##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549294_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3136854
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446732936885173	31.0	31.0	33.0	30.0	34.0
2	31.845107231640362	31.0	31.0	34.0	30.0	34.0
3	31.982446425622616	33.0	31.0	34.0	30.0	34.0
4	35.66245926651352	37.0	35.0	37.0	33.0	37.0
5	35.54726710264488	37.0	35.0	37.0	33.0	37.0
6	35.600757638066675	37.0	35.0	37.0	33.0	37.0
7	35.99068270311592	37.0	35.0	37.0	35.0	37.0
8	36.00698789296537	37.0	35.0	37.0	35.0	37.0
9	37.756963186683215	39.0	38.0	39.0	35.0	39.0
10	37.1676810587933	39.0	37.0	39.0	33.0	39.0
11	36.871028744085635	39.0	37.0	39.0	33.0	39.0
12	36.08919987987965	37.0	35.0	39.0	32.0	39.0
13	35.7041360547861	37.0	35.0	39.0	31.0	39.0
14	36.72778586443615	39.0	35.0	41.0	31.0	41.0
15	37.00699713789676	39.0	35.0	41.0	32.0	41.0
16	37.156152629354125	39.0	35.0	41.0	32.0	41.0
17	37.08705601217016	38.0	35.0	41.0	32.0	41.0
18	37.02654857382588	38.0	35.0	41.0	32.0	41.0
19	36.984786349635655	38.0	35.0	41.0	32.0	41.0
20	36.862220237218565	38.0	35.0	41.0	32.0	41.0
21	36.7105507620055	38.0	35.0	41.0	31.0	41.0
22	36.58370839063597	38.0	35.0	41.0	31.0	41.0
23	36.51301845734612	38.0	35.0	40.0	31.0	41.0
24	36.38002597506929	38.0	35.0	40.0	31.0	41.0
25	36.235696019005026	38.0	35.0	40.0	31.0	41.0
26	36.03604949417473	37.0	35.0	40.0	30.0	41.0
27	35.983874926917224	37.0	35.0	40.0	30.0	41.0
28	36.0510693197707	37.0	35.0	40.0	30.0	41.0
29	36.062644611448285	37.0	35.0	40.0	30.0	41.0
30	36.11074088880133	37.0	35.0	40.0	30.0	41.0
31	35.96314396525946	37.0	35.0	40.0	30.0	41.0
32	35.75856479134828	37.0	35.0	40.0	30.0	41.0
33	35.62021630589119	37.0	34.0	40.0	29.0	41.0
34	35.473810065753774	37.0	34.0	40.0	29.0	41.0
35	35.35386568836165	37.0	34.0	40.0	29.0	41.0
36	35.187764237672525	37.0	34.0	40.0	27.0	41.0
37	34.87710330158815	36.0	34.0	40.0	26.0	41.0
38	34.96944454539484	36.0	34.0	40.0	27.0	41.0
39	34.86176914832504	36.0	34.0	40.0	26.0	41.0
40	34.68591174469707	36.0	34.0	40.0	26.0	41.0
41	34.64518240249626	36.0	34.0	40.0	26.0	41.0
42	34.631973945870605	36.0	34.0	40.0	26.0	41.0
43	34.554459021682234	35.0	34.0	40.0	26.0	41.0
44	34.41072297276188	35.0	34.0	40.0	25.0	41.0
45	34.3524751231648	35.0	34.0	40.0	25.0	41.0
46	34.16909935878431	35.0	33.0	40.0	24.0	41.0
47	34.1801999710538	35.0	33.0	40.0	24.0	41.0
48	34.02821999366244	35.0	33.0	40.0	24.0	41.0
49	34.006689823625834	35.0	33.0	39.0	24.0	41.0
50	33.83408281035713	35.0	33.0	39.0	24.0	41.0
51	33.11359247194801	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	6.0
12	12.0
13	18.0
14	43.0
15	103.0
16	314.0
17	763.0
18	1775.0
19	3223.0
20	5457.0
21	8577.0
22	12608.0
23	18258.0
24	26072.0
25	35313.0
26	44868.0
27	53684.0
28	61153.0
29	70733.0
30	82545.0
31	99041.0
32	123329.0
33	160952.0
34	282037.0
35	461610.0
36	234135.0
37	286537.0
38	400049.0
39	663165.0
40	470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.066201359706255	23.667948843012777	29.087040710214758	13.178809087066213
2	30.700759423294805	26.360933597802127	29.386544608069105	13.551762370833965
3	29.12236272392658	26.370369803631284	30.805003994447937	13.702263477994196
4	25.616780379322723	29.44736988077864	29.725578557369897	15.210271182528738
5	23.524078583191947	33.142505197882976	28.247186512346445	15.08622970657863
6	21.994839415541815	41.96070330337338	25.210640979784205	10.833816301300603
7	88.2031168807984	4.506585260263947	5.520658596160357	1.769639262777292
8	88.5376877597746	3.7923346129593534	5.064755962502558	2.605221664763486
9	83.22140590540714	5.499267737676028	7.801128136661764	3.478198220255071
10	51.32071814627012	23.367616089241004	12.804612519422326	12.507053245066555
11	44.16510937391412	22.955961609944232	18.430758970611958	14.448170045529693
12	40.34647452511338	20.234540721372433	23.016149301178825	16.402835452335367
13	23.264837955480235	35.54089543217504	24.277126063246808	16.917140549097915
14	17.49514003520725	38.87299185744698	25.588248608319038	18.043619499026732
15	16.561625118669852	23.821382824957745	43.28502378497692	16.331968271395482
16	18.15264593124194	18.646962848765035	43.416875633995076	19.783515585997947
17	18.075179782036397	20.279043908323434	27.90423143697475	33.741544872665415
18	22.303620123856575	23.39780557207954	30.74513509395082	23.553439210113062
19	29.50953407458556	25.60785423867352	23.646940533413414	21.235671153327505
20	32.887121938094666	22.755123445337272	22.933295588510017	21.42445902805805
21	25.222117446333176	27.39368807091436	24.42294094656621	22.961253536186256
22	24.543539482551626	25.969841121072257	22.89019508080389	26.596424315572225
23	23.912971403833268	30.428544012568004	21.432460675568578	24.226023908030147
24	23.363726842243853	24.269570722768734	32.49083317234401	19.875869262643402
25	21.473393406259902	24.569839718393013	31.1288635046451	22.827903370701982
26	20.544564713563336	32.438200821587486	25.163778741375914	21.853455723473264
27	21.69696135044857	30.88339463679215	25.554424911073326	21.865219101685955
28	19.498771699288522	28.430554944539978	32.630527273503965	19.44014608266754
29	19.40651365986431	25.437141798757608	31.874833830328093	23.28151071104999
30	22.030543978138606	27.472811931954755	28.084666994383547	22.411977095523095
31	28.83650306963601	26.546916114042922	22.32934653637052	22.28723427995055
32	31.34774522499294	26.355769187855095	23.067060181953	19.229425405198967
33	28.994655154495558	27.004253306019343	22.496424761879258	21.504666777605845
34	23.349795687016357	27.385495148961347	24.9579674412644	24.306741722757895
35	22.954144502740643	25.759056685456194	27.445555323900955	23.84124348790221
36	30.086768462924958	25.418333145246798	23.768846111422466	20.72605228040578
37	24.18821532656604	30.336541005733768	26.330234049783634	19.14500961791655
38	23.82954386783701	30.061647752812213	23.91182375717837	22.196984622172405
39	23.19110165790311	30.381809290454704	24.383060225308544	22.044028826333644
40	25.664758385312165	26.177852077272323	25.324768063798953	22.83262147361656
41	19.813258761804022	24.409998042624874	27.33028059323131	28.446462602339796
42	24.014761286307873	26.193568460629663	23.41208739711826	26.379582855944207
43	24.08004962934201	26.591738091731397	24.944546351216857	24.38366592770974
44	22.806384995922667	29.690543455321798	25.76712209111422	21.73594945764132
45	20.62853419381329	31.598442260940416	22.919460070503757	24.85356347474253
46	23.354035603824723	30.007835876327043	24.780050330681632	21.858078189166598
47	24.208362901174237	26.461161405663127	25.935826149384066	23.394649543778577
48	25.995790687102428	22.644949366467166	27.76029741900643	23.59896252742397
49	22.752318086847524	23.890528535915283	28.446271327897314	24.91088204933988
50	21.69186069864903	29.731316790644385	25.570842634053097	23.005979876653488
51	20.35344966644925	31.208083002906733	23.503867250436265	24.93460008020775
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2197.0
1	2990.0
2	3783.0
3	11543.0
4	19303.0
5	14270.0
6	9237.0
7	10150.0
8	11063.0
9	12035.5
10	13008.0
11	13828.0
12	14648.0
13	14801.5
14	14955.0
15	14556.0
16	14157.0
17	14220.5
18	14284.0
19	13769.5
20	13255.0
21	13233.5
22	13212.0
23	12234.5
24	11257.0
25	12632.5
26	15533.5
27	17059.0
28	19761.5
29	22464.0
30	26961.0
31	31458.0
32	37025.0
33	42592.0
34	49766.0
35	56940.0
36	60534.5
37	64129.0
38	79824.5
39	95520.0
40	131074.5
41	166629.0
42	205991.5
43	245354.0
44	255353.5
45	265353.0
46	260549.0
47	255745.0
48	254264.5
49	252784.0
50	237377.5
51	221971.0
52	211461.5
53	200952.0
54	184607.5
55	168263.0
56	167241.5
57	166220.0
58	160695.0
59	155170.0
60	146682.0
61	138194.0
62	123959.0
63	109724.0
64	96597.0
65	83470.0
66	72321.5
67	61173.0
68	54269.5
69	47366.0
70	42176.0
71	36986.0
72	30902.0
73	24818.0
74	21085.0
75	13709.0
76	10066.0
77	8108.5
78	6151.0
79	4258.5
80	2366.0
81	1701.5
82	1037.0
83	824.0
84	611.0
85	530.5
86	450.0
87	255.5
88	61.0
89	47.5
90	34.0
91	23.0
92	12.0
93	10.5
94	9.0
95	6.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3136854.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.369763269786997
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.44189489051244	14.960683293815888
2	7.216616221205493	2.6513506318489943
3	2.2509027196831233	1.2404565031169608
4	1.1025539629384917	0.810146211653824
5	0.649412283373173	0.5964774955028508
6	0.45661641082537413	0.5032761223177156
7	0.35390294153965957	0.4550779279595369
8	0.27549050606829	0.40485563036386446
9	0.23054719512244165	0.38115876572213797
>10	3.0117859545890155	13.763410363985749
>50	1.490902560105566	19.942956766980334
>100	1.5121576921167714	42.58968609084884
>500	0.005104468187506577	0.6283740788879545
>1k	0.0017601614439677852	0.5333775242016927
>5k	1.7601614439677853E-4	0.1737939011023338
>10k+	1.7601614439677853E-4	0.36491869169133756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11286	0.3597872263101821	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5375	0.17135002139085848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.187907374713646E-5	0.0	0.0	0.03675657203044834	0.0
2	3.187907374713646E-5	0.0	0.0	0.12375456428638375	0.0
3	3.187907374713646E-5	0.0	0.0	0.18559996735582848	0.0
4	3.187907374713646E-5	0.0	0.0	0.38509921086540844	0.0
5	3.187907374713646E-5	0.0	0.0	0.6222795195441038	0.0
6	3.187907374713646E-5	0.0	0.0	1.232986935317997	0.0
7	3.187907374713646E-5	0.0	0.0	1.5143516402102233	0.0
8	3.187907374713646E-5	0.0	0.0	2.3233150156175584	0.0
9	3.187907374713646E-5	0.0	0.0	2.7469879057170017	0.0
10	6.375814749427292E-5	0.0	0.0	3.356802707425975	0.0
11	6.375814749427292E-5	0.0	0.0	3.787552751897283	0.0
12	6.375814749427292E-5	0.0	0.0	4.157063095700342	0.0
13	6.375814749427292E-5	0.0	0.0	4.354490199416358	0.0
14	6.375814749427292E-5	0.0	0.0	4.440946247418592	0.0
15	6.375814749427292E-5	0.0	0.0	4.514491270553235	0.0
16	6.375814749427292E-5	0.0	0.0	4.641688774804311	0.0
17	6.375814749427292E-5	0.0	0.0	4.773636261043708	0.0
18	6.375814749427292E-5	0.0	0.0	4.975940863043036	0.0
19	6.375814749427292E-5	0.0	0.0	5.064405292691339	0.0
20	1.5939536873568232E-4	0.0	0.0	5.161955258357577	0.0
21	1.5939536873568232E-4	0.0	0.0	5.282330640826765	0.0
22	2.2315351622995524E-4	0.0	0.0	5.415712685384784	0.0
23	2.2315351622995524E-4	0.0	0.0	5.572653365441936	0.0
24	2.2315351622995524E-4	0.0	0.0	5.690478422011353	0.0
25	2.2315351622995524E-4	0.0	0.0	5.790642471724856	0.0
26	2.2315351622995524E-4	0.0	0.0	5.885259562606357	0.0
27	2.2315351622995524E-4	0.0	0.0	5.977326327588087	0.0
28	2.2315351622995524E-4	0.0	0.0	6.073186702345726	0.0
29	2.2315351622995524E-4	0.0	0.0	6.172904445026769	0.0
30	2.2315351622995524E-4	0.0	0.0	6.323278035892011	0.0
31	2.2315351622995524E-4	0.0	0.0	6.447128237399637	0.0
32	2.2315351622995524E-4	0.0	0.0	6.566897917467628	0.0
33	2.2315351622995524E-4	0.0	0.0	6.673597177299294	0.0
34	2.2315351622995524E-4	0.0	0.0	6.782145423408294	0.0
35	2.2315351622995524E-4	0.0	0.0	6.912977142066542	0.0
36	2.2315351622995524E-4	0.0	0.0	7.028730058842394	0.0
37	2.2315351622995524E-4	0.0	0.0	7.151974557948824	0.0
38	2.2315351622995524E-4	0.0	0.0	7.2687476050845845	0.0
39	2.2315351622995524E-4	0.0	0.0	7.3883578897838404	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	130	0.0	45.000004	1
TAGCGAT	20	7.0351537E-4	45.000004	25
GCTAACG	95	0.0	42.63158	1
ACGGGTA	395	0.0	42.1519	5
AGCGTTG	115	0.0	41.086956	1
TCATGCA	605	0.0	40.537193	24
TCGTTAG	185	0.0	40.135136	1
CGAATTA	45	1.9301297E-8	40.0	43
CGGGTAT	300	0.0	39.75	6
CGTTTTT	8210	0.0	39.683315	1
GCGATAA	240	0.0	39.375004	9
TAACGCC	110	0.0	38.863636	12
CGTAAGG	510	0.0	38.82353	2
CGCACAT	35	6.2517465E-6	38.571426	34
TCGACAG	35	6.2517465E-6	38.571426	1
TATGGGC	1170	0.0	38.461536	4
CGGTCTA	170	0.0	38.38235	31
TACGAAT	305	0.0	38.360657	12
TAGGGCG	1450	0.0	38.327583	5
GGGCGAT	7205	0.0	38.192226	7
>>END_MODULE