##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549297_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	662410
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818779909723585	31.0	31.0	34.0	30.0	34.0
2	32.22733654383237	33.0	31.0	34.0	30.0	34.0
3	32.299793179450795	34.0	31.0	34.0	30.0	34.0
4	35.94574659198985	37.0	35.0	37.0	35.0	37.0
5	35.880839661237	37.0	35.0	37.0	35.0	37.0
6	35.942792228378195	37.0	35.0	37.0	35.0	37.0
7	36.182403647287934	37.0	35.0	37.0	35.0	37.0
8	36.1520025361936	37.0	35.0	37.0	35.0	37.0
9	37.90552527890581	39.0	38.0	39.0	35.0	39.0
10-11	37.5149318397971	39.0	37.0	39.0	35.0	39.0
12-13	36.307696139852965	38.0	35.0	39.0	33.0	39.0
14-15	37.30184251445479	39.0	35.0	41.0	33.0	41.0
16-17	37.54591189746532	39.0	35.0	41.0	33.0	41.0
18-19	37.42368397216226	38.5	35.0	41.0	33.0	41.0
20-21	37.10852644132788	38.0	35.0	41.0	33.0	41.0
22-23	36.84113011578931	38.0	35.0	41.0	32.5	41.0
24-25	36.806158572485316	38.0	35.0	41.0	33.0	41.0
26-27	36.75500067933757	38.0	35.0	41.0	32.5	41.0
28-29	36.79256880179949	38.0	35.0	41.0	33.0	41.0
30-31	36.68170694886852	38.0	35.0	41.0	32.5	41.0
32-33	36.232620280490934	38.0	35.0	41.0	31.0	41.0
34-35	35.90225540073369	38.0	35.0	41.0	30.0	41.0
36-37	35.665492670702434	38.0	35.0	40.5	30.0	41.0
38-39	35.682424027415045	38.0	35.0	40.0	30.0	41.0
40-41	35.56498241270512	37.0	35.0	40.0	29.0	41.0
42-43	35.40669072024879	37.0	35.0	40.0	29.0	41.0
44-45	35.23441373167675	37.0	34.5	40.0	28.0	41.0
46-47	35.13621548587733	37.0	34.0	40.0	28.0	41.0
48-49	35.07194788726015	36.0	34.5	40.0	27.5	41.0
50-51	34.91980269017678	36.0	34.0	40.0	27.0	41.0
52-53	34.72343412690026	36.0	34.0	40.0	27.0	41.0
54-55	34.57603976389245	35.5	34.0	40.0	26.5	41.0
56-57	34.27250871816548	35.0	34.0	39.5	25.5	41.0
58-59	33.99539786537039	35.0	34.0	39.0	25.0	40.5
60-61	33.60659410334989	35.0	33.0	38.5	24.0	40.0
62-63	33.1503064567262	35.0	32.5	38.0	22.5	40.0
64-65	32.87163010824112	35.0	32.0	37.5	22.5	40.0
66-67	32.60104467021935	35.0	32.0	37.0	21.5	39.5
68-69	32.30685451608521	35.0	32.0	36.5	20.5	39.0
70-71	32.06878594827977	35.0	32.0	36.0	20.0	39.0
72-73	31.857500641596594	35.0	32.0	35.5	20.0	38.0
74-75	31.54300206820549	35.0	32.0	35.0	19.0	37.5
76-77	29.541515828565387	32.5	28.5	34.5	16.0	35.5
78-79	30.424671276097882	34.0	30.0	35.0	17.0	36.0
80-81	30.816655847586844	34.0	31.0	35.0	18.0	36.0
82-83	30.645785842605036	34.0	31.0	35.0	16.5	36.0
84-85	30.461975211726877	34.0	31.0	35.0	15.0	35.0
86	30.095991908334717	34.0	30.0	35.0	14.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	7.0
12	8.0
13	28.0
14	88.0
15	308.0
16	785.0
17	1655.0
18	3344.0
19	5769.0
20	7313.0
21	7503.0
22	6880.0
23	5959.0
24	6076.0
25	6465.0
26	7238.0
27	8750.0
28	10698.0
29	13192.0
30	16503.0
31	20557.0
32	25609.0
33	34698.0
34	94071.0
35	79701.0
36	50364.0
37	89042.0
38	116957.0
39	42773.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.15830075029061	24.420826980269016	25.20010265545508	16.220769613985297
2	33.98574900741233	25.14756721667849	27.478751830437343	13.387931945471838
3	29.52944550957866	24.689995622046766	32.590087710028534	13.190471158346039
4	29.338929062061258	25.40496067390287	30.99424827523739	14.261861988798477
5	22.65636086411739	33.44152413158014	29.215440588155374	14.686674416147099
6	20.40216784166906	42.31095545055177	27.164746909014053	10.122129798765116
7	83.36181518998808	5.54641385244788	9.411542700140396	1.68022825742365
8	83.35728627285216	5.146661433251309	8.85403300070953	2.642019293186999
9	78.27659606588064	7.627904168113404	10.843586298516025	3.2519134674899233
10-11	45.618574598813424	24.627421083618906	19.273108799685996	10.480895517881676
12-13	31.639618967104965	30.716852100662734	24.6053048716052	13.038224060627105
14-15	14.443848975709908	35.66054256427289	36.968191905315436	12.92741655470177
16-17	16.717969233556257	22.299859603568787	37.6799867151764	23.302184447698554
18-19	24.008544556996423	26.984646970909253	31.256849987168067	17.749958484926253
20-21	26.77065563623738	26.793526667773737	29.091574704488153	17.34424299150073
22-23	19.977204450415904	26.758050150209083	29.378179677239174	23.88656572213584
24-25	18.036337011820475	26.86644223366193	37.83019580018417	17.26702495433342
26-27	16.503826934979845	36.903428390271884	29.855376579459854	16.737368095288417
28-29	13.711598556785074	27.873673404688937	41.23028033997072	17.184447698555275
30-31	22.831780921181746	29.62447728748056	30.83611358524177	16.707628206095922
32-33	25.722286801225824	31.027686780090878	26.621654262465842	16.62837215621745
34-35	17.710707869748344	30.424887909300885	29.795217463504475	22.069186757446293
36-37	21.83617397080358	30.147491734726227	31.18944460379523	16.82688969067496
38-39	17.67606165365861	34.50438550142661	28.086985401790432	19.73256744312435
40-41	19.928820519013904	28.308147521927506	28.58327923793421	23.17975272112438
42-43	21.80937787774943	26.34116332784831	27.815778747301522	24.033680047100738
44-45	17.56751860630123	35.60000603855618	27.707235699944142	19.125239655198442
46-47	21.41022931417098	31.868933138086685	26.845382768979935	19.875454778762396
48-49	20.054799897344544	26.58164882776528	32.93073776060144	20.432813514288732
50-51	17.301444724566355	32.98214097009405	29.18992768828973	20.526486617049862
52-53	22.678929967844688	26.69555109373349	30.026267719388294	20.59925121903353
54-55	22.572877824912062	27.318654609682824	31.590555698132576	18.517911867272534
56-57	23.096873537537174	24.351987439803143	29.66176537189958	22.889373650760103
58-59	24.924140637973462	25.78976766655093	29.036774807143612	20.249316888332
60-61	24.962862879485513	31.45332950891442	25.893857278724656	17.68995033287541
62-63	24.804652707537628	26.58451714195136	29.88330490179798	18.727525248713032
64-65	18.490285472743466	33.20760556151024	31.081958303769568	17.22015066197672
66-67	16.021270814148338	30.99681466161441	25.206367657493097	27.77554686674416
68-69	16.280702284083876	34.356667320843584	24.448453374798085	24.91417702027445
70-71	18.468395706586556	31.43974275750668	28.804894249784873	21.28696728612189
72-73	23.252366359203513	31.75948430730212	24.98075210217237	20.007397231321992
74-75	21.382074545976057	34.67338959254842	22.60888271614257	21.33565314533295
76-77	28.587506227261063	29.30654730453948	24.34828882414215	17.757657644057307
78-79	17.379568545160854	32.65100164550656	29.97607222113193	19.99335758820066
80-81	14.443547047900848	30.53584637913075	31.23194094292055	23.788665630047856
82-83	18.87977234643197	26.005268640268113	32.06284627345602	23.052112739843903
84-85	15.601817605410545	26.8489304207364	29.034359384671127	28.51489258918193
86	17.04624024395767	26.21563684123126	33.26912335260639	23.468999562204676
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	503.0
1	592.5
2	3014.0
3	3525.0
4	1627.0
5	1620.5
6	1780.5
7	1870.0
8	1871.5
9	1814.0
10	1782.0
11	1741.0
12	1715.5
13	1793.0
14	1828.0
15	1849.5
16	1928.5
17	1926.5
18	2121.5
19	2408.5
20	2414.0
21	2387.0
22	2314.0
23	2334.5
24	2523.0
25	3029.0
26	4320.5
27	5618.0
28	7664.5
29	9294.0
30	13666.0
31	17251.5
32	13381.0
33	10880.5
34	12304.0
35	13738.5
36	14333.0
37	14209.5
38	14839.0
39	17385.5
40	21181.0
41	26288.0
42	32060.5
43	34728.0
44	38991.5
45	40926.5
46	43190.5
47	48091.5
48	45965.5
49	37430.5
50	31330.0
51	26003.5
52	19404.5
53	16366.0
54	13979.5
55	12750.0
56	11442.5
57	10744.0
58	9820.0
59	7186.0
60	4576.0
61	3684.0
62	3728.5
63	3241.0
64	2717.0
65	2041.5
66	1026.0
67	597.0
68	440.0
69	282.5
70	146.0
71	99.0
72	98.0
73	97.0
74	61.5
75	27.0
76	22.5
77	13.5
78	6.5
79	3.0
80	2.0
81	0.5
82	0.5
83	1.0
84	1.5
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	662410.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.65395465070606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.87868902483916	15.273613519286137
2	7.935686803161667	2.960638834967686
3	2.4132675595727133	1.3505095084886842
4	1.1391704046292888	0.8500013226952491
5	0.6722657625664166	0.6270207524068132
6	0.4506981808933793	0.5044382056524488
7	0.32863683674923266	0.4291263654490174
8	0.23345738015933576	0.3483922705891915
9	0.1911575836681971	0.32092604071965886
>10	2.224745966486126	10.083740698096381
>50	0.9988987094934325	13.734553886487944
>100	1.5203108208821052	49.80158495250991
>500	0.007320918880652193	0.8829627030378759
>1k	0.0048806125871014615	1.5941581299391518
>5k	8.13435431183577E-4	1.238332809673845
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8161	1.232016424872813	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2792	0.42149122144895157	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2619	0.39537446596518766	No Hit
GAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1577	0.23807007744448302	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1445	0.2181428420464667	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1043	0.15745535242523512	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1030	0.15549282166633957	No Hit
GTTGTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	924	0.13949064778611434	No Hit
GGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	752	0.11352485620688094	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAAC	712	0.10748630002566385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0659712262797965	0.0
2	0.0	0.0	0.0	0.2187466976645884	0.0
3	0.0	0.0	0.0	0.30192780906085354	0.0
4	0.0	0.0	0.0	0.4414184568469679	0.0
5	0.0	0.0	0.0	0.7578388007427425	0.0
6	0.0	0.0	0.0	0.9734152564121918	0.0
7	0.0	0.0	0.0	1.119548315997645	0.0
8	0.0	0.0	0.0	1.4220799806766202	0.0
9	0.0	0.0	0.0	1.5045062725502332	0.0
10	1.5096390453042677E-4	0.0	0.0	1.6805301852327108	0.0
11	1.5096390453042677E-4	0.0	0.0	1.9634365423227307	0.0
12	1.5096390453042677E-4	0.0	0.0	2.192448785495388	0.0
13	1.5096390453042677E-4	0.0	0.0	2.272610618801045	0.0
14	1.5096390453042677E-4	0.0	0.0	2.309445811506469	0.0
15	1.5096390453042677E-4	0.0	0.0	2.382361377394665	0.0
16	1.5096390453042677E-4	0.0	0.0	2.51853081928111	0.0
17	1.5096390453042677E-4	0.0	0.0	2.6716082184749625	0.0
18	1.5096390453042677E-4	0.0	0.0	2.904847450974472	0.0
19	1.5096390453042677E-4	0.0	0.0	3.0065971226279795	0.0
20	1.5096390453042677E-4	0.0	0.0	3.1282740296795035	0.0
21	1.5096390453042677E-4	0.0	0.0	3.286937093340982	0.0
22	1.5096390453042677E-4	0.0	0.0	3.43125858607207	0.0
23	1.5096390453042677E-4	0.0	0.0	3.6057728597092433	0.0
24	1.5096390453042677E-4	0.0	0.0	3.729714225328724	0.0
25	1.5096390453042677E-4	0.0	0.0	3.835087030690962	0.0
26	1.5096390453042677E-4	0.0	0.0	3.945441644902704	0.0
27	1.5096390453042677E-4	0.0	0.0	4.044624930179194	0.0
28	1.5096390453042677E-4	0.0	0.0	4.16328255914011	0.0
29	1.5096390453042677E-4	0.0	0.0	4.281940188101025	0.0
30	1.5096390453042677E-4	0.0	0.0	4.444528313280295	0.0
31	1.5096390453042677E-4	0.0	0.0	4.604399088178017	0.0
32	1.5096390453042677E-4	0.0	0.0	4.74011563835087	0.0
33	1.5096390453042677E-4	0.0	0.0	4.868887848915325	0.0
34	1.5096390453042677E-4	0.0	0.0	4.996603312148065	0.0
35	1.5096390453042677E-4	0.0	0.0	5.191950604610438	0.0
36	1.5096390453042677E-4	0.0	0.0	5.354991621503299	0.0
37	1.5096390453042677E-4	0.0	0.0	5.510031551456047	0.0
38	1.5096390453042677E-4	0.0	0.0	5.652088585619178	0.0
39	1.5096390453042677E-4	0.0	0.0	5.797919717395571	0.0
40	1.5096390453042677E-4	0.0	0.0	5.96730121827871	0.0
41	1.5096390453042677E-4	0.0	0.0	6.127021029271901	0.0
42	1.5096390453042677E-4	0.0	0.0	6.292024576923658	0.0
43	1.5096390453042677E-4	0.0	0.0	6.438006672604581	0.0
44	1.5096390453042677E-4	0.0	0.0	6.598632267024954	0.0
45	1.5096390453042677E-4	0.0	0.0	6.760918464395163	0.0
46	1.5096390453042677E-4	0.0	0.0	6.943131897163388	0.0
47	1.5096390453042677E-4	0.0	0.0	7.140743648193717	0.0
48	1.5096390453042677E-4	0.0	0.0	7.319333947253212	0.0
49	1.5096390453042677E-4	0.0	0.0	7.5201159402786795	0.0
50	1.5096390453042677E-4	0.0	0.0	7.733729865189233	0.0
51	1.5096390453042677E-4	0.0	0.0	7.919113539952598	0.0
52	1.5096390453042677E-4	0.0	0.0	8.145408432843707	0.0
53	1.5096390453042677E-4	0.0	0.0	8.339849941878898	0.0
54	1.5096390453042677E-4	0.0	0.0	8.552256155553208	0.0
55	1.5096390453042677E-4	0.0	0.0	8.793194547183768	0.0
56	1.5096390453042677E-4	0.0	0.0	9.026735707492339	0.0
57	1.5096390453042677E-4	0.0	0.0	9.230235050799354	0.0
58	1.5096390453042677E-4	0.0	0.0	9.427544874020622	0.0
59	1.5096390453042677E-4	0.0	0.0	9.630591325614045	0.0
60	1.5096390453042677E-4	0.0	0.0	9.849790914992225	0.0
61	1.5096390453042677E-4	0.0	0.0	10.07397231321991	0.0
62	1.5096390453042677E-4	0.0	0.0	10.328497456258209	0.0
63	1.5096390453042677E-4	0.0	0.0	10.64386105282227	0.0
64	1.5096390453042677E-4	0.0	0.0	10.908198849655047	0.0
65	1.5096390453042677E-4	0.0	0.0	11.131776392264609	0.0
66	1.5096390453042677E-4	0.0	0.0	11.342522002989085	0.0
67	1.5096390453042677E-4	0.0	0.0	11.546625201914223	0.0
68	1.5096390453042677E-4	0.0	0.0	11.77926057879561	0.0
69	1.5096390453042677E-4	0.0	0.0	12.036352108210927	0.0
70	1.5096390453042677E-4	0.0	0.0	12.238039884663577	0.0
71	1.5096390453042677E-4	0.0	0.0	12.446219109011036	0.0
72	1.5096390453042677E-4	0.0	0.0	12.685345933787232	0.0
73	1.5096390453042677E-4	0.0	0.0	12.931266134267297	0.0
74	1.5096390453042677E-4	0.0	0.0	13.205869476608143	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	20	4.0896186E-5	80.00001	1
GCGTATG	20	4.0896186E-5	80.00001	1
TCGCGAG	20	4.0896186E-5	80.00001	1
TCTATCG	20	4.0896186E-5	80.00001	1
GCTAGCG	60	0.0	80.0	1
TATCGTA	35	1.2896635E-9	80.0	1
CCGTTGG	15	0.0013095448	80.0	1
GCGTAAG	25	1.2842029E-6	80.0	1
ACGTAGG	95	0.0	75.789474	2
GGCGATA	80	0.0	75.00001	8
CGTAAGG	75	0.0	74.666664	2
GGCGAGG	140	0.0	74.28571	2
CGTTTTT	3650	0.0	73.42466	1
GTAGCGG	170	0.0	72.94118	2
ACGATTG	50	3.6379788E-12	72.0	1
CGGGATA	235	0.0	71.48936	6
AACGGGA	185	0.0	71.35136	4
ACCGGAA	90	0.0	71.111115	4
GAGTACG	45	1.1823431E-10	71.111115	1
TCGATGG	45	1.1823431E-10	71.111115	2
>>END_MODULE