##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522442
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.455325595141097	31.0	31.0	33.0	30.0	34.0
2	31.852936968160158	31.0	31.0	34.0	30.0	34.0
3	31.966865032838015	33.0	31.0	34.0	30.0	34.0
4	35.641052145074354	37.0	35.0	37.0	33.0	37.0
5	35.51227869756266	37.0	35.0	37.0	33.0	37.0
6	35.57136838590955	37.0	35.0	37.0	33.0	37.0
7	35.986870188352285	37.0	35.0	37.0	35.0	37.0
8	36.00117106257534	37.0	35.0	37.0	35.0	37.0
9	37.738911527854825	39.0	37.0	39.0	35.0	39.0
10	37.19721403503592	39.0	37.0	39.0	33.0	39.0
11	36.881507204376966	39.0	37.0	39.0	33.0	39.0
12	35.856484790949004	37.0	35.0	39.0	32.0	39.0
13	35.371159269620335	37.0	35.0	39.0	30.0	39.0
14	36.33125627050779	38.0	35.0	41.0	31.0	41.0
15	36.65986892048187	38.0	35.0	41.0	32.0	41.0
16	36.85561124924129	38.0	35.0	41.0	32.0	41.0
17	36.79633021636694	38.0	35.0	41.0	32.0	41.0
18	36.72450021888224	38.0	35.0	40.0	32.0	41.0
19	36.65182932749496	37.0	35.0	40.0	32.0	41.0
20	36.52018486038947	37.0	35.0	40.0	32.0	41.0
21	36.36676828177725	37.0	35.0	40.0	31.0	41.0
22	36.23609615147673	37.0	35.0	40.0	31.0	41.0
23	36.1732661602377	37.0	35.0	40.0	31.0	41.0
24	36.06778138575454	36.0	35.0	40.0	31.0	41.0
25	35.935803059354846	36.0	35.0	40.0	31.0	41.0
26	35.73851180516244	36.0	34.0	40.0	30.0	41.0
27	35.673678658158174	36.0	34.0	40.0	30.0	41.0
28	35.72435742022154	36.0	35.0	40.0	30.0	41.0
29	35.777351905297515	36.0	35.0	40.0	30.0	41.0
30	35.82214724898238	36.0	35.0	40.0	30.0	41.0
31	35.644194851185624	36.0	35.0	40.0	30.0	41.0
32	35.39520338447021	36.0	34.0	40.0	30.0	41.0
33	35.27977664358988	36.0	34.0	40.0	29.0	41.0
34	35.10933948664024	36.0	34.0	40.0	29.0	41.0
35	35.00607078839055	36.0	34.0	40.0	28.0	41.0
36	34.81070916142835	36.0	34.0	40.0	27.0	41.0
37	34.51324081418516	35.0	34.0	40.0	25.0	41.0
38	34.60589329788823	35.0	34.0	40.0	26.0	41.0
39	34.515694509661195	35.0	34.0	40.0	25.0	41.0
40	34.288150663658904	35.0	33.0	40.0	24.0	41.0
41	34.257730574414	35.0	34.0	40.0	24.0	41.0
42	34.22291978121996	35.0	34.0	40.0	24.0	41.0
43	34.11841529257259	35.0	33.0	40.0	24.0	41.0
44	33.97771801494531	35.0	33.0	40.0	23.0	41.0
45	33.929875921306866	35.0	33.0	40.0	23.0	41.0
46	33.760808552702926	35.0	33.0	39.0	23.0	41.0
47	33.7778072712056	35.0	33.0	39.0	23.0	41.0
48	33.64121822303959	35.0	33.0	39.0	23.0	41.0
49	33.66812086614911	35.0	33.0	39.0	24.0	41.0
50	33.500400574374254	35.0	33.0	39.0	23.0	41.0
51	32.793782835884876	35.0	32.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	13.0
13	13.0
14	48.0
15	118.0
16	302.0
17	980.0
18	2082.0
19	3984.0
20	6849.0
21	10875.0
22	16222.0
23	23147.0
24	33139.0
25	44168.0
26	55804.0
27	64101.0
28	71893.0
29	82345.0
30	96727.0
31	116399.0
32	143734.0
33	190006.0
34	347883.0
35	619660.0
36	241716.0
37	277544.0
38	392377.0
39	679779.0
40	531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.940398166953493	24.91652098175073	29.679012457834652	13.464068393461126
2	30.284671827101768	27.59991505892787	28.793859487253442	13.32155362671692
3	28.622983714139227	26.060443294737002	31.36432054807432	13.952252443049455
4	25.261111467555747	29.565994273291086	30.18715425264632	14.98574000650685
5	23.102097919568298	33.73151353521222	29.012486224045702	14.153902321173776
6	21.195948719666642	41.40411680305879	26.200573352236884	11.199361125037687
7	85.67570452544003	5.575648939003112	6.699698674953342	2.0489478606035245
8	86.00933670447944	4.541281304276977	6.126261269880384	3.323120721363191
9	80.83602228226894	6.425258386085562	9.13045551921082	3.6082638124346684
10	53.41226342406773	21.146522781638417	12.846457088576619	12.594756705717227
11	48.53073521153791	20.803465323204755	17.89179211467499	12.77400735058235
12	42.8395982105596	21.137409785597605	21.757434189122204	14.265557814720584
13	21.52222804520273	41.69573267636486	22.891562160569286	13.890477117863117
14	15.450162131839217	44.48144213588186	25.19732049527004	14.87107523700887
15	13.6034603266711	24.05064441089449	48.6205024809493	13.725392781485118
16	15.095663746911944	18.810359404072514	47.77813232978712	18.31584451922842
17	15.635459718002453	20.31479865388841	27.81240968623472	36.237331941874416
18	21.367392280696176	23.68359791303874	32.81155516542217	22.137454640842915
19	27.979566448503622	26.260645313677273	24.495846915293424	21.263941322525678
20	30.966812228561892	24.052745226181155	23.672469269898553	21.307973275358403
21	23.1288123409839	29.72239145456476	25.843832205044116	21.304963999407228
22	23.52333977394092	26.48639778880674	23.538897162820565	26.451365274431772
23	20.28655120510146	32.485502955052205	21.73284897238904	25.49509686745729
24	20.833870366069903	25.27771926407873	35.72799779244058	18.160412577410785
25	18.043703771417672	26.008462311089865	33.60691247719622	22.34092144029625
26	16.980208616635846	35.57778949944385	26.562452980063263	20.87954890385704
27	19.424847875422792	33.16117057427773	27.54793407528073	19.86604747501875
28	15.771302976741703	30.195074894064973	36.4030124555635	17.63060967362983
29	16.074104271979497	25.826599841814286	35.75516644418843	22.34412944201778
30	18.672074657297408	29.363123651148832	31.203494621061186	20.761307070492574
31	27.77098955781245	26.788716464316515	24.211867789448345	21.22842618842269
32	28.688875501711596	27.164450117276594	25.27419897900377	18.87247540200804
33	25.947197995027317	27.83776141665356	25.56913641161444	20.645904176704683
34	19.214539231589903	27.890395356403314	28.573245492757582	24.3218199192492
35	20.025482321639362	25.365385718203452	30.544519966545934	24.06461199361125
36	29.68957331305952	24.13723206798011	25.57322448460471	20.599970134355654
37	20.827936982354856	29.65695389732464	29.28712523868385	20.22798388163666
38	21.814269759445292	30.65827627537941	23.972715519517426	23.55473844565787
39	20.81453718755341	30.16960960606307	26.770320135860292	22.245533070523233
40	24.273444388864316	25.680138949058634	24.949083618694075	25.097333043382967
41	17.48508563093445	23.93725716420597	27.58322209421759	30.994435110641994
42	23.45063453138476	25.884088368239986	24.30308291804379	26.36219418233146
43	22.83591894486836	26.412471802232655	26.15126097179173	24.600348281107255
44	21.217240766490974	29.923445155378005	26.586186514923455	22.27312756320757
45	18.0428520895447	33.80671704459577	23.337616346841198	24.81281451901834
46	23.111949039899024	30.21895037590399	26.156882072153355	20.512218512043635
47	23.575633040941483	25.869609776399443	27.36408434830155	23.190672834357528
48	24.670299752274133	21.758654933140136	29.8372549498331	23.733790364752636
49	21.491482329588393	21.866052017322072	30.836533291392733	25.805932361696797
50	19.551776863891586	30.295147514139337	27.40050794306904	22.752567678900036
51	18.925222899340856	31.72120364224592	23.88008092113369	25.473492537279537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3881.0
1	4513.5
2	5146.0
3	14587.0
4	24028.0
5	17837.5
6	11647.0
7	13239.5
8	14832.0
9	17032.5
10	19233.0
11	20492.5
12	21752.0
13	22599.5
14	23447.0
15	22762.0
16	22077.0
17	21844.5
18	21612.0
19	21616.5
20	21621.0
21	20801.0
22	19981.0
23	19658.5
24	19336.0
25	19456.5
26	20949.5
27	22322.0
28	24792.5
29	27263.0
30	31121.0
31	34979.0
32	41446.0
33	47913.0
34	50446.0
35	52979.0
36	60666.0
37	68353.0
38	89015.0
39	109677.0
40	163813.0
41	217949.0
42	267796.5
43	317644.0
44	332409.5
45	347175.0
46	348280.5
47	349386.0
48	341312.0
49	333238.0
50	310072.0
51	286906.0
52	252606.5
53	218307.0
54	196895.5
55	175484.0
56	161134.5
57	146785.0
58	133307.5
59	119830.0
60	115088.0
61	110346.0
62	98831.5
63	87317.0
64	75967.5
65	64618.0
66	54633.5
67	44649.0
68	37740.0
69	30831.0
70	26768.0
71	22705.0
72	18930.5
73	15156.0
74	12372.0
75	8238.0
76	6888.0
77	5040.5
78	3193.0
79	2214.5
80	1236.0
81	1143.5
82	1051.0
83	706.0
84	361.0
85	218.5
86	76.0
87	54.5
88	33.0
89	28.0
90	23.0
91	14.0
92	5.0
93	4.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3522442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.25957635057989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.78400144435747	14.750816422786015
2	8.623129429324232	3.1490938039135634
3	2.854484024051725	1.5636500693604898
4	1.3280652987376504	0.9699963888342328
5	0.7742322447862658	0.7068576393377841
6	0.5282929484502927	0.5787843256620652
7	0.39134805140177575	0.5002094736954972
8	0.290784654237447	0.42476836765005094
9	0.23185005255450492	0.3810135362854448
>10	1.9790261032261982	8.026043283872628
>50	0.7026353274185613	9.406999076152283
>100	1.4908547923474207	55.399602282241425
>500	0.017194248685937774	2.0484862080364574
>1k	0.0037858896189220783	1.3430850179914222
>5k	0.0	0.0
>10k+	3.154908015768398E-4	0.7505941041806273
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14348	0.4073310504473885	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11711	0.3324682138130309	No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	4220	0.11980325013158485	No Hit
GAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	3849	0.10927078430248106	TruSeq Adapter, Index 23 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.67787915315568E-5	0.0	0.0	0.059419005337774194	0.0
2	5.67787915315568E-5	0.0	0.0	0.28346811672129735	0.0
3	5.67787915315568E-5	0.0	0.0	0.4371399160014558	0.0
4	5.67787915315568E-5	0.0	0.0	0.8188637314681122	0.0
5	5.67787915315568E-5	0.0	0.0	1.3246208170354543	0.0
6	5.67787915315568E-5	0.0	0.0	2.3268232663589634	0.0
7	5.67787915315568E-5	0.0	0.0	2.765751714293663	0.0
8	5.67787915315568E-5	0.0	0.0	3.9018953328401147	0.0
9	5.67787915315568E-5	0.0	0.0	4.472011178608477	0.0
10	5.67787915315568E-5	0.0	0.0	5.358413282603376	0.0
11	5.67787915315568E-5	0.0	0.0	6.094039305686225	0.0
12	5.67787915315568E-5	0.0	0.0	6.705348164710732	0.0
13	5.67787915315568E-5	0.0	0.0	7.015530702847626	0.0
14	5.67787915315568E-5	0.0	0.0	7.147513003762731	0.0
15	5.67787915315568E-5	0.0	0.0	7.283413041293512	0.0
16	5.67787915315568E-5	0.0	0.0	7.521827186934519	0.0
17	8.51681872973352E-5	0.0	0.0	7.756351985355614	0.0
18	8.51681872973352E-5	0.0	0.0	8.139637217589389	0.0
19	8.51681872973352E-5	0.0	0.0	8.28266299345738	0.0
20	2.271151661262272E-4	0.0	0.0	8.445135505424929	0.0
21	2.271151661262272E-4	0.0	0.0	8.638240175423753	0.0
22	2.271151661262272E-4	0.0	0.0	8.826717373912757	0.0
23	2.271151661262272E-4	0.0	0.0	9.040517913424834	0.0
24	2.271151661262272E-4	0.0	0.0	9.206737825633466	0.0
25	2.271151661262272E-4	0.0	0.0	9.341161614584427	0.0
26	2.271151661262272E-4	0.0	0.0	9.475812518701515	0.0
27	2.271151661262272E-4	0.0	0.0	9.603394463272922	0.0
28	2.271151661262272E-4	0.0	0.0	9.730124725971358	0.0
29	2.555045618920056E-4	0.0	0.0	9.863214213321326	0.0
30	2.555045618920056E-4	0.0	0.0	10.071251705492951	0.0
31	2.555045618920056E-4	0.0	0.0	10.238493635949151	0.0
32	2.555045618920056E-4	0.0	0.0	10.374507231062996	0.0
33	2.555045618920056E-4	0.0	0.0	10.502060786238637	0.0
34	2.83893957657784E-4	0.0	0.0	10.631147368785632	0.0
35	2.83893957657784E-4	0.0	0.0	10.796742714287417	0.0
36	2.83893957657784E-4	0.0	0.0	10.943828173749916	0.0
37	3.406727491893408E-4	0.0	0.0	11.088330198197728	0.0
38	3.406727491893408E-4	0.0	0.0	11.22207264165031	0.0
39	3.406727491893408E-4	0.0	0.0	11.367369569179564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAT	60	0.0	45.000004	15
GTACGAT	30	2.1667838E-6	45.000004	39
CGTATAA	25	3.8924532E-5	45.0	26
CGTCGAA	25	3.8924532E-5	45.0	25
GCTCGAC	25	3.8924532E-5	45.0	13
TCGACCC	25	3.8924532E-5	45.0	15
ACGCGTA	25	3.8924532E-5	45.0	23
TCGATAT	110	0.0	42.954544	16
TTACGAT	80	0.0	42.1875	13
AACTCCG	75	0.0	42.0	1
CATTACG	70	0.0	41.785717	20
CATACGA	470	0.0	41.648937	18
TACGAAT	260	0.0	41.538464	12
GCGATAA	305	0.0	41.311478	9
GGACTAA	1095	0.0	41.30137	8
GCTACGA	265	0.0	40.75472	10
ACGGGAT	630	0.0	40.714287	5
CGCCTAG	50	1.0822987E-9	40.5	45
AATACGG	100	0.0	40.5	2
TACGGGA	625	0.0	40.32	4
>>END_MODULE