FastQCFastQC Report
Sat 18 Jun 2016
SRR3549296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875437
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60550.691654567947208No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20840.23805253833228432No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA15450.17648328777513403No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14980.1711145405094827No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA14900.17020071118766972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9360.10691803065212002No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTT9100.1039480853562278No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC404.1836756E-1180.000019
TTCGCGA204.0903542E-580.000011
TCGTTAG150.001309686180.01
GCGATAA304.059075E-880.09
CGATTGG850.075.294112
CGTTTTT29900.073.043471
TTAGCGG1700.072.941182
ACGGGTA550.072.727285
ATTAACG503.6379788E-1272.01
CTACGCG451.1823431E-1071.111111
TAAGGGA8200.070.731714
ATGCGCG403.7125574E-970.000011
GCATAGG3150.069.841272
TACGCGG700.068.571432
AGGGATT9900.067.07076
GGTCGTT303.7948284E-666.6666648
CGTTAGG600.066.6666642
CGGGGGA10900.066.422016
TTTGCGG3200.066.250012
ACGGGAT4350.066.2068945
Produced by FastQC (version 0.11.5)