35.0 37.0 35.0 37.0 9 37.91454000337466 39.0 38.0 39.0 35.0 39.0 10-11 37.382804181928165 39.0 37.0 39.0 34.5 39.0 12-13 36.4193282654459 39.0 35.0 39.0 33.0 39.0 14-15 37.43985107278144 40.0 35.0 41.0 33.0 41.0 16-17 37.6626399139977 39.5 35.0 41.0 33.0 41.0 18-19 37.54483400421317 39.0 35.5 41.0 33.0 41.0 20-21 37.28227794098468 39.0 35.0 41.0 33.0 41.0 22-23 36.99797999223385 38.5 35.0 41.0 32.5 41.0 24-25 36.957924671357304 38.0 35.0 41.0 32.5 41.0 26-27 36.88699487832429 38.0 35.0 41.0 32.5 41.0 28-29 36.87248788474277 38.0 35.0 41.0 32.0 41.0 30-31 36.733031824240534 38.0 35.0 41.0 32.0 41.0 32-33 36.33902494376177 38.0 35.0 41.0 31.0 41.0 34-35 36.024686241179275 38.0 35.0 41.0 30.0 41.0 36-37 35.78104044215439 38.0 35.0 41.0 30.0 41.0 38-39 35.74901652184357 38.0 35.0 40.5 30.0 41.0 40-41 35.65869310692152 38.0 35.0 40.0 29.0 41.0 42-43 35.55009715047382 38.0 35.0 40.0 29.0 41.0 44-45 35.362707259870334 38.0 35.0 40.0 28.0 41.0 46-47 35.26304863123566 37.0 35.0 40.0 27.5 41.0 48-49 35.1612123141265 37.0 34.5 40.0 27.0 41.0 50-51 34.96277887933226 36.5 34.0 40.0 27.0 41.0 52-53 34.73288998528559 36.0 34.0 40.0 26.0 41.0 54-55 34.58067873439863 36.0 34.0 40.0 26.0 41.0 56-57 34.296320734350004 35.5 34.0 39.5 25.5 41.0 58-59 33.981083104364735 35.0 34.0 39.0 25.0 41.0 60-61 33.57565137237478 35.0 33.0 39.0 24.0 40.0 62-63 33.11098253501949 35.0 32.5 38.0 22.0 40.0 64-65 32.785757232128844 35.0 32.0 37.5 22.0 40.0 66-67 32.438294159992864 35.0 32.0 37.0 20.0 40.0 68-69 32.030172645639134 35.0 32.0 36.5 18.5 39.0 70-71 31.723650927332034 35.0 31.5 36.0 18.0 39.0 72-73 31.44249001416916 35.0 31.0 35.5 17.5 38.5 74-75 31.147900083482355 34.5 31.0 35.0 16.0 37.5 76-77 29.163947411273284 32.5 28.0 34.5 11.0 35.5 78-79 30.0309231026553 34.0 29.5 35.0 11.0 36.0 80-81 30.331401744655984 34.0 30.0 35.0 10.5 36.0 82-83 30.13680385625119 34.0 30.0 35.0 10.0 36.0 84-85 29.895653317594736 34.0 30.0 35.0 10.0 35.5 86 29.508370708818994 34.0 29.0 35.0 10.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 7.0 10 0.0 11 12.0 12 28.0 13 75.0 14 289.0 15 795.0 16 2052.0 17 4319.0 18 8585.0 19 13011.0 20 14500.0 21 14486.0 22 12620.0 23 11657.0 24 12300.0 25 13380.0 26 15850.0 27 19412.0 28 23875.0 29 30110.0 30 37842.0 31 46198.0 32 55024.0 33 70311.0 34 173434.0 35 154217.0 36 108107.0 37 179152.0 38 240516.0 39 94818.0 40 190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.067078404890164 25.286091014189054 29.00271372920033 14.644116851720451 2 29.63144713312157 26.597051370753768 30.49154381939517 13.279957676729495 3 29.61265807675219 24.333154284678518 32.29735634292754 13.75683129564175 4 27.034946908021308 26.449612540184635 32.10298171272196 14.412458839072102 5 24.268239936986664 31.195065035923943 30.72054925937961 13.816145767709791 6 22.32552519096681 40.26818983283634 27.24177389323248 10.164511082964369 7 84.84371557642247 4.918164449189602 8.295921006386068 1.9421989680018685 8 85.57118362950044 3.943417677775788 8.136250868533342 2.3491478241904313 9 80.78564781350646 6.991960494351125 9.452884783295866 2.7695069088465507 10-11 42.40369503372083 26.62265606521792 20.263628881505895 10.710020019555355 12-13 29.511712950375525 29.500107944970903 26.450865143942593 14.537313960710978 14-15 14.579386710463588 35.31178412774201 35.65897641641854 14.449852745375866 16-17 17.197991707763123 26.259658864418906 35.32862059590045 21.21372883191752 18-19 23.05605107086569 29.0793436061578 30.808820983028696 17.05578433994782 20-21 25.38648352126074 28.90397908004359 29.487508224817322 16.22202917387835 22-23 20.192783086607236 27.286572898223 29.81930085552837 22.7013431596414 24-25 17.198175914198117 29.001240077720382 36.336156112743176 17.464427895338325 26-27 16.472844655766067 37.06966613688896 29.447130174266654 17.010359033078316 28-29 14.885648329284477 30.49419639205908 36.685300989630655 17.934854289025793 30-31 22.108198438960986 31.4596591591492 29.578027266973333 16.854115134916476 32-33 24.819127701479474 31.780583610195606 27.063167333862374 16.337121354462546 34-35 18.66051711904083 31.135492674846905 29.076985763789875 21.127004442322388 36-37 22.337903863398402 32.09052935771637 29.375215982045027 16.196350796840196 38-39 18.794140466985418 35.041000668300946 27.40435449275811 18.760504371955527 40-41 19.066950197211412 29.687077476489733 28.47636226184871 22.769610064450145 42-43 21.130062269143284 27.525599168271103 27.642496571918247 23.701841990667365 44-45 17.7498741870049 35.22649654833982 27.112866230023734 19.910763034631547 46-47 20.355400527419864 32.808529200035665 27.18466989838436 19.65140037416011 48-49 20.24203988732461 28.499351224935953 31.45306456877642 19.80554431896302 50-51 18.67937985798421 33.45214648390441 28.022661812458693 19.84581184565269 52-53 21.49847513913112 28.898047632836786 29.631889228565555 19.97158799946654 54-55 21.94513890270437 28.78008183186668 30.200350286956784 19.074428978472163 56-57 22.40738653067811 26.900734099484737 29.135416044969947 21.556463324867206 58-59 23.470405025741005 29.190383245172136 27.718500146996732 19.620711582090124 60-61 23.397717166492406 31.654328519650775 26.422607876814524 18.52534643704229 62-63 21.849719969377524 28.73465652499718 30.877345776846433 18.538277728778866 64-65 17.71712228286298 33.81786993994134 30.099589367015113 18.365418410180574 66-67 16.52608031547931 31.59361407867678 24.93215676999174 26.94814883585217 68-69 16.201803307316016 35.80799942232862 23.476483838095806 24.513713432259557 70-71 17.81887791755362 33.4675829831569 27.88155968325335 20.831979416036127 72-73 21.873040504047754 33.161100316614025 25.4632607633662 19.502598415972024 74-75 21.025027759909754 35.32029446503873 22.971316110768488 20.683361664283034 76-77 26.070920951124137 30.803515837700868 24.471309110923958 18.654254100251038 78-79 17.763910717351436 32.28589870267092 29.733129085238215 20.217061494739433 80-81 15.320375515870122 30.912455523356268 31.08627271541653 22.680896245357076 82-83 17.492832527614386 28.79249734558527 31.26568978310061 22.448980343699734 84-85 15.390742374037798 28.581323088508242 29.742928867579888 26.285005669874067 86 16.855994040553416 29.234445424422677 30.846251730619457 23.06330880440445 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1293.0 1 1287.5 2 3960.0 3 4482.0 4 2530.5 5 3286.5 6 4289.0 7 4740.0 8 5085.0 9 5093.0 10 4542.0 11 4049.0 12 3787.0 13 3796.0 14 3788.0 15 3957.5 16 4088.5 17 4282.5 18 4514.0 19 4443.0 20 4825.5 21 5278.0 22 5499.0 23 6193.0 24 6951.0 25 7292.0 26 8605.5 27 10536.5 28 16064.0 29 20918.0 30 23669.0 31 30823.0 32 32504.0 33 29387.0 34 30689.5 35 33410.5 36 34434.0 37 35501.5 38 40292.5 39 47726.0 40 55825.5 41 63605.5 42 72146.5 43 77297.0 44 81071.0 45 85783.0 46 84438.0 47 84016.0 48 82372.0 49 68890.0 50 58913.0 51 48674.0 52 34861.0 53 28124.5 54 24359.0 55 23049.0 56 20485.5 57 18629.0 58 15974.0 59 11193.5 60 8740.0 61 7485.0 62 5951.0 63 4406.0 64 3468.0 65 2693.5 66 1542.0 67 985.0 68 794.0 69 718.0 70 440.5 71 228.0 72 178.5 73 89.0 74 64.5 75 51.5 76 24.0 77 19.0 78 11.0 79 9.0 80 7.0 81 4.0 82 2.0 83 1.5 84 3.0 85 2.5 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 86 1357173.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.869177079281005 #Duplication Level Percentage of deduplicated Percentage of total 1 79.04756352343068 15.70610037332758 2 9.233907853230745 3.6694030053921045 3 3.1428140153496362 1.8733538459468426 4 1.4632184607001806 1.1629178680529946 5 0.9192848523477984 0.9132716758799552 6 0.6143740512259429 0.7324264090034112 7 0.4723708817999307 0.6569934488305258 8 0.3328859984114251 0.5291336679719888 9 0.2532941877122829 0.45294723619271926 >10 2.396531659473748 10.843051386738253 >50 0.8047200035590668 11.619659364113263 >100 1.3047584342952296 48.45189834675168 >500 0.010143529434486381 1.2891324705602165 >1k 0.003756862753513474 1.2566615600394466 >5k 0.0 0.0 >10k+ 3.7568627535134743E-4 0.8430493411990206 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11294 0.8321709907285217 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 3341 0.24617347972587136 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 2493 0.18369065697593454 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA 1854 0.1366074921914892 No Hit GGAATGGGGATTCATTACATATCTTGAATATATACATAATTTTGTATTTT 1707 0.12577615381384688 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1703 0.1254814235178566 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08642965929914609 0.0 2 1.47365147995134E-4 0.0 0.0 0.3074773812918471 0.0 3 1.47365147995134E-4 0.0 0.0 0.43951655389548716 0.0 4 1.47365147995134E-4 0.0 0.0 0.6781744110736067 0.0 5 1.47365147995134E-4 0.0 0.0 1.1364800213384734 0.0 6 1.47365147995134E-4 0.0 0.0 1.512776926744048 0.0 7 1.47365147995134E-4 0.0 0.0 1.7471611946303087 0.0 8 1.47365147995134E-4 0.0 0.0 2.2546867643255504 0.0 9 1.47365147995134E-4 0.0 0.0 2.394094194328947 0.0 10 1.47365147995134E-4 0.0 0.0 2.678803660255546 0.0 11 1.47365147995134E-4 0.0 0.0 3.163413949437544 0.0 12 1.47365147995134E-4 0.0 0.0 3.5117851592980407 0.0 13 1.47365147995134E-4 0.0 0.0 3.6398454729058125 0.0 14 1.47365147995134E-4 0.0 0.0 3.6998967707138295 0.0 15 1.47365147995134E-4 0.0 0.0 3.7820528407211165 0.0 16 1.47365147995134E-4 0.0 0.0 4.001184815789881 0.0 17 1.47365147995134E-4 0.0 0.0 4.259736967947344 0.0 18 1.47365147995134E-4 0.0 0.0 4.568245905275156 0.0 19 1.47365147995134E-4 0.0 0.0 4.757389072726911 0.0 20 1.47365147995134E-4 0.0 0.0 4.939090300204911 0.0 21 1.47365147995134E-4 0.0 0.0 5.195726705438437 0.0 22 1.47365147995134E-4 0.0 0.0 5.4657733391395205 0.0 23 1.47365147995134E-4 0.0 0.0 5.801618511420431 0.0 24 1.47365147995134E-4 0.0 0.0 6.018834739565258 0.0 25 1.47365147995134E-4 0.0 0.0 6.207609494147024 0.0 26 1.47365147995134E-4 0.0 0.0 6.395573740414818 0.0 27 1.47365147995134E-4 0.0 0.0 6.562906865963293 0.0 28 1.47365147995134E-4 0.0 0.0 6.767744421676529 0.0 29 1.47365147995134E-4 0.0 0.0 6.99151839890714 0.0 30 1.47365147995134E-4 0.0 0.0 7.240565499018916 0.0 31 1.47365147995134E-4 0.0 0.0 7.497349269400438 0.0 32 1.47365147995134E-4 0.0 0.0 7.714270767249275 0.0 33 1.47365147995134E-4 0.0 0.0 7.941139412587783 0.0 34 1.47365147995134E-4 0.0 0.0 8.15386100371876 0.0 35 1.47365147995134E-4 0.0 0.0 8.424202367715832 0.0 36 1.47365147995134E-4 0.0 0.0 8.654902506902214 0.0 37 1.47365147995134E-4 0.0 0.0 8.900118113166117 0.0 38 1.47365147995134E-4 0.0 0.0 9.146954736057967 0.0 39 1.47365147995134E-4 0.0 0.0 9.40675949197339 0.0 40 1.47365147995134E-4 0.0 0.0 9.675185109046526 0.0 41 1.47365147995134E-4 0.0 0.0 9.95304209559135 0.0 42 1.47365147995134E-4 0.0 0.0 10.213878407542738 0.0 43 2.2104772199270102E-4 0.0 0.0 10.467493827242363 0.0 44 2.2104772199270102E-4 0.0 0.0 10.72317235901392 0.0 45 2.2104772199270102E-4 0.0 0.0 10.981871876319378 0.0 46 2.2104772199270102E-4 0.0 0.0 11.257518385644277 0.0 47 2.2104772199270102E-4 0.0 0.0 11.539427913758969 0.0 48 2.2104772199270102E-4 0.0 0.0 11.8200848381157 0.0 49 2.2104772199270102E-4 0.0 0.0 12.119530818841813 0.0 50 2.94730295990268E-4 0.0 0.0 12.416913687495994 0.0 51 2.94730295990268E-4 0.0 0.0 12.721370083253941 0.0 52 2.94730295990268E-4 0.0 0.0 13.035626261353563 0.0 53 2.94730295990268E-4 0.0 0.0 13.335661702671656 0.0 54 2.94730295990268E-4 0.0 0.0 13.63687606517371 0.0 55 2.94730295990268E-4 0.0 0.0 13.938753570841742 0.0 56 2.94730295990268E-4 0.0 0.0 14.262735848709044 0.0 57 3.68412869987835E-4 0.0 0.0 14.562771290027138 0.0 58 3.68412869987835E-4 0.0 0.0 14.832891606302217 0.0 59 3.68412869987835E-4 0.0 0.0 15.142063686796009 0.0 60 3.68412869987835E-4 0.0 0.0 15.458382976967565 0.0 61 3.68412869987835E-4 0.0 0.0 15.782144207112873 0.0 62 3.68412869987835E-4 0.0 0.0 16.117694649097793 0.0 63 3.68412869987835E-4 0.0 0.0 16.483233898699723 0.0 64 3.68412869987835E-4 0.0 0.0 16.823794755716477 0.0 65 3.68412869987835E-4 0.0 0.0 17.13989299816604 0.0 66 3.68412869987835E-4 0.0 0.0 17.425634020128605 0.0 67 3.68412869987835E-4 0.0 0.0 17.71830120404694 0.0 68 3.68412869987835E-4 0.0 0.0 18.05790418760173 0.0 69 4.4209544398540203E-4 0.0 0.0 18.406496445184217 0.0 70 4.4209544398540203E-4 0.0 0.0 18.714636969642044 0.0 71 4.4209544398540203E-4 0.0 0.0 19.013861902646163 0.0 72 4.4209544398540203E-4 0.0 0.0 19.31109740615235 0.0 73 5.15778017982969E-4 0.0 0.0 19.61385910270835 0.0 74 5.15778017982969E-4 0.0 0.0 19.969524887394606 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAGCG 20 4.091167E-5 80.0 1 GCGAACG 20 4.091167E-5 80.0 1 ACGTGCG 55 0.0 80.0 1 GCGATAC 30 4.060712E-8 80.0 9 TATAGCG 20 4.091167E-5 80.0 1 TCGGTCG 30 4.060712E-8 80.0 1 CGATTAC 85 0.0 75.29412 9 CGTTTTT 6195 0.0 74.38257 1 CGAACGG 50 3.6379788E-12 72.00001 2 TCTAGCG 50 3.6379788E-12 72.00001 1 AGGGCGA 415 0.0 71.3253 6 GTGTCGA 45 1.1823431E-10 71.11111 9 CGTAAGG 240 0.0 70.0 2 TTGCGAG 110 0.0 69.090904 1 AGGGATT 2130 0.0 68.92019 6 CGAAACG 245 0.0 68.57143 80 CCGTAAG 35 1.1816519E-7 68.57143 1 TGCGATG 35 1.1816519E-7 68.57143 1 GCGTAAG 70 0.0 68.57143 1 TACGCGG 170 0.0 68.2353 2 >>END_MODULE