FastQCFastQC Report
Sat 18 Jun 2016
SRR3549299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644861
Sequences flagged as poor quality0
Sequence length86
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90201.398751048675606No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.10668965870164267No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA6680.10358821513473447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA304.057256E-880.06
CAGCGCG304.057256E-880.01
TACTTCG251.2841647E-680.080
CGCGGGC150.001309528880.04
GCGCGAC204.089538E-580.09
TAGCGCG451.8189894E-1280.01
CACGCAG204.089538E-580.01
TAGCACG550.080.01
ACGGACG204.089538E-580.01
CTTTGCG251.2841647E-680.01
TTACGCG404.1836756E-1180.01
CCGCGCG204.089538E-580.01
TTGCACG150.001309528880.01
GCGCTAG150.001309528880.01
GCGTCCG150.001309528880.01
AATCGCG304.057256E-880.01
TACGTAG150.001309528880.01
GTACGCG451.8189894E-1280.01
CGTTTTT40850.073.6352541
TGCGAAG1150.073.043481
Produced by FastQC (version 0.11.5)