##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549292_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1739447
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.379371719862693	31.0	31.0	33.0	30.0	34.0
2	31.774293784173935	31.0	31.0	34.0	30.0	34.0
3	31.91451593523689	31.0	31.0	34.0	30.0	34.0
4	35.607015333034006	37.0	35.0	37.0	33.0	37.0
5	35.47922644380657	37.0	35.0	37.0	33.0	37.0
6	35.52942170701378	37.0	35.0	37.0	33.0	37.0
7	35.9507964312796	37.0	35.0	37.0	35.0	37.0
8	35.9609824271737	37.0	35.0	37.0	35.0	37.0
9	37.68484409125429	39.0	37.0	39.0	35.0	39.0
10	37.09705383377591	39.0	37.0	39.0	33.0	39.0
11	36.782936186040736	39.0	35.0	39.0	32.0	39.0
12	35.93785956111339	37.0	35.0	39.0	32.0	39.0
13	35.53896554479671	37.0	35.0	39.0	30.0	39.0
14	36.521382370374035	38.0	35.0	41.0	31.0	41.0
15	36.81713958516701	38.0	35.0	41.0	32.0	41.0
16	36.977494571550615	38.0	35.0	41.0	32.0	41.0
17	36.913121814001805	38.0	35.0	41.0	32.0	41.0
18	36.84491507933269	38.0	35.0	40.0	32.0	41.0
19	36.786554577403045	38.0	35.0	40.0	32.0	41.0
20	36.64841412241937	38.0	35.0	40.0	32.0	41.0
21	36.504157930652674	38.0	35.0	40.0	31.0	41.0
22	36.37319216969531	38.0	35.0	40.0	31.0	41.0
23	36.295139202286705	38.0	35.0	40.0	31.0	41.0
24	36.165006464698266	37.0	35.0	40.0	31.0	41.0
25	36.033037511347	37.0	35.0	40.0	30.0	41.0
26	35.85777203904459	37.0	35.0	40.0	30.0	41.0
27	35.79003901814772	37.0	34.0	40.0	30.0	41.0
28	35.852402516431944	37.0	35.0	40.0	30.0	41.0
29	35.87740586519739	37.0	35.0	40.0	30.0	41.0
30	35.89642282863462	37.0	35.0	40.0	30.0	41.0
31	35.75582412111435	37.0	35.0	40.0	30.0	41.0
32	35.507498072663324	37.0	34.0	40.0	29.0	41.0
33	35.40356274149198	37.0	34.0	40.0	29.0	41.0
34	35.2571857607619	37.0	34.0	40.0	28.0	41.0
35	35.16722153649982	36.0	34.0	40.0	28.0	41.0
36	34.96260823123671	36.0	34.0	40.0	26.0	41.0
37	34.66846014854146	36.0	34.0	40.0	25.0	41.0
38	34.769594014649485	36.0	34.0	40.0	26.0	41.0
39	34.66905459033819	36.0	34.0	40.0	25.0	41.0
40	34.488190212176626	36.0	34.0	40.0	24.0	41.0
41	34.442053710173404	36.0	34.0	40.0	24.0	41.0
42	34.396930173785115	36.0	34.0	40.0	24.0	41.0
43	34.3055407839388	35.0	33.0	40.0	24.0	41.0
44	34.146939228386955	35.0	33.0	40.0	23.0	41.0
45	34.071687151146314	35.0	33.0	40.0	23.0	41.0
46	33.89900123430033	35.0	33.0	40.0	23.0	41.0
47	33.92065006867125	35.0	33.0	40.0	23.0	41.0
48	33.759272343451684	35.0	33.0	39.0	23.0	41.0
49	33.7662872165694	35.0	33.0	39.0	24.0	41.0
50	33.586346695242796	35.0	33.0	39.0	23.0	41.0
51	32.88032978297125	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	4.0
13	7.0
14	15.0
15	70.0
16	198.0
17	556.0
18	1139.0
19	2229.0
20	3550.0
21	5536.0
22	8278.0
23	11775.0
24	16654.0
25	22051.0
26	27412.0
27	31538.0
28	35123.0
29	40649.0
30	47778.0
31	57620.0
32	70311.0
33	91108.0
34	159611.0
35	266147.0
36	124496.0
37	149301.0
38	212996.0
39	353041.0
40	251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.65066426283756	24.81978467869386	29.26079380400782	13.268757254460755
2	29.708982222510947	27.55364204830616	29.17381213684579	13.563563592337106
3	28.8064540052097	26.33543879175393	30.99755267047516	13.860554532561212
4	24.71072702991238	29.563936124526936	30.546719733340538	15.178617112220147
5	23.77859170184547	33.22377744191113	28.829909735680364	14.167721120563028
6	21.780255449001896	42.210829073837836	25.33834028860897	10.670575188551304
7	87.37535550091494	4.989804230884873	5.994146415498719	1.6406938527014618
8	87.54253507005387	4.415886198314752	5.26029249525855	2.781286236372824
9	82.28436968760762	6.4061739161929045	8.218014115980539	3.091442280218943
10	50.15461810563933	25.602217256403904	12.31052167729169	11.932642960665085
11	44.73122779826002	22.531298740346788	19.319875799607576	13.417597661785614
12	39.835763895077	22.297603778672187	22.851975369183425	15.014656957067391
13	22.34169825237561	39.820759126320034	23.102744722891817	14.734797898412541
14	16.721003859272514	41.57988142208415	26.03212400262842	15.666990716014917
15	15.379600528213851	24.79178727492128	44.91438945825886	14.914222738606005
16	16.6417257898631	19.66400815891487	44.27614063550082	19.418125415721203
17	16.9920095294654	21.262332223976934	28.038393811366486	33.707264435191185
18	21.94300832390984	24.1314624705438	31.88990523999869	22.03562396554767
19	28.889066467676223	27.07998576559102	23.511725278206235	20.519222488526527
20	31.476210542775952	24.534349135098683	22.828864575925568	21.160575746199797
21	23.350294662614036	29.843277777362577	24.709519749667567	22.09690781035582
22	23.226117266004657	27.76802052606374	22.513821921564727	26.492040286366876
23	21.28728268236974	32.111757357367026	22.17865792979033	24.422302030472903
24	21.131658509859744	25.696615073641222	34.651587544777165	18.520138871721876
25	19.177819157467862	27.292869515426453	31.534447442204332	21.99486388490135
26	18.978847875215514	35.01256433797638	25.13862164239554	20.86996614441256
27	20.453971865771134	32.92454440980381	26.624783623760884	19.996700100664178
28	17.519533506913405	30.199080512369736	34.229556864911665	18.051829115805194
29	17.58455417152693	26.364183559487586	32.94409085186269	23.1071714171228
30	19.756566311017238	30.312162428633926	28.973863532490498	20.95740772785834
31	27.674542541393905	28.255244339149165	22.307376999701628	21.762836119755303
32	28.715850497313227	27.89719951225878	23.114127650914345	20.27282233951365
33	25.57128788632249	30.032015922301742	22.29369449026041	22.103001701115353
34	19.71132204660447	28.31491847696423	26.68537759414343	25.288381882287876
35	20.831275687043068	27.91674595431767	27.633092586321972	23.618885772317295
36	27.839480018649603	26.931662764085367	24.10426992026776	21.124587296997262
37	21.471134216794187	32.512574398645086	26.10151387193746	19.914777512623264
38	21.141661689031054	32.24208613427141	23.56726016946765	23.048992007229884
39	21.58444609120025	32.7499486905896	23.776177141355845	21.889428076854312
40	24.49899307078629	27.053310621134187	24.22344572729149	24.22425058078803
41	18.570442215255767	26.047818645810995	25.415318776599687	29.966420362333544
42	22.74320516807928	27.950607290707907	22.880087752026938	26.426099789185876
43	23.10906857179322	27.56128815652331	24.82858057762036	24.501062694063116
44	21.781060302498435	31.235904284522608	25.664880850063266	21.318154562915687
45	18.87387198345221	34.739948960790414	22.479328200284343	23.90685085547303
46	22.28708319368167	32.230415758571546	24.51411281861419	20.968388229132593
47	23.092109158830365	28.363094707685832	25.68402486537388	22.86077126810992
48	24.11329577733613	24.52009173030279	28.075129624530092	23.291482867830986
49	21.648316965104428	25.14253093080732	28.77489225023815	24.4342598538501
50	19.183740579620995	32.03615861822752	26.59442914903415	22.185671653117343
51	19.12814819882411	33.44057048015835	23.50586134558857	23.925419975428973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1678.0
1	1895.5
2	2113.0
3	6507.0
4	10901.0
5	8174.5
6	5448.0
7	6090.0
8	6732.0
9	7734.5
10	8737.0
11	9464.5
12	10192.0
13	10326.5
14	10461.0
15	10290.5
16	10120.0
17	10006.5
18	9893.0
19	9692.0
20	9491.0
21	9504.0
22	9517.0
23	9391.0
24	9265.0
25	9341.0
26	10496.5
27	11576.0
28	13259.0
29	14942.0
30	18007.0
31	21072.0
32	24725.0
33	28378.0
34	31933.5
35	35489.0
36	37943.5
37	40398.0
38	50760.0
39	61122.0
40	82549.5
41	103977.0
42	125107.5
43	146238.0
44	155171.5
45	164105.0
46	153709.0
47	143313.0
48	143555.5
49	143798.0
50	145039.0
51	146280.0
52	136974.5
53	127669.0
54	112266.5
55	96864.0
56	85042.5
57	73221.0
58	67476.5
59	61732.0
60	60404.5
61	59077.0
62	51307.5
63	43538.0
64	36982.0
65	30426.0
66	26740.5
67	23055.0
68	19794.0
69	16533.0
70	14532.0
71	12531.0
72	10108.0
73	7685.0
74	6743.0
75	4642.5
76	3484.0
77	2486.5
78	1489.0
79	1240.5
80	992.0
81	709.0
82	426.0
83	284.5
84	143.0
85	100.5
86	58.0
87	49.0
88	40.0
89	30.5
90	21.0
91	13.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1739447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.832465989521474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.45357927621059	18.59786078548869
2	6.74513298917622	3.0801603914033064
3	2.3081820363221865	1.5810446352585221
4	1.1709310820971	1.0694097643222245
5	0.7846443756163288	0.895768301006457
6	0.5615310833930741	0.7692683618178908
7	0.45820598060634443	0.7323380727872787
8	0.39131647556877763	0.7147776095650821
9	0.31739249598427244	0.6522168032901208
>10	3.561218120922044	19.228327609461445
>50	1.322040424505733	22.042273662126608
>100	0.9202453405560554	29.107315093092907
>500	0.003804762982673653	0.543529906226541
>1k	0.001521905193069461	0.5937999071433909
>5k	2.5365086551157686E-4	0.39190909700959004
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6767	0.3890316865072635	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3904	0.22443914646436483	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.748953546730656E-5	0.0	0.0	0.03340142010650511	0.0
2	5.748953546730656E-5	0.0	0.0	0.11198961509031319	0.0
3	5.748953546730656E-5	0.0	0.0	0.1821843378958945	0.0
4	5.748953546730656E-5	0.0	0.0	0.40150691570366903	0.0
5	5.748953546730656E-5	0.0	0.0	0.6710753475098695	0.0
6	5.748953546730656E-5	0.0	0.0	1.4019398119057378	0.0
7	5.748953546730656E-5	0.0	0.0	1.7516486561533637	0.0
8	5.748953546730656E-5	0.0	0.0	2.8141127611246564	0.0
9	5.748953546730656E-5	0.0	0.0	3.356239080581357	0.0
10	1.1497907093461312E-4	0.0	0.0	4.087276013583628	0.0
11	1.1497907093461312E-4	0.0	0.0	4.562082087008112	0.0
12	1.1497907093461312E-4	0.0	0.0	5.003601719397027	0.0
13	1.1497907093461312E-4	0.0	0.0	5.258855256871868	0.0
14	1.1497907093461312E-4	0.0	0.0	5.383780017442325	0.0
15	1.1497907093461312E-4	0.0	0.0	5.475475826512679	0.0
16	1.1497907093461312E-4	0.0	0.0	5.622246610560713	0.0
17	1.1497907093461312E-4	0.0	0.0	5.7774108667869735	0.0
18	1.1497907093461312E-4	0.0	0.0	6.0223162878776995	0.0
19	1.1497907093461312E-4	0.0	0.0	6.1328111750458625	0.0
20	1.724686064019197E-4	0.0	0.0	6.256816103048843	0.0
21	1.724686064019197E-4	0.0	0.0	6.398067891692015	0.0
22	2.2995814186922624E-4	0.0	0.0	6.559843444497015	0.0
23	2.2995814186922624E-4	0.0	0.0	6.72943757412557	0.0
24	2.2995814186922624E-4	0.0	0.0	6.8728739651165	0.0
25	2.2995814186922624E-4	0.0	0.0	6.991992282604759	0.0
26	2.2995814186922624E-4	0.0	0.0	7.109040976816195	0.0
27	2.2995814186922624E-4	0.0	0.0	7.221318039583845	0.0
28	2.2995814186922624E-4	0.0	0.0	7.3329627174613545	0.0
29	2.2995814186922624E-4	0.0	0.0	7.460531996663307	0.0
30	2.2995814186922624E-4	0.0	0.0	7.65898587309645	0.0
31	2.2995814186922624E-4	0.0	0.0	7.79943280824308	0.0
32	2.2995814186922624E-4	0.0	0.0	7.94413396901429	0.0
33	2.2995814186922624E-4	0.0	0.0	8.067104085378858	0.0
34	2.2995814186922624E-4	0.0	0.0	8.201687087907823	0.0
35	2.2995814186922624E-4	0.0	0.0	8.35650640692128	0.0
36	2.2995814186922624E-4	0.0	0.0	8.49522865600389	0.0
37	2.2995814186922624E-4	0.0	0.0	8.641079607484448	0.0
38	2.2995814186922624E-4	0.0	0.0	8.784515998475378	0.0
39	2.2995814186922624E-4	0.0	0.0	8.938013058173086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.034286E-4	45.000004	45
ACGTTAG	40	6.8193913E-9	45.000004	1
CGTAGGT	20	7.034286E-4	45.000004	14
CTACGGT	20	7.034286E-4	45.000004	35
GTCCGAT	20	7.034286E-4	45.000004	38
TACTCGT	20	7.034286E-4	45.000004	11
CTCGATA	20	7.034286E-4	45.000004	24
TGTACCG	20	7.034286E-4	45.000004	34
GTTGCGA	20	7.034286E-4	45.000004	23
TCGCACA	35	1.212411E-7	45.0	25
TCCGCAT	25	3.8916347E-5	45.0	30
CTCCGAT	25	3.8916347E-5	45.0	12
GCACGAC	25	3.8916347E-5	45.0	39
TTATCCG	35	1.212411E-7	45.0	1
CGTTACC	25	3.8916347E-5	45.0	38
TTGATCG	30	2.1661472E-6	44.999996	20
ATAGCGC	65	0.0	44.999996	12
TATTCCG	30	2.1661472E-6	44.999996	1
TCGGTTA	30	2.1661472E-6	44.999996	35
TATCCGA	30	2.1661472E-6	44.999996	42
>>END_MODULE