FastQCFastQC Report
Sat 18 Jun 2016
SRR3549297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662410
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81611.232016424872813No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA27920.42149122144895157No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA26190.39537446596518766No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA15770.23807007744448302No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14450.2181428420464667No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT10430.15745535242523512No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA10300.15549282166633957No Hit
GTTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA9240.13949064778611434No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA7520.11352485620688094No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAAC7120.10748630002566385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG204.0896186E-580.000011
GCGTATG204.0896186E-580.000011
TCGCGAG204.0896186E-580.000011
TCTATCG204.0896186E-580.000011
GCTAGCG600.080.01
TATCGTA351.2896635E-980.01
CCGTTGG150.001309544880.01
GCGTAAG251.2842029E-680.01
ACGTAGG950.075.7894742
GGCGATA800.075.000018
CGTAAGG750.074.6666642
GGCGAGG1400.074.285712
CGTTTTT36500.073.424661
GTAGCGG1700.072.941182
ACGATTG503.6379788E-1272.01
CGGGATA2350.071.489366
AACGGGA1850.071.351364
ACCGGAA900.071.1111154
GAGTACG451.1823431E-1071.1111151
TCGATGG451.1823431E-1071.1111152