##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549291_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1068825
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.463984281804784	31.0	31.0	33.0	30.0	34.0
2	31.8783916918111	31.0	31.0	34.0	30.0	34.0
3	32.02827123242813	33.0	31.0	34.0	30.0	34.0
4	35.71044371155241	37.0	35.0	37.0	33.0	37.0
5	35.59976048464435	37.0	35.0	37.0	33.0	37.0
6	35.640047715949756	37.0	35.0	37.0	33.0	37.0
7	36.00708160830819	37.0	35.0	37.0	35.0	37.0
8	36.021155942273055	37.0	35.0	37.0	35.0	37.0
9	37.768971534161345	39.0	38.0	39.0	35.0	39.0
10	37.178077795710244	39.0	37.0	39.0	33.0	39.0
11	36.92113208429818	39.0	37.0	39.0	33.0	39.0
12	36.27965569667626	38.0	35.0	39.0	32.0	39.0
13	35.99476247280892	38.0	35.0	39.0	31.0	39.0
14	37.049977311533695	39.0	35.0	41.0	32.0	41.0
15	37.260111805019534	39.0	35.0	41.0	32.0	41.0
16	37.36916239796038	39.0	35.0	41.0	33.0	41.0
17	37.28671391481299	39.0	35.0	41.0	32.0	41.0
18	37.21973101302832	39.0	35.0	41.0	32.0	41.0
19	37.17888335321498	39.0	35.0	41.0	32.0	41.0
20	37.07062896171029	39.0	35.0	41.0	32.0	41.0
21	36.94636914368582	38.0	35.0	41.0	32.0	41.0
22	36.84442448483147	38.0	35.0	41.0	32.0	41.0
23	36.756656141089515	38.0	35.0	41.0	31.0	41.0
24	36.61292260192267	38.0	35.0	40.0	31.0	41.0
25	36.48512057633383	38.0	35.0	40.0	31.0	41.0
26	36.29357752672327	38.0	35.0	40.0	31.0	41.0
27	36.2822987860501	38.0	35.0	40.0	31.0	41.0
28	36.316412883306434	38.0	35.0	40.0	31.0	41.0
29	36.3349444483428	38.0	35.0	40.0	31.0	41.0
30	36.37561902088742	38.0	35.0	40.0	31.0	41.0
31	36.25531962669287	38.0	35.0	40.0	31.0	41.0
32	36.064836619652425	38.0	35.0	40.0	30.0	41.0
33	35.98311697424742	38.0	35.0	40.0	30.0	41.0
34	35.82116436273478	38.0	35.0	40.0	30.0	41.0
35	35.72663532383692	38.0	35.0	40.0	29.0	41.0
36	35.55619862933595	38.0	35.0	40.0	29.0	41.0
37	35.27561013262227	37.0	34.0	40.0	27.0	41.0
38	35.343490281383765	37.0	34.0	40.0	28.0	41.0
39	35.244663064580266	37.0	34.0	40.0	27.0	41.0
40	35.078964283208194	37.0	34.0	40.0	27.0	41.0
41	35.03575234486469	37.0	34.0	40.0	26.0	41.0
42	35.007055411316166	37.0	34.0	40.0	27.0	41.0
43	34.92727808574837	37.0	34.0	40.0	27.0	41.0
44	34.778502561223775	36.0	34.0	40.0	26.0	41.0
45	34.707414216546205	36.0	34.0	40.0	26.0	41.0
46	34.53742006408907	36.0	34.0	40.0	25.0	41.0
47	34.54143101068931	36.0	34.0	40.0	26.0	41.0
48	34.3860042570112	35.0	34.0	40.0	25.0	41.0
49	34.37430262203822	36.0	34.0	40.0	25.0	41.0
50	34.222086871096764	35.0	34.0	40.0	24.0	41.0
51	33.53430636446565	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	17.0
15	31.0
16	87.0
17	217.0
18	515.0
19	965.0
20	1685.0
21	2617.0
22	3961.0
23	5666.0
24	8277.0
25	10965.0
26	14192.0
27	16779.0
28	19164.0
29	22088.0
30	26307.0
31	32196.0
32	39763.0
33	51789.0
34	88928.0
35	144008.0
36	80948.0
37	98345.0
38	144496.0
39	254629.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13388534137955	24.6480013098496	29.33913409585292	13.878979252917926
2	29.660889294318526	26.569644235492245	29.553200944962928	14.216265525226298
3	28.58400580076252	26.37269899188361	30.922087338900194	14.121207868453675
4	24.726779407293055	30.16705260449559	30.393375903445374	14.712792084765983
5	23.49177835473534	33.22031202488714	28.7041377213295	14.58377189904802
6	21.666596496152316	41.17259607512923	26.00622178560569	11.154585643112764
7	88.14436413818913	4.624611138399644	5.550955488503731	1.6800692349074917
8	89.16342712792084	3.714452786938928	4.90819357705892	2.213926508081304
9	83.66842092952541	5.397703085163615	7.5363132411760585	3.3975627441349148
10	45.883750847893715	24.738942296447032	14.42181835192852	14.955488503730733
11	40.2716066708769	25.754075737375153	19.83093584075971	14.143381750988235
12	35.503567001146116	23.22241714031764	23.68778799148598	17.586227867050265
13	21.972633499403553	36.05117769513251	25.333052651275935	16.64313615418801
14	17.976937290950342	37.591654386826654	27.63763946389727	16.79376885832573
15	17.23640446284471	24.050616331017707	41.63291464926438	17.0800645568732
16	18.62746473931654	21.465347460996888	40.70816083081889	19.199026968867685
17	18.464014221224243	22.49807031085538	28.515425818071243	30.52248964984913
18	23.116787126049633	23.932636306224126	30.73421748181414	22.2163590859121
19	28.085140224077843	26.34650199985966	24.496058756110685	21.072299019951814
20	30.6484223329357	24.89546932379014	23.980632938039438	20.475475405234718
21	24.777302177624964	28.045844736041914	25.596332421116646	21.580520665216476
22	24.759151404579796	25.01634972984352	24.57184291160854	25.652655953968146
23	22.78838911889224	30.539517694664703	23.563352279372207	23.10874090707085
24	21.7834537927163	26.122517718054873	33.062755830000235	19.03127265922859
25	20.905480317170724	26.713914812995583	30.361003906158636	22.019600963675064
26	19.510303370523708	33.10626154889715	25.608308188899027	21.775126891680117
27	21.30891399433958	30.546254064042287	26.731317100554346	21.413514841063787
28	18.56244006268566	29.50408158491802	33.14611840104788	18.787359951348442
29	19.124178420227818	25.77952424391271	32.32699459687039	22.76930273898908
30	21.09147896054078	27.881645732463216	29.663415432835123	21.363459874160878
31	27.14653942413398	27.091712862255278	23.905503707342174	21.856244006268565
32	27.597034126260144	26.817673613547587	25.874581900685335	19.710710359506937
33	25.257736299207075	26.997965055083856	24.541248567351996	23.203050078357073
34	21.039927022665076	27.70715505344654	28.195448272635836	23.057469651252543
35	21.52410357167918	26.34537927163006	28.73735176478844	23.393165391902322
36	28.311931326456623	25.914158070778658	25.307884826795778	20.466025775968937
37	22.28559399340397	29.399480738193812	27.419923748041075	20.895001520361145
38	22.9734521554043	29.710663579164038	24.905480317170724	22.41040394826094
39	22.358290646270436	29.348957967861907	26.377938390288403	21.914812995579258
40	24.484457231071506	26.573854466353236	26.265010642528008	22.676677660047247
41	19.111921970388043	25.84305194957079	27.85432601220967	27.190700067831497
42	23.81671461651814	27.217177741912845	24.601033845578087	24.365073795990924
43	23.64428227258906	26.738614834046732	25.65845671648773	23.958646176876478
44	22.639814749842117	29.604004397352234	26.315346291488318	21.440834561317335
45	19.90288400813978	31.79426005192618	23.91242719809136	24.39042874184268
46	23.190512946459897	30.207564381446915	25.69550674806446	20.906415924028725
47	23.944799195378103	26.85827895118471	27.020232498304214	22.17668935513297
48	24.668116857296564	24.40081397796646	28.28536009168947	22.645709073047506
49	22.116997637592682	25.386943606296636	28.371155240567912	24.12490351554277
50	20.812574556171498	29.81414169765864	27.19883984749608	22.174443898673776
51	21.064252800973033	30.20026664795453	24.381259794634293	24.354220756438146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	929.0
1	1091.5
2	1254.0
3	4209.5
4	7165.0
5	5221.0
6	3277.0
7	3580.0
8	3883.0
9	4227.0
10	4571.0
11	4820.5
12	5070.0
13	5157.5
14	5245.0
15	5189.0
16	5133.0
17	5001.5
18	4870.0
19	4735.5
20	4601.0
21	4664.0
22	4727.0
23	4878.5
24	5030.0
25	6217.0
26	7681.5
27	7959.0
28	10099.5
29	12240.0
30	13495.0
31	14750.0
32	16709.5
33	18669.0
34	21628.0
35	24587.0
36	25914.0
37	27241.0
38	33219.0
39	39197.0
40	49808.0
41	60419.0
42	73530.0
43	86641.0
44	87172.0
45	87703.0
46	87396.5
47	87090.0
48	86283.0
49	85476.0
50	85211.5
51	84947.0
52	78931.5
53	72916.0
54	66764.5
55	60613.0
56	55691.5
57	50770.0
58	46323.5
59	41877.0
60	40394.5
61	38912.0
62	34427.0
63	29942.0
64	25949.0
65	21956.0
66	18770.5
67	15585.0
68	13328.5
69	11072.0
70	9837.5
71	8603.0
72	7325.0
73	6047.0
74	5277.5
75	3875.5
76	3243.0
77	2288.0
78	1333.0
79	1051.0
80	769.0
81	578.5
82	388.0
83	233.0
84	78.0
85	66.5
86	55.0
87	47.5
88	40.0
89	35.5
90	31.0
91	19.5
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1068825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.4908541430967
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02190959187261	17.772702427397814
2	5.687732785946912	2.5584393698728207
3	2.1041580259249537	1.419729337653133
4	1.2095224431143128	1.0881277140354397
5	0.8644661777929469	0.9721291358190736
6	0.6931555185966726	0.935379580034419
7	0.5650290050617353	0.8895589457623776
8	0.4742247130234558	0.8532575081329948
9	0.42811400751451567	0.866578472966302
>10	6.924088801470695	39.42063772586452
>50	1.8841446325550986	27.99695830568866
>100	0.14094489251163173	4.38067419460381
>500	0.0016729364096336112	0.23111628472958626
>1k	8.364682048168056E-4	0.6147109974390909
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4340	0.40605337637124883	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2195	0.20536570533062007	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.356068579982691E-5	0.0	0.0	0.022267443220358805	0.0
2	9.356068579982691E-5	0.0	0.0	0.08981825836783383	0.0
3	9.356068579982691E-5	0.0	0.0	0.1473580801347274	0.0
4	9.356068579982691E-5	0.0	0.0	0.2975229808434496	0.0
5	9.356068579982691E-5	0.0	0.0	0.49736860571187985	0.0
6	9.356068579982691E-5	0.0	0.0	1.039927022665076	0.0
7	9.356068579982691E-5	0.0	0.0	1.2858045049470213	0.0
8	9.356068579982691E-5	0.0	0.0	1.9823638107267327	0.0
9	9.356068579982691E-5	0.0	0.0	2.387575140925783	0.0
10	9.356068579982691E-5	0.0	0.0	3.007508245035436	0.0
11	9.356068579982691E-5	0.0	0.0	3.444249526349028	0.0
12	9.356068579982691E-5	0.0	0.0	3.815872570345941	0.0
13	9.356068579982691E-5	0.0	0.0	4.039576170093326	0.0
14	9.356068579982691E-5	0.0	0.0	4.137908450868945	0.0
15	9.356068579982691E-5	0.0	0.0	4.212569878137207	0.0
16	9.356068579982691E-5	0.0	0.0	4.342619231398966	0.0
17	9.356068579982691E-5	0.0	0.0	4.463218955394943	0.0
18	9.356068579982691E-5	0.0	0.0	4.657544499801183	0.0
19	9.356068579982691E-5	0.0	0.0	4.743620330737024	0.0
20	1.8712137159965383E-4	0.0	0.0	4.837274577222651	0.0
21	1.8712137159965383E-4	0.0	0.0	4.94580497275045	0.0
22	1.8712137159965383E-4	0.0	0.0	5.052370593876453	0.0
23	1.8712137159965383E-4	0.0	0.0	5.183074871938811	0.0
24	1.8712137159965383E-4	0.0	0.0	5.294505648726405	0.0
25	1.8712137159965383E-4	0.0	0.0	5.380581479662246	0.0
26	1.8712137159965383E-4	0.0	0.0	5.462447079737094	0.0
27	1.8712137159965383E-4	0.0	0.0	5.550300563703132	0.0
28	1.8712137159965383E-4	0.0	0.0	5.630575632119384	0.0
29	1.8712137159965383E-4	0.0	0.0	5.722920029003813	0.0
30	1.8712137159965383E-4	0.0	0.0	5.8587701447851614	0.0
31	1.8712137159965383E-4	0.0	0.0	5.961406217107571	0.0
32	2.8068205739948076E-4	0.0	0.0	6.075082450354361	0.0
33	2.8068205739948076E-4	0.0	0.0	6.172666245643581	0.0
34	2.8068205739948076E-4	0.0	0.0	6.276144364138189	0.0
35	2.8068205739948076E-4	0.0	0.0	6.384861881037588	0.0
36	2.8068205739948076E-4	0.0	0.0	6.477299838607817	0.0
37	2.8068205739948076E-4	0.0	0.0	6.582836292190022	0.0
38	2.8068205739948076E-4	0.0	0.0	6.686688653427829	0.0
39	2.8068205739948076E-4	0.0	0.0	6.800645568732019	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	60	0.0	45.000004	1
ACACGAA	25	3.890621E-5	45.000004	42
GTCGACA	20	7.0330634E-4	45.000004	40
AAACGCG	20	7.0330634E-4	45.000004	1
TACTACG	20	7.0330634E-4	45.000004	1
ATCGGTA	20	7.0330634E-4	45.000004	35
ATCGGGT	20	7.0330634E-4	45.000004	4
CAGTACG	40	6.8139343E-9	45.000004	1
TAATGCG	50	2.1827873E-11	45.000004	1
TACACCG	20	7.0330634E-4	45.000004	1
CGCTTAA	20	7.0330634E-4	45.000004	37
GCGTAAG	85	0.0	42.35294	1
GCGATCG	75	0.0	42.0	9
AAGGGTA	570	0.0	41.842106	5
CGTTCGG	65	0.0	41.53846	45
TAGAACG	55	6.184564E-11	40.909092	1
CGAATAT	55	6.184564E-11	40.909092	14
TGCATAG	95	0.0	40.263157	1
ACGTAAG	45	1.9281288E-8	40.0	1
CGTAAGG	210	0.0	39.642857	2
>>END_MODULE