##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549296_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	875437
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752909689674986	31.0	31.0	33.0	30.0	34.0
2	32.16236348246647	33.0	31.0	34.0	30.0	34.0
3	32.27110117575565	34.0	31.0	34.0	30.0	34.0
4	35.89303742016844	37.0	35.0	37.0	35.0	37.0
5	35.84497228241438	37.0	35.0	37.0	35.0	37.0
6	35.879312846041465	37.0	35.0	37.0	35.0	37.0
7	36.15659607715918	37.0	35.0	37.0	35.0	37.0
8	36.14063262119376	37.0	35.0	37.0	35.0	37.0
9	37.925580024604855	39.0	38.0	39.0	35.0	39.0
10-11	37.40277198701905	39.0	37.0	39.0	34.5	39.0
12-13	36.137943107271	38.0	35.0	39.0	32.5	39.0
14-15	37.11275740001851	39.0	35.0	41.0	33.0	41.0
16-17	37.386678310375274	39.0	35.0	41.0	33.0	41.0
18-19	37.318250199614596	38.0	35.0	41.0	33.0	41.0
20-21	37.0529004371531	38.0	35.0	41.0	33.0	41.0
22-23	36.7876728993634	38.0	35.0	41.0	32.0	41.0
24-25	36.730889829879246	38.0	35.0	41.0	32.0	41.0
26-27	36.625839437903586	38.0	35.0	41.0	32.0	41.0
28-29	36.64708711192239	38.0	35.0	41.0	32.0	41.0
30-31	36.52396517396454	38.0	35.0	40.5	32.0	41.0
32-33	36.16249141857152	37.0	35.0	40.0	31.0	41.0
34-35	35.892867790600576	37.0	35.0	40.0	30.0	41.0
36-37	35.64614186971764	37.0	35.0	40.0	30.0	41.0
38-39	35.666467718408065	37.0	35.0	40.0	30.0	41.0
40-41	35.55377828444537	37.0	35.0	40.0	30.0	41.0
42-43	35.42063506568719	36.5	35.0	40.0	29.5	41.0
44-45	35.2445481513804	36.0	34.5	40.0	29.0	41.0
46-47	35.15448798714242	36.0	34.0	40.0	29.0	41.0
48-49	35.03999259798249	36.0	34.0	40.0	28.0	41.0
50-51	34.89308939421112	35.5	34.0	40.0	28.0	41.0
52-53	34.676226273278374	35.5	34.0	40.0	27.0	41.0
54-55	34.52647135087962	35.0	34.0	39.5	27.0	41.0
56-57	34.224277703592605	35.0	34.0	39.0	25.5	41.0
58-59	33.94685225778669	35.0	34.0	39.0	25.5	40.0
60-61	33.52364761827521	35.0	33.0	38.0	24.5	40.0
62-63	33.01073178309804	35.0	32.5	37.5	23.0	40.0
64-65	32.692916794697965	35.0	32.0	37.0	22.5	40.0
66-67	32.336190382631756	35.0	32.0	36.5	21.0	39.0
68-69	31.975910887933683	35.0	32.0	36.0	19.5	39.0
70-71	31.69398369043118	35.0	31.5	35.5	19.0	38.5
72-73	31.435307166592228	35.0	31.0	35.0	18.0	37.5
74-75	31.138887778332425	34.5	31.0	35.0	17.5	37.0
76-77	29.167086837773592	32.5	28.0	34.5	12.5	35.5
78-79	29.995724992203897	33.5	29.5	35.0	14.0	36.0
80-81	30.41173094123278	34.0	30.5	35.0	15.0	36.0
82-83	30.262464346377868	34.0	30.0	35.0	11.0	35.5
84-85	30.08431560466373	34.0	30.0	35.0	10.0	35.0
86	29.727572629441067	34.0	29.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	9.0
12	14.0
13	46.0
14	169.0
15	424.0
16	1008.0
17	2195.0
18	4189.0
19	6631.0
20	7931.0
21	8073.0
22	7642.0
23	7203.0
24	7965.0
25	9164.0
26	11162.0
27	13465.0
28	16322.0
29	20137.0
30	24983.0
31	31310.0
32	37785.0
33	50353.0
34	133451.0
35	110069.0
36	66966.0
37	110820.0
38	136858.0
39	49018.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.250834725971142	25.254130222962935	29.90860564495218	14.586429406113746
2	28.963820354862772	27.853175042864308	30.039625923967115	13.143378678305806
3	28.649006153498195	25.465795939627867	32.652263955030456	13.232933951843478
4	26.85070427683545	28.357951514500755	31.085160896786405	13.706183311877382
5	24.761233532510047	31.88955915731229	29.71099005411012	13.638217256067541
6	20.750436639072827	41.44592929017165	26.503677591876972	11.29995647887855
7	85.9938522132375	4.95786675683116	7.068698261553943	1.9795827683773932
8	87.18959788082981	4.163634847510443	6.451178097338815	2.195589174320939
9	82.7228001558079	6.5450740601551	7.891373108516089	2.8407526755209114
10-11	49.306860459404845	23.345769027354336	16.99368429709962	10.353686216141195
12-13	31.211954715187957	31.831645224042393	23.123651387821166	13.83274867294848
14-15	13.916249827229144	35.325100492668234	37.29137562154673	13.467274058555898
16-17	15.839003834656292	22.99645776909132	36.142292363699504	25.022246032552886
18-19	24.744556147386962	28.032628275935334	28.82531809827549	18.397497478402215
20-21	26.494996213319748	28.610339750318985	26.272478773458285	18.622185262902985
22-23	19.565542694677056	28.574700406768276	27.162091618243235	24.697665280311433
24-25	17.536099113928245	27.716214873257584	35.96392430294813	18.783761709866045
26-27	16.795097762603135	38.01718456039669	27.873736202605098	17.313981474395074
28-29	14.053724025829387	29.79609040970395	38.0987438273685	18.05144173709816
30-31	23.671434951915444	30.628360464545135	27.92256895698948	17.77763562654994
32-33	27.071908087046815	30.41646629054975	25.220375652388466	17.291249970014974
34-35	18.47985634603061	29.943616730844134	28.935263188556114	22.641263734569137
36-37	23.202469166827537	30.3909361838716	29.479334321030525	16.92726032827034
38-39	18.49584835916234	34.1171894722293	26.984523158148445	20.40243901045992
40-41	19.998869136214257	28.55493884768407	26.889370680014668	24.556821336087005
42-43	22.007523099891827	26.545599512015144	26.06669583305252	25.380181555040508
44-45	17.627196474446478	36.5884695300747	25.757821522279727	20.026512473199098
46-47	21.72652058343433	32.172503561078635	25.374355893113954	20.72661996237308
48-49	20.822172240835148	26.458328811782	31.935878881061686	20.783620066321163
50-51	17.913110823508717	34.41041445586604	27.21412277525396	20.46235194537128
52-53	23.277460285548816	26.532863015842373	29.318329017393594	20.87134768121521
54-55	22.98903290585159	28.655345844418274	29.366190828123557	18.98943042160658
56-57	23.393116809090774	24.14594082726684	29.033785412314078	23.427156951328307
58-59	24.952680775429872	27.646478273136733	27.074249774683956	20.32659117674944
60-61	25.985993281069913	31.87196794286739	24.191917865020553	17.95012091104214
62-63	24.51529921627713	27.4035139021997	29.277263812244627	18.803923069278543
64-65	17.59052907290873	34.04733864344322	30.450849118782962	17.91128316486509
66-67	16.059750730206744	31.602274064267334	23.90554660129741	28.432428604228516
68-69	16.875286285592225	35.534138950032954	22.29280919129532	25.297765573079502
70-71	18.206221578480232	33.021451001042905	27.258043697033596	21.514283723443263
72-73	23.22285898357049	33.26018891136655	24.158791552104837	19.35816055295812
74-75	22.48185763224538	34.82632102595618	21.34813812987114	21.3436832119273
76-77	29.14430164592084	28.8129242880984	23.61809016525461	18.42468390072615
78-79	17.769239819655784	31.484961225079587	30.400759849081087	20.345039106183542
80-81	14.834362723988134	29.824704690343225	30.691985831076362	24.648946754592277
82-83	17.614231520943253	27.14375791747436	32.03091713053024	23.21109343105215
84-85	15.16659679679977	27.215950433897586	29.207755669454226	28.40969709984842
86	17.033778558594165	27.28214594539641	32.65374892767841	23.030326568331017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	677.0
1	767.5
2	2412.0
3	2563.5
4	1294.5
5	1733.0
6	2322.0
7	2606.0
8	2705.0
9	2665.5
10	2343.5
11	2049.0
12	1946.5
13	1961.0
14	1967.0
15	2003.0
16	2102.5
17	2249.5
18	2325.5
19	2431.5
20	2600.0
21	2655.0
22	2779.5
23	3133.0
24	3543.0
25	4001.0
26	4265.0
27	5673.5
28	8767.5
29	10440.0
30	11405.5
31	15846.0
32	17561.0
33	13946.0
34	13583.0
35	15850.0
36	16625.0
37	17791.0
38	20352.5
39	24496.0
40	30078.0
41	37790.0
42	46929.5
43	51191.0
44	55033.0
45	59572.0
46	62522.0
47	65614.5
48	62519.5
49	50656.5
50	42728.0
51	35741.0
52	25740.5
53	20675.0
54	17901.0
55	17692.5
56	16681.5
57	15157.0
58	12715.0
59	8778.0
60	6178.0
61	5010.5
62	4485.5
63	3480.5
64	2938.0
65	2289.5
66	1548.0
67	1222.0
68	859.0
69	551.0
70	390.5
71	408.0
72	363.0
73	235.0
74	118.5
75	73.0
76	70.0
77	51.0
78	15.5
79	8.0
80	14.0
81	16.5
82	7.5
83	3.0
84	3.5
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	875437.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.885596089639048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1973340292543	17.332808639404853
2	7.891980008762022	3.4544137363854333
3	2.5892680253617195	1.700030225126516
4	1.3669360805533166	1.1966484375737676
5	0.8521285617189212	0.9324670759112683
6	0.5855860869494167	0.7689540344812311
7	0.4341658282646326	0.6651384567326339
8	0.3807856734566417	0.6666977156794599
9	0.30956479403808035	0.6097509041300755
>10	3.922381914210028	21.02023950531185
>50	1.7014417884595239	26.795168510774236
>100	0.7620765708807777	22.79070698521715
>500	0.0037045388862260026	0.5992677715402269
>1k	0.002116879363557716	0.7664002404873757
>5k	5.29219840889429E-4	0.7013077612439262
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6055	0.691654567947208	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2084	0.23805253833228432	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1545	0.17648328777513403	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1498	0.1711145405094827	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1490	0.17020071118766972	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	936	0.10691803065212002	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTT	910	0.1039480853562278	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09229676150311215	0.0
2	0.0	0.0	0.0	0.32201060727385294	0.0
3	0.0	0.0	0.0	0.45314511495401727	0.0
4	0.0	0.0	0.0	0.6981656018651257	0.0
5	0.0	0.0	0.0	1.1823809137607846	0.0
6	0.0	0.0	0.0	1.7214259849652231	0.0
7	0.0	0.0	0.0	2.044921564887022	0.0
8	0.0	0.0	0.0	2.6398244533872797	0.0
9	0.0	0.0	0.0	2.8244179763935042	0.0
10	0.0	0.0	0.0	3.142773266380105	0.0
11	0.0	0.0	0.0	3.5963752959950286	0.0
12	0.0	0.0	0.0	3.9736725772385677	0.0
13	0.0	0.0	0.0	4.117372238093661	0.0
14	0.0	0.0	0.0	4.174372342041746	0.0
15	0.0	0.0	0.0	4.255588923017876	0.0
16	0.0	0.0	0.0	4.43070146681029	0.0
17	0.0	0.0	0.0	4.642595640805678	0.0
18	0.0	0.0	0.0	4.915944836692988	0.0
19	0.0	0.0	0.0	5.076321882671169	0.0
20	0.0	0.0	0.0	5.227332178100766	0.0
21	0.0	0.0	0.0	5.437855608113433	0.0
22	0.0	0.0	0.0	5.660258819309671	0.0
23	0.0	0.0	0.0	5.894199125693796	0.0
24	0.0	0.0	0.0	6.069083212155758	0.0
25	0.0	0.0	0.0	6.213696702332664	0.0
26	0.0	0.0	0.0	6.353626817235278	0.0
27	0.0	0.0	0.0	6.477108004345259	0.0
28	0.0	0.0	0.0	6.6211503511960315	0.0
29	0.0	0.0	0.0	6.787695745096449	0.0
30	0.0	0.0	0.0	6.9826840766382965	0.0
31	0.0	0.0	0.0	7.16864834362724	0.0
32	0.0	0.0	0.0	7.33073881958382	0.0
33	0.0	0.0	0.0	7.49008780757496	0.0
34	0.0	0.0	0.0	7.6493225669008735	0.0
35	0.0	0.0	0.0	7.862130570218074	0.0
36	0.0	0.0	0.0	8.053235127142216	0.0
37	0.0	0.0	0.0	8.231546073560976	0.0
38	0.0	0.0	0.0	8.3985483821223	0.0
39	0.0	0.0	0.0	8.575945499219246	0.0
40	0.0	0.0	0.0	8.77241880340904	1.1422866522662397E-4
41	0.0	0.0	0.0	8.965236790311582	1.1422866522662397E-4
42	0.0	0.0	0.0	9.163651981810228	1.1422866522662397E-4
43	0.0	0.0	0.0	9.33979258358968	1.1422866522662397E-4
44	0.0	0.0	0.0	9.52461456392636	1.1422866522662397E-4
45	0.0	0.0	0.0	9.722572840764098	1.1422866522662397E-4
46	0.0	0.0	0.0	9.934467014759486	1.1422866522662397E-4
47	0.0	0.0	0.0	10.141220898819675	1.1422866522662397E-4
48	0.0	0.0	0.0	10.339864547648775	1.1422866522662397E-4
49	0.0	0.0	0.0	10.556442096918452	1.1422866522662397E-4
50	0.0	0.0	0.0	10.792781205272338	1.1422866522662397E-4
51	0.0	0.0	0.0	10.989939881453491	1.1422866522662397E-4
52	0.0	0.0	0.0	11.20686011671885	1.1422866522662397E-4
53	0.0	0.0	0.0	11.415098973426986	1.1422866522662397E-4
54	0.0	0.0	0.0	11.635103382653464	1.1422866522662397E-4
55	0.0	0.0	0.0	11.862075740458765	1.1422866522662397E-4
56	0.0	0.0	0.0	12.10047096478673	1.1422866522662397E-4
57	0.0	0.0	0.0	12.317391200052088	1.1422866522662397E-4
58	0.0	0.0	0.0	12.520603995490251	1.1422866522662397E-4
59	0.0	0.0	0.0	12.740151490055824	1.1422866522662397E-4
60	0.0	0.0	0.0	12.965753103878406	1.1422866522662397E-4
61	0.0	0.0	0.0	13.197408836958	1.1422866522662397E-4
62	0.0	0.0	0.0	13.446427327152039	1.1422866522662397E-4
63	0.0	0.0	0.0	13.792768640119164	1.1422866522662397E-4
64	0.0	0.0	0.0	14.05412382615768	1.1422866522662397E-4
65	0.0	0.0	0.0	14.296631282433802	1.1422866522662397E-4
66	0.0	0.0	0.0	14.498130647893566	1.1422866522662397E-4
67	0.0	0.0	0.0	14.702485729983996	1.1422866522662397E-4
68	0.0	0.0	0.0	14.939053295668336	1.1422866522662397E-4
69	1.1422866522662397E-4	0.0	0.0	15.18658681321443	1.1422866522662397E-4
70	1.1422866522662397E-4	0.0	0.0	15.394140297931205	1.1422866522662397E-4
71	1.1422866522662397E-4	0.0	0.0	15.634134723572341	1.1422866522662397E-4
72	1.1422866522662397E-4	0.0	0.0	15.847742327546129	1.1422866522662397E-4
73	2.2845733045324793E-4	0.0	0.0	16.08248223458684	1.1422866522662397E-4
74	2.2845733045324793E-4	0.0	0.0	16.332071868107015	1.1422866522662397E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	40	4.1836756E-11	80.00001	9
TTCGCGA	20	4.0903542E-5	80.00001	1
TCGTTAG	15	0.0013096861	80.0	1
GCGATAA	30	4.059075E-8	80.0	9
CGATTGG	85	0.0	75.29411	2
CGTTTTT	2990	0.0	73.04347	1
TTAGCGG	170	0.0	72.94118	2
ACGGGTA	55	0.0	72.72728	5
ATTAACG	50	3.6379788E-12	72.0	1
CTACGCG	45	1.1823431E-10	71.11111	1
TAAGGGA	820	0.0	70.73171	4
ATGCGCG	40	3.7125574E-9	70.00001	1
GCATAGG	315	0.0	69.84127	2
TACGCGG	70	0.0	68.57143	2
AGGGATT	990	0.0	67.0707	6
GGTCGTT	30	3.7948284E-6	66.666664	8
CGTTAGG	60	0.0	66.666664	2
CGGGGGA	1090	0.0	66.42201	6
TTTGCGG	320	0.0	66.25001	2
ACGGGAT	435	0.0	66.206894	5
>>END_MODULE