FastQCFastQC Report
Sat 18 Jun 2016
SRR3549290_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549290_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1763616
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.44318037486618406No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48150.2730186162974253No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT34910.19794558452633682No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA17780.10081559704606899No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG207.0343126E-445.0000041
TCGATAG800.045.0000041
ATCGTAC207.0343126E-445.00000431
CCGTATC207.0343126E-445.00000425
TACGCAC207.0343126E-445.00000421
GTTCGAC207.0343126E-445.00000428
TTACGCG406.8193913E-945.0000041
CGCACAT207.0343126E-445.00000431
TGTAACG551.8189894E-1245.00000430
GCGTAAG1100.045.0000041
TACGTTG406.8193913E-945.0000041
AAATGCG551.8189894E-1245.0000041
CGAACCG253.891657E-545.02
TACCACG253.891657E-545.026
TACGCGG700.045.02
TTAACGC253.891657E-545.020
ACCGTAC253.891657E-545.029
ACGTAAC253.891657E-545.040
ATACGAC351.2124292E-745.037
TATACGA351.2124292E-745.036