FastQCFastQC Report
Sat 18 Jun 2016
SRR3549298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494563
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26640.5386573601340982No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA11990.24243625180209602No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA8010.16196116571599573No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC6520.1318335581108979No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCA6160.12455440459557224No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA5840.11808404591528278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG404.1836756E-1180.01
TCGTTAG204.0885934E-580.01
TAGCGCG404.1836756E-1180.01
TCTAGCG204.0885934E-580.01
TATTGCG204.0885934E-580.01
TACGCGG204.0885934E-580.02
TGTTACG251.283719E-680.01
GGACGTA150.001309347580.08
AACGAAG251.283719E-680.01
CTACGGG1150.073.043483
ATGACGG950.071.578952
TACGGGA1400.071.428574
CGAGGGA2800.068.571434
TATCGCA700.068.5714380
CGTTTTT15300.067.712421
GGCACCG900.066.666678
ATCGCGG303.7923892E-666.6666642
CGTATGG559.094947E-1265.4545442
CCGATCC2750.065.45454480
CGCTAGG559.094947E-1265.4545442
Produced by FastQC (version 0.11.5)