FastQCFastQC Report
Sat 18 Jun 2016
SRR3549294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136854
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112860.3597872263101821No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53750.17135002139085848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1300.045.0000041
TAGCGAT207.0351537E-445.00000425
GCTAACG950.042.631581
ACGGGTA3950.042.15195
AGCGTTG1150.041.0869561
TCATGCA6050.040.53719324
TCGTTAG1850.040.1351361
CGAATTA451.9301297E-840.043
CGGGTAT3000.039.756
CGTTTTT82100.039.6833151
GCGATAA2400.039.3750049
TAACGCC1100.038.86363612
CGTAAGG5100.038.823532
CGCACAT356.2517465E-638.57142634
TCGACAG356.2517465E-638.5714261
TATGGGC11700.038.4615364
CGGTCTA1700.038.3823531
TACGAAT3050.038.36065712
TAGGGCG14500.038.3275835
GGGCGAT72050.038.1922267
Produced by FastQC (version 0.11.5)